利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

25 条记录 (耗时 0.038 秒)

    Oligomeric compounds for the modulation of Bcl-2
    1.
    发明授权
    Oligomeric compounds for the modulation of Bcl-2 失效
    用于调节Bcl-2的低聚物

    公开(公告)号：US07622453B2

    公开(公告)日：2009-11-24

    申请号：US11021729

    申请日：2004-12-23

    申请人： Miriam Frieden ,  Jens B. Hansen ,  Henrik Orum ,  Majken Westergaard ,  Charlotte A. Thrue

    发明人： Miriam Frieden ,  Jens B. Hansen ,  Henrik Orum ,  Majken Westergaard ,  Charlotte A. Thrue

    IPC分类号： A01N61/00 ,  A01N43/04 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12N15/1135 ,  A61K48/00 ,  C07H21/00 ,  C12N2310/315 ,  C12N2310/3231

    摘要： The present invention provides improved oligomeric compound, in particular oligonucleotide compounds, and methods for modulating the expression of the Bcl-2 gene in humans. In particular, this invention relates to oligomeric compounds of 10-30 nucleobases in length which comprise a target binding domain that is specifically hybridizable to a region ranging from base position No. 1459 (5′) to No. 1476 (3′) of the human Bcl-2 mRNA, said target binding domain having the formula: 5′-[(DNA/RNA)0-1-(LNA/LNA*)2-7-(DNA/RNA/LNA*)4-14-(LNA/LNA*)2-7-(DNA/RNA)0-1]-3′ and said target binding domain comprising at least two LNA nucleotides or LNA analogue nucleotides linked by a phosphorothioate group (—O—P(O,S)—O—). In particular the oligo is predominantly or fully thiolated. The invention also provides the use of such oligomers or conjugates or chimera for the treatment of various diseases associated with the expression of the Bcl-2 gene, such as cancer.

    摘要翻译： 本发明提供了改进的低聚化合物，特别是寡核苷酸化合物，以及调节人类Bcl-2基因表达的方法。 特别地，本发明涉及长度为10-30个核碱基的寡聚化合物，其包含与从第1459（5'）至第1476（3'）的基础位置区别特异性杂交的靶结合结构域 所述靶结合结构域具有下式：5' - [（DNA / RNA）0-1-（LNA / LNA *）2-7-（DNA / RNA / LNA *）4-14-（ LNA / LNA *）2-7-（DNA / RNA）0-1] -3'，并且所述靶结合结构域包含由硫代磷酸酯基团连接的至少两个LNA核苷酸或LNA类似物核苷酸（-OP（O，S） - O-）。 特别地，寡核苷酸主要或完全硫醇化。 本发明还提供了这种寡聚物或缀合物或嵌合体用于治疗与Bcl-2基因（例如癌症）的表达相关的各种疾病的用途。

    Oligomeric compounds for the modulation of Bcl-2
    2.
    发明申请
    Oligomeric compounds for the modulation of Bcl-2 失效
    用于调节Bcl-2的低聚物

    公开(公告)号：US20050203042A1

    公开(公告)日：2005-09-15

    申请号：US11021729

    申请日：2004-12-23

    申请人： Miriam Frieden ,  Jens Hansen ,  Henrik Orum ,  Majken Westergaard ,  Charlotte Thrue

    发明人： Miriam Frieden ,  Jens Hansen ,  Henrik Orum ,  Majken Westergaard ,  Charlotte Thrue

    IPC分类号： A61K48/00 ,  C07H21/02 ,  C12N15/113

    CPC分类号： C12N15/1135 ,  A61K48/00 ,  C07H21/00 ,  C12N2310/315 ,  C12N2310/3231

    摘要： The present invention provides improved oligomeric compound, in particular oligonucleotide compounds, and methods for modulating the expression of the Bcl-2 gene in humans. In particular, this invention relates to oligomeric compounds of 10-30 nucleobases in length which comprise a target binding domain that is specifically hybridizable to a region ranging from base position No. 1459 (5′) to No. 1476 (3′) of the human Bcl-2 mRNA, said target binding domain having the formula: 5′-[(DNA/RNA)0-1-(LNA/LNA*)2-7-(DNA/RNA/LNA*)4-14-(LNA/LNA*)2-7-(DNA/RNA)0-1]-3 and said target binding domain comprising at least two LNA nucleotides or LNA analogue nucleotides linked by a phosphorothioate group (—O—P(O,S)—O—). In particular the oligo is predominantly or fully thiolated. The invention also provides the use of such oligomers or conjugates or chimera for the treatment of various diseases associated with the expression of the Bcl-2 gene, such as cancer.

