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公开(公告)号：US08852948B2
公开(公告)日：2014-10-07
申请号：US12185575
申请日：2008-08-04
CPC分类号： G01N33/6803 , G01N33/52
摘要： A protein analysis instrument and method are disclosed. The method includes mixing a binding dye composition and a protein sample using a homogenizer, measuring a parameter selected from the group consisting of the speed of the homogenizer and the resistance of the mixture to the homogenizer, adjusting the speed of the homogenizer based upon the measured parameter, pumping unreacted dye composition from the mixture and to a calorimeter, and measuring the absorbance of the dye composition in the calorimeter. Aspects of the invention also include inserting a spout into a sample cup at a position where the spout opening is positioned to avoid foam and precipitate generated by the mixing step and above the bottom of the sample cup and thereafter pumping the dye composition from the mixture in the sample cup through the spout and to a colorimeter.
摘要翻译： 公开了一种蛋白质分析仪器和方法。 该方法包括使用均化器混合结合染料组合物和蛋白质样品，测量选自均化器的速度和混合物对均化器的电阻的参数，基于测量的调节匀浆器的速度 参数，从混合物和比色计中抽出未反应的染料组合物，并测量色度计中染料组合物的吸光度。 本发明的方面还包括将喷口插入样品杯中，在出口开口的位置处，以避免由混合步骤产生的泡沫和沉淀，并且在样品杯的底部之上，然后从混合物中抽出染料组合物 样品杯通过喷口和色度计。
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公开(公告)号：US20100291686A1
公开(公告)日：2010-11-18
申请号：US12721688
申请日：2010-03-11
CPC分类号： G01N33/6839 , G01N1/286 , G01N21/17 , G01N21/251 , G01N33/12 , G01N33/6803 , G01N35/1095 , G01N2001/4088 , Y10T436/10 , Y10T436/105831 , Y10T436/25
摘要： A dye binding method for protein analysis is disclosed. The method includes the steps of preparing an initial reference dye solution of unknown concentration from an initial reference dye concentrate and creating an electronic signal based upon the absorbance of the initial reference dye solution. Thereafter, an electronic signal is created based upon the absorbance of a dye filtrate solution prepared from the initial reference dye solution and an initial protein sample. The absorbance signals from the reference dye solution and the dye filtrate solution are sent to a processor that compares the respective absorbances and calculates the protein content of the protein sample based upon the difference between the absorbances. An electronic signal is created based upon the absorbance of a successive dye filtrate solution prepared from the reference dye solution and a successive protein sample, and the absorbance signal from the successive sample dye filtrate solution is sent to the processor to calculate the protein content of the successive sample based upon the difference between the absorbance of the initial reference dye solution and the absorbance of the successive dye filtrate solution.
摘要翻译： 公开了用于蛋白质分析的染料结合方法。 该方法包括以下步骤：从初始参考染料浓缩物制备未知浓度的初始参考染料溶液，并基于初始参考染料溶液的吸光度产生电子信号。 此后，基于由初始参考染料溶液和初始蛋白质样品制备的染料滤液的吸光度，产生电子信号。 将来自参考染料溶液和染料滤液的吸光度信号送至处理器，该处理器根据吸光度之差来比较各吸光度，并计算蛋白质样品的蛋白质含量。 基于由参考染料溶液和连续蛋白质样品制备的连续染料滤液的吸光度产生电子信号，并将来自连续样品染料滤液的溶液的吸光度信号发送到处理器，以计算蛋白质含量 基于初始参考染料溶液的吸光度与连续的染料滤液的吸光度之间的差异的连续样品。
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公开(公告)号：US08147759B2
公开(公告)日：2012-04-03
申请号：US11846598
申请日：2007-08-29
IPC分类号： G01N21/78
CPC分类号： G01N33/6839 , G01N1/286 , G01N21/17 , G01N21/251 , G01N33/12 , G01N33/6803 , G01N35/1095 , G01N2001/4088 , Y10T436/10 , Y10T436/105831 , Y10T436/25
摘要： A direct rapid automated protein analyzer is disclosed. The protein analyzer includes means for reducing protein samples to small particles, a reaction vessel in material transfer communication with the homogenizer, a reservoir for binding dye composition in fluid communication with the reaction vessel, a metering pump in fluid communication with the reservoir and the reaction vessel for distributing discrete predetermined amounts of a binding dye composition to the reaction vessel, a filter in fluid communication with the reaction vessel for separating solids from filtrate after a dye binding reaction has taken place in the reaction vessel, and a calorimeter in fluid communication with the filter and the reaction vessel for measuring the absorbance of the filtrate from the reaction vessel and the filter. The rapid analyzer can be used in conjunction with a kit that includes a sample cup for mixing a protein sample with a dye-binding solution and a filter holder for being positioned in the sample cup. The filter holder includes a filter media and a depending spout below the filter media that reaches bottom portions of the cup when the filter media is positioned above the cup. The kit can also include dye concentrate solution.
