公开(公告)号：US20240101988A1

公开(公告)日：2024-03-28

申请号：US18355170

申请日：2023-07-19

Applicant: Novozymes A/S

Inventor： Carsten Andersen ,  Poul Erik Pedersen ,  Michael Dolberg Rasmussen ,  Carsten Hjort

IPC: C12N9/22 ,  C12N15/63

CPC classification number: C12N9/22 ,  C12N15/63 ,  C07K2319/35 ,  C12Y302/01001

Abstract: The present invention relates to temperature-sensitive variants of a Class-II Cas9 protein, polynucleotides encoding said variants, nucleic acid constructs and expression vectors comprising polynucleotides encoding said variants, host cells expressing said variant, methods of transient repression and expression of one or more genome target sequence using said variants, and use of said variants, polynucleotides, nucleic acid constructs, expression vectors, host cells, and methods.

公开(公告)号：US20200318086A1

公开(公告)日：2020-10-08

申请号：US16652920

申请日：2018-11-07

Applicant: Novozymes A/S

Inventor： Carsten Anderson ,  Poul Erik Pedersen ,  Michael Dolberg Rasmussen ,  Carsten Hjort

IPC: C12N9/22 ,  C12N15/63

Abstract: The present invention relates to temperature-sensitive variants of a Class-II Cas9 protein, polynucleotides encoding said variants, nucleic acid constructs and expression vectors comprising polynucleotides encoding said variants, host cells expressing said variant, methods of transient repression and expression of one or more genome target sequence using said variants, and use of said variants, polynucleotides, nucleic acid constructs, expression vectors, host cells, and methods.

公开(公告)号：US20190185847A1

公开(公告)日：2019-06-20

申请号：US16309651

申请日：2017-07-05

Applicant: Novozymes A/S

Inventor： Michael Dolberg Rasmussen ,  Poul Erik Pedersen ,  Carsten Hjort ,  Barbara Cherry

IPC: C12N15/11 ,  C12N9/22 ,  C12N15/75

CPC classification number: C12N15/11 ,  C12N9/22 ,  C12N15/75 ,  C12N2310/20 ,  C12N2800/80

Abstract: The present invention relates to methods for improving at least one property of a microorganism host cell by repressing the expression of one or more genome target sequence of interest by CRISPR-inhibition as well as the resulting host cells and methods of production emplying said host cells.

公开(公告)号：US20150079628A1

公开(公告)日：2015-03-19

申请号：US14550579

申请日：2014-11-21

Applicant: NOVOZYMES A/S

Inventor： Anders Vikso-Nielsen ,  Carsten Andersen ,  Sven Pedersen ,  Carsten Hjort

IPC: C12P19/14 ,  C12P19/22 ,  C13K11/00 ,  C12P19/02

CPC classification number: C12P19/14 ,  C07K2319/00 ,  C12N9/2408 ,  C12N9/2411 ,  C12N9/2414 ,  C12N9/242 ,  C12N9/2425 ,  C12N9/2428 ,  C12P7/06 ,  C12P19/02 ,  C12P19/16 ,  C12P19/22 ,  C13K11/00 ,  Y02E50/17

Abstract: The present invention relates to a process for enzymatic hydrolysis of granular starch into a soluble starch hydrolysate at a temperature below the initial gelatinization temperature of said granular starch.

Abstract translation: 本发明涉及一种在低于所述颗粒状淀粉的初始糊化温度的温度下将颗粒淀粉酶水解成可溶性淀粉水解产物的方法。