    摘要翻译： 本发明提供了改进的低聚化合物，特别是寡核苷酸化合物，以及调节人类Bcl-2基因表达的方法。 特别地，本发明涉及长度为10-30个核碱基的寡聚化合物，其包含与从第1459（5'）至第1476（3'）的基础位置区别特异性杂交的靶结合结构域 所述目标结合结构域具有下式：5' - [（DNA / RNA）0-1 - （LNA / LNA *）2-7 - （DNA / RNA / LNA *）4-14 - （LNA / LNA *）2-7 - （DNA / RNA）

    POTENT LNA OLIGONUCLEOTIDES FOR THE INHIBITION OF HIF-1A EXPRESSION
    3.
    发明申请
    POTENT LNA OLIGONUCLEOTIDES FOR THE INHIBITION OF HIF-1A EXPRESSION 有权
    用于抑制HIF-1A表达的POTENT LNA寡核苷酸

    公开(公告)号：US20110183931A1

    公开(公告)日：2011-07-28

    申请号：US13079525

    申请日：2011-04-04

    申请人： Majken Westergaard ,  Charlotte A. Thrue ,  Frank W. Rasmussen ,  Henrik F. Hansen

    发明人： Majken Westergaard ,  Charlotte A. Thrue ,  Frank W. Rasmussen ,  Henrik F. Hansen

    IPC分类号： A61K31/7052 ,  A61K31/704 ,  A61P35/00

    CPC分类号： C12N15/113 ,  C12N2310/11 ,  C12N2310/315 ,  C12N2310/3231 ,  C12N2310/3341 ,  C12N2310/341 ,  C12N2310/345 ,  C12N2310/346

    摘要： The present disclosure relates to an LNA oligonucleotide consisting of a sequence selected from the group consisting of 5′-(Tx)GxGxcsasasgscsastscscsTxGxT-3′ and 5′-(Gx)TxascstsgscscststscsTxTxA-3′, wherein capital letters designate a beta-D-oxy-LNA nucleotide analogue, small letters designate a 2-deoxynucleotide, underline designates either a beta-D-oxy-LNA nucleotide analogue or a 2-deoxynucleotide, subscript “s” designates a phosphorothioate link between neighbouring nucleotides/LNA nucleotide analogues, and subscript “x” designates either a phosphorothioate link or a phosphorodiester link between neighbouring nucleotides/LNA nucleotide analogues, and wherein the sequence is optionally extended by up to five 2-deoxynucleotide units. The LNA oligonucleotides are useful for modulating the expression of hypoxia-inducible factor-1a (HIF-1a), e.g. in the treatment of cancer diseases, inhibiting angiogenesis, inducing apoptosis, preventing cellular proliferation, or treating an angiogenic disease, e.g. diabetic retinopathy, macular degeneration (ARMD), psoriasis, rheumatoid arthritis and other inflammatory diseases.

    摘要翻译： 本公开内容涉及由选自5' - （Tx）GxGxcsasasgscsastscscsTxGxT-3'和5' - （Gx）TxascstsgscscststscscTxTxA-3'的序列组成的LNA寡核苷酸，其中大写字母表示β-D-氧基 -LNA核苷酸类似物，小写字母表示2-脱氧核苷酸，下划线表示β-D-氧-LNA核苷酸类似物或2-脱氧核苷酸，下标“s”表示相邻核苷酸/ LNA核苷酸类似物之间的硫代磷酸酯连接，下标 “x”表示相邻核苷酸/ LNA核苷酸类似物之间的硫代磷酸酯连接或磷酸二酯连接，并且其中该序列任选地延伸多达五个2-脱氧核苷酸单元。 LNA寡核苷酸可用于调节缺氧诱导因子-1a（HIF-1a）的表达，例如， 在治疗癌症疾病，抑制血管发生，诱导细胞凋亡，预防细胞增殖或治疗血管生成疾病，例如， 糖尿病性视网膜病变，黄斑变性（ARMD），牛皮癣，类风湿性关节炎等炎症性疾病。

    POTENT LNA OLIGONUCLEOTIDES FOR THE INHIBITION OF HIF-1a EXPRESSION
    4.
    发明申请
    POTENT LNA OLIGONUCLEOTIDES FOR THE INHIBITION OF HIF-1a EXPRESSION 有权
    用于抑制HIF-1a表达的POTENT LNA寡核苷酸

    公开(公告)号：US20090286859A1

    公开(公告)日：2009-11-19

    申请号：US12477261

    申请日：2009-06-03

    申请人： Majken Westergaard ,  Charlotte A. Thrue ,  Frank W. Rasmussen ,  Henrik F. Hansen

    发明人： Majken Westergaard ,  Charlotte A. Thrue ,  Frank W. Rasmussen ,  Henrik F. Hansen

