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    Enzyme feed additive and animal feed including it
    2.
    发明授权
    Enzyme feed additive and animal feed including it 有权
    酶饲料添加剂和动物饲料包括它

    公开(公告)号：US08715647B2

    公开(公告)日：2014-05-06

    申请号：US11198426

    申请日：2005-08-05

    申请人： Michael R. Bedford ,  Andrew J. Morgan ,  Timothy Fowler ,  Kathleen A. Clarkson ,  Michael Ward ,  Katherine D. Collier ,  Edmund A. Larenas

    发明人： Michael R. Bedford ,  Andrew J. Morgan ,  Timothy Fowler ,  Kathleen A. Clarkson ,  Michael Ward ,  Katherine D. Collier ,  Edmund A. Larenas

    IPC分类号： A23K1/165 ,  A61K38/54 ,  A61K38/47 ,  A61K38/46 ,  A61K38/00

    CPC分类号： C12Y302/01004 ,  A23K20/189 ,  A23K50/75 ,  C12N9/2437 ,  C12Y302/01091

    摘要： The use is provided composition as a feed additive which comprises one or more endoglucanases, and 0-20% by weight, based upon the content of cellulase proteins in the composition, of a cellobiohydrolase. The endoglycanases may be one or more of EGI, EGII, EGIII and any functionally active derivative of any thereof. Such endoglucanases may be obtained from a genetically modified strain of the fungus Trichoderma. Also provided is an enzyme-based feed additive which comprises EGI and/or EGII which lack the cellulose binding domain, and 0-20% by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase. A further enzyme-based feed additive is provided which comprises a cereal-based carrier, one or more endoglucanases, and 0-20% by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase.

    摘要翻译： 提供的组合物作为饲料添加剂，其包含一种或多种内切葡聚糖酶，并且基于组合物中纤维素酶蛋白质的含量为0-20重量％的纤维二糖水解酶。 所述凝集素可以是EGI，EGII，EGIII及其任何的任何功能活性衍生物中的一种或多种。 这样的内切葡聚糖酶可以从真菌木霉的遗传修饰菌株获得。 还提供了一种基于酶的饲料添加剂，其包含缺乏纤维素结合结构域的EGI和/或EGII，并且基于添加剂中纤维素酶蛋白质的含量为0-20重量％的纤维二糖水解酶。 提供了另外一种基于酶的饲料添加剂，其包含基于谷物的载体，一种或多种内切葡聚糖酶，和基于添加剂中的纤维素酶蛋白质含量为0-20重量％的纤维二糖水解酶。

    Enzyme feed additive and animal feed including it
    4.
    发明授权
    Enzyme feed additive and animal feed including it 失效
    酶饲料添加剂和动物饲料包括它

    公开(公告)号：US07005128B1

    公开(公告)日：2006-02-28

    申请号：US10379978

    申请日：2003-03-04

    申请人： Michael R. Bedford ,  Andrew J. Morgan ,  Timothy Fowler ,  Kathleen A. Clarkson ,  Michael Ward ,  Katherine D. Collier ,  Edmund A Larenas

    发明人： Michael R. Bedford ,  Andrew J. Morgan ,  Timothy Fowler ,  Kathleen A. Clarkson ,  Michael Ward ,  Katherine D. Collier ,  Edmund A Larenas

    IPC分类号： A61K38/47 ,  A61K38/43 ,  A61K38/54 ,  A23K1/00

    CPC分类号： C12Y302/01004 ,  A23K20/189 ,  A23K50/75 ,  C12N9/2437 ,  C12Y302/01091

    摘要： The use is provided of a composition as a feed additive which comprises one or more endoglucanases, and 0–20% by weight, based upon the content of cellulase proteins in the composition, of a cellobiohydrolase. The endoglycanases may be one or more of EGI, EGII, EGIII and any functionally active derivative of any thereof. Such endoglucanases may be obtained from a genetically modified strain of the fungus Trichoderma. Also provided is an enzyme-based feed additive which comprises EGI and/or EGII which lack the cellulose binding domain, and 0–20% by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase. A further enzyme-based feed additive is provided which comprises a cereal-based carrier, one or more endoglucanases, and 0–20% by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase. Such enzyme-based feed additives can be incorporated into a cereal-based feed which includes one or more of barley, wheat, triticale, rye and maize. The feed additive has the advantage of improving the feed conversion ratio and/or increasing the digestibility of a cereal-based feed in which it is included.

