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    IRREVERSIBLE ELECTROPORATION USING TISSUE VASCULATURE TO TREAT ABERRANT CELL MASSES OR CREATE TISSUE SCAFFOLDS
    1.
    发明申请
    IRREVERSIBLE ELECTROPORATION USING TISSUE VASCULATURE TO TREAT ABERRANT CELL MASSES OR CREATE TISSUE SCAFFOLDS 有权
    不可逆电气使用组织血管来治疗细胞质量或产生组织病变

    公开(公告)号：US20130253415A1

    公开(公告)日：2013-09-26

    申请号：US13989175

    申请日：2011-11-23

    申请人： Michael B. Sano ,  Rafael V. Davalos ,  John L. Robertson ,  Paulo A. Garcia ,  Robert E. Neal

    发明人： Michael B. Sano ,  Rafael V. Davalos ,  John L. Robertson ,  Paulo A. Garcia ,  Robert E. Neal

    IPC分类号： A61B18/14 ,  A61M5/00

    CPC分类号： A61B18/14 ,  A61B18/1492 ,  A61B2018/00613 ,  A61B2018/1472 ,  A61B2218/002 ,  A61K48/0075 ,  A61M5/00 ,  A61N1/327

    摘要： The present invention relates to the field of medical treatment of diseases and disorders, as well as the field of biomedical engineering. Embodiments of the invention relate to the delivery of Irreversible Electroporation (IRE) through the vasculature of organs to treat tumors embedded deep within the tissue or organ, or to decellularize organs to produce a scaffold from existing animal tissue with the existing vasculature intact. In particular, methods of administering non-thermal irreversible electroporation (IRE) in vivo are provided for the treatment of tumors located in vascularized tissues and organs. Embodiments of the invention further provide scaffolds and tissues from natural sources created using IRE ex vivo to remove cellular debris, maximize recellularization potential, and minimize foreign body immune response. The engineered tissues can be used in methods of treating subjects, such as those in need of tissue replacement or augmentation.

    摘要翻译： 本发明涉及疾病和病症的医疗领域以及生物医学工程领域。 本发明的实施方案涉及通过器官脉管系统递送不可逆电穿孔（IRE）以治疗深入组织或器官内的肿瘤，或者使现有动物组织脱细胞从而使现有的脉管系统完整地产生支架。 特别地，提供了在体内施用非热不可逆电穿孔（IRE）的方法用于治疗位于血管化组织和器官中的肿瘤。 本发明的实施方案进一步提供了使用IRE离体产生的来自天然来源的支架和组织，以去除细胞碎片，最大限度地获得细胞结合潜能，并使异物免疫应答最小化。 工程组织可用于治疗受试者的方法，例如需要组织置换或增生的方法。

    THREE-DIMENSIONAL BIOPRINTING OF BIOSYNTHETIC CELLULOSE (BC) IMPLANTS AND SCAFFOLDS FOR TISSUE ENGINEERING
    8.
    发明申请
    THREE-DIMENSIONAL BIOPRINTING OF BIOSYNTHETIC CELLULOSE (BC) IMPLANTS AND SCAFFOLDS FOR TISSUE ENGINEERING 有权
    生物塑料纤维素（BC）植物和组织的组织工程三维生物学

    公开(公告)号：US20120190078A1

    公开(公告)日：2012-07-26

    申请号：US13498657

    申请日：2010-09-28

    申请人： Paul Gatenholm ,  Henrik Backdahl ,  Theodore Jon Tzavaras ,  Rafael V. Davalos ,  Michael B. Sano

    发明人： Paul Gatenholm ,  Henrik Backdahl ,  Theodore Jon Tzavaras ,  Rafael V. Davalos ,  Michael B. Sano

    IPC分类号： C12P19/04 ,  C12N5/02 ,  C12N11/12

    CPC分类号： C08B1/00 ,  A61L27/20 ,  A61L27/38 ,  A61L27/56 ,  A61L2400/12 ,  C12N5/0062 ,  C12N11/12 ,  C12N2533/78 ,  C12P19/04 ,  C08L1/02

    摘要： A novel BC fermentation technique for controlling 3D shape, thickness and architecture of the entangled cellulose nano-fibril network is presented. The resultant nano-cellulose based structures are useful as biomedical implants and devices, are useful for tissue engineering and regenerative medicine, and for health care products. More particularly, embodiments of the present invention relate to systems and methods for the production and control of 3-D architecture and morphology of nano-cellulose biomaterials produced by bacteria using any biofabrication process, including the novel 3-D Bioprinting processes disclosed. Representative processes according to the invention involve control of the rate of production of biomaterial by bacteria achieved by meticulous control of the addition of fermentation media using a microfluidic system. In exemplary embodiments, the bacteria gradually grew up along the printed alginate structure that had been placed into the culture, incorporating it. After culture, the printed alginate structure was successfully removed revealing porosity where the alginate had been placed. Porosity and interconnectivity of pores in the resultant 3-D architecture can be achieved by porogen introduction using, e.g., ink-jet printer technology.

