公开(公告)号：US07824676B2

公开(公告)日：2010-11-02

申请号：US11522043

申请日：2006-09-15

申请人： Miyuki Nishimura ,  Mayumi Asano ,  Yuichi Ono ,  Koji Morimoto ,  Masakazu Takeuchi ,  Yoko Inoue ,  Toshio Imai ,  Yoshimi Takai

发明人： Miyuki Nishimura ,  Mayumi Asano ,  Yuichi Ono ,  Koji Morimoto ,  Masakazu Takeuchi ,  Yoko Inoue ,  Toshio Imai ,  Yoshimi Takai

IPC分类号： C07K16/18

CPC分类号： C07K14/47 ,  C07K14/705

摘要： A mouse cDNA library from gene fragments encoding proteins localizing at cell-cell junctions was screened by a technique visualizing localization of a protein to identify a junction-enriched and -associated protein, JEAP. GenBank homology search was performed based on the sequence. Based on the obtained sequence, a mouse cDNA library was screened to identify JEAP-2. By using prepared antibodies against these proteins, it was revealed that these proteins express specifically at tight junctions, in particular, tight junctions in exocrine glands.

摘要翻译： 通过技术可视化蛋白质的定位技术筛选编码在细胞连接处定位的蛋白质的蛋白质的基因片段的小鼠cDNA文库，以鉴定连接蛋白和相关蛋白JEAP。 GenBank同源性检索是根据序列进行的。 基于获得的序列，筛选小鼠cDNA文库以鉴定JEAP-2。 通过使用针对这些蛋白质的制备的抗体，显示这些蛋白质在紧密连接处特别是在外分泌腺中的紧密连接处特异性表达。

公开(公告)号：US07129063B2

公开(公告)日：2006-10-31

申请号：US10298417

申请日：2002-11-15

申请人： Miyuki Nishimura ,  Mayumi Asano ,  Yuichi Ono ,  Koji Morimoto ,  Masakazu Takeuchi ,  Yoko Inoue ,  Toshio Imai ,  Yoshimi Takai

发明人： Miyuki Nishimura ,  Mayumi Asano ,  Yuichi Ono ,  Koji Morimoto ,  Masakazu Takeuchi ,  Yoko Inoue ,  Toshio Imai ,  Yoshimi Takai

IPC分类号： C12P21/02 ,  C12N15/63 ,  C12N1/21 ,  C12N1/19 ,  C12N5/10 ,  C07H21/04

CPC分类号： C07K14/47 ,  C07K14/705

摘要： A mouse cDNA library from gene fragments encoding proteins localizing at cell-cell junctions was screened by a technique visualizing localization of a protein to to identify a junction-enriched and -associated protein, JEAP. GenBank homology search was performed based on the sequence. Based on the obtained sequence, a mouse cDNA library was screened to identify JEAP-2. By using prepared antibodies against these proteins, it was revealed that these proteins express specifically at tight junctions, in particular, tight junctions in exocrine glands.

公开(公告)号：US20080119641A1

公开(公告)日：2008-05-22

申请号：US11522043

申请日：2006-09-15

申请人： Miyuki Nishimura ,  Mayumi Asano ,  Yuichi Ono ,  Koji Morimoto ,  Masakazu Takeuchi ,  Yoko Inoue ,  Toshio Imai ,  Yoshimi Takai

发明人： Miyuki Nishimura ,  Mayumi Asano ,  Yuichi Ono ,  Koji Morimoto ,  Masakazu Takeuchi ,  Yoko Inoue ,  Toshio Imai ,  Yoshimi Takai

IPC分类号： C07K16/18

CPC分类号： C07K14/47 ,  C07K14/705

摘要： A mouse cDNA library from gene fragments encoding proteins localizing at cell—cell junctions was screened by a technique visualizing localization of a protein to identify a junction-enriched and -associated protein, JEAP. GenBank homology search was performed based on the sequence. Based on the obtained sequence, a mouse cDNA library was screened to identify JEAP-2. By using prepared antibodies against these proteins, it was revealed that these proteins express specifically at tight junctions, in particular, tight junctions in exocrine glands.