    摘要翻译： 提供的组合物作为饲料添加剂，其包含一种或多种内切葡聚糖酶，并且基于组合物中纤维素酶蛋白质的含量为0-20重量％的纤维二糖水解酶。 所述凝集素可以是EGI，EGII，EGIII及其任何的任何功能活性衍生物中的一种或多种。 这样的内切葡聚糖酶可以从真菌木霉的遗传修饰菌株获得。 还提供了一种基于酶的饲料添加剂，其包含缺乏纤维素结合结构域的EGI和/或EGII，并且基于添加剂中纤维素酶蛋白质的含量为0-20重量％的纤维二糖水解酶。 提供了另外一种基于酶的饲料添加剂，其包含基于谷物的载体，一种或多种内切葡聚糖酶，和基于添加剂中的纤维素酶蛋白质含量为0-20重量％的纤维二糖水解酶。 这种基于酶的饲料添加剂可以掺入包括大麦，小麦，黑小麦，黑麦和玉米中的一种或多种的谷类饲料中。 饲料添加剂具有提高饲料转化率和/或增加其中包含谷物饲料的消化率的优点。

    Enzyme feed additive and animal feed including it
    5.
    发明授权
    Enzyme feed additive and animal feed including it 失效
    酶饲料添加剂和动物饲料包括它

    公开(公告)号：US06562340B1

    公开(公告)日：2003-05-13

    申请号：US08507362

    申请日：1995-08-16

    申请人： Michael R. Bedford ,  Andrew J. Morgan ,  Timothy Fowler ,  Kathleen A. Clarkson ,  Michael Ward ,  Katherine D. Collier ,  Edmund A Larenas

    发明人： Michael R. Bedford ,  Andrew J. Morgan ,  Timothy Fowler ,  Kathleen A. Clarkson ,  Michael Ward ,  Katherine D. Collier ,  Edmund A Larenas

    IPC分类号： A61K3847

    CPC分类号： C12Y302/01004 ,  A23K20/189 ,  C12N9/2437 ,  C12N15/80 ,  C12Y302/01021 ,  C12Y302/01091

    摘要： The use is provided of a composition as a feed additive which comprises one or more endoglucanases, and 0-20% by weight, based upon the content of cellulase proteins in the composition, of a cellobiohydrolase. The endoglycanases may be one or more of EGI, EGII EGIII and any functionally active derivative of any thereof. Such endoglucanases may be obtained from a genetically modified strain of the fungus Trichoderma. Also provided is an enzyme-based feed additive which comprises EGI and/or EGII which lack the cellulose binding domain, and 0-20% by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase. A further enzyme-based feed additive is provided which comprises a cereal-based carrier, one or more endoglucanases, and 0-20% by weight, based upon the content of cellulase proteins in the additive, of a cellobiohydrolase. Such enzyme-based feed additives can be incorporated into a cereal-based feed which includes one or more of barley, wheat, tricale, rye and maize. The feed additive has the advantage of improving the feed conversion ratio and/or increasing the digestibility of a cereal-based feed in which it is included.

    摘要翻译： 提供的组合物作为饲料添加剂，其包含一种或多种内切葡聚糖酶，并且基于组合物中纤维素酶蛋白质的含量为0-20重量％的纤维二糖水解酶。 所述凝集素可以是一种或多种EGI，EGII EGIII及其任何功能活性的衍生物。 这样的内切葡聚糖酶可以从真菌木霉的遗传修饰菌株获得。 还提供了一种基于酶的饲料添加剂，其包含缺乏纤维素结合结构域的EGI和/或EGII，并且基于添加剂中纤维素酶蛋白质的含量为0-20重量％的纤维二糖水解酶。 提供了另外一种基于酶的饲料添加剂，其包含基于谷物的载体，一种或多种内切葡聚糖酶，和基于添加剂中的纤维素酶蛋白质含量为0-20重量％的纤维二糖水解酶。 这种基于酶的饲料添加剂可以掺入包括一种或多种大麦，小麦，三倍体，黑麦和玉米的谷类饲料中。 饲料添加剂具有提高饲料转化率和/或增加其中包含谷物饲料的消化率的优点。

