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    Methods for transforming Phaffia strains, transformed Phaffia strains so obtained and recombinant DNA in said methods
    3.
    发明授权
    Methods for transforming Phaffia strains, transformed Phaffia strains so obtained and recombinant DNA in said methods 有权
    用于在所述方法中转化Phaffia菌株，如此获得的转化的Phaffia菌株和重组DNA的方法

    公开(公告)号：US06329141B1

    公开(公告)日：2001-12-11

    申请号：US09091725

    申请日：1998-11-19

    申请人： Albert Johannes Joseph Van Ooijen ,  Jan Cornelis Verdoes ,  Jan Wery

    发明人： Albert Johannes Joseph Van Ooijen ,  Jan Cornelis Verdoes ,  Jan Wery

    IPC分类号： C12Q168

    CPC分类号： C12P23/00 ,  C07K14/39 ,  C12N9/00 ,  C12N9/0004 ,  C12N9/0008 ,  C12N9/1085 ,  C12N9/90 ,  C12N15/52 ,  C12N15/815 ,  C12Y102/01012

    摘要： The present invention provides recombinant DNA comprising a transcription promoter and a downstream sequence to be expressed, in operable linkage therewith, wherein the transcription promoter comprises a region found upstream of the open reading frame of a highly expressed Phaffia gene, preferably a glycolytic pathway gene, more preferably the gene coding for Glyceraldehyde-3-Phosphate Dehydrogenase. Further preferred recombinant DNAs according to the invention contain promoters of ribosomal protein encoding genes, more preferably wherein the transcription promoter comprises a region found upstream of the open reading frame encoding a protein as represented by one of the disclosed amino acid sequences. According to a further aspect of the invention an isolated DNA sequence coding for an enzyme involved in the carotenoid biosynthetic pathway of Phaffia rhodozyma is provided.

    摘要翻译： 本发明提供了包含转录启动子和待表达的下游序列的重组DNA，其可操作地连接于其中，其中转录启动子包含在高度表达的菲菲亚基因，优选糖酵解途径基因的开放阅读框的上游发现的区域， 更优选为编码甘油醛-3-磷酸脱氢酶的基因。 更优选的根据本发明的重组DNA包含核糖体蛋白质编码基因的启动子，更优选地，其中转录启动子包含编码由所公开的氨基酸序列之一表示的蛋白质的开放阅读框上游的区域。 根据本发明的另一方面，提供了编码参与红发夫酵母的类胡萝卜素生物合成途径的酶的分离的DNA序列。

    Recombinant materials for carotenoid production
    4.
    发明授权
    Recombinant materials for carotenoid production 有权
    用于类胡萝卜素生产的重组材料

    公开(公告)号：US07205123B2

    公开(公告)日：2007-04-17

    申请号：US10001192

    申请日：2001-10-30

    申请人： Albert Johannes Joseph Van Ooijen ,  Jan Cornelis Verdoes ,  Jan Wery

    发明人： Albert Johannes Joseph Van Ooijen ,  Jan Cornelis Verdoes ,  Jan Wery

    IPC分类号： C12P23/00

    CPC分类号： C12P23/00 ,  C07K14/39 ,  C12N9/00 ,  C12N9/0004 ,  C12N9/0008 ,  C12N9/1085 ,  C12N9/90 ,  C12N15/52 ,  C12N15/815 ,  C12Y102/01012

    摘要： The present invention provides recombinant DNA comprising a transcription promoter and a downstream sequence to be expressed, in operable linkage therewith, wherein the transcription promoter comprises a region found upstream of the open reading frame of a highly expressed Phaffia gene, preferably a glycolytic pathway gene, more preferably the gene coding for Glyceraldehyde-3-Phosphate Dehydrogenase. Further preferred recombinant DNAs according to the invention contain promoters of ribosomal protein encoding genes, more preferably wherein the transcription promoter comprises a region found upstream of the open reading frame encoding a protein as represented by one of the amino acid sequences depicted in any one of SEQIDNOs: 24 to 50. According to a further aspect of the invention an isolated DNA sequence coding for an enzyme involved in the carotenoid biosynthetic pathway of Phaffia rhodozyma is provided, preferably wherein said enzyme has an activity selected from isopentenyl pyrophosphate isomerase activity, geranylgeranyl pyrophosphate synthase activity, phytoene synthase activity, phytoene desaturase activity and lycopene cyclase activity, still more preferably those coding for an enzyme having an amino acid sequence selected from the one represented by SEQIDNO: 13, SEQIDNO: 15, SEQIDNO: 17, SEQIDNO: 19, SEQIDNO: 21 or SEQIDNO: 23. Further embodiments concern vectors, transformed host organisms, methods for making proteins and/or carotenoids, such as astaxanthin, and methods for isolating highly expressed promoters from Phaffia.

    摘要翻译： 本发明提供了包含转录启动子和待表达的下游序列的重组DNA，其可操作地连接于其中，其中转录启动子包含在高度表达的菲菲亚基因，优选糖酵解途径基因的开放阅读框的上游发现的区域， 更优选为编码甘油醛-3-磷酸脱氢酶的基因。 更优选的根据本发明的重组DNA包含核糖体蛋白质编码基因的启动子，更优选地，其中转录启动子包含编码如SEQ ID NO中任一个所示的氨基酸序列之一的开放阅读框上游的区域 根据本发明的另一方面，提供了编码参与红发夫酵母的类胡萝卜素生物合成途径的酶的分离的DNA序列，优选其中所述酶具有选自异戊烯基焦磷酸异构酶活性，香叶基香叶基焦磷酸合酶 活性，八氢番茄红素合成酶活性，八氢番茄红素去饱和酶活性和番茄红素环化酶活性，还更优选编码具有选自SEQ ID NO：13，SEQ ID NO：15，SEQ ID NO：17，SEQ ID NO：19，SEQ ID NO ：21或SEQ ID NO：23.其他实施方案涉及载体，转化 宿主生物，用于制备蛋白质和/或类胡萝卜素的方法，例如虾青素，以及从Phaffia分离高表达启动子的方法。