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    Purified gp120 compositions retaining natural conformation
    1.
    发明授权
    Purified gp120 compositions retaining natural conformation 失效
    纯化的gp120组合物保留自然构象

    公开(公告)号：US5614612A

    公开(公告)日：1997-03-25

    申请号：US240073

    申请日：1994-05-09

    申请人： Nancy L. Haigwood ,  Carl Scandella

    发明人： Nancy L. Haigwood ,  Carl Scandella

    IPC分类号： A61K39/00 ,  C07K14/16 ,  C07K1/00 ,  A61K39/21 ,  C07K14/00

    CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2740/16122

    摘要： A method for purifying recombinant HIV gp120 so as to provide a glycopeptide having protein/protein binding properties substantially identical to natural viral HIV gp120, which comprises fractionating a composition containing crude gp120 sequentially using (1) ion exchange chromatography, (2) hydrophobic-interaction chromatography, and (3) size exclusion filtration, collecting at each step a fraction that exhibits specific binding affinity for CD4 peptide. The process is carried out in the absence of any affinity purification steps or any steps (such as reverse-phase HPLC) that use contact protein with organic solvents. The product obtained by this method is a purified, full-length, non-fusion recombinant HIV gp120 glycoprotein having protein/protein-interaction properties substantially identical to gp120 as presented on an HIV virus, including binding affinity for CD4 and binding affinity for at least one antibody capable of neutralizing HIV infectivity.

    摘要翻译： 一种用于纯化重组HIV gp120的方法，以提供具有与天然病毒HIV gp120基本相同的蛋白质/蛋白质结合性质的糖肽，其包括使用（1）离子交换色谱法，（2）疏水相互作用 色谱法和（3）尺寸排阻过滤，在每个步骤收集显示对CD4肽具有特异性结合亲和力的级分。 该方法在没有使用接触蛋白与有机溶剂的任何亲和纯化步骤或任何步骤（例如反相HPLC）的情况下进行。 通过该方法获得的产物是纯化的全长非融合重组HIV gp120糖蛋白，其具有与HIV病毒上呈现的与gp120基本相同的蛋白质/蛋白质相互作用特性，包括对CD4的结合亲和力和对至少的结合亲和力 一种能够中和HIV感染性的抗体。

    Purified GP120 composition retaining natural conformation
    2.
    发明授权
    Purified GP120 composition retaining natural conformation 失效
    纯化的GP120组合物保留自然构象

    公开(公告)号：US5696238A

    公开(公告)日：1997-12-09

    申请号：US439286

    申请日：1995-05-11

    申请人： Nancy L. Haigwood ,  Carl Scandella

    发明人： Nancy L. Haigwood ,  Carl Scandella

    IPC分类号： A61K39/00 ,  C07K14/16 ,  A23J1/00 ,  A61K39/21 ,  C12N7/02

    CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2740/16122

    摘要： A method for purifying recombinant HIV gp120 so as to provide a glycopeptide having protein/protein binding properties substantially identical to natural viral HIV gp120, which comprises fractionating a composition containing crude gp120 sequentially using (1) ion exchange chromatography, (2) hydrophobic-interaction chromatography, and (3) size exclusion filtration, collecting at each step a fraction that exhibits specific binding affinity for CD4 peptide. The process is carried out in the absence of any affinity purification steps or any steps (such as reverse-phase HPLC) that use contact protein with organic solvents. The product obtained by this method is a purified, full-length, non-fusion recombinant HIV gp120 glycoprotein having protein/protein interaction properties substantially identical to gp120 as presented on an HIV virus, including binding affinity for CD4 and binding affinity for at least one antibody capable of neutralizing HIV infectivity.

    摘要翻译： 一种用于纯化重组HIV gp120的方法，以提供具有与天然病毒HIV gp120基本相同的蛋白质/蛋白质结合性质的糖肽，其包括使用（1）离子交换色谱法，（2）疏水相互作用 色谱法和（3）尺寸排阻过滤，在每个步骤收集显示对CD4肽具有特异性结合亲和力的级分。 该方法在没有使用接触蛋白与有机溶剂的任何亲和纯化步骤或任何步骤（例如反相HPLC）的情况下进行。 通过该方法获得的产物是纯化的全长非融合重组HIV gp120糖蛋白，其具有与HIV病毒上呈现的与gp120基本相同的蛋白质/蛋白质相互作用特性，包括对CD4的结合亲和力和对至少一种 能够中和HIV感染性的抗体。

    Purified gp120 composition retaining natural conformation
    3.
    发明授权
    Purified gp120 composition retaining natural conformation 失效
    纯化的gp120组合保留自然构象

    公开(公告)号：US5653985A

    公开(公告)日：1997-08-05

    申请号：US439119

    申请日：1995-05-11

    申请人： Nancy L. Haigwood ,  Carl Scandella

    发明人： Nancy L. Haigwood ,  Carl Scandella

    IPC分类号： A61K39/00 ,  C07K14/16 ,  A61K39/21 ,  A61K39/12 ,  C07K1/00 ,  C07K14/00

    CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N2740/16122

    摘要： A method for purifying recombinant HIV gp120 so as to provide a glycopeptide having protein/protein binding properties substantially identical to natural viral HIV gp120, which comprises fractionating a composition containing crude gp120 sequentially using (1) ion exchange chromatography, (2) hydrophobic-interaction chromatography, and (3) size exclusion filtration, collecting at each step a fraction that exhibits specific binding affinity for CD4 peptide. The process is carried out in the absence of any affinity purification steps or any steps (such as reverse-phase HPLC) that use contact protein with organic solvents. The product obtained by this method is a purified, full-length, non-fusion recombinant HIV gp120 glycoprotein having protein/protein-interaction properties substantially identical to gp120 as presented on an HIV virus, including binding affinity for CD4 and binding affinity for at least one antibody capable of neutralizing HIV infectivity.

    摘要翻译： 一种用于纯化重组HIV gp120的方法，以提供具有与天然病毒HIV gp120基本相同的蛋白质/蛋白质结合性质的糖肽，其包括使用（1）离子交换色谱法，（2）疏水相互作用 色谱法和（3）尺寸排阻过滤，在每个步骤收集对CD4肽具有特异性结合亲和力的级分。 该方法在没有使用接触蛋白与有机溶剂的任何亲和纯化步骤或任何步骤（例如反相HPLC）的情况下进行。 通过该方法获得的产物是纯化的全长非融合重组HIV gp120糖蛋白，其具有与HIV病毒上呈现的与gp120基本相同的蛋白质/蛋白质相互作用特性，包括对CD4的结合亲和力和对至少的结合亲和力 一种能够中和HIV感染性的抗体。