公开(公告)号：US20080020483A1

公开(公告)日：2008-01-24

申请号：US11632229

申请日：2005-07-14

申请人： Junji Nishigaki ,  Masayoshi Kojima ,  Hiroyuki Hirai ,  Tatsuya Takahashi

发明人： Junji Nishigaki ,  Masayoshi Kojima ,  Hiroyuki Hirai ,  Tatsuya Takahashi

IPC分类号： G01N21/64

CPC分类号： G01N21/6428 ,  B82Y30/00 ,  C01G9/02 ,  C01P2002/54 ,  C01P2004/64 ,  C01P2006/22 ,  C09K11/025 ,  C09K11/54

摘要： The invention provides a fluorescence detecting method, comprising irradiating an excitation light in the UV range on a sample containing a nanoparticle fluorescent material having an emission half-value width of 50 nm to 200 nm as the fluorescent material and detecting the fluorescence emitted from the sample in the visible range.

摘要翻译： 本发明提供一种荧光检测方法，其包括在含有发光半值宽度为50nm至200nm的纳米颗粒荧光材料的样品上照射UV范围内的激发光作为荧光材料，并检测从样品发出的荧光 在可见范围内。

公开(公告)号：US20070015226A1

公开(公告)日：2007-01-18

申请号：US11483103

申请日：2006-07-10

申请人： Hiroyuki Hirai ,  Masayoshi Kojima ,  Junji Nishigaki

发明人： Hiroyuki Hirai ,  Masayoshi Kojima ,  Junji Nishigaki

IPC分类号： G01N33/574 ,  C07K16/46

CPC分类号： C07K16/2863 ,  C07K2317/55 ,  G01N33/533 ,  G01N33/553

摘要： It is an object of the present invention to provide a nanoparticle fluorescent material for detecting cancer with which cancer can be detected with high sensitivity, using highly safe and broad light emission on a simple device. The present invention provides a nanoparticle fluorescent material which comprises a metal-oxide or metal-sulfide nanoparticle fluorescent material whose surface is modified by a surface modifying agent and whose half bandwidth of light emission is between 50 and 200 nm, wherein an antibody that recognizes cancer antigen is bound to the surface modifying agent.

摘要翻译： 本发明的目的是提供一种用于检测癌症的纳米颗粒荧光材料，通过在简单的装置上使用高度安全和宽泛的发光，可高灵敏度地检测癌症。 本发明提供一种纳米颗粒荧光材料，其包含金属氧化物或金属硫化物纳米颗粒荧光材料，其表面被表面改性剂改性，其半光带宽为50-200nm，其中识别癌症的抗体 抗原与表面改性剂结合。

公开(公告)号：US20090130022A1

公开(公告)日：2009-05-21

申请号：US11916391

申请日：2006-06-06

申请人： Junji Nishigaki ,  Hiroyuki Hirai ,  Masayoshi Kojima ,  Kazuhiro Aikawa

发明人： Junji Nishigaki ,  Hiroyuki Hirai ,  Masayoshi Kojima ,  Kazuhiro Aikawa

IPC分类号： A61B5/055 ,  A61K9/127

CPC分类号： A61K49/1812

摘要： A liposome containing a phosphatidylcholine and a phosphatidylserine in combination as membrane components at a phosphatidylcholine:phosphatidylserine molar ratio of 3:1 to 1:2, and said liposome containing superparamagnetic particles having a mean particle size not less than 1 nm and not more than 50 nm. A means for selectively accumulating an MRI contrast medium in a lesion of a vascular disease caused by abnormal proliferation of vascular smooth muscle cells such as arteriosclerosis and restenosis after PTCA is provided.

摘要翻译： 含有磷脂酰胆碱和磷脂酰丝氨酸的磷脂酰丝氨酸组合为磷脂酰胆碱的膜组分：磷脂酰丝氨酸摩尔比为3:1至1:2，所述脂质体含有平均粒度不小于1nm且不大于50的超顺磁性颗粒 nm。 用于选择性地累积由血管平滑肌细胞的异常增殖引起的血管疾病的病变中的MRI造影剂的手段，所述血管病变由PTCA提供之后的动脉硬化和再狭窄引起。

公开(公告)号：US20080032415A1

公开(公告)日：2008-02-07

申请号：US11630199

申请日：2005-06-13

申请人： Junji Nishigaki ,  Masayoshi Kojima ,  Hiroyuki Hirai

发明人： Junji Nishigaki ,  Masayoshi Kojima ,  Hiroyuki Hirai

IPC分类号： G01N21/64

CPC分类号： G01N21/6428 ,  A61B2090/3941 ,  A61B2090/395 ,  G01N2021/6432 ,  G01N2021/6441

摘要： Two or more target substances are simply and efficiently detected using one type of nanoparticle. Two or more target substances contained in one sample are labeled with two or more fluorescent dyes each capable of binding to the respective target substance and each of which emit light at wavelengths different from each other, combination is made with a phosphor nano-particle capable of binding to each of the two or more fluorescent dyes, subsequently irradiation is undertaken with an excitation light which excites the phosphor nano-particle to emit light, and the two or more fluorescent dyes are caused to emit fluorescence using the light from the phosphor nano-particle as an excitation light.

