公开(公告)号：US20070259355A1

公开(公告)日：2007-11-08

申请号：US11547948

申请日：2005-04-08

申请人： Markus Luy ,  Matthias Rusing

发明人： Markus Luy ,  Matthias Rusing

IPC分类号： C07H21/04 ,  C07K14/00 ,  C12N1/20 ,  C12Q1/68

CPC分类号： C12Q1/689

摘要： The invention relates to a method for the rapid and simple identification of PUFA PKS in microorganisms. Said method is characterized by the fact that DNA sections representing PKS that produce specifically for PUFA (polyunsaturated fatty acids) are reproduced in vitro. The PUFA PKS-specific amino acid sequence LGIDSIKRVEIL makes it possible to derive oligonucleotides which are used for the efficient PCR screening for PUFA PKS genes or PUFA-producing microorganisms. The inventive method is particularly suitable for the high throughput screening of microorganisms for PUFA PKS genes.

摘要翻译： 本发明涉及用于快速和简单地鉴定微生物中PUFA PKS的方法。 所述方法的特征在于代表PKS产生的专门用于PUFA（多不饱和脂肪酸）的DNA片段在体外再现。 PUFA PKS特异性氨基酸序列LGIDSIKRVEIL可以获得用于有效PCR筛选PUFA PKS基因或产生PUFA的微生物的寡核苷酸。 本发明的方法特别适合用于PUFA PKS基因的微生物的高通量筛选。

公开(公告)号：US20070054384A1

公开(公告)日：2007-03-08

申请号：US10578968

申请日：2004-11-10

申请人： Matthias Rusing ,  Markus Luy

发明人： Matthias Rusing ,  Markus Luy

IPC分类号： C12P7/64 ,  C12N1/21 ,  A23K1/00

CPC分类号： C12P7/6427 ,  A23K10/37 ,  A23L33/12 ,  A23V2002/00 ,  C12N1/12 ,  C12P7/6472

摘要： The invention relates to an optimized method for cultivating microorganisms of the genus Thraustochytriales, according to which the microorganisms are cultivated in a low salt medium without adding sodium salts and chloride salts, the total salt content being less than 3.5 g/L (corresponding to less than 10 percent of sea water salt content), whereupon the PUFAs are isolated from the microorganisms and/or the medium. The invention especially relates to novel optimized media having a substantially reduced total salt content, above all a particularly reduced NaCl content. The production of PUFAs can be substantially improved and significantly simplified by using a novel combination of different salts as a media composition in which the overall weight ratios of ions do not exceed 1.75 g/L. Furthermore, said medium preferably contains no added sodium salt and chloride salt at all, which helps prevent environmental damages caused by wastewaters containing salt.

摘要翻译： 本发明涉及一种用于培养破囊壶菌属微生物的优化方法，根据该方法，微生物在低盐培养基中培养而不添加钠盐和氯化物盐，总盐含量小于3.5g / L（相当于少于 超过10％的海水盐含量），由此从微生物和/或介质中分离PUFA。 本发明特别涉及具有显着降低的总盐含量，特别是特别降低的NaCl含量的新型优化的介质。 通过使用不同盐的新型组合作为其中离子的总重量比不超过1.75g / L的介质组合物，可以显着改善和显着地简化PUFA的生产。 此外，所述介质优选根本不含有添加的钠盐和氯化物盐，这有助于防止由含有盐的废水引起的环境损害。

公开(公告)号：US20090093033A1

公开(公告)日：2009-04-09

申请号：US11547921

申请日：2005-04-08

申请人： Markus Luy ,  Matthias Rusing ,  Thomas Kiy

发明人： Markus Luy ,  Matthias Rusing ,  Thomas Kiy

IPC分类号： C12P7/64 ,  C12N9/00 ,  C12N15/11 ,  C11B1/00 ,  C12N15/00 ,  C12N1/21

CPC分类号： C12P7/6472 ,  C12N15/52

摘要： The invention relates to genes which are coded for sequences specific to polyketide synthases (PKS). The thus synthesized PKS is characterized by the enzymatic capacity thereof to produce PUFAs (polyunsaturated fatty acids). The invention also relates to the identification of the corresponding DNA-sequences, in addition to the use of said nucleotide sequences for the production of recombined and/or transgenic organisms.

摘要翻译： 本发明涉及编码针对聚酮化合物合酶（PKS）特异性序列的基因。 由此合成的PKS的特征在于其产生PUFA（多不饱和脂肪酸）的酶能力。 除了使用所述核苷酸序列产生重组和/或转基因生物外，本发明还涉及鉴定相应的DNA序列。

公开(公告)号：US20070141686A1

公开(公告)日：2007-06-21

申请号：US10578965

申请日：2004-11-10

申请人： Markus Luy ,  Matthias Rusing

发明人： Markus Luy ,  Matthias Rusing

IPC分类号： C12P7/62 ,  C12N1/21

CPC分类号： C12P7/6427 ,  C12N1/14 ,  C12P7/6472

摘要： The invention relates to an optimized method for the production of PUFAs by cultivating microorganisms belonging to the group of Stramenopiles in a fermentation medium that is pH-stabilized using calcium carbonate and comprises 3-15 g/L CaCO3, whereupon the PUFAs are isolated from the microorganisms and/or the medium. The invention particularly relates to novel optimized media having a different CaCO3 content. By using adequate quantities of CaCO3, the process can be significantly simplified during fermentation while greater quantities of DHA can be obtained at an increased oil content in the biomass. They allow microorganisms belonging to the Stramenopiles to be fermented without controlling the pH, thereby substantially improving and significantly simplifying PUFA production.

