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    Enzyme triggered redox altering chemical elimination (E-trace) immunoassay
    9.
    发明授权
    Enzyme triggered redox altering chemical elimination (E-trace) immunoassay 有权
    酶触发氧化还原改变化学清除（E-trace）免疫测定

    公开(公告)号：US08530170B2

    公开(公告)日：2013-09-10

    申请号：US12853204

    申请日：2010-08-09

    申请人： Paul A. Bertin

    发明人： Paul A. Bertin

    IPC分类号： G01N33/53

    CPC分类号： G01N27/26 ,  G01N33/5438 ,  G01N2610/00

    摘要： The present invention is directed to electronic methods of detecting target analytes such that upon binding of the target analyte a shift in electrochemical potential is seen. This assay relies on the use of an electroactive moiety (“EAM”) that is attached to an electrode and comprises a self-immolative moiety, whose presence gives the EAM a first E0, and whose absence, upon irreversible cleavage gives the EAM a second E0. This difference is detected, and if such change occurs, it is an indication of the presence of a target analyte.

    摘要翻译： 本发明涉及检测目标分析物的电子方法，使得在目标分析物结合时可以看到电化学势的偏移。 该测定依赖于使用连接到电极上的电活性部分（“EAM”），并且包含自身残留部分，其存在给予EAM为第一E0，并且当不可逆切割时，其不存在使得EAM为第二 E0。 检测到这种差异，如果发生这种变化，则表明存在目标分析物。

    Enzyme Triggered Redox Altering Chemical Elimination (E-Trace) Immunoassay
    10.
    发明申请
    Enzyme Triggered Redox Altering Chemical Elimination (E-Trace) Immunoassay 有权
    酶触发氧化还原改变化学消除（E-痕量）免疫测定

    公开(公告)号：US20120181186A1

    公开(公告)日：2012-07-19

    申请号：US13354200

    申请日：2012-01-19

    申请人： Paul A. Bertin ,  Michael J. Ahrens

    发明人： Paul A. Bertin ,  Michael J. Ahrens

    IPC分类号： C12Q1/28 ,  C12Q1/48

    CPC分类号： G01N33/54306 ,  C12Q1/005 ,  G01N31/22

    摘要： Described are methods for detecting a target analyte in a test sample by providing a solid support comprising an electrode comprising: (i) a self-assembled monolayer (SAM), (ii) a covalently attached electroactive active moiety (EAM) comprising a transition metal complex comprising a self-immolative moiety and a peroxide sensitive moiety (PSM), wherein said EAM has a first E0; then contacting the target analyte and said solid support under conditions wherein said target analyte reacts with a peroxide generating enzyme to generate peroxide and said self-immolative moiety is removed such that said EAM has a second E0 test sample; and then detecting said second E0 as an indication of the presence of said target analyte. Also provided are compositions for use in the preceding methods.

    摘要翻译： 描述了通过提供包括电极的固体支持物来检测测试样品中的目标分析物的方法，所述固体支持物包括：（i）自组装单层（SAM），（ii）共价连接的电活性活性部分（EAM），其包含过渡金属 复合物，其包含自身残留部分和过氧化物敏感部分（PSM），其中所述EAM具有第一E0; 然后在其中所述目标分析物与产生过氧化物的酶反应产生过氧化物的条件下接触目标分析物和所述固体支持物，并且去除所述自消除部分，使得所述EAM具有第二种E0测试样品; 然后检测所述第二E0作为所述目标分析物的存在的指示。 还提供了用于前述方法的组合物。