公开(公告)号：US20220099616A1

公开(公告)日：2022-03-31

申请号：US17483840

申请日：2021-09-24

申请人： NATIONAL SUN YAT-SEN UNIVERSITY

发明人： Hong-Wei Yang ,  Nan-Si Li ,  Ying-Pei Hsu ,  Hao-Han Pang

IPC分类号： G01N27/414 ,  G01N27/36 ,  G01N27/30 ,  C03C17/32 ,  B82Y40/00

摘要： A method for manufacturing a glass-based biosensor is used to solve the problem of the use of a solution containing a strong acid or a strong base or of an oxygen plasma treatment. The method comprises modifying a silicon-containing substrate by an alcohol solution to form negative charges on at least one coupling surface of the silicon-containing substrate. A least one active layer of polymer having positive charges is formed on the at least one surface of the silicon-containing substrate, respectively. Each of the at least one active layer of polymer has a coupling surface and an active surface opposite to the coupling surface, and the at least one active layer of polymer couples to the silicon-containing substrate via the coupling surface. A plurality of capture biomolecules couples to the active surface. The invention also discloses the biosensor manufacture by the method.

公开(公告)号：US20240336651A1

公开(公告)日：2024-10-10

申请号：US18748165

申请日：2024-06-20

申请人： NATIONAL SUN YAT-SEN UNIVERSITY

发明人： Hong-Wei Yang ,  Nan-Si Li ,  Ying-Pei Hsu ,  Hao-Han Pang

IPC分类号： C07K2/00 ,  A61K38/16 ,  B82Y40/00 ,  C03C17/32 ,  C07K14/005 ,  G01N21/78 ,  G01N27/414 ,  G01N33/543

CPC分类号： C07K2/00 ,  A61K38/162 ,  C03C17/32 ,  C07K14/005 ,  G01N21/78 ,  G01N27/4145 ,  G01N33/54393 ,  B82Y40/00 ,  C03C2217/70 ,  C07K2319/30 ,  C07K2319/735 ,  C07K2319/74 ,  C12N2795/00023

摘要： A method for manufacturing a glass-based biosensor is used to solve the problem of the use of a solution containing a strong acid or a strong base or of an oxygen plasma treatment. The method comprises modifying a silicon-containing substrate by an alcohol solution to form negative charges on at least one coupling surface of the silicon-containing substrate. A least one active layer of polymer having positive charges is formed on the at least one surface of the silicon-containing substrate, respectively. Each of the at least one active layer of polymer has a coupling surface and an active surface opposite to the coupling surface, and the at least one active layer of polymer couples to the silicon-containing substrate via the coupling surface. A plurality of capture biomolecules couples to the active surface.

公开(公告)号：US20220098236A1

公开(公告)日：2022-03-31

申请号：US17485680

申请日：2021-09-27

申请人： NATIONAL SUN YAT-SEN UNIVERSITY

发明人： Hong-Wei Yang ,  Hao-Han Pang ,  Ying-Pei Hsu ,  Nan-Si Li

IPC分类号： C07K2/00

摘要： A fusion peptide for forming a virus-like particle is used to solve the problem that a target biomolecule is chemically conjugated to the virus-like particle. The fusion peptide includes a vehicle fragment and a capture fragment connected to the vehicle fragment. The vehicle fragment is for forming the virus-like particle, and the capture fragment is for forming a capture peptide which is not enveloped by the virus-like particle and is adapted to specific bind the target biomolecule.