摘要翻译： 公开了一种直接快速自动蛋白质分析仪。 蛋白质分析仪包括将蛋白质样品还原成小颗粒的装置，与均化器材料转移连通的反应容器，用于结合与反应容器流体连通的染料组合物的储存器，与储存器流体连通的计量泵和反应 用于将分散的预定量的结合染料组合物分配到反应容器中的过滤器，与反应容器流体连通的过滤器，用于在反应容器中发生染料结合反应后从滤液中分离固体;以及色度计，其与 过滤器和用于测量来自反应容器和过滤器的滤液的吸光度的反应容器。 快速分析仪可以与包括用于将蛋白质样品与染料结合溶液混合的样品杯和用于定位在样品杯中的过滤器保持器的试剂盒结合使用。 当过滤介质位于杯子上方时，过滤器保持器包括过滤介质和在过滤介质下方到达杯的底部的垂直喷口。 该试剂盒还可以包括染料浓缩液。
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公开(公告)号：US20090061522A1
公开(公告)日：2009-03-05
申请号：US11846598
申请日：2007-08-29
CPC分类号： G01N33/6839 , G01N1/286 , G01N21/17 , G01N21/251 , G01N33/12 , G01N33/6803 , G01N35/1095 , G01N2001/4088 , Y10T436/10 , Y10T436/105831 , Y10T436/25
摘要： A direct rapid automated protein analyzer is disclosed. The protein analyzer includes means for reducing protein samples to small particles, a reaction vessel in material transfer communication with the homogenizer, a reservoir for binding dye composition in fluid communication with the reaction vessel, a metering pump in fluid communication with the reservoir and the reaction vessel for distributing discrete predetermined amounts of a binding dye composition to the reaction vessel, a filter in fluid communication with the reaction vessel for separating solids from filtrate after a dye binding reaction has taken place in the reaction vessel, and a calorimeter in fluid communication with the filter and the reaction vessel for measuring the absorbance of the filtrate from the reaction vessel and the filter. The rapid analyzer can be used in conjunction with a kit that includes a sample cup for mixing a protein sample with a dye-binding solution and a filter holder for being positioned in the sample cup. The filter holder includes a filter media and a depending spout below the filter media that reaches bottom portions of the cup when the filter media is positioned above the cup. The kit can also include dye concentrate solution.
摘要翻译： 公开了一种直接快速自动蛋白质分析仪。 蛋白质分析仪包括将蛋白质样品还原成小颗粒的装置，与均化器材料转移连通的反应容器，用于结合与反应容器流体连通的染料组合物的储存器，与储存器流体连通的计量泵和反应 用于将分散的预定量的结合染料组合物分配到反应容器中的过滤器，与反应容器流体连通的过滤器，用于在反应容器中发生染料结合反应后从滤液中分离固体;以及色度计，其与 过滤器和用于测量来自反应容器和过滤器的滤液的吸光度的反应容器。 快速分析仪可以与包括用于将蛋白质样品与染料结合溶液混合的样品杯和用于定位在样品杯中的过滤器保持器的试剂盒结合使用。 当过滤介质位于杯子上方时，过滤器保持器包括过滤介质和在过滤介质下方到达杯的底部的垂直喷口。 该试剂盒还可以包括染料浓缩液。
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公开(公告)号：US20100290949A1
公开(公告)日：2010-11-18
申请号：US12721993
申请日：2010-03-11
IPC分类号： B01J19/00
CPC分类号： G01N33/6839 , G01N1/286 , G01N21/17 , G01N21/251 , G01N33/12 , G01N33/6803 , G01N35/1095 , G01N2001/4088 , Y10T436/10 , Y10T436/105831 , Y10T436/25
摘要： A protein analysis kit is disclosed. The kit includes a sample cup for mixing a protein sample with a dye-binding solution and a filter holder for being positioned in the sample cup. The filter holder includes a filter media and a depending spout below the filter media that reaches bottom portions of the cup when the filter media is positioned above the cup.
