公开(公告)号：US5153123A

公开(公告)日：1992-10-06

申请号：US257524

申请日：1988-10-14

申请人： Masato Terasawa ,  Terukazu Nara ,  Makiko Fukushima ,  Yukie Satoo ,  Mitsunobu Shimazu ,  Hideaki Yukawa ,  Yasurou Kurusu ,  Keiko Kohama

发明人： Masato Terasawa ,  Terukazu Nara ,  Makiko Fukushima ,  Yukie Satoo ,  Mitsunobu Shimazu ,  Hideaki Yukawa ,  Yasurou Kurusu ,  Keiko Kohama

IPC分类号： C12N15/52 ,  C12N15/77 ,  C12P13/08

CPC分类号： C12P13/08 ,  C12N15/52 ,  C12N15/77 ,  Y10S435/84

摘要： A method for producing L-threonine, which comprises subjecting at least L- or DL-aspartic acid or a salt thereof to enzymatic reaction according to the reaction system not accompanied with growth of microorganism cells in an aqueous solution in the presence of a microorganism and collecting L-threonine formed, wherein the microorganism is a biotin-requiring microorganism for the growth belonging to coryneform bacterium; a plasmid comprising a DNA fragment containing at least a gene encoding biosynthesis of threonine which can be expressed within a biotin-requiring microorganism cell for the growth belonging to coryneform bacterium and a DNA fragment containing a gene encoding autonomous replication within coryneform bacterium cell; and a biotin-requiring microorganism for the growth belonging to coryneform bacterium which has been transformed with the plasmid described above, both of which are employed in the present method.According to the present invention, L-threonine can be produced with good yield, and further since production management becomes extremely easy without requiring cumbersome operation such as sterilization of the medium, etc. as in the fermentation method, L-threonine can be produced inexpensively in industry.

公开(公告)号：US5185262A

公开(公告)日：1993-02-09

申请号：US473396

申请日：1990-02-01

申请人： Keiko Kohama ,  Miki Kobayashi ,  Yasurou Kurusu ,  Hideaki Yukawa ,  Makiko Fukushima

发明人： Keiko Kohama ,  Miki Kobayashi ,  Yasurou Kurusu ,  Hideaki Yukawa ,  Makiko Fukushima

IPC分类号： C12N9/88 ,  C12N15/68 ,  C12N15/77

CPC分类号： C12N9/88 ,  C12N15/68 ,  C12N15/77

摘要： A DNA fragment derived from plasmid pBY503 obtained from Brevibacterium stationis IF012144, said DNA fragment containing a gene which encodes for the function of maintaining a plasmid, capable of replicating and proliferating at least in a Coryneform bacteria of the genus Brevibacterium, stably in said bacteria; and a vector DNA capable of replicating and proliferating in a Coryneform bacteria into which the above DNA fragment is introduced.

摘要翻译： 从得自短杆菌IF012144的质粒pBY503衍生的DNA片段，所述DNA片段含有能够稳定地在短杆菌中至少在短杆菌属的棒状细菌中复制和增殖的能够维持质粒的功能的基因的基因; 以及能够在导入上述DNA片段的棒状细菌中复制和增殖的载体DNA。

公开(公告)号：US5279951A

公开(公告)日：1994-01-18

申请号：US519776

申请日：1990-05-07

申请人： Masato Terasawa ,  Hisashi Yamagata ,  Hideaki Yukawa ,  Yasurou Kurusu ,  Makiko Fukushima

发明人： Masato Terasawa ,  Hisashi Yamagata ,  Hideaki Yukawa ,  Yasurou Kurusu ,  Makiko Fukushima

IPC分类号： C12P13/22 ,  C12N1/21 ,  C12N9/88 ,  C12N15/09 ,  C12N15/60 ,  C12N15/70 ,  C12R1/19 ,  C12N15/11 ,  C12N15/71

CPC分类号： C12N9/88 ,  C12P13/227 ,  Y10S435/849

摘要： A process for cultivating a microorganism transformed with a recombinant plasmid at least containing (a) a DNA fragment containing a promoter and a regulator gene tnaC located downstream of the promoter in a tryptophanase operon (tna), and (b) a DNA fragment containing a desired structural gene which can be expressed by the promoter, which comprises cultivating the transformed microorganism in a culture medium while adding glucose as a carbon source continuously or intermittently so that the concentration of glucose is maintained within the range of 0.01 to 0.3%, and thereby allowing the desired structural gene to be expressed in the microorganism.

