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    Adenovirus library and methods
    1.
    发明授权
    Adenovirus library and methods 有权

    公开(公告)号：US10208304B2

    公开(公告)日：2019-02-19

    申请号：US13447847

    申请日：2012-04-16

    申请人： Masato Yamamoto ,  Yoshiaki Miura

    发明人： Masato Yamamoto ,  Yoshiaki Miura

    IPC分类号： C12N15/10

    摘要： Described herein is a method that generally includes infecting a host cell with a rescue adenovirus, wherein the rescue adenovirus genome comprises a loxP site and encodes at least one marker, and wherein the host cell comprises a library of polynucleotides that complement the adenovirus genome marker and encode a detectable polypeptide; incubating the infected host cell under conditions effective to permit recombination between the adenovirus genome and one or more of the library polynucleotides and the production of recombinant adenovirus particles comprising at least on detectable polypeptide; and detecting the at least one detectable polypeptide. Also described are adenovirus libraries constructed using such a method.

    ADENOVIRUS LIBRARY AND METHODS
    2.
    发明申请
    ADENOVIRUS LIBRARY AND METHODS 审中-公开
    ADENOVIRUS图书馆和方法

    公开(公告)号：US20120264192A1

    公开(公告)日：2012-10-18

    申请号：US13447847

    申请日：2012-04-16

    申请人： MASATO YAMAMOTO ,  Yoshiaki Miura

    发明人： MASATO YAMAMOTO ,  Yoshiaki Miura

    IPC分类号： C12N7/00 ,  C40B40/02 ,  C40B50/06

    CPC分类号： C12N15/1034 ,  C12N15/1037 ,  C12N2710/10043 ,  C12N2710/10071 ,  C12N2800/30

    摘要： Described herein is a method that generally includes infecting a host cell with a rescue adenovirus, wherein the rescue adenovirus genome comprises a loxP site and encodes at least one marker, and wherein the host cell comprises a library of polynucleotides that complement the adenovirus genome marker and encode a detectable polypeptide; incubating the infected host cell under conditions effective to permit recombination between the adenovirus genome and one or more of the library polynucleotides and the production of recombinant adenovirus particles comprising at least on detectable polypeptide; and detecting the at least one detectable polypeptide. Also described are adenovirus libraries constructed using such a method.

    摘要翻译： 本文描述的方法通常包括用救援腺病毒感染宿主细胞，其中所述拯救性腺病毒基因组包含loxP位点并编码至少一种标志物，并且其中所述宿主细胞包含与腺病毒基因组标记物互补的多核苷酸文库，以及 编码可检测的多肽; 在有效地允许腺病毒基因组与一种或多种文库多核苷酸之间重组和产生至少包含可检测多肽的重组腺病毒颗粒的条件下孵育感染的宿主细胞; 以及检测所述至少一种可检测的多肽。 还描述了使用这种方法构建的腺病毒库。

    Method of Esterifying Bio-Related Molecule for Mass Spectrometry and Method of Mass Spectrometry of Obtained Esterified Derivative
    5.
    发明申请
    Method of Esterifying Bio-Related Molecule for Mass Spectrometry and Method of Mass Spectrometry of Obtained Esterified Derivative 有权
    用于质谱分析生物相关分子的方法和获得的酯化衍生物的质谱法

    公开(公告)号：US20090311793A1

    公开(公告)日：2009-12-17

    申请号：US12224310

    申请日：2007-02-22

    申请人： Shin-Ichiro Nishimura ,  Yoshiaki Miura

    发明人： Shin-Ichiro Nishimura ,  Yoshiaki Miura

    IPC分类号： G01N33/68 ,  G01N24/00 ,  G01N33/00

    CPC分类号： G01N33/6848 ,  C07K1/13 ,  Y10T436/143333 ,  Y10T436/24

    摘要： [Problems]To provide a method for enhancing analysis sensitivity of bio-related molecules in mass spectrometry. To provide a method for rapidly and conveniently analyzing biological acid molecules using the method of enhanced analysis sensitivity in mass spectrometry. [Means for Solution] A method of preparing a bio-related molecule to be subjected to mass spectrometry, in which at least a part of acid group(s) is esterified, comprising reacting a bio-related molecule comprising an acid group(s) with a triazene compound to esterify at least a part of said acid group(s). A method of analyzing bio-related molecules comprising reacting a bio-related molecule comprising an acid group(s) with a triazene compound to esterify said acid group(s), and then subjecting said bio-related molecule having an esterified acid group(s) to mass spectrometry.

