公开(公告)号：US20210087610A9

公开(公告)日：2021-03-25

申请号：US16081083

申请日：2018-01-12

申请人： Massachusetts Institute of Technology

发明人： Todd Michael Gierahn ,  Ang Tu ,  J. Christopher Love

IPC分类号： C12Q1/6809 ,  C12Q1/6881 ,  C40B30/04 ,  C40B20/04 ,  C12P19/34

摘要： The invention includes methods for enriching for T cell receptor- or B cell receptor-encoding transcripts from a single cell RNA sequencing library, (ii) sequencing T cell receptor- or B cell receptor-encoding transcripts from a single cell RNA sequencing library, and (iii) targeted tagmentation of T cell receptor- or B cell receptor-encoding transcripts.

公开(公告)号：US20190218607A1

公开(公告)日：2019-07-18

申请号：US16213551

申请日：2018-12-07

申请人： Massachusetts Institute of Technology

发明人： J. Christopher Love ,  Todd Michael Gierahn ,  Alexander K. Shalek ,  Marc Havens Wadsworth ,  Travis K. Hughes

IPC分类号： C12Q1/6874 ,  C12N15/10 ,  B01L3/00

CPC分类号： C12Q1/6874 ,  B01L3/5085 ,  B01L3/50855 ,  B01L2200/0647 ,  B01L2300/0681 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0851 ,  C12N15/1068 ,  C12N15/1093 ,  C12N15/1096 ,  C12Q1/6806 ,  G03F7/2014 ,  C12Q2525/15 ,  C12Q2525/155 ,  C12Q2525/191 ,  C12Q2533/101 ,  C12Q2563/179 ,  C12Q2525/173

摘要： This disclosure describes improvements to both hardware and enzymatic reactions used in single cell analyses such as but not limited to Seq-well that enable significant increases in the yield of transcripts per cell, improved portability and ease of use, increased scalability of the assay, and linkage of transcript information to other measurements made in the picowell arrays.

公开(公告)号：US20240200134A1

公开(公告)日：2024-06-20

申请号：US18448121

申请日：2023-08-10

申请人： Massachusetts Institute of Technology

发明人： J. Christopher Love ,  Todd Michael Gierahn ,  Alexander K. Shalek ,  Marc Havens Wadsworth ,  Travis K. Hughes

IPC分类号： C12Q1/6874 ,  B01L3/00 ,  C12N15/10 ,  C12Q1/6806 ,  G03F7/20

CPC分类号： C12Q1/6874 ,  B01L3/5085 ,  C12N15/1068 ,  C12N15/1093 ,  C12N15/1096 ,  C12Q1/6806 ,  G03F7/2014 ,  B01L2200/0647 ,  B01L2300/0681 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0851

摘要： This disclosure describes improvements to both hardware and enzymatic reactions used in single cell analyses such as but not limited to Seq-well that enable significant increases in the yield of transcripts per cell, improved portability and case of use, increased scalability of the assay, and linkage of transcript information to other measurements made in the picowell arrays.

公开(公告)号：US11767557B2

公开(公告)日：2023-09-26

申请号：US16213551

申请日：2018-12-07

申请人： Massachusetts Institute of Technology

发明人： J. Christopher Love ,  Todd Michael Gierahn ,  Alexander K. Shalek ,  Marc Havens Wadsworth ,  Travis K. Hughes

IPC分类号： C12Q1/6874 ,  C12Q1/6806 ,  C12N15/10 ,  G03F7/20 ,  B01L3/00

CPC分类号： C12Q1/6874 ,  B01L3/5085 ,  C12N15/1068 ,  C12N15/1093 ,  C12N15/1096 ,  C12Q1/6806 ,  G03F7/2014 ,  B01L2200/0647 ,  B01L2300/0681 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0851 ,  C12Q1/6806 ,  C12Q2525/15 ,  C12Q2525/155 ,  C12Q2525/191 ,  C12N15/1093 ,  C12Q2533/101 ,  C12Q2563/179 ,  C12N15/1096 ,  C12Q2525/173 ,  C12Q2533/101 ,  C12Q2563/179

摘要： Modifications to both hardware and enzymatic reactions used in single cell analyses such as but not limited to Seq-well that enable significant increases in the yield of transcripts per cell, portability and ease of use, increased scalability of the assay, and linkage of transcript information to other measurements made in the picowell arrays are disclosed.

公开(公告)号：US20200095625A1

公开(公告)日：2020-03-26

申请号：US16081083

申请日：2018-01-12

申请人： Massachusetts Institute of Technology

发明人： Todd Michael Gierahn ,  Ang Tu ,  J. Christopher Love

IPC分类号： C12Q1/6809 ,  C12Q1/6881 ,  C40B30/04 ,  C40B20/04 ,  C12P19/34

摘要： The invention includes methods for enriching for T cell receptor- or B cell receptor-encoding transcripts from a single cell RNA sequencing library, (ii) sequencing T cell receptor- or B cell receptor-encoding transcripts from a single cell RNA sequencing library, and (iii) targeted tagmentation of T cell receptor- or B cell receptor-encoding transcripts.

公开(公告)号：US20140307931A1

公开(公告)日：2014-10-16

申请号：US14253230

申请日：2014-04-15

申请人： Massachusetts Institute of Technology

发明人： Todd Michael Gierahn ,  Denis Loginov ,  John Christopher Love

IPC分类号： G06T7/00

CPC分类号： G06T7/11 ,  G06T2207/10056 ,  G06T2207/20068 ,  G06T2207/30072

摘要： A system for providing fully automated image segmentation and quality control of a protein array with no human interaction is presented. The system includes a memory and a processor configured by the memory. The processor is configured to receive an image file of the protein array, to geometrically distinguish a first block within the image, register a nominal location of the first block to the image by assigning a geometric code identifying the first block, draw a plurality of grid line on the array to delineate the first block from a second block, iteratively cluster a sub image for each feature in the delineated blocks to define a foreground area and a background area, identify pixels containing an artifact for each feature, and extract and return the signal intensity, variance, and quality of background and foreground pixels in each feature.

摘要翻译： 提出了一种能够提供全自动图像分割和蛋白质阵列质量控制的系统，无需人为的相互作用。 该系统包括由存储器配置的存储器和处理器。 处理器被配置为接收蛋白质阵列的图像文件，以几何地区分图像内的第一块，通过分配识别第一块的几何代码将第一块的标称位置注册到图像，绘制多个栅格 在第二个数据块上划分第一个数据块，对所划分的数据块中的每一个特征迭代地聚集一个子图像，以定义一个前景区域和一个背景区域，识别包含每个特征的工件的像素，并提取并返回 每个特征中的背景和前景像素的信号强度，方差和质量。