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公开(公告)号：US08575322B2
公开(公告)日：2013-11-05
申请号：US13256028
申请日：2010-03-12
申请人： Mulakkapurath Narayanan Manoj , Venkata Ramachandra Rao Vasamsetty , Madhuri Baliga , Kirubakaran Naveen Kumar , Chandrasekhar Bhaskaran Nair , Pillarisetti Venkata Subbarao
发明人： Mulakkapurath Narayanan Manoj , Venkata Ramachandra Rao Vasamsetty , Madhuri Baliga , Kirubakaran Naveen Kumar , Chandrasekhar Bhaskaran Nair , Pillarisetti Venkata Subbarao
CPC分类号： C07K1/18 , C07K14/62 , C12N2800/22
摘要： The present disclosure relates to a field of recombinant DNA therapeutics. It involves the bio-informatics design, synthesis of artificial gene for human insulin precursor including leader peptide coding sequence, cloning in an expression vector and expression in an organism, preferably Pichia pastoris. The present disclosure also relates to methods of downstream processing for obtaining protein precursor molecules and subsequent conversion of precursor molecules to functional proteins.
摘要翻译： 本公开涉及重组DNA治疗领域。 它包括生物信息学设计，人胰岛素前体的人造基因的合成，包括前导肽编码序列，表达载体中的克隆和生物体中优选巴斯德毕赤酵母表达。 本公开还涉及用于获得蛋白质前体分子和随后将前体分子转化成功能性蛋白质的下游处理方法。
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公开(公告)号：US20120077966A1
公开(公告)日：2012-03-29
申请号：US13256028
申请日：2010-03-12
申请人： Mulakkapurath Narayanan Manoj , Venkata Ramachandra Rao Vasamsetty , Madhuri Baliga , Kirubakaran Naveen Kumar , Chandrasekhar Bhaskaran Nair , Pillarisetti Venkata Subbarao
发明人： Mulakkapurath Narayanan Manoj , Venkata Ramachandra Rao Vasamsetty , Madhuri Baliga , Kirubakaran Naveen Kumar , Chandrasekhar Bhaskaran Nair , Pillarisetti Venkata Subbarao
CPC分类号： C07K1/18 , C07K14/62 , C12N2800/22
摘要： The present disclosure relates to a field of recombinant DNA therapeutics. It involves the bio-informatics design, synthesis of artificial gene for human insulin precursor including leader peptide coding sequence, cloning in an expression vector and expression in an organism, preferably Pichia pastoris. The present disclosure also relates to methods of downstream processing for obtaining protein precursor molecules and subsequent conversion of precursor molecules to functional proteins.
摘要翻译： 本公开涉及重组DNA治疗领域。 它包括生物信息学设计，人胰岛素前体的人造基因的合成，包括前导肽编码序列，表达载体中的克隆和生物体中优选巴斯德毕赤酵母表达。 本公开还涉及用于获得蛋白质前体分子和随后将前体分子转化成功能性蛋白质的下游处理方法。
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公开(公告)号：US20130203150A1
公开(公告)日：2013-08-08
申请号：US13520685
申请日：2011-01-06
申请人： Phani Kumar Pullela , Mulakkapurath Narayanan Manoj , Santhosh Kumar Gandhavalla , Mitchell Preetham Pinto , Chandrasekhar Bhaskaran Nair , Pillarisetti Venkata Subbarao
发明人： Phani Kumar Pullela , Mulakkapurath Narayanan Manoj , Santhosh Kumar Gandhavalla , Mitchell Preetham Pinto , Chandrasekhar Bhaskaran Nair , Pillarisetti Venkata Subbarao
IPC分类号： C07H1/08
CPC分类号： C07H1/08 , C12N15/10 , C12N15/1006
摘要： The present disclosure provides a method to isolate natural & artificial nucleic acids like deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and peptide nucleic acid (PNA) from a solid or liquid sample using cotton. The cotton packed is such that, a solution containing nucleic acids passes through it and the nucleic acids in solution are bound to the cotton in a medium optimal for binding. The nucleic acids are bound to cotton in such a way that, the bound nucleic acids can withstand multiple washes with liquid comprising water and gets eluted in an aqueous buffer, with which eluted nucleic acids can be directly used for amplification using PCR or for any other biochemical or molecular biology needs.