摘要翻译： 培养用重组质粒转化的微生物的方法，所述重组质粒至少含有（a）含有启动子的DNA片段和位于启动子下游的色氨酸酶操纵子（tna）中的调节基因tnaC，和（b）含有 可以由启动子表达的所需结构基因，其包括在连续或间歇地添加作为碳源的葡萄糖的同时在培养基中培养转化的微生物，使得葡萄糖的浓度保持在0.01〜0.3％的范围内，由此 使所需的结构基因在微生物中表达。

公开(公告)号：US5597727A

公开(公告)日：1997-01-28

申请号：US245688

申请日：1994-05-18

申请人： Keiko Kohama ,  Kazuhisa Hatakeyama ,  Yasurou Kurusu ,  Hideaki Yukawa

发明人： Keiko Kohama ,  Kazuhisa Hatakeyama ,  Yasurou Kurusu ,  Hideaki Yukawa

IPC分类号： C12N1/21 ,  C12N9/88 ,  C12N15/09 ,  C12N15/69 ,  C12N15/77 ,  C12R1/13 ,  C12R1/15 ,  C07H21/04 ,  C12N15/00 ,  C12N15/74

CPC分类号： C12N9/88 ,  C12N15/69 ,  C12N15/77 ,  Y10S435/84 ,  Y10S435/843

摘要： The present invention relates to a DNA fragment which contains a gene responsible for the function of autonomous replication of plasmid in a Coryneform bacterium, said gene being obtained from plasmid pBY503 held in Brevibacterium stationis, and in which at least one point mutation capable of increasing the copy number of plasmid exists on said gene region. By using a Coryneform transformed with the vector constructed using said DNA fragment and an industrially useful gene such as aspartase gene or tryptophan synthase gene, production of the useful product occurs with higher efficiency than conventional methods.

摘要翻译： 本发明涉及一种DNA片段，其含有负责棒状杆菌型细菌质粒自主复制功能的基因，所述基因得自持有短杆菌属的质粒pBY503，其中能够增加至少一个能够增加 所述基因区上存在质粒的拷贝数。 通过使用由使用所述DNA片段构建的载体和工业上有用的基因如天冬氨酸酶基因或色氨酸合成酶基因转化的棒状细胞，有效产物的产生以比常规方法更高的效率发生。

公开(公告)号：US5738993A

公开(公告)日：1998-04-14

申请号：US662963

申请日：1996-06-13

申请人： Nobutake Fugono ,  Yasurou Kurusu ,  Masato Terasawa ,  Hideaki Yukawa

发明人： Nobutake Fugono ,  Yasurou Kurusu ,  Masato Terasawa ,  Hideaki Yukawa

IPC分类号： C12Q1/68 ,  C07H19/00 ,  C07H21/04

CPC分类号： C12Q1/6876 ,  C12Q1/6827

摘要： An oligonucleotide comprising a specific region and one or two non-specific regions which are bound to at least one of the two termini of the specific region, wherein the specific region has a specific base sequence which is substantially complementary to a target sequence of a sample nucleic acid with which the oligonucleotide is to be hybridized, the non-specific region is composed of at least one nucleotide having other base capable of forming a base pair with each of bases constituting a common nucleic acid, or its oligomer, is used as a probe for the hybridization.

摘要翻译： 包含与特定区域的两个末端中的至少一个结合的特定区域和一个或两个非特异性区域的寡核苷酸，其中特异性区域具有与样品的靶序列基本上互补的特异性碱基序列 寡核苷酸要与其杂交的核酸，非特异性区域由至少一个能够与构成共同核酸的每个碱基形成碱基对的其他碱基的核苷酸或其低聚物用作 探针进行杂交。

公开(公告)号：US5416012A

公开(公告)日：1995-05-16

申请号：US242004

申请日：1994-05-12

申请人： Youichi Niimura ,  Michio Kozaki ,  Hisashi Yamagata ,  Miki Ikuta ,  Yasurou Kurusu ,  Hideaki Yukawa ,  Makiko Kukushima ,  Masato Terasausa

发明人： Youichi Niimura ,  Michio Kozaki ,  Hisashi Yamagata ,  Miki Ikuta ,  Yasurou Kurusu ,  Hideaki Yukawa ,  Makiko Kukushima ,  Masato Terasausa

IPC分类号： C12N1/21 ,  C12N9/02 ,  C12N15/53 ,  C12P1/00 ,  C12P7/40 ,  C12N15/70

CPC分类号： C12N9/0036 ,  C12P1/00 ,  C12P7/40

摘要： A process for producing an NADH oxidase which comprises culturing alkalophilic, facultative anaerobic NADH oxidase-producing bacteria having the optimum growth pH in an alkaline region in medium and collecting said NADH oxidase from the culture; and a plasmid used for the said process.

摘要翻译： 一种NADH氧化酶的制造方法，其特征在于，在培养基中的碱性区域培养具有最佳生长pH的嗜碱性兼性厌氧NADH氧化酶生成菌，从培养物收集所述NADH氧化酶; 和用于所述方法的质粒。