公开(公告)号：US20150135363A1

公开(公告)日：2015-05-14

申请号：US14534917

申请日：2014-11-06

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Brandi Chiapelli ,  Matt W. Dimmic ,  Deryck Jeremy Williams ,  Jennifer Sheppard ,  John D. Bradley ,  Carl Diehl ,  Michelle L. Gasper ,  Bingli Gao ,  James P. McCarter

IPC: C12N15/82 ,  C12N15/113

CPC classification number: C12N15/8285 ,  C12N15/113 ,  C12N15/8218 ,  C12N2310/14 ,  Y02A40/164

Abstract: The present invention discloses gene targets, constructs and methods for the genetic control of plant disease caused by nematodes of the genus Pratylenchus (root lesion nematodes). The present invention relates to achieving a plant protective effect through the identification of target coding sequences and the use of recombinant DNA technologies for post-transcriptionally repressing or inhibiting expression of the target coding sequences in the cells of plant-parasitic nematodes. The disclosed gene targets are conserved and expected to be essential and sensitive to RNAi perturbation in different Pratylenchus species, facilitating genus-wide targeting by RNA interference.

Abstract translation: 本发明公开了由Pratylenchus属（线虫线虫）线虫引起的植物病害遗传控制的基因靶标，构建体​​和方法。 本发明涉及通过鉴定靶编码序列和使用重组DNA技术来实现植物保护作用，用于转录后抑制或抑制植物寄生线虫细胞中靶编码序列的表达。 所公开的基因靶点是保守的，并且预期对于不同Pratylenchus物种中的RNAi扰动是必需的并且是敏感的，通过RNA干扰促进基因范围的靶向。

公开(公告)号：US20180312867A1

公开(公告)日：2018-11-01

申请号：US15979061

申请日：2018-05-14

Applicant: Monsanto Technology LLC

Inventor： Andrey A. Boukharov ,  Zijin Du ,  Liang Guo ,  Michelle C. Hresko ,  David K. Kovalic ,  Zhaolong Li ,  Maolong Lu ,  James P. McCarter ,  Nancy M. Miller ,  Mark Vaudin ,  Deryck J. Williams ,  Wei Wu

IPC: C12N15/82 ,  C12Q1/6888 ,  C07K14/435 ,  A01N57/16 ,  A61K31/713 ,  C12N15/113 ,  A23L33/105

Abstract: The invention relates to identifying and evaluating target coding sequences for control of plant parasitic nematodes by inhibiting one or more biological functions, and their use. The invention provides methods and compositions for identification of such sequences and for the control of a plant-parasitic nematode population. By feeding one or more recombinant double stranded RNA molecules provided by the invention to the nematode, a reduction in disease may be obtained through suppression of nematode gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and the plant cells and plants obtained thereby.

公开(公告)号：US09790516B2

公开(公告)日：2017-10-17

申请号：US14534917

申请日：2014-11-06

Applicant: Monsanto Technology LLC

Inventor： Brandi Chiapelli ,  Matt W. Dimmic ,  Deryck Jeremy Williams ,  Jennifer Sheppard ,  John D. Bradley ,  Carl Diehl ,  Michelle L. Gasper ,  Bingli Gao ,  James P. McCarter

IPC: C12N15/82 ,  C12N15/113

CPC classification number: C12N15/8285 ,  C12N15/113 ,  C12N15/8218 ,  C12N2310/14 ,  Y02A40/164

Abstract: The present invention discloses gene targets, constructs and methods for the genetic control of plant disease caused by nematodes of the genus Pratylenchus (root lesion nematodes). The present invention relates to achieving a plant protective effect through the identification of target coding sequences and the use of recombinant DNA technologies for post-transcriptionally repressing or inhibiting expression of the target coding sequences in the cells of plant-parasitic nematodes. The disclosed gene targets are conserved and expected to be essential and sensitive to RNAi perturbation in different Pratylenchus species, facilitating genus-wide targeting by RNA interference.

