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    Method for generating libaries of antibody genes comprising amplification of diverse antibody DNAs and methods for using these libraries for the production of diverse antigen combining molecules
    3.
    发明授权
    Method for generating libaries of antibody genes comprising amplification of diverse antibody DNAs and methods for using these libraries for the production of diverse antigen combining molecules 失效
    产生包含多种抗体DNA扩增的抗体基因库的方法以及使用这些文库生产多种抗原结合分子的方法

    公开(公告)号：US5780225A

    公开(公告)日：1998-07-14

    申请号：US315269

    申请日：1994-09-29

    申请人： Michael H. Wigler ,  Joseph A. Sorge

    发明人： Michael H. Wigler ,  Joseph A. Sorge

    IPC分类号： C12N15/10 ,  C12Q1/68 ,  C12P21/00

    CPC分类号： C12Q1/6811 ,  C12N15/10 ,  C07K2319/00

    摘要： A method of producing libraries of genes encoding antigen-combining molecules or antibodies; a method of producing antigen-combining molecules which does not require an in vivo procedure; a method of obtaining antigen-combining molecules of selected specificity which does not require an in vivo procedure; vectors useful in the present method; and antigen-combining molecules produced by the method. The antigen-combining molecules are useful for the detection, quantitation, purification and neutralization of antigens, as well as for diagnostic, therapeutic and prophylactic purposes.

    摘要翻译： 一种产生编码抗原结合分子或抗体的基因文库的方法; 生产不需要体内程序的抗原结合分子的方法; 获得不需要体内过程的所选特异性的抗原结合分子的方法; 本方法有用的载体; 和通过该方法产生的抗原结合分子。 抗原结合分子可用于抗原的检测，定量，纯化和中和，以及用于诊断，治疗和预防目的。

    Method for generating libraries of antibody genes comprising amplification of diverse antibody DNAs and methods for using these libraries for the production of diverse antigen combining molecules
    4.
    发明授权
    Method for generating libraries of antibody genes comprising amplification of diverse antibody DNAs and methods for using these libraries for the production of diverse antigen combining molecules 失效
    产生抗体基因文库的方法，包括扩增多种抗体DNA，以及使用这些文库生产多种抗原结合分子的方法

    公开(公告)号：US06303313B1

    公开(公告)日：2001-10-16

    申请号：US09439732

    申请日：1999-11-12

    申请人： Michael H. Wigler ,  Joseph A. Sorge

    发明人： Michael H. Wigler ,  Joseph A. Sorge

    IPC分类号： C12Q168

    CPC分类号： C12N15/102 ,  A61K38/00 ,  C07K16/00 ,  C07K2319/00 ,  C12N15/10

    摘要： A method of producing libraries of genes encoding antigen-combining molecules or antibodies; a method of producing antigen-combining molecules which does not require an in vivo procedure; a method of obtaining antigen-combining molecules of selected specificity which does not require an in vivo procedure; vectors useful in the present method; and antigen-combining molecules produced by the method. The antigen-combining molecules are useful for the detection, quantitation, purification and neutralization of antigens, as well as for diagnostic, therapeutic and prophylactic purposes.

    摘要翻译： 一种产生编码抗原结合分子或抗体的基因文库的方法; 生产不需要体内程序的抗原结合分子的方法; 获得不需要体内过程的所选特异性的抗原结合分子的方法; 本方法有用的载体; 和通过该方法产生的抗原结合分子。 抗原结合分子可用于抗原的检测，定量，纯化和中和，以及用于诊断，治疗和预防目的。

    Methods of enriching for and identifying polymorphisms
    5.
    发明授权
    Methods of enriching for and identifying polymorphisms 有权
    富集和鉴定多态性的方法

    公开(公告)号：US08383343B2

    公开(公告)日：2013-02-26

    申请号：US10391090

    申请日：2003-03-18

    申请人： Joseph A. Sorge

    发明人： Joseph A. Sorge

    IPC分类号： C12Q1/68

    CPC分类号： C12N15/1072 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q2565/519 ,  C12Q2565/531 ,  C12Q2533/101 ,  C12Q2522/101