摘要翻译： 本公开提供了使用棉花从固体或液体样品分离天然和人造核酸如脱氧核糖核酸（DNA），核糖核酸（RNA）和肽核酸（PNA）的方法。 包装的棉花使得含有核酸的溶液通过它，并且溶液中的核酸在最适于结合的培养基中与棉花结合。 核酸以这样的方式结合棉花，使得结合的核酸可以经受含有水的液体的多次洗涤，并在水性缓冲液中洗脱，其中洗脱的核酸可以直接用于使用PCR或任何其它的扩增 生物化学或分子生物学的需要。
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公开(公告)号：US08986976B2
公开(公告)日：2015-03-24
申请号：US13520685
申请日：2011-01-06
申请人： Phani Kumar Pullela , Mulakkapurath Narayanan Manoj , Santhosh Kumar Gandhavalla , Mitchell Preetham Pinto , Chandrasekhar Bhaskaran Nair , Pillarisetti Venkata Subbarao
发明人： Phani Kumar Pullela , Mulakkapurath Narayanan Manoj , Santhosh Kumar Gandhavalla , Mitchell Preetham Pinto , Chandrasekhar Bhaskaran Nair , Pillarisetti Venkata Subbarao
CPC分类号： C07H1/08 , C12N15/10 , C12N15/1006
摘要： The present disclosure provides a method to isolate natural & artificial nucleic acids like deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and peptide nucleic acid (PNA) from a solid or liquid sample using cotton. The cotton packed is such that, a solution containing nucleic acids passes through it and the nucleic acids in solution are bound to the cotton in a medium optimal for binding. The nucleic acids are bound to cotton in such a way that, the bound nucleic acids can withstand multiple washes with liquid comprising water and gets eluted in an aqueous buffer, with which eluted nucleic acids can be directly used for amplification using PCR or for any other biochemical or molecular biology needs.
摘要翻译： 本公开提供了使用棉花从固体或液体样品分离天然和人造核酸如脱氧核糖核酸（DNA），核糖核酸（RNA）和肽核酸（PNA）的方法。 包装的棉花使得含有核酸的溶液通过它，并且溶液中的核酸在最适于结合的培养基中与棉花结合。 核酸以这样的方式结合棉花，使得结合的核酸可以经受含有水的液体的多次洗涤，并在水性缓冲液中洗脱，其中洗脱的核酸可以直接用于使用PCR或任何其它的扩增 生物化学或分子生物学的需要。
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公开(公告)号：US20110306046A1
公开(公告)日：2011-12-15
申请号：US13202842
申请日：2010-01-27
申请人： Manjula Jagannath , Chandrasekhar Bhaskaran Nair , Pillarisetti Venkata Subbarao , Mulakkapurath Narayanan Manoj , Shamamandri Markandaiah Shashirekha
发明人： Manjula Jagannath , Chandrasekhar Bhaskaran Nair , Pillarisetti Venkata Subbarao , Mulakkapurath Narayanan Manoj , Shamamandri Markandaiah Shashirekha
CPC分类号： C12Q1/6893 , Y02A50/58
摘要： The present disclosure gives description of a method used for the detection and quantification of malarial infection caused either by Plasmodium falciparum or Plasmodium vivax using nucleic acids isolated from blood samples by employing Oligonucleotide probes. The method employed here for detection is by Real time PCR.
摘要翻译： 本公开描述了使用通过使用寡核苷酸探针从血液样品分离的核酸来检测和定量由恶性疟原虫或间日疟原虫引起的疟疾感染的方法的描述。 这里用于检测的方法是通过实时PCR。
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