公开(公告)号：US5185262A

公开(公告)日：1993-02-09

申请号：US473396

申请日：1990-02-01

申请人： Keiko Kohama ,  Miki Kobayashi ,  Yasurou Kurusu ,  Hideaki Yukawa ,  Makiko Fukushima

发明人： Keiko Kohama ,  Miki Kobayashi ,  Yasurou Kurusu ,  Hideaki Yukawa ,  Makiko Fukushima

IPC分类号： C12N9/88 ,  C12N15/68 ,  C12N15/77

CPC分类号： C12N9/88 ,  C12N15/68 ,  C12N15/77

摘要： A DNA fragment derived from plasmid pBY503 obtained from Brevibacterium stationis IF012144, said DNA fragment containing a gene which encodes for the function of maintaining a plasmid, capable of replicating and proliferating at least in a Coryneform bacteria of the genus Brevibacterium, stably in said bacteria; and a vector DNA capable of replicating and proliferating in a Coryneform bacteria into which the above DNA fragment is introduced.

摘要翻译： 从得自短杆菌IF012144的质粒pBY503衍生的DNA片段，所述DNA片段含有能够稳定地在短杆菌中至少在短杆菌属的棒状细菌中复制和增殖的能够维持质粒的功能的基因的基因; 以及能够在导入上述DNA片段的棒状细菌中复制和增殖的载体DNA。

公开(公告)号：US5279951A

公开(公告)日：1994-01-18

申请号：US519776

申请日：1990-05-07

申请人： Masato Terasawa ,  Hisashi Yamagata ,  Hideaki Yukawa ,  Yasurou Kurusu ,  Makiko Fukushima

发明人： Masato Terasawa ,  Hisashi Yamagata ,  Hideaki Yukawa ,  Yasurou Kurusu ,  Makiko Fukushima

IPC分类号： C12P13/22 ,  C12N1/21 ,  C12N9/88 ,  C12N15/09 ,  C12N15/60 ,  C12N15/70 ,  C12R1/19 ,  C12N15/11 ,  C12N15/71

CPC分类号： C12N9/88 ,  C12P13/227 ,  Y10S435/849

摘要： A process for cultivating a microorganism transformed with a recombinant plasmid at least containing (a) a DNA fragment containing a promoter and a regulator gene tnaC located downstream of the promoter in a tryptophanase operon (tna), and (b) a DNA fragment containing a desired structural gene which can be expressed by the promoter, which comprises cultivating the transformed microorganism in a culture medium while adding glucose as a carbon source continuously or intermittently so that the concentration of glucose is maintained within the range of 0.01 to 0.3%, and thereby allowing the desired structural gene to be expressed in the microorganism.

摘要翻译： 培养用重组质粒转化的微生物的方法，所述重组质粒至少含有（a）含有启动子的DNA片段和位于启动子下游的色氨酸酶操纵子（tna）中的调节基因tnaC，和（b）含有 可以由启动子表达的所需结构基因，其包括在连续或间歇地添加作为碳源的葡萄糖的同时在培养基中培养转化的微生物，使得葡萄糖的浓度保持在0.01〜0.3％的范围内，由此 使所需的结构基因在微生物中表达。

公开(公告)号：US5153123A

公开(公告)日：1992-10-06

申请号：US257524

申请日：1988-10-14

申请人： Masato Terasawa ,  Terukazu Nara ,  Makiko Fukushima ,  Yukie Satoo ,  Mitsunobu Shimazu ,  Hideaki Yukawa ,  Yasurou Kurusu ,  Keiko Kohama

发明人： Masato Terasawa ,  Terukazu Nara ,  Makiko Fukushima ,  Yukie Satoo ,  Mitsunobu Shimazu ,  Hideaki Yukawa ,  Yasurou Kurusu ,  Keiko Kohama

IPC分类号： C12N15/52 ,  C12N15/77 ,  C12P13/08

CPC分类号： C12P13/08 ,  C12N15/52 ,  C12N15/77 ,  Y10S435/84

摘要： A method for producing L-threonine, which comprises subjecting at least L- or DL-aspartic acid or a salt thereof to enzymatic reaction according to the reaction system not accompanied with growth of microorganism cells in an aqueous solution in the presence of a microorganism and collecting L-threonine formed, wherein the microorganism is a biotin-requiring microorganism for the growth belonging to coryneform bacterium; a plasmid comprising a DNA fragment containing at least a gene encoding biosynthesis of threonine which can be expressed within a biotin-requiring microorganism cell for the growth belonging to coryneform bacterium and a DNA fragment containing a gene encoding autonomous replication within coryneform bacterium cell; and a biotin-requiring microorganism for the growth belonging to coryneform bacterium which has been transformed with the plasmid described above, both of which are employed in the present method.According to the present invention, L-threonine can be produced with good yield, and further since production management becomes extremely easy without requiring cumbersome operation such as sterilization of the medium, etc. as in the fermentation method, L-threonine can be produced inexpensively in industry.