    摘要翻译： 提出了一种用于控制缠结纤维素纳米纤维网络的3D形状，厚度和结构的新型BC发酵技术。 所得的纳米纤维素基结构可用作生物医学植入物和装置，可用于组织工程和再生医学以及保健产品。 更具体地说，本发明的实施方案涉及使用任何生物制造方法生产和控制由细菌生产的纳米纤维素生物材料的三维结构和形态的系统和方法，包括所公开的新型3-D生物印迹方法。 根据本发明的代表性方法涉及通过细微控制使用微流体系统添加发酵培养基来控制细菌生物材料的生产速率。 在示例性实施方案中，细菌沿已经放入培养物中的印刷的藻酸盐结构逐渐长大，并入其中。 培养后，成功去除了印刷的藻酸盐结构，显示了藻酸盐放置的孔隙度。 所得3-D结构中孔隙的孔隙率和互连性可以通过使用例如喷墨打印机技术的致孔剂引入来实现。

    Three-dimensional bioprinting of biosynthetic cellulose (BC) implants and scaffolds for tissue engineering
    9.
    发明授权
    Three-dimensional bioprinting of biosynthetic cellulose (BC) implants and scaffolds for tissue engineering 有权
    生物合成纤维素（BC）植入物和组织工程支架的三维生物印迹

    公开(公告)号：US08691974B2

    公开(公告)日：2014-04-08

    申请号：US13498657

    申请日：2010-09-28

    申请人： Paul Gatenholm ,  Henrik Backdahl ,  Theodore Jon Tzavaras ,  Rafael V. Davalos ,  Michael B. Sano

    发明人： Paul Gatenholm ,  Henrik Backdahl ,  Theodore Jon Tzavaras ,  Rafael V. Davalos ,  Michael B. Sano

    IPC分类号： C08B1/00 ,  A61K31/717 ,  C12P1/04 ,  C12N11/12

    CPC分类号： C08B1/00 ,  A61L27/20 ,  A61L27/38 ,  A61L27/56 ,  A61L2400/12 ,  C12N5/0062 ,  C12N11/12 ,  C12N2533/78 ,  C12P19/04 ,  C08L1/02

    摘要： A novel BC fermentation technique for controlling 3D shape, thickness and architecture of the entangled cellulose nano-fibril network is presented. The resultant nano-cellulose based structures are useful as biomedical implants and devices, are useful for tissue engineering and regenerative medicine, and for health care products. More particularly, embodiments of the present invention relate to systems and methods for the production and control of 3-D architecture and morphology of nano-cellulose biomaterials produced by bacteria using any biofabrication process, including the novel 3-D Bioprinting processes disclosed. Representative processes according to the invention involve control of the rate of production of biomaterial by bacteria achieved by meticulous control of the addition of fermentation media using a microfluidic system. In exemplary embodiments, the bacteria gradually grew up along the printed alginate structure that had been placed into the culture, incorporating it. After culture, the printed alginate structure was successfully removed revealing porosity where the alginate had been placed. Porosity and interconnectivity of pores in the resultant 3-D architecture can be achieved by porogen introduction using, e.g., ink-jet printer technology.

    摘要翻译： 提出了一种用于控制缠结纤维素纳米纤维网络的3D形状，厚度和结构的新型BC发酵技术。 所得的纳米纤维素基结构可用作生物医学植入物和装置，可用于组织工程和再生医学以及保健产品。 更具体地说，本发明的实施方案涉及使用任何生物制造方法生产和控制由细菌生产的纳米纤维素生物材料的三维结构和形态的系统和方法，包括所公开的新型3-D生物印迹方法。 根据本发明的代表性方法涉及通过细微控制使用微流体系统添加发酵培养基来控制细菌生物材料的生产速率。 在示例性实施方案中，细菌沿已经放入培养物中的印刷的藻酸盐结构逐渐长大，并入其中。 培养后，成功去除了印刷的藻酸盐结构，显示了藻酸盐放置的孔隙度。 所得3-D结构中孔隙的孔隙率和互连性可以通过使用例如喷墨打印机技术的致孔剂引入来实现。

    DIELECTROPHORESIS DEVICES AND METHODS THEREFOR
    10.
    发明申请
    DIELECTROPHORESIS DEVICES AND METHODS THEREFOR 审中-公开
    电介质器件及其方法

    公开(公告)号：US20120085649A1

    公开(公告)日：2012-04-12

    申请号：US13269286

    申请日：2011-10-07

    申请人： Michael B. Sano ,  John L. Caldwell ,  Rafael V. Davalos

    发明人： Michael B. Sano ,  John L. Caldwell ,  Rafael V. Davalos

    IPC分类号： G01N27/453

    CPC分类号： B03C5/005 ,  B03C5/026 ,  B03C2201/26

    摘要： Devices and methods for performing dielectrophoresis are described. The devices contain a sample channel which is separated by physical barriers from electrode channels which receive electrodes. The devices and methods may be used for the separation and analysis of particles in solution, including the separation and isolation of cells of a specific type. As the electrodes do not make contact with the sample, electrode fouling is avoided and sample integrity is better maintained.

    摘要翻译： 描述了用于进行介电电泳的装置和方法。 这些器件包含一个样品通道，该通道由接收电极的电极通道的物理屏障分开。 该装置和方法可用于分离和分析溶液中的颗粒，包括分离和分离特定类型的细胞。 由于电极不与样品接触，避免了电极结垢，更好地保持了样品的完整性。