公开(公告)号：US20090130022A1

公开(公告)日：2009-05-21

申请号：US11916391

申请日：2006-06-06

申请人： Junji Nishigaki ,  Hiroyuki Hirai ,  Masayoshi Kojima ,  Kazuhiro Aikawa

发明人： Junji Nishigaki ,  Hiroyuki Hirai ,  Masayoshi Kojima ,  Kazuhiro Aikawa

IPC分类号： A61B5/055 ,  A61K9/127

CPC分类号： A61K49/1812

摘要： A liposome containing a phosphatidylcholine and a phosphatidylserine in combination as membrane components at a phosphatidylcholine:phosphatidylserine molar ratio of 3:1 to 1:2, and said liposome containing superparamagnetic particles having a mean particle size not less than 1 nm and not more than 50 nm. A means for selectively accumulating an MRI contrast medium in a lesion of a vascular disease caused by abnormal proliferation of vascular smooth muscle cells such as arteriosclerosis and restenosis after PTCA is provided.

摘要翻译： 含有磷脂酰胆碱和磷脂酰丝氨酸的磷脂酰丝氨酸组合为磷脂酰胆碱的膜组分：磷脂酰丝氨酸摩尔比为3:1至1:2，所述脂质体含有平均粒度不小于1nm且不大于50的超顺磁性颗粒 nm。 用于选择性地累积由血管平滑肌细胞的异常增殖引起的血管疾病的病变中的MRI造影剂的手段，所述血管病变由PTCA提供之后的动脉硬化和再狭窄引起。

公开(公告)号：US20080156736A1

公开(公告)日：2008-07-03

申请号：US11883742

申请日：2006-02-08

申请人： Hiroyuki Hirai ,  Masayoshi Kojima

发明人： Hiroyuki Hirai ,  Masayoshi Kojima

IPC分类号： B03C1/02 ,  H01F1/42

CPC分类号： B03C1/01 ,  B82Y25/00 ,  H01F1/0054

摘要： A magnetic composite body comprising magnetic nanoparticles having a number average particle diameter of 1 to 50 nm and having a compound represented by formula (I) fixed on their surfaces, and a method comprising bringing the magnetic composite body into contact with a test piece followed by magnetic separation: R1O—(CH(R2)CH2O)n-L-X   Formula (I) wherein R1 represents a hydrogen atom, an alkyl or alkenyl group having a carbon chain length of 1 to 20, or a substituted or unsubstituted, aryl or heterocyclic group; R2 represents a hydrogen atom or a methyl group; L may be present or not present, and, when L is present, L represents an alkylene or alkenylene group having a carbon chain length of 1 to 10 which may have a branched chain or a substituent; X represents a hydrogen atom, a carboxylic acid group, a phosphoric acid group, or a sulfonic acid group; and n represents an integer of 1 to 10

摘要翻译： 一种磁性复合体，其包含数均粒径为1〜50nm的磁性纳米颗粒，并且具有固定在其表面上的式（I）表示的化合物，以及包括使磁性复合体与试验片接触的方法， 磁分离：<？in-line-formula description =“In-line Formulas”end =“lead”？> R O-（CH（R 2）2 CH 式（I）<？in-line-formula description =“In-line formula”end =“tail”？>其中R ^{1 表示氢原子，碳链长度为1-20的烷基或烯基，或取代或未取代的芳基或杂环基; R 2表示氢原子或甲基; L可以存在或不存在，并且当存在L时，L表示碳链长度为1至10的亚烷基或亚烯基，其可以具有支链或取代基; X表示氢原子，羧酸基，磷酸基或磺酸基; n表示1〜10的整数}