    摘要： The invention encompasses methods for enriching for and identifying a polymorphism within a nucleic acid sample either by separating a subset of a nucleic acid sample or by selectively replicating a subset of a nucleic acid sample such that the polymorphism is contained within a nucleic acid population with reduced complexity, and then identifying the polymorphism within the enriched nucleic acid sample. Methods also are disclosed for enriching for and identifying a polymorphism by contacting a nucleic acid sample that includes a subset of nucleic acid molecules having a sequence that binds to a sequence-specific binding activity with a molecule having a sequence-specific binding activity under conditions which permit specific binding, such that the subset of nucleic acid molecules bound to the activity is enriched for nucleic acid molecules having the sequence recognized by the sequence-specific binding activity, and detecting a polymorphism with respect to a reference sequence in the subset of nucleic acid molecules.

    摘要翻译： 本发明包括用于通过分离核酸样品的子集或通过选择性复制核酸样品的子集来使得核酸样品中的多态性富集和鉴定核酸样品中的多态性的方法，使得多态性包含在具有降低的核酸群体的核酸群体内 复杂性，然后鉴定富集的核酸样品内的多态性。 公开了通过使包含具有与序列特异性结合活性结合的序列的核酸分子的子集与具有序列特异性结合活性的分子的核酸样品接触的方法来丰富和鉴定多态性的方法， 允许特异性结合，使得与活性结合的核酸分子的子集富含具有由序列特异性结合活性识别的序列的核酸分子，并且检测核酸子集中的参考序列的多态性 分子。

    METHODS OF ENRICHING FOR AND IDENTIFYING POLYMORPHISMS
    7.
    发明申请
    METHODS OF ENRICHING FOR AND IDENTIFYING POLYMORPHISMS 审中-公开
    增强和鉴别多态性的方法

    公开(公告)号：US20100248246A1

    公开(公告)日：2010-09-30

    申请号：US12749070

    申请日：2010-03-29

    申请人： Joseph A. Sorge ,  Ronald W. Davis

    发明人： Joseph A. Sorge ,  Ronald W. Davis

    IPC分类号： C12Q1/68

    CPC分类号： C12N15/1072 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q2565/519 ,  C12Q2565/531 ,  C12Q2533/101 ,  C12Q2522/101

    摘要： The invention encompasses methods for enriching for and identifying a polymorphism within a nucleic acid sample either by separating a subset of a nucleic acid sample or by selectively replicating a subset of a nucleic acid sample such that the polymorphism is contained within a nucleic acid population with reduced complexity, and then identifying the polymorphism within the enriched nucleic acid sample. Methods also are disclosed for enriching for and identifying a polymorphism by contacting a nucleic acid sample that includes a subset of nucleic acid molecules having a sequence that binds to a sequence-specific binding activity with a molecule having a sequence-specific binding activity under conditions which permit specific binding, such that the subset of nucleic acid molecules bound to the activity is enriched for nucleic acid molecules having the sequence recognized by the sequence-specific binding activity, and detecting a polymorphism with respect to a reference sequence in the subset of nucleic acid molecules.

    摘要翻译： 本发明包括用于通过分离核酸样品的子集或通过选择性复制核酸样品的子集来使得核酸样品中的多态性富集和鉴定核酸样品中的多态性的方法，使得多态性包含在具有降低的核酸群体的核酸群体内 复杂性，然后鉴定富集的核酸样品内的多态性。 公开了通过使包含具有与序列特异性结合活性结合的序列的核酸分子的子集与具有序列特异性结合活性的分子的核酸样品接触的方法来丰富和鉴定多态性的方法， 允许特异性结合，使得与活性结合的核酸分子的子集富含具有由序列特异性结合活性识别的序列的核酸分子，并且检测核酸子集中的参考序列的多态性 分子。