摘要翻译： 本发明涉及通过在使用碳酸钙进行pH稳定化的发酵培养基中培养属于Stramenopiles组的微生物并且包含3-15g / L CaCO 3来生产PUFA的优化方法，由此将PUFA从 微生物和/或培养基。 本发明特别涉及具有不同CaCO3含量的新型优化介质。 通过使用足够量的CaCO 3，可以在发酵期间显着简化该过程，同时在生物质中增加的油含量可获得更多量的DHA。 它们允许属于Stramenopiles的微生物发酵而不控制pH值，从而显着改善和显着简化PUFA生产。

公开(公告)号：US07135623B1

公开(公告)日：2006-11-14

申请号：US10070666

申请日：2000-09-08

申请人： Matthias Rusing ,  Thomas Kiy ,  Annette Dominitzki

发明人： Matthias Rusing ,  Thomas Kiy ,  Annette Dominitzki

IPC分类号： A01H5/00 ,  C12N15/82 ,  A01K67/00

CPC分类号： C12N9/0083 ,  C12P7/6427 ,  C12P7/6472

摘要： The invention relates to nucleic acid(s) which is/are obtained from tetrahymena and which code(s) for a ciliate-specific delta-6-desaturase that is involved in the biosynthesis of commercially valuable, multiply unsaturated fatty acids (so-called PUFA) polyunsaturated fatty acids). The inventive nucleotide sequence and the polypeptide sequence that can be obtained therefrom exhibit a surprisingly low sequence identity compared to other known natural desaturases. The invention also relates to the use of the nucleic acid(s) for overexpression in ciliates, preferably tetrahymena, in particular, Tetrahymena thermophila, with the aim of increasing the production of delta-6 unsaturated fatty acids, especially GLA.

摘要翻译： 本发明涉及从四膜虫获得的核酸，以及参与商业上有价值的多重不饱和脂肪酸的生物合成（所谓的“多不饱和脂肪酸”）的细胞特异性δ-6-去饱和酶的编码 PUFA）多不饱和脂肪酸）。 与其它已知的天然去饱和酶相比，本发明的可从其获得的核苷酸序列和多肽序列表现出惊人的低序列同一性。 本发明还涉及核酸在纤毛虫，优选四膜虫，特别是嗜热四膜虫中的过度表达的用途，目的是增加δ-6不饱和脂肪酸，特别是GLA的产生。

公开(公告)号：US20070010480A1

公开(公告)日：2007-01-11

申请号：US10558320

申请日：2004-05-26

申请人： Matthias Rusing ,  Stephan Hausmanns

发明人： Matthias Rusing ,  Stephan Hausmanns

IPC分类号： A61K31/718 ,  A61K31/716 ,  A61K31/202 ,  A61K31/715

CPC分类号： A23G3/36 ,  A23G3/368 ,  A23G3/42 ,  A23L2/39 ,  A23L2/52 ,  A23L33/12 ,  A23L33/22 ,  A23L33/30 ,  A23L33/40 ,  A23V2002/00 ,  A23V2250/1868 ,  A23V2250/5046 ,  A23V2250/5062 ,  A23V2250/5424 ,  A23V2250/70 ,  A23V2250/704 ,  A23V2250/712 ,  A23V2250/156

摘要： The invention relates to dietetic foods in the context of weight control or weight reduction, comprising at least one n3 fatty acid (omega-3) in combination with dietary fibers. Advantageously, the foods further comprise a balanced ratio of carbohydrates, protein and fat. Preferably, the inventive foods comprise a balanced ratio of fatty acids, in particular relating to the ratio n3 to n6 fatty acids (approximately 1:1 to 1:10). The inventive food combinations serve the purpose of a sufficient supply of essential fatty acids, in particular long-chain omega-3 fatty acids, in particular docosahexaenoic acid (DHA), in the context of a targeted weight reduction. The inventive food combinations are of particular advantage in the case of restricted fat supply, e.g. in the context of a reduced-fat diet with the purpose of weight reduction or weight control. In addition, the invention relates to a method for producing such dietetic foods, and also to their use.

摘要翻译： 本发明涉及在体重控制或体重减轻的背景下的饮食食物，其包含至少一种与膳食纤维组合的n3脂肪酸（ω-3）。 有利地，食物还包括碳水化合物，蛋白质和脂肪的平衡比例。 优选地，本发明的食品包括脂肪酸的平衡比例，特别是与n 3至n 6脂肪酸的比例（约1:1至1:10）。 本发明的食物组合用于在目标重量减少的情况下足够供应必需脂肪酸，特别是长链ω-3脂肪酸，特别是二十二碳六烯酸（DHA）的目的。 在限制性脂肪供应的情况下，本发明的食物组合是特别有利的。 在减肥或减肥的目的下，减肥饮食。 此外，本发明涉及一种生产这种饮食食品的方法，也涉及它们的用途。