    摘要翻译： [问题]提供在质谱中提高生物相关分子的分析灵敏度的方法。 提供一种利用质谱法提高分析灵敏度的方法快速方便地分析生物酸分子的方法。 [解决方案]一种制备待进行质谱分析的生物相关分子的方法，其中至少一部分酸基被酯化，包括使包含酸基的生物相关分子， 与三氮烯化合物酯化至少一部分所述酸基团。 一种分析生物相关分子的方法，包括使包含酸基团的生物相关分子与三氮烯化合物反应以使所述酸基团酯化，然后使具有酯化酸基团的生物相关分子 ）进行质谱分析。

    Novel resin composition and chassis for electronic instruments shaped therefrom
    8.
    发明授权
    Novel resin composition and chassis for electronic instruments shaped therefrom 失效
    用于从其形成的电子仪器的新型树脂组合物和底盘

    公开(公告)号：US5082892A

    公开(公告)日：1992-01-21

    申请号：US485222

    申请日：1990-02-26

    申请人： Yoshiaki Miura ,  Masami Mihara ,  Hidezo Hosomi

    发明人： Yoshiaki Miura ,  Masami Mihara ,  Hidezo Hosomi

    IPC分类号： C08L25/08 ,  C08L35/06 ,  H05K5/02

    CPC分类号： H05K5/02 ,  C08L35/06 ,  C08L25/08

    摘要： A glass fiber-reinforced synthetic resin composition is proposed which is particularly suitable as a molding material of chassis of electric and electronic instruments to satisfy the various requirements in this particular application. The resin composition comprises: (A) 100 parts by weight of a copolymeric resin of styrene and maleic anhydride or a derivative thereof, of which the molar fraction of the moiety of maleic anhydride or a derivative thereof is in the range from 3 to 20%; (B) from 1 to 30 parts by weight of a styrene-based thermoplastic elastomer, of which the weight fraction of the moiety of styrene is in the range from 5 to 50%; (C) from 5 to 80 parts by weight of glass fibers, (D) from 5 to 30 parts by weight of a bromine-containing organic compound as a flame retardant; and (E) from 2 to 15 parts by weight of antimony trioxide.

    摘要翻译： 提出一种玻璃纤维增​​强合成树脂组合物，其特别适用于电气和电子仪器底盘的成型材料，以满足该特定应用中的各种要求。 树脂组合物包含：（A）100重量份的苯乙烯和马来酸酐或其衍生物的共聚树脂，其马来酐或其衍生物部分的摩尔分数为3-20重量％ ; （B）1〜30重量份苯乙烯类热塑性弹性体，其中苯乙烯部分的重量分数为5〜50％的范围; （C）5〜80重量份玻璃纤维，（D）5〜30重量份作为阻燃剂的含溴有机化合物; 和（E）2至15重量份的三氧化锑。

    Apparatus for auto-pretreating sugar chain
    9.
    发明授权
    Apparatus for auto-pretreating sugar chain 有权
    用于自动预处理糖链的装置

    公开(公告)号：US08877454B2

    公开(公告)日：2014-11-04

    申请号：US12681573

    申请日：2008-10-03

    申请人： Shinichiro Nishimura ,  Yasuro Shinohara ,  Yoshiaki Miura ,  Hiroshi Yamazaki ,  Michio Horiuchi ,  Hiroaki Motoki ,  Toshiharu Kuroda ,  Yoko Kita ,  Mika Nakano

    发明人： Shinichiro Nishimura ,  Yasuro Shinohara ,  Yoshiaki Miura ,  Hiroshi Yamazaki ,  Michio Horiuchi ,  Hiroaki Motoki ,  Toshiharu Kuroda ,  Yoko Kita ,  Mika Nakano

    IPC分类号： C12Q1/37 ,  G01N33/68 ,  G01N30/72 ,  H01J49/00

    CPC分类号： G01N33/6848 ,  G01N30/7233 ,  G01N2400/00 ,  H01J49/00

    摘要： To provide an autoanalyzer for analyzing a sugar chain contained in a biological sample, in particular, serum. Namely, it is intended to provide a method of analyzing a sugar chain in a sample, which comprises the following steps: A) the sugar chain-releasing step of releasing the sugar chain in the sample; B) the detection sample-preparing step of preparing the released sugar chain for detection; and, in the case of conducting mass spectrometry using a plate, C) the step of forming a plate for the mass spectrometry having the captured sugar chain dotted thereon which comprises the step of providing the tagged sugar chain sample solution obtained in the step B) on a collection plate; and, if required, the step of conducting an operation in a solid phase support-enclosed plate to form the plate for mass spectrometry; and D) the step of analyzing the sugar chain to be assayed.

    摘要翻译： 提供一种用于分析生物样品，特别是血清中所含的糖链的自动分析仪。 即，提供一种分析样品中的糖链的方法，其包括以下步骤：A）释放样品中的糖链的糖链释放步骤; B）制备释放的糖链进行检测的检测样品制备步骤; 并且在使用板进行质谱分析的情况下，C）形成具有点状捕获的糖链的用于质谱的板的步骤，其包括提供步骤B）中获得的标记的糖链样品溶液的步骤， 在收集板上; 如果需要，在固相支持封闭板中进行操作以形成质谱板的步骤; 和D）分析要测定的糖链的步骤。