摘要翻译： 公开了蛋白质分析试剂盒。 该试剂盒包括用于将蛋白质样品与染料结合溶液混合的样品杯和用于定位在样品杯中的过滤器保持器。 当过滤介质位于杯子上方时，过滤器保持器包括过滤介质和在过滤介质下方到达杯的底部的垂直喷口。
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公开(公告)号：US20090087917A1
公开(公告)日：2009-04-02
申请号：US12185575
申请日：2008-08-04
IPC分类号： G01N21/78
CPC分类号： G01N33/6803 , G01N33/52
摘要： A protein analysis instrument and method are disclosed. The method includes mixing a binding dye composition and a protein sample using a homogenizer, measuring a parameter selected from the group consisting of the speed of the homogenizer and the resistance of the mixture to the homogenizer, adjusting the speed of the homogenizer based upon the measured parameter, pumping unreacted dye composition from the mixture and to a calorimeter, and measuring the absorbance of the dye composition in the calorimeter. Aspects of the invention also include inserting a spout into a sample cup at a position where the spout opening is positioned to avoid foam and precipitate generated by the mixing step and above the bottom of the sample cup and thereafter pumping the dye composition from the mixture in the sample cup through the spout and to a colorimeter.
摘要翻译： 公开了一种蛋白质分析仪器和方法。 该方法包括使用均化器混合结合染料组合物和蛋白质样品，测量选自均化器的速度和混合物对均化器的电阻的参数，基于测量的调节匀浆器的速度 参数，从混合物和比色计中抽出未反应的染料组合物，并测量色度计中染料组合物的吸光度。 本发明的方面还包括将喷口插入样品杯中，在出口开口的位置处，以避免由混合步骤产生的泡沫和沉淀，并且在样品杯的底部之上，然后从混合物中抽出染料组合物 样品杯通过喷口和色度计。
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公开(公告)号：US20110036705A1
公开(公告)日：2011-02-17
申请号：US12541262
申请日：2009-08-14
CPC分类号： G01N1/44 , B01J19/126 , B01J2219/0801 , B01J2219/0871 , B01J2219/0877 , B01J2219/089 , B01J2219/1215 , B01J2219/1233 , B01J2219/1236 , B01J2219/1242 , B01J2219/1275 , H05B6/6447 , H05B6/806
摘要： An instrument and method for high pressure microwave assisted chemistry are disclosed. The method includes the steps of applying microwave radiation to a sample in a sealed vessel while measuring the temperature of the sample and measuring the pressure generated inside the vessel and until the measured pressure reaches a designated set point, opening the vessel to release gases until the pressure inside the vessel reaches a lower designated set point, closing the vessel, and repeating the steps of opening the vessel at designated pressure set points and closing the vessel at designated pressure set points to the sample until the sample reaction reaches a designated high temperature. The designated set points can controllably differ from one another as the reaction proceeds. Microwave energy can be applied continuously or intermittently during the opening and closing steps. The apparatus includes a microwave cavity, a microwave transparent pressure resistant reaction vessel in the cavity, a cap on the reaction vessel, a pressure sensor for measuring pressure in the vessel, a temperature sensor, and means for opening and closing the cap at predetermined pressure set points measured by the pressure sensor to release pressure from the vessel.
摘要翻译： 公开了一种用于高压微波辅助化学的仪器和方法。 该方法包括以下步骤：在测量样品的温度并测量容器内部产生的压力并直到测量的压力达到指定的设定点，将微波辐射施加到密封容器中的样品中，打开容器释放气体直至 容器内的压力达到下指定设定点，关闭容器，并重复在指定压力设定点打开容器的步骤，并在指定的压力设定点关闭容器至样品，直到样品反应达到指定的高温。 当反应进行时，指定的设定点可以彼此可控地不同。 微波能量可以在打开和关闭步骤期间连续或间歇地施加。 该装置包括微波空腔，空腔中的微波透明耐压反应容器，反应容器上的盖，用于测量容器压力的压力传感器，温度传感器以及用于以预定压力打开和关闭盖的装置 由压力传感器测量的设定点以释放来自容器的压力。
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公开(公告)号：US20120074136A1
公开(公告)日：2012-03-29
申请号：US13239447
申请日：2011-09-22
IPC分类号： H05B6/64
CPC分类号： G01N1/44 , B01J19/126 , B01J2219/0801 , B01J2219/0871 , B01J2219/0877 , B01J2219/089 , B01J2219/1215 , B01J2219/1233 , B01J2219/1236 , B01J2219/1242 , B01J2219/1275 , H05B6/6447 , H05B6/806
摘要： An instrument and method for high pressure microwave assisted chemistry are disclosed. The method includes the steps of applying microwave radiation to a sample in a sealed vessel while measuring the temperature and pressure generated inside the vessel and until the measured pressure reaches a designated set point, opening the vessel to release gases until the pressure inside the vessel reaches a lower set point, closing the vessel, and repeating the steps of opening the vessel at designated pressure set points and closing the vessel at designated pressure set points to the sample until the sample reaction reaches a designated high temperature. Microwave energy can be applied continuously or intermittently during the opening and closing steps. Among other items, the instrument includes pressure and temperature sensors and means for opening and closing the vessel at the set points.