    IPC分类号： A61K31/7088 ,  A61P35/00

    CPC分类号： C12N15/113 ,  C12N2310/11 ,  C12N2310/315 ,  C12N2310/3231 ,  C12N2310/3341 ,  C12N2310/341 ,  C12N2310/345 ,  C12N2310/346

    摘要： The present disclosure relates to an LNA oligonucleotide consisting of a sequence selected from the group consisting of 5′-(Tx)GxGxcsasasgscsastscscsTxGxT-3′ and 5-(Gx)TxTxascstsgscscststscsTxTxA-3′, wherein capital letters designate a beta-D-oxy-LNA nucleotide analogue, small letters designate a 2-deoxynucleotide, underline designates either a beta-D-oxy-LNA nucleotide analogue or a 2-deoxynucleotide, subscript “s” designates a phosphorothioate link between neighbouring nucleotides/LNA nucleotide analogues, and subscript “x” designates either a phosphorothioate link or a phosphorodiester link between neighbouring nucleotides/LNA nucleotide analogues, and wherein the sequence is optionally extended by up to five 2-deoxynucleotide units. The LNA oligonucleotides are useful for modulating the expression of hypoxia-inducible factor-1a (HIF-1a), e.g. in the treatment of cancer diseases, inhibiting angiogenesis, inducing apoptosis, preventing cellular proliferation, or treating an angiogenic disease, e.g. diabetic retinopathy, macular degeneration (ARMD), psoriasis, rheumatoid arthritis and other inflammatory diseases.

    摘要翻译： 本发明涉及由选自5' - （Tx）GxGxcsasasgscsastscscsTxGxT-3'和5-（Gx）TxTxascstsgscscststscscTTTTXA-3'的序列组成的LNA寡核苷酸，其中大写字母表示β-D-氧 - LNA核苷酸类似物，小写字母表示2-脱氧核苷酸，下划线表示β-D-氧-LNA核苷酸类似物或2-脱氧核苷酸，下标“s”表示相邻核苷酸/ LNA核苷酸类似物之间的硫代磷酸酯连接，下标“ x“表示相邻核苷酸/ LNA核苷酸类似物之间的硫代磷酸酯连接或磷酸二酯连接，并且其中该序列任选地延伸多达五个2-脱氧核苷酸单元。 LNA寡核苷酸可用于调节缺氧诱导因子-1a（HIF-1a）的表达，例如， 在治疗癌症疾病，抑制血管发生，诱导细胞凋亡，预防细胞增殖或治疗血管生成疾病，例如， 糖尿病性视网膜病变，黄斑变性（ARMD），牛皮癣，类风湿性关节炎等炎症性疾病。

    Potent LNA oligonucleotides for the inhibition of HIF-1A expression
    5.
    发明授权
    Potent LNA oligonucleotides for the inhibition of HIF-1A expression 有权
    用于抑制HIF-1A表达的强效LNA寡核苷酸

    公开(公告)号：US07589190B2

    公开(公告)日：2009-09-15

    申请号：US11271686

    申请日：2005-11-09

    申请人： Majken Westergaard ,  Charlotte A. Thrue ,  Frank W. Rasmussen ,  Henrik F. Hansen

    发明人： Majken Westergaard ,  Charlotte A. Thrue ,  Frank W. Rasmussen ,  Henrik F. Hansen

    IPC分类号： C07H21/02 ,  C07H21/04 ,  C12Q1/68 ,  A01N61/00 ,  A01N43/04

    CPC分类号： C12N15/113 ,  C12N2310/11 ,  C12N2310/315 ,  C12N2310/3231 ,  C12N2310/3341 ,  C12N2310/341 ,  C12N2310/345 ,  C12N2310/346

    摘要： The present disclosure relates to an LNA oligonucleotide consisting of a sequence selected from the group consisting of 5′-(Tx)GxGxcsasasgscsastscscsTxGxT-3′ and 5′-(Gx)TxTxascstsgscscststscsTxTxA-3′, wherein capital letters designate a beta-D-oxy-LNA nucleotide analogue, small letters designate a 2-deoxynucleotide, underline designates either a beta-D-oxy-LNA nucleotide analogue or a 2-deoxynucleotide, subscript “s” designates a phosphorothioate link between neighbouring nucleotides/LNA nucleotide analogues, and subscript “x” designates either a phosphorothioate link or a phosphorodiester link between neighbouring nucleotides/LNA nucleotide analogues, and wherein the sequence is optionally extended by up to five 2-deoxynucleotide units. The LNA oligonucleotides are useful for modulating the expression of hypoxia-inducible factor-1a (HIF-1a), e.g. in the treatment of cancer diseases, inhibiting angiogenesis, inducing apoptosis, preventing cellular proliferation, or treating an angiogenic disease, e.g. diabetic retinopathy, macular degeneration (ARMD), psoriasis, rheumatoid arthritis and other inflammatory diseases.

