公开(公告)号：US20240301435A1

公开(公告)日：2024-09-12

申请号：US18609293

申请日：2024-03-19

Applicant: Monsanto Technology LLC

Inventor： Justin Arsenault ,  Yurong Chen ,  Byung-Guk Kang ,  Jennifer Kumpf ,  Brian J. Martinell ,  Lorena B. Moeller ,  Mary Ann Saltarikos ,  Ashok Shrawat ,  Shubha Subbarao ,  Edward J. Williams ,  Xudong Ye

IPC: C12N15/82 ,  A01H4/00

CPC classification number: C12N15/8205 ,  A01H4/008

Abstract: The invention provides novel compositions and methods for improving the transformation of monocot seed excised embryo explants, which may include one or more steps of explant preparation, explant rehydration, Rhizobiales bacterium inoculation and co-culture, bud induction, extended bud induction, or regeneration of genetically modified plants or plant parts. The methods provided herein may include transforming at least one plant cell of the embryo explant with a heterologous polynucleotide by inoculating the embryo explant with a Rhizobiales bacterium comprising the heterologous polynucleotide. The methods provided herein also include methods of regenerating a genetically modified plant or plant part from a transformed or edited plant cell or explant.

公开(公告)号：US20240093222A1

公开(公告)日：2024-03-21

申请号：US18480381

申请日：2023-10-03

Applicant: Monsanto Technology LLC

Inventor： Yurong Chen ,  Brian J. Martinell ,  Anatoly Rivlin ,  Ashok Shrawat ,  Yuechun Wan ,  Edward J. Williams ,  Xudong Ye

IPC: C12N15/82

CPC classification number: C12N15/8275 ,  C12N15/8274

Abstract: The present invention provides methods for the production of viable explants from mature corn seeds, wherein the explant comprises the apical portion of the embryo axis of the corn seed. The present invention also relates to methods for producing such explants and for transforming the explants with a heterologous DNA.

公开(公告)号：US20220340925A1

公开(公告)日：2022-10-27

申请号：US13873092

申请日：2013-04-29

Applicant: Monsanto Technology LLC

Inventor： Yurong Chen ,  Brian J. Martinell ,  Anatoly Rivlin ,  Yuechun Wan ,  Edward J. Williams ,  Xudong Ye ,  Ashok Shrawat

IPC: C12N15/82

Abstract: The present invention provides methods for the production of viable explants from mature corn seeds, wherein the explant comprises the apical portion of the embryo axis of the corn seed. The present invention also relates to methods for producing such explants and for transforming the explants with a heterologous DNA.

公开(公告)号：US11713465B2

公开(公告)日：2023-08-01

申请号：US14550694

申请日：2014-11-21

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： David R. Duncan ,  Sarah Heifner ,  David Kelm ,  Brian J. Martinell ,  Lorena Moeller ,  Anatoly Rivlin ,  Rebecca Rode ,  Xudong Ye ,  Ashok Shrawat ,  Yurong Chen

IPC: C12N15/00 ,  C12N15/82

CPC classification number: C12N15/8205 ,  C12N15/8209

Abstract: The present invention provides methods for the transformation of viable explants from wheat seeds to permit production of transgenic wheat plants. The present invention also relates to methods for producing such explants and related embodiments.

公开(公告)号：US20220340916A1

公开(公告)日：2022-10-27

申请号：US14550694

申请日：2014-11-21

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： David R. Duncan ,  Sarah Heifner ,  David Kelm ,  Brian J. Martinell ,  Lorena Moeller ,  Anatoly Rivlin ,  Rebecca Rode ,  Xudong Ye ,  Ashok Shrawat ,  Yurong Chen

IPC: C12N15/82

Abstract: The present invention provides methods for the transformation of viable explants from wheat seeds to permit production of transgenic wheat plants. The present invention also relates to methods for producing such explants and related embodiments.