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公开(公告)号：US11891629B2
公开(公告)日：2024-02-06
申请号：US16637656
申请日：2018-08-08
发明人： Jasper Clube
CPC分类号： C12N7/00 , A61K35/74 , C12N2310/20 , C12N2795/00023 , C12N2795/00032 , C12N2795/00052 , C12N2800/80
摘要： The invention provides propagator cells and methods for propagating phage and transduction particles.
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公开(公告)号：US09518095B2
公开(公告)日：2016-12-13
申请号：US14247097
申请日：2014-04-07
申请人： KUROS BIOSCIENCES AG
CPC分类号： C07K14/005 , A61K2039/5256 , A61K2039/5258 , C12N7/00 , C12N2795/00023 , C12N2795/00051 , C12N2795/10051 , C12N2795/10061 , C12N2795/18022 , C12N2795/18052
摘要： This invention provides a robust fermentation process for the expression of a capsid protein of a bacteriophage which is forming a VLP by self-assembly, wherein the process is scalable to a commercial production scale and wherein the expression rate of the capsid protein is controlled to obtain improved yield of soluble capsid protein. This is achieved by combining the advantages of fed-batch culture and of lactose induced expression systems with specific process parameters providing improved repression of the promoter during the growth phase and high plasmid retention throughout the process.
摘要翻译： 本发明提供了用于通过自组装形成VLP的噬菌体的衣壳蛋白的表达的鲁棒发酵方法，其中该方法可扩展到商业生产规模，并且其中控制衣壳蛋白的表达速率以获得 提高可溶性衣壳蛋白的产量。 这是通过将补料分批培养和乳糖诱导表达系统的优点与具体工艺参数相结合来实现的，其提供了在生长阶段改进的启动子抑制以及整个过程中的高质粒保留。
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公开(公告)号：US20240336651A1
公开(公告)日：2024-10-10
申请号：US18748165
申请日：2024-06-20
发明人： Hong-Wei Yang , Nan-Si Li , Ying-Pei Hsu , Hao-Han Pang
IPC分类号： C07K2/00 , A61K38/16 , B82Y40/00 , C03C17/32 , C07K14/005 , G01N21/78 , G01N27/414 , G01N33/543
CPC分类号： C07K2/00 , A61K38/162 , C03C17/32 , C07K14/005 , G01N21/78 , G01N27/4145 , G01N33/54393 , B82Y40/00 , C03C2217/70 , C07K2319/30 , C07K2319/735 , C07K2319/74 , C12N2795/00023
摘要： A method for manufacturing a glass-based biosensor is used to solve the problem of the use of a solution containing a strong acid or a strong base or of an oxygen plasma treatment. The method comprises modifying a silicon-containing substrate by an alcohol solution to form negative charges on at least one coupling surface of the silicon-containing substrate. A least one active layer of polymer having positive charges is formed on the at least one surface of the silicon-containing substrate, respectively. Each of the at least one active layer of polymer has a coupling surface and an active surface opposite to the coupling surface, and the at least one active layer of polymer couples to the silicon-containing substrate via the coupling surface. A plurality of capture biomolecules couples to the active surface.
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公开(公告)号：US09822361B2
公开(公告)日：2017-11-21
申请号：US14897577
申请日：2014-06-05
申请人： APSE LLC
发明人： Juan Pedro Humberto Arhancet , Juan P. Arhancet , Kimberly Delaney , Kathleen B. Hall , Neena Summers , Edward Oates
IPC分类号： C12N15/11 , C12N15/113 , A61K48/00 , A61K9/51
CPC分类号： C12N15/113 , A61K9/5184 , A61K48/00 , C07K14/005 , C12N15/111 , C12N2310/12 , C12N2310/121 , C12N2310/14 , C12N2310/141 , C12N2310/3519 , C12N2310/531 , C12N2320/32 , C12N2330/50 , C12N2795/00023 , C12N2795/00042 , C12N2795/18122 , C12N2795/18123 , C12N2795/18142
摘要： Novel processes and compositions are described which use viral capsid proteins resistant to hydrolases to prepare virus-like particles to enclose and subsequently isolate and purify target cargo molecules of interest including nucleic acids such as siRNAs and shRNAs, miRNAs, messenger RNAs, small peptides and bioactive molecules.
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5.发明授权Immunogenic respiratory syncytial virus glycoprotein-containing VLPs and related compositions, constructs, and therapeutic methods 有权含免疫原性呼吸道合胞病毒糖蛋白的VLP及相关组合物，构建体和治疗方法公开(公告)号：US09511135B2
公开(公告)日：2016-12-06
申请号：US13820891
申请日：2011-09-13
IPC分类号： A61K39/155 , A61K39/295 , C07K14/005 , A61K39/12 , A61K47/48 , A61K39/00
CPC分类号： A61K39/155 , A61K39/12 , A61K39/295 , A61K47/69 , A61K47/6901 , A61K47/6929 , A61K2039/5256 , A61K2039/5258 , A61K2039/55566 , A61K2039/6075 , C07K14/005 , C07K2319/40 , C12N7/00 , C12N2760/18523 , C12N2760/18534 , C12N2795/00023
摘要： The invention provides immunotherapeutic and prophylactic bacteriophage viral-like particle (VLPs) which are useful in the treatment and prevention of Respiratory Syncytial Virus (RSV) infections and related disorders, including bronchiolitis and viral pneumonia. Related compositions (e.g. vaccines), nucleic acid constructs, and therapeutic methods are also provided. VLPs and related compositions of the invention induce high titer antibody responses against RSV. VLPs, VLP-containing compositions, and therapeutic methods of the invention induce an immunogenic response against RSV infection, confer immunity against RSV infection, protect against RSV infection, and reduce the likelihood of infection by RSV infection.