摘要翻译： 使用一种类型的纳米颗粒简单有效地检测两种或更多种靶物质。 包含在一个样品中的两种或更多种目标物质用两种或更多种荧光染料标记，每种荧光染料能够结合相应的目标物质，并且每个荧光染料发射彼此不同的波长的光， 结合两种或更多种荧光染料中的每种，随后用激发荧光体纳米颗粒发光的激发光进行照射，并且使用来自荧光体纳米颗粒的光使两种或更多种荧光染料发射荧光， 颗粒作为激发光。

公开(公告)号：US06642375B2

公开(公告)日：2003-11-04

申请号：US09731279

申请日：2000-12-06

申请人： Hiroko Inomata ,  Hiroshi Shinoki ,  Masayoshi Kojima ,  Yukio Sudo ,  Junji Nishigaki ,  Osamu Seshimoto

发明人： Hiroko Inomata ,  Hiroshi Shinoki ,  Masayoshi Kojima ,  Yukio Sudo ,  Junji Nishigaki ,  Osamu Seshimoto

IPC分类号： C07H1904

CPC分类号： G01N33/582

摘要： The present invention provides a fluorescent substance which is represented by a formula: A-B-C wherein A is a residue of natural or synthetic nucleotide, oligonucleotide, polynucleotide, or derivative thereof, and binds to B at a base moiety in said residue, or A is a residue of avidin or streptavidin; B is a divalent linking group or a single bond; and C is a monovalent group derived from a general formula (I) and binds to B at a reactive group present in R1 or R2: wherein R1 and R2 each independently represent an alkyl group that may be substituted with a reactive group capable of covalently bonding to A-B-; R3, R4, R5, and R6 each independently represent an alkyl group, and R3 and R4, and/or R5 and R6 may bind to each other to form a saturated carbon-ring together with a carbon atom(s) to which they bind; V1, V2, V3, V4, V5, V6, V7, V8, V9 and V10 each independently represent a hydrogen atom or a monovalent substituent, and two adjacent groups thereof may bind to form a ring; L1, L2, and L3 represent a substituted or unsubstituted methine group; each of m, n, s, and t represents 0 or 1, provided that m+n=1 and s+t=1; p represents 1, 2, or 3; M represents a counter ion; and q represents a number required to neutralize the charge of a molecule. The fluorescent substance of the present invention is useful as a labeling substance for nucleic acids, or as a reagent for analyzing biological components such as nucleic acids, proteins or sugars.

摘要翻译： 本发明提供一种荧光物质，其由下式表示：AB-C，其中A是天然或合成的核苷酸，寡核苷酸，多核苷酸或其衍生物的残基，并与所述残基中的碱基部分的B结合，或A是 抗生物素蛋白或链霉亲和素的残基; B是二价连接基团或单键; 和C是衍生自通式（I）的一价基团，并且在R 1或R 2中存在的反应性基团上与B结合：其中R 1和R 2各自独立地表示烷基， 可以被能够与AB-共价结合的反应性基团取代; R 3，R 4，R 5和R 6各自独立地表示烷基，R 3和R 4和/或R 5和R 6独立地表示烷基， 可以彼此结合形成饱和碳环与它们所结合的碳原子一起; V 1，V 2，V 3，V 4，V 5，V 6，V 7，V 8，V 9和V 10 独立地表示氢原子或一价取代基，其两个相邻基团可以结合形成环; L 1，L 2和L 3表示取代或未取代的次甲基; m，n，s和t中的每一个表示0或1，条件是m + n = 1且s + t = 1; p表示1,2或3; M表示抗衡离子; q表示中和分子电荷所需的数。 本发明的荧光物质可用作核酸的标记物质，或用作分析核酸，蛋白质或糖类等生物成分的试剂。

公开(公告)号：US07087744B2

公开(公告)日：2006-08-08

申请号：US10196223

申请日：2002-07-17

申请人： Junji Nishigaki ,  Kouki Nakamura ,  Kazuya Takeuchi ,  Hiroko Inomata ,  Masayoshi Kojima