    Thermostable xylanases from Microtetraspora flexuosa as a feed additive
    7.
    发明授权
    Thermostable xylanases from Microtetraspora flexuosa as a feed additive 失效
    作为饲料添加剂来自Microtetraspora flexuosa的热稳定性木聚糖酶

    公开(公告)号：US6132716A

    公开(公告)日：2000-10-17

    申请号：US732242

    申请日：1997-04-07

    申请人： Andrew J. Morgan ,  Kathleen A. Clarkson ,  Elizabeth A. Bodie ,  William A. Cuevas

    发明人： Andrew J. Morgan ,  Kathleen A. Clarkson ,  Elizabeth A. Bodie ,  William A. Cuevas

    IPC分类号： A23K1/165 ,  C12N9/24 ,  C12P21/00 ,  C12R1/00 ,  C12R1/645 ,  C12S3/08 ,  D21C5/00 ,  D21C9/10 ,  A61K38/47 ,  A23L1/28

    CPC分类号： C12Y302/01032 ,  C12N9/248 ,  C12Y302/01008 ,  D21C5/005 ,  Y10S426/807 ,  Y10S435/822

    摘要： A feed additive that provides increased feed conversion ratio is prepared containing a physiologically acceptable carrier and one or more of five thermostable xylanases from Microtetraspora flexuosa, preferably strain ATCC 35864. The xylanases can tolerate heating for one minute in a water bath at 95.degree. C. without substantial loss of activity, and tolerate alkaline conditions in a pH range of 7.0-10.0. A feed for poultry or pigs contains the xylanases in a feed containing at least 20% by weight cereal. The five xylanases have the following characteristics: (i) a molecular weight of about 33,100 daltons, a pI of about 8.5, and a maximum activity at pH 7.0-7.5 at a temperature of about 70.degree. C.; (ii) a molecular weight of about 33,300 daltons, a pI of about 7.5, and a maximum activity at pH 7.0-7.5 at a temperature of about 65.degree. C.; (iii) a molecular weight of about 31,000 daltons, a pI of about 6.2, and a maximum activity at pH 7.5 at a temperature of about 65.degree. C.; (iv) a molecular weight of about 50,000 daltons, a pI of about 5.8, and a maximum activity at pH 7.5 at a temperature of about 65.degree. C.; and (v) a molecular weight of about 35,000 daltons, a pI of about 5.3, and a maximum activity at pH 7.5 at a temperature of about 70.degree. C.

    摘要翻译： PCT No.PCT / EP95 / 01628 Sec。 371日期1997年4月7日 102（e）日期1997年4月7日PCT 1995年4月28日PCT PCT。 WO95 / 29997 PCT出版物 日期1995年11月9日制备提供增加的饲料转化率的饲料添加剂，其含有生理学上可接受的载体和来自Microtetraspora flexuosa的五种热稳定性木聚糖酶中的一种或多种，​​优选菌株ATCC 35864.木聚糖酶可以耐受在水浴中加热1分钟 在95℃下没有显着的活性损失，并且在7.0-10.0的pH范围内耐受碱性条件。 家禽或猪的饲料在含有至少20重量％谷物的饲料中含有木聚糖酶。 五种木聚糖酶具有以下特征：（i）约33,100道尔顿的分子量，约8.5的pI值，在约70℃的温度下在pH 7.0-7.5的最大活性。 （ii）约33,300道尔顿的分子量，约7.5的pI，在约65℃的温度下在pH 7.0-7.5的最大活性。 （iii）约31,000道尔顿的分子量，约6.2的pI，在约65℃的温度下在7.5的最大活性。 （iv）约50,000道尔顿的分子量，约5.8的pI，在约65℃的温度下在pH7.5的最大活性。 和（v）约35,000道尔顿的分子量，约5.3的pI，在约70℃的温度下在pH7.5的最大活性。