    Compositions and methods utilizing DNA polymerases
    9.
    发明申请
    Compositions and methods utilizing DNA polymerases 审中-公开
    使用DNA聚合酶的组合物和方法

    公开(公告)号：US20080199935A1

    公开(公告)日：2008-08-21

    申请号：US11644729

    申请日：2006-12-22

    申请人： Joseph A. Sorge ,  Holly Hogrefe ,  Connie J. Hansen

    发明人： Joseph A. Sorge ,  Holly Hogrefe ,  Connie J. Hansen

    IPC分类号： C12N9/12

    CPC分类号： B82Y5/00 ,  B82Y10/00 ,  C12N9/1252

    摘要： The invention features a novel isolated Family B DNA polymerase, a Thermococcus polymerase JDF-3, and mutant recombinant forms thereof. Mutant polymerases of the invention are deficient in 3′ to 5′ exonuclease activity and/or exhibit reduced discrimination against non-conventional nucleotides relative to the wild-type form of the polymerase.

    摘要翻译： 本发明的特征在于新的分离的家族B DNA聚合酶，热球菌聚合酶JDF-3及其突变体重组形式。 本发明的突变型聚合酶相对于野生型形式的聚合酶，缺乏3'至5'核酸外切酶活性和/或表现出对非常规核苷酸的降低的鉴别。

    Methods for detection of a target nucleic acid by capture
    10.
    发明授权
    Methods for detection of a target nucleic acid by capture 有权
    通过捕获检测靶核酸的方法

    公开(公告)号：US07361467B2

    公开(公告)日：2008-04-22

    申请号：US11150775

    申请日：2005-06-10

    申请人： Joseph A. Sorge ,  Anne M. Whalen

    发明人： Joseph A. Sorge ,  Anne M. Whalen

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Q1/6823 ,  C12Q1/6816 ,  C12Q2521/301 ,  C12Q2531/113 ,  C12Q2521/307 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q2561/109 ,  C12Q2565/519 ,  C12Q2561/101 ,  C12Q2521/107

    摘要： The invention relates to a method of generating a signal indicative of the presence of a target nucleic acid in a sample, where the method includes forming a cleavage structure by incubating a sample comprising a target nucleic acid with a probe having a secondary structure that changes upon binding of the probe to the target nucleic acid and further comprising a binding moiety. The invention also includes the steps of cleaving the cleavage structure with a nuclease to release a nucleic acid fragment to generate a signal, wherein generation of the signal is indicative of the presence of a target nucleic acid in a sample, and detecting and/or measuring the amount of the fragment captured by binding of a binding moiety to a capture element on a solid support.The invention also relates to a method of detecting or measuring a target nucleic acid in a sample, where the method includes forming a cleavage structure by incubating a sample containing a target nucleic acid with a probe having a secondary structure that changes upon binding of the probe to a target nucleic acid and comprising a binding moiety, and cleaving the cleavage structure with a nuclease to generate a cleaved nucleic acid fragment and detecting and/or measuring the amount of the fragment captured by binding of a binding moiety to a capture element on a solid support.

    摘要翻译： 本发明涉及产生指示样品中目标核酸存在的信号的方法，其中该方法包括通过将包含靶核酸的样品与具有二级结构变化的探针一起孵育形成切割结构 探针与靶核酸的结合并且还包含结合部分。 本发明还包括用核酸酶切割切割结构以释放核酸片段以产生信号的步骤，其中产生信号指示样品中靶核酸的存在，以及检测和/或测量 通过结合部分与固体支持物上的捕获元件结合捕获的片段的量。 本发明还涉及一种检测或测量样品中的靶核酸的方法，其中该方法包括通过将含有靶核酸的样品与具有在探针结合后变化的二级结构的探针形成切割结构 并且包含结合部分，并用核酸酶切割切割结构以产生切割的核酸片段，并通过结合部分与捕获元件的结合捕获的片段的量，并检测和/或测量 坚实的支持。