    摘要翻译： 本发明涉及由选自5' - （Tx）GxGxcsasasgscsastscscs TxGxT-3'和5' - （ Gx）TxTxascstsgscscststscsTxTxA-3'，其中大写字母表示β-D-氧-LNA核苷酸类似物，小字母表示2-脱氧核苷酸，下划线指定 β-D-氧-LNA核苷酸类似物或2-脱氧核苷酸，下标“s”表示相邻核苷酸/ LNA核苷酸类似物之间的硫代磷酸酯连接，下标“x”表示硫代磷酸酯连接或相邻核苷酸/ LNA核苷酸类似物，并且其中所述序列任选地延伸多达五个2-脱氧核苷酸单元。 LNA寡核苷酸可用于调节缺氧诱导因子-1a（HIF-1a）的表达，例如， 在治疗癌症疾病，抑制血管发生，诱导细胞凋亡，预防细胞增殖或治疗血管生成疾病，例如， 糖尿病性视网膜病变，黄斑变性（ARMD），牛皮癣，类风湿性关节炎等炎症性疾病。

    LNA OLIGONUCLEOTIDES AND THE TREATMENT OF CANCER
    10.
    发明申请
    LNA OLIGONUCLEOTIDES AND THE TREATMENT OF CANCER 审中-公开
    LNA OLIGONUCLEOTIDES AND THE ACCEATMENT OF CANCER

    公开(公告)号：US20120322848A1

    公开(公告)日：2012-12-20

    申请号：US13443557

    申请日：2012-04-10

    申请人： Christoph Rosenbohm ,  Lene S. Kjaerulff ,  Majken Westergaard ,  Margit Wissenbach ,  Jens B. Hansen ,  Marlene Asklund

    发明人： Christoph Rosenbohm ,  Lene S. Kjaerulff ,  Majken Westergaard ,  Margit Wissenbach ,  Jens B. Hansen ,  Marlene Asklund

    IPC分类号： A61K31/712 ,  A61P35/02 ,  A61P35/00

    CPC分类号： C12N15/1135 ,  A61K38/00 ,  C12N2310/11 ,  C12N2310/315 ,  C12N2310/319 ,  C12N2310/3341

    摘要： The present disclosure concerns LNA oligonucleotides having a (sub)sequence of the general formula 5′-(MeCx)(Tx)MeCxAsAstsCsCsastsgsgsMeCxAx(Gx)(c)-3′, and preferably of the general formula 5′-MeCxTxMeCxAsastscscsastsgsgsMeCxAxGxc-3′, wherein capital letters designate an LNA nucleotide analogue selected from β-D-oxy-LNA, β-D-thio-LNA, β-D-amino-LNA and α-L-oxy-LNA, small letters designate a deoxynucleotide, and underline designates either an LNA nucleotide analogue as defined above or a deoxynucleotide. Such LNA oligonucleotides exhibit surprisingly good properties with respect to inhibition of the expression of Survivin by means of an anti-sense mechanism, and thereby lead to reduction or inhibition of tumour development in vivo. The LNA oligonucleotides are superior to other LNA oligonucletides targeting Surviving mRNA measured by functional read outs such as apoptosis induction and proliferation inhibition, and is potent in down-regulating Survivin mRNA and protein in transfected cancer cell lines, and induce apoptosis in combination with Taxol superior compared to other LNA oligonucleotides.

    摘要翻译： 本公开内容涉及具有通式5'（（MeCx）（Tx）MeCxAsAstsCsCsastsgsgsMeCxAx（Gx）（c）-3'，优选通式为5'-MeCxTxMeCxAsastscscsastsgsgsMeCxAxGxc-3'的（亚）序列的LNA寡核苷酸， 其中大写字母表示选自以下的LNA核苷酸类似物：-D-oxy-LNA，＆bgr; -D-thio-LNA，＆bgr; -D-氨基-LNA和α-L-氧-LNA，小字母表示脱氧核苷酸 ，下划线表示如上定义的LNA核苷酸类似物或脱氧核苷酸。 这样的LNA寡核苷酸在通过反义机制抑制存活蛋白的表现方面表现出惊人的良好性质，从而导致体内肿瘤发展的减少或抑制。 LNA寡核苷酸优于靶向通过功能读数（如凋亡诱导和增殖抑制）测量的存活mRNA的其他LNA寡核苷酸，并且在转染的癌细胞系中下调Survivin mRNA和蛋白质，并且与Taxol优异结合诱导凋亡有效 与其他LNA寡核苷酸相比。