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    IGF-I purification process
    4.
    发明授权
    IGF-I purification process 失效
    IGF-I纯化方法

    公开(公告)号：US5650496A

    公开(公告)日：1997-07-22

    申请号：US422436

    申请日：1995-04-14

    申请人： Russell A. Brierley ,  Joan N. Abrams ,  John M. Hanson ,  Francis C. Maslanka

    发明人： Russell A. Brierley ,  Joan N. Abrams ,  John M. Hanson ,  Francis C. Maslanka

    IPC分类号： C12N15/09 ,  C07K1/18 ,  C07K1/20 ,  C07K1/36 ,  C07K14/65 ,  C12N15/18 ,  C12P21/00 ,  C12R1/84 ,  G01N30/02 ,  C07K14/475

    CPC分类号： C07K14/65 ,  G01N30/02

    摘要： Disclosed is an improved process for obtaining purified, monomeric, intact, correctly-folded insulin-like growth factor-I (also known as somatomedin-C). The improvements, consisting primarily of the addition of an IGF-I unfolding/refolding step and the substitution of a reverse phase chromatography step for a gel filtration chromatography step result in a three-fold increase in final yield. The process includes the following steps, in order: first cation exchange, unfolding/refolding, hydrophobic interaction chromatography, second cation exchange, and reverse phase chromatography.

    摘要翻译： 公开了用于获得纯化的，单体的，完整的，正确折叠的胰岛素样生长因子-I（也称为生长因子C）的改进方法。 主要由IGF-I展开/重折叠步骤的添加和用于凝胶过滤层析步骤的反相色谱步骤的替代的改进导致最终产率增加三倍。 该方法包括以下步骤：首先进行阳离子交换，展开/重折叠，疏水相互作用层析，第二次阳离子交换和反相色谱法。

    IGF-I purification process
    5.
    发明授权
    IGF-I purification process 失效
    IGF-I纯化方法

    公开(公告)号：US06207806B1

    公开(公告)日：2001-03-27

    申请号：US08851162

    申请日：1997-05-05

    申请人： Russell A. Brierley ,  Joan N. Abrams ,  John M. Hanson ,  Francis C. Maslanka

    发明人： Russell A. Brierley ,  Joan N. Abrams ,  John M. Hanson ,  Francis C. Maslanka

    IPC分类号： C07K14475

    CPC分类号： C07K14/65 ,  G01N30/02 ,  B01D15/422

    摘要： Disclosed is an improved process for obtaining purified, monomeric, intact, correctly-folded insulin-like growth factor-I (also known as somatomedin-C). The improvements, consisting primarily of the addition of an IGF-I unfolding/refolding step and the substitution of a reverse phase chromatography step for a gel filtration chromatography step result in a three-fold increase in final yield. The process includes the following steps, in order: first cation exchange, unfolding/refolding, hydrophobic interaction chromatography, second cation exchange, and reverse phase chromatography.

    摘要翻译： 公开了用于获得纯化的，单体的，完整的，正确折叠的胰岛素样生长因子-I（也称为生长因子C）的改进方法。 主要由IGF-I展开/重折叠步骤的添加和用于凝胶过滤层析步骤的反相色谱步骤的替代的改进导致最终产率增加三倍。 该方法包括以下步骤：首先进行阳离子交换，展开/重折叠，疏水相互作用层析，第二次阳离子交换和反相色谱法。