公开(公告)号：US20240301435A1

公开(公告)日：2024-09-12

申请号：US18609293

申请日：2024-03-19

Applicant: Monsanto Technology LLC

Inventor： Justin Arsenault ,  Yurong Chen ,  Byung-Guk Kang ,  Jennifer Kumpf ,  Brian J. Martinell ,  Lorena B. Moeller ,  Mary Ann Saltarikos ,  Ashok Shrawat ,  Shubha Subbarao ,  Edward J. Williams ,  Xudong Ye

IPC: C12N15/82 ,  A01H4/00

CPC classification number: C12N15/8205 ,  A01H4/008

Abstract: The invention provides novel compositions and methods for improving the transformation of monocot seed excised embryo explants, which may include one or more steps of explant preparation, explant rehydration, Rhizobiales bacterium inoculation and co-culture, bud induction, extended bud induction, or regeneration of genetically modified plants or plant parts. The methods provided herein may include transforming at least one plant cell of the embryo explant with a heterologous polynucleotide by inoculating the embryo explant with a Rhizobiales bacterium comprising the heterologous polynucleotide. The methods provided herein also include methods of regenerating a genetically modified plant or plant part from a transformed or edited plant cell or explant.

公开(公告)号：US11473094B2

公开(公告)日：2022-10-18

申请号：US17317255

申请日：2021-05-11

Applicant: Monsanto Technology LLC

Inventor： Whitney R. Adams, Jr. ,  Brian J. Martinell ,  Jyoti R. Rout ,  Edward J. Williams

IPC: C12N15/82

Abstract: The present invention provides methods for improving competency of plant cells for bacterial-mediated transformation comprising contacting the plant cells with an effective amount of polyethylene glycol (PEG) for a period of time prior to transformation. The ability to store and maintain competent plant cells for transformation and tissue culture allows more efficient planning and execution of large-scale experiments by providing flexibility of peak production hours, or during unplanned disruptions in the production process. These methods are useful in preserving the viability of plant cells in various storage conditions, thus improving their competency for transformation and tissue culture.

公开(公告)号：US20140096278A1

公开(公告)日：2014-04-03

申请号：US14077961

申请日：2013-11-12

Applicant: Monsanto Technology, LLC

Inventor： Jennifer R. Hicks ,  Brenda A. Lowe ,  Brian J. Martinell ,  John Phillips ,  John P. Tamulonis ,  Edward J. Williams

IPC: C12N15/82 ,  A01H5/00

CPC classification number: C12N15/8289 ,  A01H1/04 ,  A01H5/00 ,  C12N15/8201 ,  C12N15/8205 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8274 ,  C12N15/8281 ,  C12N15/8282 ,  C12N15/8283 ,  C12N15/8286

Abstract: The invention is directed, in an embodiment, to a method for producing a transgenic plant comprising: providing a substantially homozygous plant line suitable for transformation; selecting a subline of the plant line having reduced heterogeneity; transforming plant materials from the subline with a transgenic construct that confers a desired trait to at least one transformed plant; recovering at least one transgenic event from the transformation step; and selecting a transgenic event exhibiting a desirable level of the desired trait using plants of the subline as control. The invention is also directed to a method for producing a plant having a desired trait. The invention also provides transgenic plants produced according to these methods.

Abstract translation: 本发明在一个实施方案中涉及生产转基因植物的方法，其包括：提供适于转化的基本上纯合的植物系; 选择具有减小的异质性的植物系的一个子系; 用具有将所需性状赋予至少一种转化植物的转基因构建体从该亚系转化植物材料; 从转化步骤回收至少一个转基因事件; 并且使用该子系的植物作为对照选择表现出期望性状的期望水平的转基因事件。 本发明还涉及一种用于生产具有所需性状的植物的方法。 本发明还提供根据这些方法生产的转基因植物。

公开(公告)号：US20240093222A1

公开(公告)日：2024-03-21

申请号：US18480381

申请日：2023-10-03

Applicant: Monsanto Technology LLC

Inventor： Yurong Chen ,  Brian J. Martinell ,  Anatoly Rivlin ,  Ashok Shrawat ,  Yuechun Wan ,  Edward J. Williams ,  Xudong Ye

IPC: C12N15/82

CPC classification number: C12N15/8275 ,  C12N15/8274

Abstract: The present invention provides methods for the production of viable explants from mature corn seeds, wherein the explant comprises the apical portion of the embryo axis of the corn seed. The present invention also relates to methods for producing such explants and for transforming the explants with a heterologous DNA.

公开(公告)号：US20220340925A1

公开(公告)日：2022-10-27

申请号：US13873092

申请日：2013-04-29

Applicant: Monsanto Technology LLC

Inventor： Yurong Chen ,  Brian J. Martinell ,  Anatoly Rivlin ,  Yuechun Wan ,  Edward J. Williams ,  Xudong Ye ,  Ashok Shrawat

IPC: C12N15/82

Abstract: The present invention provides methods for the production of viable explants from mature corn seeds, wherein the explant comprises the apical portion of the embryo axis of the corn seed. The present invention also relates to methods for producing such explants and for transforming the explants with a heterologous DNA.

公开(公告)号：US20210332375A1

公开(公告)日：2021-10-28

申请号：US17317255

申请日：2021-05-11

Applicant: Monsanto Technology LLC

Inventor： Whitney R. Adams, JR. ,  Brian J. Martinell ,  Jyoti R. Rout ,  Edward J. Williams

IPC: C12N15/82

Abstract: The present invention provides methods for improving competency of plant cells for bacterial-mediated transformation comprising contacting the plant cells with an effective amount of polyethylene glycol (PEG) for a period of time prior to transformation. The ability to store and maintain competent plant cells for transformation and tissue culture allows more efficient planning and execution of large-scale experiments by providing flexibility of peak production hours, or during unplanned disruptions in the production process. These methods are useful in preserving the viability of plant cells in various storage conditions, thus improving their competency for transformation and tissue culture.

公开(公告)号：US11091767B2

公开(公告)日：2021-08-17

申请号：US16516025

申请日：2019-07-18

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Whitney R. Adams, Jr. ,  Brian J. Martinell ,  Jyoti R. Rout ,  Edward J. Williams

IPC: C12N15/82

Abstract: The present invention provides methods for improving competency of plant cells, such as corn plant immature embryo for bacterial-mediated transformation comprising contacting the plant cells, such as corn plant immature embryo with an effective amount of polyethylene glycol (PEG) for a period of time prior to transformation. The ability to store and maintain competent plant cells, such as corn plant immature embryo for transformation and tissue culture allows more efficient planning and execution of large-scale experiments by providing flexibility of peak production hours, or during unplanned disruptions in the production process. These methods are useful in preserving the viability of plant cells, such as corn plant immature embryo in various storage conditions, thus improving their competency for transformation and tissue culture.

公开(公告)号：US20200010838A1

公开(公告)日：2020-01-09

申请号：US16516025

申请日：2019-07-18

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Whitney R. Adams, JR. ,  Brian J. Martinell ,  Jyoti R. Rout ,  Edward J. Williams

IPC: C12N15/82

Abstract: The present invention provides methods for improving competency of plant cells for bacterial-mediated transformation comprising contacting the plant cells with an effective amount of polyethylene glycol (PEG) for a period of time prior to transformation. The ability to store and maintain competent plant cells for transformation and tissue culture allows more efficient planning and execution of large-scale experiments by providing flexibility of peak production hours, or during unplanned disruptions in the production process. These methods are useful in preserving the viability of plant cells in various storage conditions, thus improving their competency for transformation and tissue culture.