摘要翻译： 公开了一种用于高压微波辅助化学的仪器和方法。 该方法包括以下步骤：在测量容器内部产生的温度和压力的同时，将测量的压力达到指定的设定点，向容器中的样品施加微波辐射，打开容器释放气体，直到容器内的压力达到 下部设定点，关闭容器，并重复在指定的压力设定点打开容器的步骤，并在指定的压力设定点关闭容器至样品，直到样品反应达到指定的高温。 微波能量可以在打开和关闭步骤期间连续或间歇地施加。 在其他项目中，仪器包括压力和温度传感器以及用于在设定点打开和关闭容器的装置。
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公开(公告)号：US09161395B2
公开(公告)日：2015-10-13
申请号：US13173534
申请日：2011-06-30
申请人： Joseph J. Lambert
发明人： Joseph J. Lambert
CPC分类号： H05B6/806 , H05B6/6438 , H05B6/6441 , H05B6/6447 , H05B6/645 , H05B6/6464 , H05B6/68
摘要： An instrument for performing microwave-assisted reactions typically includes (i) a microwave-radiation source, (ii) a cavity, (iii) a waveguide in microwave communication with the microwave-radiation source and the cavity, (iv) at least one reaction-vessel sensor for determining the number and/or type of reaction vessels positioned within the cavity, (v) an interface, and (vi) a computer controller. The computer controller is typically in communication with the interface, the microwave-radiation source, and the reaction-vessel sensor. The computer controller is typically capable of determining the output of the microwave-radiation source in response to the number and/or type of reaction vessels positioned within the cavity.
摘要翻译： 用于进行微波辅助反应的仪器通常包括（i）微波辐射源，（ii）空腔，（iii）与微波辐射源和空腔微波通信的波导，（iv）至少一个反应 - vessel传感器，用于确定位于腔内的反应容器的数量和/或类型，（v）界面，和（vi）计算机控制器。 计算机控制器通常与接口，微波辐射源和反应容器传感器通信。 计算机控制器通常能够响应于位于空腔内的反应容器的数量和/或类型来确定微波辐射源的输出。
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公开(公告)号：US20130001221A1
公开(公告)日：2013-01-03
申请号：US13173534
申请日：2011-06-30
申请人： Joseph J. Lambert
发明人： Joseph J. Lambert
IPC分类号： H05B6/50
CPC分类号： H05B6/806 , H05B6/6438 , H05B6/6441 , H05B6/6447 , H05B6/645 , H05B6/6464 , H05B6/68
摘要： An instrument for performing microwave-assisted reactions and an associated method are disclosed. The instrument typically includes (i) a microwave-radiation source, (ii) a cavity, (iii) a waveguide in microwave communication with the microwave-radiation source and the cavity, (iv) at least one reaction-vessel sensor for determining the number and/or type of reaction vessels positioned within the cavity, (v) an interface, and (vi) a computer controller. The computer controller is typically in communication with the interface, the microwave-radiation source, and the reaction-vessel sensor. The computer controller is typically capable of determining the output of the microwave-radiation source in response to the number and/or type of reaction vessels positioned within the cavity.
摘要翻译： 公开了用于进行微波辅助反应的仪器和相关方法。 仪器通常包括（i）微波辐射源，（ii）空腔，（iii）与微波辐射源和空腔微波通信的波导，（iv）至少一个反应容器传感器，用于确定 位于腔内的反应容器的数量和/或类型，（v）界面，和（vi）计算机控制器。 计算机控制器通常与接口，微波辐射源和反应容器传感器通信。 计算机控制器通常能够响应于位于空腔内的反应容器的数量和/或类型来确定微波辐射源的输出。
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