摘要翻译： 本发明提供了可用于治疗和预防呼吸道合胞病毒（RSV）感染和相关疾病（包括细支气管炎和病毒性肺炎）的免疫治疗和预防性噬菌体病毒样颗粒（VLP）。 还提供了相关组合物（例如疫苗），核酸构建体和治疗方法。 本发明的VLP和相关组合物诱导针对RSV的高效价抗体应答。 VLP，含VLP的组合物和本发明的治疗方法诱导针对RSV感染的免疫原性应答，赋予针对RSV感染的免疫，防止RSV感染，并降低RSV感染感染的可能性。
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公开(公告)号：US20150104827A1
公开(公告)日：2015-04-16
申请号：US14247097
申请日：2014-04-07
IPC分类号： C07K14/005
CPC分类号： C07K14/005 , A61K2039/5256 , A61K2039/5258 , C12N7/00 , C12N2795/00023 , C12N2795/00051 , C12N2795/10051 , C12N2795/10061 , C12N2795/18022 , C12N2795/18052
摘要： This invention provides a robust fermentation process for the expression of a capsid protein of a bacteriophage which is forming a VLP by self-assembly, wherein the process is scalable to a commercial production scale and wherein the expression rate of the capsid protein is controlled to obtain improved yield of soluble capsid protein. This is achieved by combining the advantages of fed-batch culture and of lactose induced expression systems with specific process parameters providing improved repression of the promoter during the growth phase and high plasmid retention throughout the process.
摘要翻译： 本发明提供了用于通过自组装形成VLP的噬菌体的衣壳蛋白的表达的鲁棒发酵方法，其中该方法可扩展到商业生产规模，并且其中控制衣壳蛋白的表达速率以获得 提高可溶性衣壳蛋白的产量。 这是通过将补料分批培养和乳糖诱导表达系统的优点与具体工艺参数相结合来实现的，其提供了在生长阶段改进的启动子抑制以及整个过程中的高质粒保留。
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7.发明公开公开(公告)号：US20240117323A1
公开(公告)日：2024-04-11
申请号：US18539066
申请日：2023-12-13
发明人： Jasper CLUBE
CPC分类号： C12N7/00 , A61K35/74 , C12N2310/20 , C12N2795/00023 , C12N2795/00032 , C12N2795/00052 , C12N2800/80
摘要： The invention provides propagator cells and methods for propagating phage and transduction particles.
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公开(公告)号：US11873347B2
公开(公告)日：2024-01-16
申请号：US16760394
申请日：2018-10-31
发明人： Jan Steyaert , Els Pardon , Tomasz Uchanski , Wim Vranken
IPC分类号： C07K16/46 , C07K14/195 , G01N23/20 , G01N33/68 , C07K14/705 , C07K14/005 , C12N7/00
CPC分类号： C07K16/465 , C07K14/005 , C07K14/195 , C07K14/705 , C12N7/00 , G01N23/20 , G01N33/6845 , C07K2299/00 , C07K2317/569 , C07K2319/03 , C12N2795/00022 , C12N2795/00023
摘要： The present invention relates to the field of structural biology. More specifically, the present invention relates to novel antigen-binding chimeric proteins, their uses and methods in three-dimensional structural analysis of macromolecules, such as X-ray crystallography and high-resolution Cryo-EM, and their use as a therapeutic, diagnostic, or imaging tool. Even more specifically, the invention relates to a fusion of a scaffold protein and an antigen-binding domain wherein the scaffold protein of said fusion interrupts the Immunoglobulin domain topology to form a rigid chimer.
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公开(公告)号：US20180360935A1
公开(公告)日：2018-12-20
申请号：US16063040
申请日：2016-12-18
申请人： AGILVAX, INC.
CPC分类号： A61K39/0011 , A61K35/76 , A61K38/00 , A61K39/12 , A61K2039/5256 , A61K2039/5258 , A61K2039/572 , A61K2039/585 , A61K2039/812 , A61P35/00 , C12N15/62 , C12N15/86 , C12N2795/00023 , C12N2795/00034 , C12N2795/00041
摘要： Certain embodiments are directed to composition for inducing an immune response against xCT that is directed to cancer stem cells expressing xCT.
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公开(公告)号：US20160177299A1
公开(公告)日：2016-06-23
申请号：US14897577
申请日：2014-06-05
申请人： APSE LLC
发明人： Juan Pedro Humberto ARHANCET , JUAN P. ARHANCET , Kimberly DELANEY , Kathleen B. HALL , Neena SUMMERS , Edward OATES
IPC分类号： C12N15/113
CPC分类号： C12N15/113 , A61K9/5184 , A61K48/00 , C07K14/005 , C12N15/111 , C12N2310/12 , C12N2310/121 , C12N2310/14 , C12N2310/141 , C12N2310/3519 , C12N2310/531 , C12N2320/32 , C12N2330/50 , C12N2795/00023 , C12N2795/00042 , C12N2795/18122 , C12N2795/18123 , C12N2795/18142
摘要： Novel processes and compositions are described which use viral capsid proteins resistant to hydrolases to prepare virus-like particles to enclose and subsequently isolate and purify target cargo molecules of interest including nucleic acids such as siRNAs and shRNAs, miRNAs, messenger RNAs, small peptides and bioactive molecules.
摘要翻译： 描述了新的方法和组合物，其使用对水解酶具有抗性的病毒衣壳蛋白来制备病毒样颗粒以包围并随后分离和纯化目标物质分子，包括核酸如siRNA和shRNA，miRNA，信使RNA，小肽和生物活性 分子。
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