公开(公告)号：US07888635B2

公开(公告)日：2011-02-15

申请号：US12156360

申请日：2008-05-30

申请人： Mikhail E. Belov ,  Yehia M. Ibrahim ,  Biran H. Clowers ,  David C. Prior ,  Richard D. Smith

发明人： Mikhail E. Belov ,  Yehia M. Ibrahim ,  Biran H. Clowers ,  David C. Prior ,  Richard D. Smith

IPC分类号： H01J49/26

CPC分类号： H01J49/4235 ,  H01J49/066

摘要： An ion funnel trap is described that includes a inlet portion, a trapping portion, and a outlet portion that couples, in normal operation, with an ion funnel. The ion trap operates efficiently at a pressure of ˜1 Torr and provides for: 1) removal of low mass-to-charge (m/z) ion species, 2) ion accumulation efficiency of up to 80%, 3) charge capacity of ˜10,000,000 elementary charges, 4) ion ejection time of 40 to 200 μs, and 5) optimized variable ion accumulation times. Ion accumulation with low concentration peptide mixtures has shown an increase in analyte signal-to-noise ratios (SNR) of a factor of 30, and a greater than 10-fold improvement in SNR for multiply charged analytes.

摘要翻译： 描述了一种离子漏斗阱，其包括入口部分，捕获部分和出口部分，其在正常操作中与离子漏斗相连。 离子阱在〜1乇的压力下有效地工作，并提供：1）去除低质量 - 电荷（m / z）离子物质，2）离子累积效率高达80％，3）充电容量 〜10万次基本电荷，4）离子喷射时间为40〜200μs，5）优化的可变离子积聚时间。 具有低浓度肽混合物的离子累积显示出30倍的分析物信噪比（SNR）的增加，以及多次充电分析物的SNR的提高大于10倍。

公开(公告)号：US20090294644A1

公开(公告)日：2009-12-03

申请号：US12132303

申请日：2008-06-03

申请人： Mikhail E. Belov

发明人： Mikhail E. Belov

IPC分类号： B01D59/44

CPC分类号： H01J49/40 ,  G01N27/622 ,  H01J49/0031 ,  H01J49/066 ,  H01J49/4265

摘要： A method for multiplexed analysis using ion mobility spectrometer in which the effectiveness and efficiency of the multiplexed method is optimized by automatically adjusting rates of passage of analyte materials through an IMS drift tube during operation of the system. This automatic adjustment is performed by the IMS instrument itself after determining the appropriate levels of adjustment according to the method of the present invention. In one example, the adjustment of the rates of passage for these materials is determined by quantifying the total number of analyte molecules delivered to the ion trap in a preselected period of time, comparing this number to the charge capacity of the ion trap, selecting a gate opening sequence; and implementing the selected gate opening sequence to obtain a preselected rate of analytes within said IMS drift tube.

摘要翻译： 一种使用离子迁移谱仪进行复用分析的方法，其中通过在系统操作期间自动调节分析物质通过IMS漂移管的通过速率来优化复用方法的有效性和效率。 在根据本发明的方法确定适当的调整水平之后，由IMS仪器本身进行该自动调整。 在一个实例中，对这些材料的通过速率的调整通过在预选的时间段内量化输送到离子阱的分析物分子的总数来确定，将该数目与离子阱的电荷容量进行比较，选择 门打开序列; 并实施所选择的开门序列以获得所述IMS漂移管内的分析物的预选速率。

公开(公告)号：US08698075B2

公开(公告)日：2014-04-15

申请号：US13114869

申请日：2011-05-24

申请人： Ruwan T. Kurulugama ,  Mikhail E. Belov

发明人： Ruwan T. Kurulugama ,  Mikhail E. Belov

IPC分类号： H01J49/06

CPC分类号： H01J49/065 ,  H01J49/0404 ,  H01J49/044

摘要： An orthogonal ion injection apparatus and process are described in which ions are directly injected into an ion guide orthogonal to the ion guide axis through an inlet opening located on a side of the ion guide. The end of the heated capillary is placed inside the ion guide such that the ions are directly injected into DC and RF fields inside the ion guide, which efficiently confines ions inside the ion guide. Liquid droplets created by the ionization source that are carried through the capillary into the ion guide are removed from the ion guide by a strong directional gas flow through an inlet opening on the opposite side of the ion guide. Strong DC and RF fields divert ions into the ion guide. In-guide orthogonal injection yields a noise level that is a factor of 1.5 to 2 lower than conventional inline injection known in the art. Signal intensities for low m/z ions are greater compared to convention inline injection under the same processing conditions.

