公开(公告)号：US4912043A

公开(公告)日：1990-03-27

申请号：US864212

申请日：1986-05-19

申请人： Masato Terasawa ,  Terukazu Nara ,  Hideaki Yukawa ,  Hisashi Yamagata ,  Yukie Satoo

发明人： Masato Terasawa ,  Terukazu Nara ,  Hideaki Yukawa ,  Hisashi Yamagata ,  Yukie Satoo

IPC分类号： C12P7/46

CPC分类号： C12P7/46 ,  Y10S435/84

摘要： An aqueous solution containing fumaric acid is reacted in the presence of an aerobically cultured product of a microorganism belonging to the Brevibacterium genus and having resistance to .alpha.-aminobutyric acid, whereby L-malic acid is produced.

摘要翻译： 含有富马酸的水溶液在属于短杆菌属的微生物的有氧培养物的存在下反应并具有对α-氨基丁酸的抗性，从而产生L-苹果酸。

公开(公告)号：US5153123A

公开(公告)日：1992-10-06

申请号：US257524

申请日：1988-10-14

申请人： Masato Terasawa ,  Terukazu Nara ,  Makiko Fukushima ,  Yukie Satoo ,  Mitsunobu Shimazu ,  Hideaki Yukawa ,  Yasurou Kurusu ,  Keiko Kohama

发明人： Masato Terasawa ,  Terukazu Nara ,  Makiko Fukushima ,  Yukie Satoo ,  Mitsunobu Shimazu ,  Hideaki Yukawa ,  Yasurou Kurusu ,  Keiko Kohama

IPC分类号： C12N15/52 ,  C12N15/77 ,  C12P13/08

CPC分类号： C12P13/08 ,  C12N15/52 ,  C12N15/77 ,  Y10S435/84

摘要： A method for producing L-threonine, which comprises subjecting at least L- or DL-aspartic acid or a salt thereof to enzymatic reaction according to the reaction system not accompanied with growth of microorganism cells in an aqueous solution in the presence of a microorganism and collecting L-threonine formed, wherein the microorganism is a biotin-requiring microorganism for the growth belonging to coryneform bacterium; a plasmid comprising a DNA fragment containing at least a gene encoding biosynthesis of threonine which can be expressed within a biotin-requiring microorganism cell for the growth belonging to coryneform bacterium and a DNA fragment containing a gene encoding autonomous replication within coryneform bacterium cell; and a biotin-requiring microorganism for the growth belonging to coryneform bacterium which has been transformed with the plasmid described above, both of which are employed in the present method.According to the present invention, L-threonine can be produced with good yield, and further since production management becomes extremely easy without requiring cumbersome operation such as sterilization of the medium, etc. as in the fermentation method, L-threonine can be produced inexpensively in industry.

公开(公告)号：US5019503A

公开(公告)日：1991-05-28

申请号：US56774

申请日：1987-06-02

申请人： Masato Terasawa ,  Yukie Satoo ,  Hideaki Yukawa

发明人： Masato Terasawa ,  Yukie Satoo ,  Hideaki Yukawa

IPC分类号： C12P41/00 ,  C12N9/90 ,  C12P13/06 ,  C12P13/08 ,  C12R1/13

CPC分类号： C12P13/08 ,  C12N9/90 ,  C12P13/06 ,  Y10S435/84

摘要： A method for producting L-threonine is described, comprising subjecting L- or DL-homoserine to enzymatic reaction in an aqueous medium in the presence of cells of biotin-requiring microorganisms belonging to the genus Brevibacterium and harvesting L-threonine from the reaction mixture.

摘要翻译： 描述了生产L-苏氨酸的方法，包括在属于短杆菌属的生物素需要微生物的细胞的存在下，在水性介质中使L-或DL-高丝氨酸进行酶促反应，并从反应混合物中收获L-苏氨酸。