公开(公告)号：US08247241B2

公开(公告)日：2012-08-21

申请号：US11723634

申请日：2007-03-21

申请人： Hiroyuki Hirai ,  Masayoshi Kojima ,  Isao Tsuyuki

发明人： Hiroyuki Hirai ,  Masayoshi Kojima ,  Isao Tsuyuki

IPC分类号： G01N33/553

CPC分类号： G01N33/54326

摘要： According to an aspect of the invention, there is provided a method for detecting a target compound including: injecting a test liquid containing a target compound into a colloid solution of magnetic nano-particles having an average particle diameter of 50 nm or less to allow the target compound to bind to the magnetic nano-particles, thereby forming bound magnetic nano-particles having a diameter of 100 nm or more; and bringing a dispersion liquid containing the bound magnetic nano-particles in proximity to a magnetic sensor including at least a magnetoresistive (MR) element and a permanent magnet while measuring the change in magnetic resistance to selectively detect the bound magnetic nano-particles, thereby indirectly detecting the target compound.

摘要翻译： 根据本发明的一个方面，提供了一种检测目标化合物的方法，包括：将含有目标化合物的测试液体注入到平均粒径为50nm或更小的磁性纳米颗粒的胶体溶液中， 目标化合物结合磁性纳米粒子，从而形成直径为100nm以上的结合磁性纳米粒子; 并将包含结合的磁性纳米颗粒的分散液在包括至少磁阻（MR）元件和永磁体的磁性传感器附近，同时测量磁阻的变化，从而间接地选择性地检测结合的磁性纳米颗粒 检测目标化合物。

公开(公告)号：US20080032415A1

公开(公告)日：2008-02-07

申请号：US11630199

申请日：2005-06-13

申请人： Junji Nishigaki ,  Masayoshi Kojima ,  Hiroyuki Hirai

发明人： Junji Nishigaki ,  Masayoshi Kojima ,  Hiroyuki Hirai

IPC分类号： G01N21/64

CPC分类号： G01N21/6428 ,  A61B2090/3941 ,  A61B2090/395 ,  G01N2021/6432 ,  G01N2021/6441

摘要： Two or more target substances are simply and efficiently detected using one type of nanoparticle. Two or more target substances contained in one sample are labeled with two or more fluorescent dyes each capable of binding to the respective target substance and each of which emit light at wavelengths different from each other, combination is made with a phosphor nano-particle capable of binding to each of the two or more fluorescent dyes, subsequently irradiation is undertaken with an excitation light which excites the phosphor nano-particle to emit light, and the two or more fluorescent dyes are caused to emit fluorescence using the light from the phosphor nano-particle as an excitation light.

摘要翻译： 使用一种类型的纳米颗粒简单有效地检测两种或更多种靶物质。 包含在一个样品中的两种或更多种目标物质用两种或更多种荧光染料标记，每种荧光染料能够结合相应的目标物质，并且每个荧光染料发射彼此不同的波长的光， 结合两种或更多种荧光染料中的每种，随后用激发荧光体纳米颗粒发光的激发光进行照射，并且使用来自荧光体纳米颗粒的光使两种或更多种荧光染料发射荧光， 颗粒作为激发光。

公开(公告)号：US20070224705A1

公开(公告)日：2007-09-27

申请号：US11723634

申请日：2007-03-21

申请人： Hiroyuki Hirai ,  Masayoshi Kojima ,  Isao Tsuyuki

发明人： Hiroyuki Hirai ,  Masayoshi Kojima ,  Isao Tsuyuki

IPC分类号： G01N33/553

CPC分类号： G01N33/54326

摘要： According to an aspect of the invention, there is provided a method for detecting a target compound including: injecting a test liquid containing a target compound into a colloid solution of magnetic nano-particles having an average particle diameter of 50 nm or less to allow the target compound to bind to the magnetic nano-particles, thereby forming bound magnetic nano-particles having a diameter of 100 nm or more; and bringing a dispersion liquid containing the bound magnetic nano-particles in proximity to a magnetic sensor including at least a magnetoresistive (MR) element and a permanent magnet while measuring the change in magnetic resistance to selectively detect the bound magnetic nano-particles, thereby indirectly detecting the target compound.

