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公开(公告)号:US07824676B2
公开(公告)日:2010-11-02
申请号:US11522043
申请日:2006-09-15
申请人: Miyuki Nishimura , Mayumi Asano , Yuichi Ono , Koji Morimoto , Masakazu Takeuchi , Yoko Inoue , Toshio Imai , Yoshimi Takai
发明人: Miyuki Nishimura , Mayumi Asano , Yuichi Ono , Koji Morimoto , Masakazu Takeuchi , Yoko Inoue , Toshio Imai , Yoshimi Takai
IPC分类号: C07K16/18
CPC分类号: C07K14/47 , C07K14/705
摘要: A mouse cDNA library from gene fragments encoding proteins localizing at cell-cell junctions was screened by a technique visualizing localization of a protein to identify a junction-enriched and -associated protein, JEAP. GenBank homology search was performed based on the sequence. Based on the obtained sequence, a mouse cDNA library was screened to identify JEAP-2. By using prepared antibodies against these proteins, it was revealed that these proteins express specifically at tight junctions, in particular, tight junctions in exocrine glands.
摘要翻译: 通过技术可视化蛋白质的定位技术筛选编码在细胞连接处定位的蛋白质的蛋白质的基因片段的小鼠cDNA文库,以鉴定连接蛋白和相关蛋白JEAP。 GenBank同源性检索是根据序列进行的。 基于获得的序列,筛选小鼠cDNA文库以鉴定JEAP-2。 通过使用针对这些蛋白质的制备的抗体,显示这些蛋白质在紧密连接处特别是在外分泌腺中的紧密连接处特异性表达。
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公开(公告)号:US07129063B2
公开(公告)日:2006-10-31
申请号:US10298417
申请日:2002-11-15
申请人: Miyuki Nishimura , Mayumi Asano , Yuichi Ono , Koji Morimoto , Masakazu Takeuchi , Yoko Inoue , Toshio Imai , Yoshimi Takai
发明人: Miyuki Nishimura , Mayumi Asano , Yuichi Ono , Koji Morimoto , Masakazu Takeuchi , Yoko Inoue , Toshio Imai , Yoshimi Takai
CPC分类号: C07K14/47 , C07K14/705
摘要: A mouse cDNA library from gene fragments encoding proteins localizing at cell-cell junctions was screened by a technique visualizing localization of a protein to to identify a junction-enriched and -associated protein, JEAP. GenBank homology search was performed based on the sequence. Based on the obtained sequence, a mouse cDNA library was screened to identify JEAP-2. By using prepared antibodies against these proteins, it was revealed that these proteins express specifically at tight junctions, in particular, tight junctions in exocrine glands.
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公开(公告)号:US20080119641A1
公开(公告)日:2008-05-22
申请号:US11522043
申请日:2006-09-15
申请人: Miyuki Nishimura , Mayumi Asano , Yuichi Ono , Koji Morimoto , Masakazu Takeuchi , Yoko Inoue , Toshio Imai , Yoshimi Takai
发明人: Miyuki Nishimura , Mayumi Asano , Yuichi Ono , Koji Morimoto , Masakazu Takeuchi , Yoko Inoue , Toshio Imai , Yoshimi Takai
IPC分类号: C07K16/18
CPC分类号: C07K14/47 , C07K14/705
摘要: A mouse cDNA library from gene fragments encoding proteins localizing at cell—cell junctions was screened by a technique visualizing localization of a protein to identify a junction-enriched and -associated protein, JEAP. GenBank homology search was performed based on the sequence. Based on the obtained sequence, a mouse cDNA library was screened to identify JEAP-2. By using prepared antibodies against these proteins, it was revealed that these proteins express specifically at tight junctions, in particular, tight junctions in exocrine glands.
摘要翻译: 通过技术可视化蛋白质的定位技术筛选编码在细胞连接处定位的蛋白质的蛋白质的基因片段的小鼠cDNA文库,以鉴定连接蛋白和相关蛋白JEAP。 GenBank同源性检索是根据序列进行的。 基于获得的序列,筛选小鼠cDNA文库以鉴定JEAP-2。 通过使用针对这些蛋白质的制备的抗体,显示这些蛋白质在紧密连接处特别是在外分泌腺中的紧密连接处特异性表达。
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