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    DNA encoding enzyme recombinant DNA and enzyme transformant, and their preparation and uses
    4.
    发明授权
    DNA encoding enzyme recombinant DNA and enzyme transformant, and their preparation and uses 失效
    DNA编码酶重组DNA和酶转化体，及其制备及应用

    公开(公告)号：US5871977A

    公开(公告)日：1999-02-16

    申请号：US714677

    申请日：1996-09-16

    申请人： Michio Kubota ,  Keiji Tsusaki ,  Kazuhiko Maruta ,  Toshiyuki Sugimoto

    发明人： Michio Kubota ,  Keiji Tsusaki ,  Kazuhiko Maruta ,  Toshiyuki Sugimoto

    IPC分类号： C12N1/21 ,  C12N9/90 ,  C12N15/56 ,  C12P19/12 ,  C07H21/04 ,  C12N9/24

    CPC分类号： C12P19/12 ,  C12N9/90

    摘要： A DNA encoding an enzyme, which forms non-reducing saccharides having trehalose structure as an end unit from amylaceous saccharides having a degree of glucose polymerization of 3 or higher, enables an industrial-scale production of a recombinant enzyme with such enzyme activity. Non-reducing saccharides obtainable by the recombinant enzyme can be used in a variety of food products, cosmetics, pharmaceuticals and feeds because of their substantial non-reducibility, mild and high-quality sweetness, adequate viscosity, and moisture-retaining ability.

    摘要翻译： 从葡萄糖聚合度为3以上的淀粉糖形成具有海藻糖结构的非还原性糖类作为末端单元的酶的DNA能够实现具有这种酶活性的重组酶的工业规模生产。 可以通过重组酶获得的非还原性糖可以用于各种食品，化妆品，药​​物和饲料中，因为它们具有显着的不可还原性，温和和高品质的甜度，足够的粘度和保湿能力。

    DNA encoding enzyme, recombinant DNA and enzyme, transformant, and their preparation and uses
    6.
    发明授权
    DNA encoding enzyme, recombinant DNA and enzyme, transformant, and their preparation and uses 失效
    DNA编码酶，重组DNA和酶，转化体及其制备和用途

    公开(公告)号：US5871994A

    公开(公告)日：1999-02-16

    申请号：US714537

    申请日：1996-09-16

    申请人： Michio Kubota ,  Keiji Tsusaki ,  Kazuhiko Maruta ,  Toshiyuki Sugimoto

    发明人： Michio Kubota ,  Keiji Tsusaki ,  Kazuhiko Maruta ,  Toshiyuki Sugimoto

    IPC分类号： C12N1/21 ,  C12N9/90 ,  C12N15/56 ,  C12P19/12 ,  C12N9/24 ,  C12N9/26

    CPC分类号： C12P19/12 ,  C12N9/90

    摘要： A DNA encoding an enzyme, which forms non-reducing saccharides having trehalose structure as an end unit from amylaceous saccharides having a degree of glucose polymerization of 3 or higher, enables an industrial-scale production of a recombinant enzyme with such enzyme activity. Non-reducing saccharides obtainable by the recombinant enzyme can be used in a variety of food products, cosmetics, pharmaceuticals and feeds because of their substantial non-reducibility, mild and high-quality sweetness, adequate viscosity, and moisture-retaining ability.

    摘要翻译： 从葡萄糖聚合度为3以上的淀粉糖形成具有海藻糖结构的非还原性糖类作为末端单元的酶的DNA能够实现具有这种酶活性的重组酶的工业规模生产。 可以通过重组酶获得的非还原性糖可以用于各种食品，化妆品，药​​物和饲料中，因为它们具有显着的不可还原性，温和和高品质的甜度，足够的粘度和保湿能力。

    Recombinant enzyme for converting maltose into trehalose from pimelobacter sp.
    7.
    发明授权
    Recombinant enzyme for converting maltose into trehalose from pimelobacter sp. 失效
    用于从麦芽糖转化成麦芽糖至海藻糖的重组酶。

    公开(公告)号：US5763228A

    公开(公告)日：1998-06-09

    申请号：US528199

    申请日：1995-09-14

    申请人： Michio Kubota ,  Keiji Tsusaki ,  Toshiyuki Sugimoto

    发明人： Michio Kubota ,  Keiji Tsusaki ,  Toshiyuki Sugimoto

    IPC分类号： C12N9/90 ,  C12P19/24 ,  C12P19/12 ,  C12N9/24 ,  C12N15/56

    CPC分类号： C12N9/90 ,  C12P19/24

    摘要： A recombinant enzyme, having a molecular weight of about 57,000-67,000 daltons on SDS-PAGE and a pI of about 4.1-5.1 on isoelectrophoresis, which converts maltose into trehalose and vice versa. Depending on the enzymatic conditions, the enzyme forms about 70 w/w % of trehalose when acts on maltose, while about 20 w/w % of maltose when acts on trehalose. The culture of a transformant, prepared by introducing into a host a recombinant DNA containing a DNA coding for the enzyme and a self-replicable vector, facilitates the industrial-scale production of trehalose.

    摘要翻译： 重组酶，SDS-PAGE上分子量约57,000-67,000道尔顿，在电泳上的pI约为4.1-5.1，将麦芽糖转化为海藻糖，反之亦然。 根据酶的条件，当作用于麦芽糖时，该酶形成约70w / w％的海藻糖，而当作用于海藻糖时约为20w / w％的麦芽糖。 通过向宿主中引入含有编码酶的DNA和自我复制载体的重组DNA制备的转化体的培养有利于海藻糖的工业规模生产。