摘要翻译： 根据本发明的一个方面，提供了一种检测目标化合物的方法，包括：将含有目标化合物的测试液体注入到平均粒径为50nm或更小的磁性纳米颗粒的胶体溶液中， 目标化合物结合磁性纳米粒子，从而形成直径为100nm以上的结合磁性纳米粒子; 并将包含结合的磁性纳米颗粒的分散液在包括至少磁阻（MR）元件和永磁体的磁性传感器附近，同时测量磁阻的变化，从而间接地选择性地检测结合的磁性纳米颗粒 检测目标化合物。

公开(公告)号：US20060194887A1

公开(公告)日：2006-08-31

申请号：US11362055

申请日：2006-02-27

申请人： Masayoshi Kojima ,  Hiroyuki Hirai

发明人： Masayoshi Kojima ,  Hiroyuki Hirai

IPC分类号： B01F17/00 ,  C09K3/00

CPC分类号： B01F17/0021 ,  B01F17/0028

摘要： The present invention provides a method of controlling aggregation and dispersion of magnetic nano particles, the method including changing, in a sample liquid containing independently dispersed magnetic nano particles having a particle size of 1 to 50 nm, at least one condition selected from the type of a salt present as a medium, the concentration of the salt, and the pH of the sample liquid to thereby control aggregation and dispersion of the magnetic nano particles.

摘要翻译： 本发明提供了一种控制磁性纳米颗粒的聚集和分散的方法，该方法包括在含有独立分散的粒度为1至50nm的磁性纳米颗粒的样品液中改变至少一种选自以下的条件： 作为介质存在的盐，盐的浓度和样品液体的pH，从而控制磁性纳米颗粒的聚集和分散。

公开(公告)号：US20090074671A1

公开(公告)日：2009-03-19

申请号：US12293331

申请日：2007-03-30

申请人： Masayoshi Kojima ,  Hiroyuki Hirai

发明人： Masayoshi Kojima ,  Hiroyuki Hirai

IPC分类号： A61K9/14 ,  A61K49/18

CPC分类号： A61K9/5094 ,  A61K9/0009 ,  A61K9/5115 ,  A61K41/0052 ,  A61K47/6923 ,  A61K49/1833 ,  A61K49/1836 ,  A61K49/1866 ,  A61K49/1869 ,  B82Y5/00 ,  G01N33/54346

摘要： An object of the present invention is to provide a composite of an inorganic nanoparticle with a medicinal compound (i.e., an active substance), and to provide a method for producing the inorganic nanoparticle composite, which is highly versatile and convenient. The present invention provides an active substance-immobilized inorganic nanoparticle wherein an active substance is immobilized through physical adsorption on the surface of an inorganic nanoparticle of 1 to 500 nm in average particle size.

摘要翻译： 本发明的目的是提供一种无机纳米粒子与药用化合物（即活性物质）的复合体，提供一种高度通用性和方便性的无机纳米粒子复合体的制造方法。 本发明提供了一种活性物质固定化的无机纳米粒子，其中通过物理吸附将平均粒度为1〜500nm的无机纳米粒子的表面固定活性物质。

公开(公告)号：US20090029481A1

公开(公告)日：2009-01-29

申请号：US11919869

申请日：2006-06-29

申请人： Masayoshi Kojima ,  Hiroyuki Hirai ,  Shigeki Kageyama ,  Yoshihiko Abe

发明人： Masayoshi Kojima ,  Hiroyuki Hirai ,  Shigeki Kageyama ,  Yoshihiko Abe

IPC分类号： G01N33/48 ,  B01J19/08

CPC分类号： G01N33/5434 ,  G01N33/92 ,  G01N2446/80

摘要： An object to be achieved by the present invention is to provide a method for determining the amount of a component contained in a specific lipoprotein fraction in a biological sample using an automatic analyzer, without the requirement of a step of fractionating a sample by centrifugation. The present invention provides a method for separating a target component in a biological sample, which comprises the steps of: (1) causing a biological sample to come into contact with independently dispersed magnetic nanoparticles having a particle size of 50 nm or less, which have anionic functional groups on their surfaces, so as to form an agglutinate of the magnetic nanoparticles and biomolecules capable of interacting with the magnetic nanoparticles; and (2) collecting the agglutinate by an external magnetic field.