发明人： Junji Nishigaki ,  Kouki Nakamura ,  Kazuya Takeuchi ,  Hiroko Inomata ,  Masayoshi Kojima

IPC分类号： C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/68 ,  C12P19/34

CPC分类号： C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C12Q2563/107

摘要： Fluorescent nucleotides useful for labeling nucleic acids which are represented by the formula: X—Y—Z wherein X represents a residue of natural or non-natural nucleotide and the like and binds to Y at a basic moiety of the residue; Y represents a divalent bridging group or a single bond; Z represents a monovalent group derived from a compound represented by the formula (I) wherein R1 to R4 represent an alkyl group; V1 to V6 represent a hydrogen atom or a substituent; L1 to L7 represent a methine group; W represents an oxygen atom, a sulfur atom, —C(R3)(R4)— or —N(R5)— wherein R3 to R5 represents an alkyl group; Q represents a nitrogen atom or —C(V7)— wherein V7 represents a hydrogen atom or a monovalent substituent; M represents a counter ion; m represents a number required to neutralize the charge of the molecule; s, t and u represent 0 or 1 or the like, and binds to Y at a reactive group existing in R1 or R2

摘要翻译： 用于标记由式X-Y-Z表示的核酸的荧光核苷酸，其中X表示天然或非天然核苷酸的残基等，并与残基的碱性部分结合; Y表示二价桥基或单键; Z表示由式（I）表示的化合物衍生的单价基团，其中R 1至R 4代表烷基; V 1至V 6表示氢原子或取代基; L 1至L 7表示次甲基; W表示氧原子，硫原子，-C（R 3）（R 4） - 或-N（R 5） - 其中R 3至R 5表示烷基; Q表示氮原子或-C（V-7） - ，其中V 7表示氢原子或一价取代基; M表示抗衡离子; m表示中和分子电荷所需的数量; s，t和u表示0或1等，并且在R 1或R 2中存在的反应性基团与Y结合，

公开(公告)号：US08084275B2

公开(公告)日：2011-12-27

申请号：US11883742

申请日：2006-02-08

申请人： Hiroyuki Hirai ,  Masayoshi Kojima

发明人： Hiroyuki Hirai ,  Masayoshi Kojima

IPC分类号： G01N33/553 ,  B01D35/06 ,  G01N25/18

CPC分类号： B03C1/01 ,  B82Y25/00 ,  H01F1/0054

摘要： A magnetic composite body comprising magnetic nanoparticles having a number average particle diameter of 1 to 50 nm and having a compound represented by formula (I) fixed on their surfaces, and a method comprising bringing the magnetic composite body into contact with a test piece followed by magnetic separation: R1O—(CH(R2)CH2O)n-L-X   Formula (I) wherein R1 represents a hydrogen atom, an alkyl or alkenyl group having a carbon chain length of 1 to 20, or a substituted or unsubstituted, aryl or heterocyclic group; R2 represents a hydrogen atom or a methyl group; L may be present or not present, and, when L is present, L represents an alkylene or alkenylene group having a carbon chain length of 1 to 10 which may have a branched chain or a substituent; X represents a hydrogen atom, a carboxylic acid group, a phosphoric acid group, or a sulfonic acid group; and n represents an integer of 1 to 10.

摘要翻译： 一种磁性复合体，其包含数均粒径为1〜50nm的磁性纳米颗粒，并且具有固定在其表面上的式（I）表示的化合物，以及包括使磁性复合体与试验片接触的方法， 磁性分离：R1O-（CH（R2）CH2O）nLX其中R1表示氢原子，碳链长度为1-20的烷基或烯基或取代或未取代的芳基或杂环基的式（I） R2表示氢原子或甲基; L可以存在或不存在，并且当存在L时，L表示碳链长度为1至10的亚烷基或亚烯基，其可以具有支链或取代基; X表示氢原子，羧酸基，磷酸基或磺酸基; n表示1〜10的整数。

公开(公告)号：US20080156736A1

公开(公告)日：2008-07-03

申请号：US11883742

申请日：2006-02-08

申请人： Hiroyuki Hirai ,  Masayoshi Kojima

发明人： Hiroyuki Hirai ,  Masayoshi Kojima

IPC分类号： B03C1/02 ,  H01F1/42

CPC分类号： B03C1/01 ,  B82Y25/00 ,  H01F1/0054

摘要： A magnetic composite body comprising magnetic nanoparticles having a number average particle diameter of 1 to 50 nm and having a compound represented by formula (I) fixed on their surfaces, and a method comprising bringing the magnetic composite body into contact with a test piece followed by magnetic separation: R1O—(CH(R2)CH2O)n-L-X   Formula (I) wherein R1 represents a hydrogen atom, an alkyl or alkenyl group having a carbon chain length of 1 to 20, or a substituted or unsubstituted, aryl or heterocyclic group; R2 represents a hydrogen atom or a methyl group; L may be present or not present, and, when L is present, L represents an alkylene or alkenylene group having a carbon chain length of 1 to 10 which may have a branched chain or a substituent; X represents a hydrogen atom, a carboxylic acid group, a phosphoric acid group, or a sulfonic acid group; and n represents an integer of 1 to 10