摘要翻译： 通过技术可视化蛋白质的定位技术筛选编码在细胞连接处定位的蛋白质的蛋白质的基因片段的小鼠cDNA文库，以鉴定连接蛋白和相关蛋白JEAP。 GenBank同源性检索是根据序列进行的。 基于获得的序列，筛选小鼠cDNA文库以鉴定JEAP-2。 通过使用针对这些蛋白质的制备的抗体，显示这些蛋白质在紧密连接处特别是在外分泌腺中的紧密连接处特异性表达。

公开(公告)号：US07622270B2

公开(公告)日：2009-11-24

申请号：US10532264

申请日：2003-10-21

申请人： Yasuko Nakagawa ,  Yuichi Ono ,  Yoshimasa Sakamoto ,  Eri Mizuhara ,  Tomoya Nakatani ,  Yoshimi Takai

发明人： Yasuko Nakagawa ,  Yuichi Ono ,  Yoshimasa Sakamoto ,  Eri Mizuhara ,  Tomoya Nakatani ,  Yoshimi Takai

IPC分类号： G01N33/567 ,  C07K16/28

CPC分类号： G01N33/6896 ,  C07K14/47

摘要： A novel gene 65B13 expressed specifically and transiently in dopaminergic neuron precursor cells immediately after cell cycle exit was obtained by the present invention. The cellular expression of 65B13 can be used as an index to select cells that are suitable in terms of their safety, survival rate, and network formation ability, for transplant therapy of neurodegenerative diseases such as Parkinson's disease.

摘要翻译： 通过本发明获得在细胞周期出口后立即在多巴胺能神经元前体细胞中特异性和瞬时表达的新基因65B13。 65B13的细胞表达可用作选择适合于其安全性，存活率和网络形成能力的细胞的指标，用于神经变性疾病如帕金森病的移植治疗。

公开(公告)号：US07153831B2

公开(公告)日：2006-12-26

申请号：US11257883

申请日：2005-10-25

申请人： Masakazu Takeuchi ,  Yoshimi Takai

发明人： Masakazu Takeuchi ,  Yoshimi Takai

IPC分类号： A61K38/00

CPC分类号： C07K16/18 ,  C07K14/4702 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5023 ,  G01N33/68 ,  G01N2500/00

摘要： A protein useful for clarifying the regulation mechanism of Ca2+-dependent exocytosis in particular, the activation and inactivation of Rab3A, and a method of screening for a material useful for regulating Ca2+-dependent exocytosis in particular, the activation and inactivation of Rab3A, using the above protein. By using the coimmunoprecipitation with an anti-Rab3A GEP antibody, a protein participated in the regulation of activation or inactivation of Rab3A is determined. As the protein binds to rabconnectin-3 and GDP/GTP exchange protein, it can be used for screening for a material that increases or decreases the binding.

公开(公告)号：US20060246502A1

公开(公告)日：2006-11-02

申请号：US11483356

申请日：2006-07-07

申请人： Masakazu Takeuchi ,  Yoshimi Takai

发明人： Masakazu Takeuchi ,  Yoshimi Takai

IPC分类号： C12Q1/68 ,  G01N33/567 ,  C07H21/04 ,  C12P21/06 ,  C07K14/705 ,  C07K16/28

CPC分类号： C07K16/18 ,  C07K14/4702 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5023 ,  G01N33/68 ,  G01N2500/00

摘要： A protein useful for clarifying the regulation mechanism of Ca2+-dependent exocytosis in particular, the activation and inactivation of Rab3A, and a method of screening for a material useful for regulating Ca2+-dependent exocytosis in particular, the activation and inactivation of Rab3A, using the above protein. By using the coimmunoprecipitation with an anti-Rab3A GEP antibody, a protein participated in the regulation of activation or inactivation of Rab3A is determined. As the protein binds to rabconnectin-3 and GDP/GTP exchange protein, it can be used for screening for a material that increases or decreases the binding.

摘要翻译： 可用于澄清Ca 2+依赖性胞吐作用的调节机制的蛋白质，特别是Rab3A的活化和失活，以及筛选用于调节Ca 2+， / SUP>依赖性胞吐，特别是使用上述蛋白质激活和失活Rab3A。 通过使用抗Rab3A GEP抗体的共免疫沉淀，测定参与Rab3A的激活或失活调节的蛋白质。 当蛋白质与rabconnectin-3和GDP / GTP交换蛋白结合时，它可用于筛选增加或减少结合的材料。

公开(公告)号：US20070122882A1

公开(公告)日：2007-05-31

申请号：US11622312

申请日：2007-01-11

申请人： Yasuko Nakagawa ,  Yuichi Ono ,  Yoshimasa Sakamoto ,  Eri Mizuhara ,  Tomoya Nakatani ,  Yoshimi Takai

发明人： Yasuko Nakagawa ,  Yuichi Ono ,  Yoshimasa Sakamoto ,  Eri Mizuhara ,  Tomoya Nakatani ,  Yoshimi Takai

IPC分类号： C07K14/705 ,  C07K16/28 ,  C07H21/04 ,  C12P21/06

CPC分类号： G01N33/6896 ,  C07K14/47

摘要： A novel gene 65B13 expressed specifically and transiently in dopaminergic neuron precursor cells immediately after cell cycle exit was obtained by the present invention. The cellular expression of 65B13 can be used as an index to select cells that are suitable in terms of their safety, survival rate, and network formation ability, for transplant therapy of neurodegenerative diseases such as Parkinson's disease.