摘要翻译： 本发明要实现的目的是提供一种使用自动分析仪测定生物样品中特定脂蛋白部分中所含成分的量的方法，而不需要通过离心分离样品的步骤。 本发明提供一种用于分离生物样品中的目标成分的方法，其包括以下步骤：（1）使生物样品与粒径为50nm以下的独立分散的磁性纳米粒子接触， 在其表面上具有阴离子官能团，以便形成能够与磁性纳米颗粒相互作用的磁性纳米颗粒和生物分子的凝集物; 和（2）通过外部磁场收集凝集物。

公开(公告)号：US08084275B2

公开(公告)日：2011-12-27

申请号：US11883742

申请日：2006-02-08

申请人： Hiroyuki Hirai ,  Masayoshi Kojima

发明人： Hiroyuki Hirai ,  Masayoshi Kojima

IPC分类号： G01N33/553 ,  B01D35/06 ,  G01N25/18

CPC分类号： B03C1/01 ,  B82Y25/00 ,  H01F1/0054

摘要： A magnetic composite body comprising magnetic nanoparticles having a number average particle diameter of 1 to 50 nm and having a compound represented by formula (I) fixed on their surfaces, and a method comprising bringing the magnetic composite body into contact with a test piece followed by magnetic separation: R1O—(CH(R2)CH2O)n-L-X   Formula (I) wherein R1 represents a hydrogen atom, an alkyl or alkenyl group having a carbon chain length of 1 to 20, or a substituted or unsubstituted, aryl or heterocyclic group; R2 represents a hydrogen atom or a methyl group; L may be present or not present, and, when L is present, L represents an alkylene or alkenylene group having a carbon chain length of 1 to 10 which may have a branched chain or a substituent; X represents a hydrogen atom, a carboxylic acid group, a phosphoric acid group, or a sulfonic acid group; and n represents an integer of 1 to 10.

摘要翻译： 一种磁性复合体，其包含数均粒径为1〜50nm的磁性纳米颗粒，并且具有固定在其表面上的式（I）表示的化合物，以及包括使磁性复合体与试验片接触的方法， 磁性分离：R1O-（CH（R2）CH2O）nLX其中R1表示氢原子，碳链长度为1-20的烷基或烯基或取代或未取代的芳基或杂环基的式（I） R2表示氢原子或甲基; L可以存在或不存在，并且当存在L时，L表示碳链长度为1至10的亚烷基或亚烯基，其可以具有支链或取代基; X表示氢原子，羧酸基，磷酸基或磺酸基; n表示1〜10的整数。

公开(公告)号：US20080020483A1

公开(公告)日：2008-01-24

申请号：US11632229

申请日：2005-07-14

申请人： Junji Nishigaki ,  Masayoshi Kojima ,  Hiroyuki Hirai ,  Tatsuya Takahashi

发明人： Junji Nishigaki ,  Masayoshi Kojima ,  Hiroyuki Hirai ,  Tatsuya Takahashi

IPC分类号： G01N21/64

CPC分类号： G01N21/6428 ,  B82Y30/00 ,  C01G9/02 ,  C01P2002/54 ,  C01P2004/64 ,  C01P2006/22 ,  C09K11/025 ,  C09K11/54

摘要： The invention provides a fluorescence detecting method, comprising irradiating an excitation light in the UV range on a sample containing a nanoparticle fluorescent material having an emission half-value width of 50 nm to 200 nm as the fluorescent material and detecting the fluorescence emitted from the sample in the visible range.

摘要翻译： 本发明提供一种荧光检测方法，其包括在含有发光半值宽度为50nm至200nm的纳米颗粒荧光材料的样品上照射UV范围内的激发光作为荧光材料，并检测从样品发出的荧光 在可见范围内。