公开(公告)号：US20070238141A1

公开(公告)日：2007-10-11

申请号：US11729901

申请日：2007-03-30

申请人： Michitaka Notoya ,  Tomoko Ohyama ,  Shigeyuki Tamura ,  Tomokazu Yoshida ,  Hideki Ishihara

发明人： Michitaka Notoya ,  Tomoko Ohyama ,  Shigeyuki Tamura ,  Tomokazu Yoshida ,  Hideki Ishihara

IPC分类号： C12Q1/48 ,  G01N33/53

CPC分类号： C12Q1/485 ,  G01N33/573

摘要： A method for measuring the activity of a receptor kinase present in a cell membrane of a cell is described. The method comprises steps of preparing a sample containing the receptor kinase by separating a cytoplasm from the cell, contacting the receptor kinase in the sample with a substrate to phosphorylate the substrate with the activity of the receptor kinase, binding a labeling substance to the phosphorylated substrate, and measuring the activity of the receptor kinase based on a result of detection of the labeling substance bound to the phosphorylated substrate.

摘要翻译： 描述了测定存在于细胞的细胞膜中的受体激酶的活性的方法。 该方法包括以下步骤：通过从细胞分离细胞质来制备含有受体激酶的样品，使样品中的受体激酶与底物接触，以使受体激酶活性磷酸化底物，将标记物质结合到磷酸化底物 ，并且基于与磷酸化底物结合的标记物质的检测结果测量受体激酶的活性。

公开(公告)号：US08309298B2

公开(公告)日：2012-11-13

申请号：US11527584

申请日：2006-09-27

申请人： Shigeyuki Tamura ,  Tomokazu Yoshida

发明人： Shigeyuki Tamura ,  Tomokazu Yoshida

IPC分类号： C12Q1/00

CPC分类号： G01N33/573 ,  G01N33/54393 ,  G01N33/582 ,  G01N2333/912

摘要： A method for detecting an analyte in a sample is described herein. The method comprises: preparing a complex immobilized solid phase comprising a solid phase and a complex on the solid phase; the complex comprising an analyte and a signal emitting substance; treating the complex immobilized solid phase with a blocking agent; and detecting the analyte based on a signal emitted from the signal emitting substance of the complex immobilized solid phase.

摘要翻译： 本文描述了用于检测样品中分析物的方法。 该方法包括：在固相上制备包含固相和络合物的复合固定固相; 所述复合物包含分析物和信号发射物质; 用封闭剂处理复合物固定化固相; 以及基于从复合物固相固相的信号发射物质发射的信号检测分析物。

公开(公告)号：US20070105177A1

公开(公告)日：2007-05-10

申请号：US11527584

申请日：2006-09-27

申请人： Shigeyuki Tamura ,  Tomokazu Yoshida

发明人： Shigeyuki Tamura ,  Tomokazu Yoshida

IPC分类号： C12Q1/48

CPC分类号： G01N33/573 ,  G01N33/54393 ,  G01N33/582 ,  G01N2333/912

摘要： A method for detecting an analyte in a sample is described herein. The method comprises: preparing a complex immobilized solid phase comprising a solid phase and a complex on the solid phase; the complex comprising an analyte and a signal emitting substance; treating the complex immobilized solid phase with a blocking agent; and detecting the analyte based on a signal emitted from the signal emitting substance of the complex immobilized solid phase.

摘要翻译： 本文描述了用于检测样品中分析物的方法。 该方法包括：在固相上制备包含固相和络合物的复合物固定固相; 所述复合物包含分析物和信号发射物质; 用封闭剂处理复合物固定化固相; 以及基于从复合物固相固相的信号发射物质发射的信号检测分析物。

公开(公告)号：US06900671B2

公开(公告)日：2005-05-31

申请号：US10325902

申请日：2002-12-23

申请人： Hiroyuki Yamada ,  Shigeyuki Tamura ,  Kuniichi Ikemura

发明人： Hiroyuki Yamada ,  Shigeyuki Tamura ,  Kuniichi Ikemura

IPC分类号： H03F1/34 ,  H03F3/08 ,  H03G3/30 ,  H02M11/00

CPC分类号： H03G3/3084

摘要： It is an object of the present invention to provide a current-voltage conversion circuit in which the sensitivity varies in accordance with the amplitude of the input signal. In the current-voltage conversion circuit of the present invention, n+1 (n is an even number) amplifying inverters are connected in series between an input terminal and an output terminal; furthermore, the input of a negative feedback circuit constructed from an integrating circuit and a negative feedback inverter is connected to the output side of the nth-stage amplifying inverter, and the output of this negative feedback circuit is connected to the input side of the first-stage amplifying inverter. The integrating circuit outputs the mean value of the output potential of the nth-stage amplifying inverter into the negative feedback inverter, and the negative feedback inverter controls the current that flows the ground line from the input terminal in accordance with the output voltage of the integrating circuit. As a result, the sensitivity of the current-voltage conversion circuit varies accordingly.

摘要翻译： 本发明的目的是提供一种电流 - 电压转换电路，其中灵敏度根据输入信号的幅度而变化。 在本发明的电流 - 电压转换电路中，n + 1（n为偶数）放大逆变器串联连接在输入端子和输出端子之间; 此外，由积分电路和负反馈反相器构成的负反馈电路的输入连接到第n阶段放大反相器的输出侧，该负反馈电路的输出为 连接到第一级放大逆变器的输入侧。 积分电路将第n级阶段放大逆变器的输出电位的平均值输出到负反馈反相器，负反馈反相器控制从输入端子流过地线的电流 根据积分电路的输出电压。 结果，电流 - 电压转换电路的灵敏度相应地变化。