公开(公告)号：US20170247718A1

公开(公告)日：2017-08-31

申请号：US15452504

申请日：2017-03-07

Applicant: Monsanto Technology LLC

Inventor： John D. Bradley ,  Brandi J. Chiapelli ,  Bingli Gao ,  James P. McCarter ,  Michelle L. Gasper ,  Deryck Jeremy Williams

IPC: C12N15/82 ,  A01H4/00 ,  A01N63/02 ,  C12N15/113

CPC classification number: C12N15/8285 ,  A01N65/00 ,  C07K14/4354 ,  C12N15/113 ,  C12N15/8218 ,  C12N15/8261 ,  C12N15/8273 ,  C12N2310/14 ,  Y02A40/164

Abstract: The present invention discloses gene targets, constructs and methods for the genetic control of plant disease caused by nematodes of the genus Meloidogyne (root knot nematodes). The present invention relates to achieving a plant protective effect through the identification of target coding sequences and the use of recombinant DNA technologies for post-transcriptionally repressing or inhibiting expression of the target coding sequences in the cells of plant-parasitic nematodes. The disclosed gene targets show significant conservation at the nucleotide level between orthologs from different Meloidogyne species, facilitating genus-wide targeting by RNA interference.

公开(公告)号：US09388409B2

公开(公告)日：2016-07-12

申请号：US14011647

申请日：2013-08-27

Applicant: Monsanto Technology LLC

Inventor： Andrey A. Boukharov ,  Zijin Du ,  Liang Guo ,  Michelle C. Hresko ,  David K. Kovalic ,  Zhaolong Li ,  Maolong Lu ,  James P. McCarter ,  Nancy M. Miller ,  Mark Vaudin ,  Deryck J. Williams ,  Wei Wu

IPC: C12N15/82 ,  C12N15/87 ,  A01H1/00 ,  C12N15/113 ,  C07K14/435 ,  A61K31/713

CPC classification number: C12N15/8285 ,  A01N57/16 ,  A23L33/105 ,  A23V2002/00 ,  A61K31/713 ,  C07K14/435 ,  C07K14/4354 ,  C12N15/113 ,  C12N15/1137 ,  C12N15/8261 ,  C12N15/8273 ,  C12N2310/11 ,  C12Q1/6888 ,  Y02A40/164

Abstract: The invention relates to identifying and evaluating target coding sequences for control of plant parasitic nematodes by inhibiting one or more biological functions, and their use. The invention provides methods and compositions for identification of such sequences and for the control of a plant-parasitic nematode population. By feeding one or more recombinant double stranded RNA molecules provided by the invention to the nematode, a reduction in disease may be obtained through suppression of nematode gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and the plant cells and plants obtained thereby.

公开(公告)号：US20190241906A1

公开(公告)日：2019-08-08

申请号：US16242869

申请日：2019-01-08

Applicant: Monsanto Technology LLC

Inventor： Andrey A. Boukharov ,  Zijin Du ,  Liang Gou ,  Michelle C. Hresko ,  David K. Kovalic ,  Zhaolong Li ,  Maolong Lu ,  James P. McCarter ,  Nancy M. Miller ,  Mark Vaudin ,  Deryck J. Williams ,  Wei Wu

IPC: C12N15/82 ,  A61K31/713 ,  C12N15/113 ,  A01N57/16 ,  C12Q1/6888 ,  C07K14/435 ,  A23L33/105

CPC classification number: C12N15/8285 ,  A01N57/16 ,  A23L33/105 ,  A23V2002/00 ,  A61K31/713 ,  C07K14/435 ,  C07K14/4354 ,  C12N15/113 ,  C12N15/1137 ,  C12N15/8261 ,  C12N15/8273 ,  C12N2310/11 ,  C12Q1/6888 ,  Y02A40/164

Abstract: The invention relates to identifying and evaluating target coding sequences for control of plant parasitic nematodes by inhibiting one or more biological functions, and their use. The invention provides methods and compositions for identification of such sequences and for the control of a plant-parasitic nematode population. By feeding one or more recombinant double stranded RNA molecules provided by the invention to the nematode, a reduction in disease may be obtained through suppression of nematode gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and the plant cells and plants obtained thereby.