摘要翻译： 描述了一种正交离子注入装置和方法，其中离子通过位于离子导向器一侧的入口直接注入垂直于离子引导轴的离子导向器中。 加热的毛细管的末端放置在离子导向器内，使得离子直接注入到离子导向器内的DC和RF场中，这有效地将离子限制在离子导向器内。 由电离源产生的通过毛细管进入离子导向器的液滴通过强力的定向气流从离子导向器的相反侧的入口开口从离子导向器移除。 强直流和射频场将离子转移到离子导向器中。 导向正交注入产生比现有技术中已知的常规在线注入低1.5至2倍的噪声电平。 在相同的处理条件下，与常规的直线注入相比，低m / z离子的信号强度更大。

公开(公告)号：US08541737B2

公开(公告)日：2013-09-24

申请号：US12769268

申请日：2010-04-28

申请人： Yehia M. Ibrahim ,  Mikhail E. Belov ,  David C. Prior

发明人： Yehia M. Ibrahim ,  Mikhail E. Belov ,  David C. Prior

IPC分类号： H01J49/00

CPC分类号： H01J49/004

摘要： A collision cell is disclosed that provides ion activation in various selective modes. Ion activation is performed inside selected segments of a segmented quadrupole that provides maximum optimum capture and collection of fragmentation products. The invention provides collisional cooling of precursor ions as well as product fragments and further allows effective transmission of ions through a high pressure interface into a coupled mass analysis instrument.

摘要翻译： 公开了一种以各种选择模式提供离子激活的碰撞池。 离子激活在分段四极杆的选定区段内进行，提供最大限度的捕获和收集碎裂产物。 本发明提供前体离子以及产物碎片的碰撞冷却，并进一步允许离子通过高压界面有效传输到耦合的质量分析仪器中。

公开(公告)号：US20110127417A1

公开(公告)日：2011-06-02

申请号：US12769268

申请日：2010-04-28

申请人： Yehia M. Ibrahim ,  Mikhail E. Belov ,  David C. Prior

发明人： Yehia M. Ibrahim ,  Mikhail E. Belov ,  David C. Prior

IPC分类号： H01J49/40 ,  H01J49/02

CPC分类号： H01J49/004

摘要： A collision cell is disclosed that provides ion activation in various selective modes. Ion activation is performed inside selected segments of a segmented quadrupole that provides maximum optimum capture and collection of fragmentation products. The invention provides collisional cooling of precursor ions as well as product fragments and further allows effective transmission of ions through a high pressure interface into a coupled mass analysis instrument.

摘要翻译： 公开了一种以各种选择模式提供离子激活的碰撞池。 离子激活在分段四极杆的选定区段内进行，提供最大限度的捕获和收集碎裂产物。 本发明提供前体离子以及产物碎片的碰撞冷却，并进一步允许离子通过高压界面有效传输到耦合的质量分析仪器中。

公开(公告)号：US07718957B2

公开(公告)日：2010-05-18

申请号：US12132303

申请日：2008-06-03

申请人： Mikhail E. Belov

发明人： Mikhail E. Belov

IPC分类号： H01J49/40 ,  H01J49/26 ,  B01D59/44

CPC分类号： H01J49/40 ,  G01N27/622 ,  H01J49/0031 ,  H01J49/066 ,  H01J49/4265

摘要： A method for multiplexed analysis using ion mobility spectrometer in which the effectiveness and efficiency of the multiplexed method is optimized by automatically adjusting rates of passage of analyte materials through an IMS drift tube during operation of the system. This automatic adjustment is performed by the IMS instrument itself after determining the appropriate levels of adjustment according to the method of the present invention. In one example, the adjustment of the rates of passage for these materials is determined by quantifying the total number of analyte molecules delivered to the ion trap in a preselected period of time, comparing this number to the charge capacity of the ion trap, selecting a gate opening sequence; and implementing the selected gate opening sequence to obtain a preselected rate of analytes within said IMS drift tube.

摘要翻译： 一种使用离子迁移谱仪进行复用分析的方法，其中通过在系统操作期间自动调节分析物质通过IMS漂移管的通过速率来优化复用方法的有效性和效率。 在根据本发明的方法确定适当的调整水平之后，由IMS仪器本身进行该自动调整。 在一个实例中，对这些材料的通过速率的调整通过在预选的时间段内量化输送到离子阱的分析物分子的总数来确定，将该数目与离子阱的电荷容量进行比较，选择 门打开序列; 并实施所选择的开门序列以获得所述IMS漂移管内的分析物的预选速率。

公开(公告)号：US20120298853A1

公开(公告)日：2012-11-29

申请号：US13114869

申请日：2011-05-24

申请人： Ruwan T. Kurulugama ,  Mikhail E. Belov

发明人： Ruwan T. Kurulugama ,  Mikhail E. Belov

IPC分类号： H01J49/22 ,  H01J49/00

CPC分类号： H01J49/065 ,  H01J49/0404 ,  H01J49/044

摘要： An orthogonal ion injection apparatus and process are described in which ions are directly injected into an ion guide orthogonal to the ion guide axis through an inlet opening located on a side of the ion guide. The end of the heated capillary is placed inside the ion guide such that the ions are directly injected into DC and RF fields inside the ion guide, which efficiently confines ions inside the ion guide. Liquid droplets created by the ionization source that are carried through the capillary into the ion guide are removed from the ion guide by a strong directional gas flow through an inlet opening on the opposite side of the ion guide. Strong DC and RF fields divert ions into the ion guide. In-guide orthogonal injection yields a noise level that is a factor of 1.5 to 2 lower than conventional inline injection known in the art. Signal intensities for low m/z ions are greater compared to convention inline injection under the same processing conditions.

