公开(公告)号：US20170121388A1

公开(公告)日：2017-05-04

申请号：US15319434

申请日：2015-06-09

Applicant: MORPHOSYS AG

Inventor： Sebastian Jaeger ,  Daniela Daubert ,  Kathrin Goetz

IPC: C07K14/715 ,  C12N15/86 ,  C12N15/62 ,  C07K14/005 ,  C12N7/00

CPC classification number: C07K14/7158 ,  A61K39/00 ,  C07K14/005 ,  C07K14/705 ,  C07K2319/00 ,  C07K2319/40 ,  C12N7/00 ,  C12N15/62 ,  C12N15/86 ,  C12N2740/13023 ,  C12N2740/16023 ,  C12N2810/855

Abstract: The present disclosure relates to fusion proteins that are highly useful for the generation of virus-like particles for the display of membrane spanning proteins. Related embodiments, methods and uses are disclosed.

公开(公告)号：US10273285B2

公开(公告)日：2019-04-30

申请号：US15319434

申请日：2015-06-09

Applicant: MORPHOSYS AG

Inventor： Sebastian Jaeger ,  Daniela Daubert ,  Kathrin Goetz

IPC: C07K14/715 ,  C07K14/005 ,  C12N7/00 ,  C12N15/62 ,  C12N15/86 ,  C07K14/705 ,  A61K39/00

Abstract: The present disclosure relates to fusion proteins that are highly useful for the generation of virus-like particles for the display of membrane spanning proteins. Related embodiments, methods and uses are disclosed.

公开(公告)号：US09689014B2

公开(公告)日：2017-06-27

申请号：US14368513

申请日：2013-01-23

Applicant: MorphoSys AG

Inventor： Stefan Haertle ,  Sebastian Jaeger ,  Daniela Daubert

IPC: C12N15/09 ,  C12P21/00 ,  C07K1/22 ,  C12N15/62 ,  C12P21/02

CPC classification number: C12P21/00 ,  C07K1/22 ,  C07K2319/00 ,  C07K2319/35 ,  C12N15/62 ,  C12P21/02

Abstract: The present disclosure provides a method to express and purify polypeptides and proteins. In the present disclosure the use of lysozyme as a fusion partner is disclosed. Furthermore, purification methods to isolate lysozyme-tagged polypeptides and proteins via lysozyme-specific antibodies are described. More specifically, the present disclosure provides a method to express and purify monomeric polypeptides and proteins by using lysozyme as a tag.

公开(公告)号：US20140349343A1

公开(公告)日：2014-11-27

申请号：US14368513

申请日：2013-01-23

Applicant: MorphoSys AG

Inventor： Stefan Haertle ,  Sebastian Jaeger ,  Daniela Daubert

IPC: C12P21/00

CPC classification number: C12P21/00 ,  C07K1/22 ,  C07K2319/00 ,  C07K2319/35 ,  C12N15/62 ,  C12P21/02

Abstract: The present disclosure provides a method to express and purify polypeptides and proteins. In the present disclosure the use of lysozyme as a fusion partner is disclosed. Furthermore, purification methods to isolate lysozyme-tagged polypeptides and proteins via lysozyme-specific antibodies are described. More specifically, the present disclosure provides a method to express and purify monomeric polypeptides and proteins by using lysozyme as a tag.

Abstract translation: 本公开提供了表达和纯化多肽和蛋白质的方法。 在本公开中公开了溶菌酶作为融合配偶体的用途。 此外，描述了通过溶菌酶特异性抗体分离溶菌酶标记的多肽和蛋白质的纯化方法。 更具体地，本公开提供了通过使用溶菌酶作为标签来表达和纯化单体多肽和蛋白质的方法。