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公开(公告)号:US09074204B2
公开(公告)日:2015-07-07
申请号:US13476911
申请日:2012-05-21
申请人: Megan Anderson , Peilin Chen , Brian Fowler , Robert C. Jones , Fiona Kaper , Ronald Lebofsky , Andrew May
发明人: Megan Anderson , Peilin Chen , Brian Fowler , Robert C. Jones , Fiona Kaper , Ronald Lebofsky , Andrew May
CPC分类号: C12Q1/6855 , C12N15/10 , C12N15/1065 , C12N15/65 , C12N15/66 , C12Q1/686 , C12Q1/6874
摘要: Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.
摘要翻译: 本文所描述的方法可用于掺入一个或多个衔接子和/或核苷酸标签和/或条形码核苷酸序列,一个或多个靶核苷酸序列。 在具体实施方案中,产生具有适配器(例如适合用于高通量DNA测序)的核酸片段。 在其它实施方案中,关于反应混合物的信息被编码成反应产物。 本文还描述了可用于扩增一种或多种靶核酸以准备应用如双向核酸测序的方法和试剂盒。 在具体实施方案中,本发明的方法还需要进行双向DNA测序。 本文还描述了通过引物延伸来编码和检测和/或定量等位基因的方法。
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公开(公告)号:US20130005585A1
公开(公告)日:2013-01-03
申请号:US13476911
申请日:2012-05-21
申请人: Megan Anderson , Peilin Chen , Brian Fowler , Robert C. Jones , Fiona Kaper , Ronald Lebofsky , Andrew May
发明人: Megan Anderson , Peilin Chen , Brian Fowler , Robert C. Jones , Fiona Kaper , Ronald Lebofsky , Andrew May
CPC分类号: C12Q1/6855 , C12N15/10 , C12N15/1065 , C12N15/65 , C12N15/66 , C12Q1/686 , C12Q1/6874
摘要: Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.
摘要翻译: 本文所描述的方法可用于掺入一个或多个衔接子和/或核苷酸标签和/或条形码核苷酸序列,一个或多个靶核苷酸序列。 在具体实施方案中,产生具有适配器(例如适合用于高通量DNA测序)的核酸片段。 在其它实施方案中,关于反应混合物的信息被编码成反应产物。 本文还描述了可用于扩增一种或多种靶核酸以准备应用如双向核酸测序的方法和试剂盒。 在具体实施方案中,本发明的方法还需要进行双向DNA测序。 本文还描述了通过引物延伸来编码和检测和/或定量等位基因的方法。
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3.
公开(公告)号:US08691509B2
公开(公告)日:2014-04-08
申请号:US12753703
申请日:2010-04-02
申请人: Andrew May , Peilin Chen , Jun Wang , Fiona Kaper , Megan Anderson
发明人: Andrew May , Peilin Chen , Jun Wang , Fiona Kaper , Megan Anderson
CPC分类号: C12Q1/6806 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2400/0487 , B01L2400/0655 , C12Q1/686 , C12Q2525/155 , C12Q2525/161 , C12Q2527/143 , C12Q2535/122 , C12Q2549/119 , C12Q2563/179 , C12Q2565/629
摘要: In certain embodiments, the invention provides amplification methods in which nucleotide tag(s) and a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.
摘要翻译: 在某些实施方案中,本发明提供其中将核苷酸标签和条形码核苷酸序列添加到靶核苷酸序列的扩增方法。 在其它实施例中,本发明提供一种包括多个第一输入线和多个第二输入线的微流体装置。 微流体装置还包括多组第一腔室和多组第二腔室。 每组第一腔室与多个第一输入管线中的一个流体连通。 每组第二腔室与多个第二输入管线中的一个流体连通。 微流体装置还包括与多个第二输入线的第一部分流体连通的多个第一泵元件和与多个第二输入线的第二部分流体连通的多个第二泵元件。
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4.
公开(公告)号:US20100273219A1
公开(公告)日:2010-10-28
申请号:US12753703
申请日:2010-04-02
申请人: Andrew May , Peilin Chen , Jun Wang , Fiona Kaper , Megan Anderson
发明人: Andrew May , Peilin Chen , Jun Wang , Fiona Kaper , Megan Anderson
CPC分类号: C12Q1/6806 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2400/0487 , B01L2400/0655 , C12Q1/686 , C12Q2525/155 , C12Q2525/161 , C12Q2527/143 , C12Q2535/122 , C12Q2549/119 , C12Q2563/179 , C12Q2565/629
摘要: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.
摘要翻译: 在某些实施方案中,本发明提供了其中将核苷酸标签和任选的条形码核苷酸序列加入靶核苷酸序列的扩增方法。 在其它实施例中,本发明提供一种包括多个第一输入线和多个第二输入线的微流体装置。 微流体装置还包括多组第一腔室和多组第二腔室。 每组第一腔室与多个第一输入管线中的一个流体连通。 每组第二腔室与多个第二输入管线中的一个流体连通。 微流体装置还包括与多个第二输入线的第一部分流体连通的多个第一泵元件和与多个第二输入线的第二部分流体连通的多个第二泵元件。
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