摘要翻译： 通过本发明获得在细胞周期出口后立即在多巴胺能神经元前体细胞中特异性和瞬时表达的新基因65B13。 65B13的细胞表达可用作选择适合于其安全性，存活率和网络形成能力的细胞的指标，用于神经变性疾病如帕金森病的移植治疗。

公开(公告)号：US20060239978A1

公开(公告)日：2006-10-26

申请号：US10532264

申请日：2003-10-21

申请人： Yasuko Nakagawa ,  Yuichi Ono ,  Yoshima Sakamoto ,  Eri Mizuhara ,  Tomoya Nakatani ,  Yoshimi Takai

发明人： Yasuko Nakagawa ,  Yuichi Ono ,  Yoshima Sakamoto ,  Eri Mizuhara ,  Tomoya Nakatani ,  Yoshimi Takai

IPC分类号： A61K35/30 ,  C12Q1/68 ,  G01N33/567 ,  C07H21/04 ,  C12N5/08 ,  C07K14/705 ,  C07K16/28

CPC分类号： G01N33/6896 ,  C07K14/47

摘要： A novel gene 65B13 expressed specifically and transiently in dopaminergic neuron precursor cells immediately after cell cycle exit was obtained by the present invention. The cellular expression of 65B13 can be used as an index to select cells that are suitable in terms of their safety, survival rate, and network formation ability, for transplant therapy of neurodegenerative diseases such as Parkinson's disease.

摘要翻译： 通过本发明获得在细胞周期出口后立即在多巴胺能神经元前体细胞中特异性和瞬时表达的新基因65B13。 65B13的细胞表达可用作选择适合于其安全性，存活率和网络形成能力的细胞的指标，用于神经变性疾病如帕金森病的移植治疗。

公开(公告)号：US07279461B2

公开(公告)日：2007-10-09

申请号：US11483356

申请日：2006-07-07

申请人： Masakazu Takeuchi ,  Yoshimi Takai

发明人： Masakazu Takeuchi ,  Yoshimi Takai

IPC分类号： A61K38/00

CPC分类号： C07K16/18 ,  C07K14/4702 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5023 ,  G01N33/68 ,  G01N2500/00

摘要： A protein useful for clarifying the regulation mechanism of Ca2+-dependent exocytosis in particular, the activation and inactivation of Rab3A, and a method of screening for a material useful for regulating Ca2+-dependent exocytosis in particular, the activation and inactivation of Rab3A, using the above protein. By using the coimmunoprecipitation with an anti-Rab3A GEP antibody, a protein participated in the regulation of activation or inactivation of Rab3A is determined. As the protein binds to rabconnectin-3 and GDP/GTP exchange protein, it can be used for screening for a material that increases or decreases the binding.

摘要翻译： 可用于澄清Ca 2+依赖性胞吐作用的调节机制的蛋白质，特别是Rab3A的活化和失活，以及筛选用于调节Ca 2+， / SUP>依赖性胞吐，特别是使用上述蛋白质激活和失活Rab3A。 通过使用抗Rab3A GEP抗体的共免疫沉淀，测定参与Rab3A的激活或失活调节的蛋白质。 当蛋白质与rabconnectin-3和GDP / GTP交换蛋白结合时，它可用于筛选增加或减少结合的材料。

公开(公告)号：US20060040328A1

公开(公告)日：2006-02-23

申请号：US11257883

申请日：2005-10-25

申请人： Masakazu Takeuchi ,  Yoshimi Takai

发明人： Masakazu Takeuchi ,  Yoshimi Takai

IPC分类号： G01N33/53 ,  C07H21/04 ,  C12P21/06 ,  C07K14/705

CPC分类号： C07K16/18 ,  C07K14/4702 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5023 ,  G01N33/68 ,  G01N2500/00

摘要： A protein useful for clarifying the regulation mechanism of Ca2+-dependent exocytosis in particular, the activation and inactivation of Rab3A, and a method of screening for a material useful for regulating Ca2+-dependent exocytosis in particular, the activation and inactivation of Rab3A, using the above protein. By using the coimmunoprecipitation with an anti-Rab3A GEP antibody, a protein participated in the regulation of activation or inactivation of Rab3A is determined. As the protein binds to rabconnectin-3 and GDP/GTP exchange protein, it can be used for screening for a material that increases or decreases the binding.