摘要翻译： 一种磁性复合体，其包含数均粒径为1〜50nm的磁性纳米颗粒，并且具有固定在其表面上的式（I）表示的化合物，以及包括使磁性复合体与试验片接触的方法， 磁分离：<？in-line-formula description =“In-line Formulas”end =“lead”？> R O-（CH（R 2）2 CH 式（I）<？in-line-formula description =“In-line formula”end =“tail”？>其中R ^{1 表示氢原子，碳链长度为1-20的烷基或烯基，或取代或未取代的芳基或杂环基; R 2表示氢原子或甲基; L可以存在或不存在，并且当存在L时，L表示碳链长度为1至10的亚烷基或亚烯基，其可以具有支链或取代基; X表示氢原子，羧酸基，磷酸基或磺酸基; n表示1〜10的整数}

公开(公告)号：US08247241B2

公开(公告)日：2012-08-21

申请号：US11723634

申请日：2007-03-21

申请人： Hiroyuki Hirai ,  Masayoshi Kojima ,  Isao Tsuyuki

发明人： Hiroyuki Hirai ,  Masayoshi Kojima ,  Isao Tsuyuki

IPC分类号： G01N33/553

CPC分类号： G01N33/54326

摘要： According to an aspect of the invention, there is provided a method for detecting a target compound including: injecting a test liquid containing a target compound into a colloid solution of magnetic nano-particles having an average particle diameter of 50 nm or less to allow the target compound to bind to the magnetic nano-particles, thereby forming bound magnetic nano-particles having a diameter of 100 nm or more; and bringing a dispersion liquid containing the bound magnetic nano-particles in proximity to a magnetic sensor including at least a magnetoresistive (MR) element and a permanent magnet while measuring the change in magnetic resistance to selectively detect the bound magnetic nano-particles, thereby indirectly detecting the target compound.

摘要翻译： 根据本发明的一个方面，提供了一种检测目标化合物的方法，包括：将含有目标化合物的测试液体注入到平均粒径为50nm或更小的磁性纳米颗粒的胶体溶液中， 目标化合物结合磁性纳米粒子，从而形成直径为100nm以上的结合磁性纳米粒子; 并将包含结合的磁性纳米颗粒的分散液在包括至少磁阻（MR）元件和永磁体的磁性传感器附近，同时测量磁阻的变化，从而间接地选择性地检测结合的磁性纳米颗粒 检测目标化合物。

公开(公告)号：US20070224705A1

公开(公告)日：2007-09-27

申请号：US11723634

申请日：2007-03-21

申请人： Hiroyuki Hirai ,  Masayoshi Kojima ,  Isao Tsuyuki

发明人： Hiroyuki Hirai ,  Masayoshi Kojima ,  Isao Tsuyuki

IPC分类号： G01N33/553

CPC分类号： G01N33/54326

摘要： According to an aspect of the invention, there is provided a method for detecting a target compound including: injecting a test liquid containing a target compound into a colloid solution of magnetic nano-particles having an average particle diameter of 50 nm or less to allow the target compound to bind to the magnetic nano-particles, thereby forming bound magnetic nano-particles having a diameter of 100 nm or more; and bringing a dispersion liquid containing the bound magnetic nano-particles in proximity to a magnetic sensor including at least a magnetoresistive (MR) element and a permanent magnet while measuring the change in magnetic resistance to selectively detect the bound magnetic nano-particles, thereby indirectly detecting the target compound.

摘要翻译： 根据本发明的一个方面，提供了一种检测目标化合物的方法，包括：将含有目标化合物的测试液体注入到平均粒径为50nm或更小的磁性纳米颗粒的胶体溶液中， 目标化合物结合磁性纳米粒子，从而形成直径为100nm以上的结合磁性纳米粒子; 并将包含结合的磁性纳米颗粒的分散液在包括至少磁阻（MR）元件和永磁体的磁性传感器附近，同时测量磁阻的变化，从而间接地选择性地检测结合的磁性纳米颗粒 检测目标化合物。