摘要翻译： 描述了一种正交离子注入装置和方法，其中离子通过位于离子导向器一侧的入口直接注入垂直于离子引导轴的离子导向器中。 加热的毛细管的末端放置在离子导向器内，使得离子直接注入到离子导向器内的DC和RF场中，这有效地将离子限制在离子导向器内。 由电离源产生的通过毛细管进入离子导向器的液滴通过强力的定向气流从离子导向器的相反侧的入口开口从离子导向器移除。 强直流和射频场将离子转移到离子导向器中。 导向正交注入产生比现有技术中已知的常规在线注入低1.5至2倍的噪声电平。 在相同的处理条件下，与常规的直线注入相比，低m / z离子的信号强度更大。

公开(公告)号：US20120261564A1

公开(公告)日：2012-10-18

申请号：US13086244

申请日：2011-04-13

申请人： Mikhail E. Belov

发明人： Mikhail E. Belov

IPC分类号： H01J49/26 ,  H01J49/40 ,  H01J49/00

CPC分类号： H01J49/0031 ,  G01N27/622 ,  H01J49/061

摘要： An apparatus and method for analyzing a sample containing multiple analytes that combines a separation device that separates the individual analytes by virtue of some physical and/or chemical characteristic other than the mass to charge ratio (m/z) interfaced with a mass spectrometer that detects the m/z of individual analytes. Separation is performed on the shorter timescale than signal detection with the mass spectrometer. A preferred embodiment utilizes an ion mobility spectrometer interfaced with an Orbitrap mass spectrometer.

摘要翻译： 一种用于分析含有多个分析物的样品的装置和方法，所述样品组合了分离装置，所述分离装置通过除与检测到的质谱仪（m / z）接口的质荷比（m / z）之外的某些物理和/或化学特性而分离各个分析物 单个分析物的m / z。 在比质谱仪的信号检测更短的时间刻度上进行分离。 优选的实施方案利用与Orbitrap质谱仪接口的离子迁移谱仪。

公开(公告)号：US07541576B2

公开(公告)日：2009-06-02

申请号：US11701752

申请日：2007-02-01

申请人： Mikhail E. Belov ,  Richard D. Smith

发明人： Mikhail E. Belov ,  Richard D. Smith

IPC分类号： H01J49/40

CPC分类号： H01J49/0031 ,  G01N27/622

摘要： A method for analyzing analytes from a sample introduced into a Spectrometer by generating a pseudo random sequence of a modulation bins, organizing each modulation bin as a series of submodulation bins, thereby forming an extended pseudo random sequence of submodulation bins, releasing the analytes in a series of analyte packets into a Spectrometer, thereby generating an unknown original ion signal vector, detecting the analytes at a detector, and characterizing the sample using the plurality of analyte signal subvectors. The method is advantageously applied to an Ion Mobility Spectrometer, and an Ion Mobility Spectrometer interfaced with a Time of Flight Mass Spectrometer.

摘要翻译： 一种用于通过产生调制箱的伪随机序列来分析从引入到光谱仪中的样品的分析物的方法，将每个调制箱组织成一系列的子调制箱，由此形成扩频的伪随机序列的子调制箱，将分析物在 一系列分析物分组进入光谱仪，从而产生未知的原始离子信号矢量，检测检测器处的​​分析物，并使用多个分析物信号子矢量表征样品。 该方法有利地应用于离子移动光谱仪和与飞行时间质谱仪接口的离子迁移光谱仪。

公开(公告)号：US09269548B2

公开(公告)日：2016-02-23

申请号：US13086244

申请日：2011-04-13

申请人： Mikhail E. Belov

发明人： Mikhail E. Belov

IPC分类号： H01J49/00 ,  H01J49/06 ,  G01N27/62

CPC分类号： H01J49/0031 ,  G01N27/622 ,  H01J49/061

摘要： An apparatus and method for analyzing a sample containing multiple analytes that combines a separation device that separates the individual analytes by virtue of some physical and/or chemical characteristic other than the mass to charge ratio (m/z) interfaced with a mass spectrometer that detects the m/z of individual analytes. Separation is performed on the shorter timescale than signal detection with the mass spectrometer. A preferred embodiment utilizes an ion mobility spectrometer interfaced with an Orbitrap mass spectrometer.

摘要翻译： 一种用于分析含有多个分析物的样品的装置和方法，所述样品组合了分离装置，所述分离装置通过除了检测到的质谱与质量比（m / z）之外的一些物理和/或化学特性分离各个分析物 单个分析物的m / z。 在比质谱仪的信号检测更短的时间刻度上进行分离。 优选的实施方案利用与Orbitrap质谱仪接口的离子迁移谱仪。