公开(公告)号：US10174340B2

公开(公告)日：2019-01-08

申请号：US15194355

申请日：2016-06-27

Applicant: Monsanto Technology LLC

Inventor： Andrey A. Boukharov ,  Zijin Du ,  Liang Guo ,  Michelle C. Hresko ,  David K. Kovalic ,  Zhaolong Li ,  Maolong Lu ,  James P. McCarter ,  Nancy M. Miller ,  Mark Vaudin ,  Deryck J. Williams ,  Wei Wu

IPC: C12N15/82 ,  C07K14/435 ,  A61K31/713 ,  C12N15/113 ,  A23L33/105 ,  A01N57/16 ,  C12Q1/6888

Abstract: The invention relates to identifying and evaluating target coding sequences for control of plant parasitic nematodes by inhibiting one or more biological functions, and their use. The invention provides methods and compositions for identification of such sequences and for the control of a plant-parasitic nematode population. By feeding one or more recombinant double stranded RNA molecules provided by the invention to the nematode, a reduction in disease may be obtained through suppression of nematode gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and the plant cells and plants obtained thereby.

公开(公告)号：US09988642B2

公开(公告)日：2018-06-05

申请号：US14714068

申请日：2015-05-15

Applicant: Monsanto Technology LLC

Inventor： Andrey A. Boukharov ,  Zijin Du ,  Liang Guo ,  Michelle C. Hresko ,  David K. Kovalic ,  Zhaolong Li ,  Maolong Lu ,  James P. McCarter ,  Nancy M. Miller ,  Mark Vaudin ,  Deryck J. Williams ,  Wei Wu

IPC: C12N15/82 ,  C07K14/435 ,  A61K31/713 ,  C12N15/113 ,  A23L33/105 ,  A01N57/16 ,  C12Q1/68

CPC classification number: C12N15/8285 ,  A01N57/16 ,  A23L33/105 ,  A23V2002/00 ,  A61K31/713 ,  C07K14/435 ,  C07K14/4354 ,  C12N15/113 ,  C12N15/1137 ,  C12N15/8261 ,  C12N15/8273 ,  C12N2310/11 ,  C12Q1/6888 ,  Y02A40/164

Abstract: The invention relates to identifying and evaluating target coding sequences for control of plant parasitic nematodes by inhibiting one or more biological functions, and their use. The invention provides methods and compositions for identification of such sequences and for the control of a plant-parasitic nematode population. By feeding one or more recombinant double stranded RNA molecules provided by the invention to the nematode, a reduction in disease may be obtained through suppression of nematode gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and the plant cells and plants obtained thereby.

公开(公告)号：US10829781B2

公开(公告)日：2020-11-10

申请号：US15979061

申请日：2018-05-14

Applicant: Monsanto Technology LLC

Inventor： Andrey A. Boukharov ,  Zijin Du ,  Liang Guo ,  Michelle C. Hresko ,  David K. Kovalic ,  Zhaolong Li ,  Maolong Lu ,  James P. McCarter ,  Nancy M. Miller ,  Mark Vaudin ,  Deryck J. Williams ,  Wei Wu

IPC: C12N15/82 ,  C07K14/435 ,  A61K31/713 ,  C12N15/113 ,  A23L33/105 ,  A01N57/16 ,  C12Q1/6888

Abstract: The invention relates to identifying and evaluating target coding sequences for control of plant parasitic nematodes by inhibiting one or more biological functions, and their use. The invention provides methods and compositions for identification of such sequences and for the control of a plant-parasitic nematode population. By feeding one or more recombinant double stranded RNA molecules provided by the invention to the nematode, a reduction in disease may be obtained through suppression of nematode gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and the plant cells and plants obtained thereby.

公开(公告)号：US10233462B2

公开(公告)日：2019-03-19

申请号：US15452504

申请日：2017-03-07

Applicant: Monsanto Technology LLC

Inventor： John D. Bradley ,  Brandi J. Chiapelli ,  Bingli Gao ,  James P. McCarter ,  Michelle L. Gasper ,  Deryck Jeremy Williams

IPC: C12N15/113 ,  C12N15/82 ,  C07K14/435 ,  A01N65/00

Abstract: The present invention discloses gene targets, constructs and methods for the genetic control of plant disease caused by nematodes of the genus Meloidogyne (root knot nematodes). The present invention relates to achieving a plant protective effect through the identification of target coding sequences and the use of recombinant DNA technologies for post-transcriptionally repressing or inhibiting expression of the target coding sequences in the cells of plant-parasitic nematodes. The disclosed gene targets show significant conservation at the nucleotide level between orthologs from different Meloidogyne species, facilitating genus-wide targeting by RNA interference.