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公开(公告)号：US07193042B1
公开(公告)日：2007-03-20
申请号：US08477984
申请日：1995-06-07
申请人： Cynthia Cowgill , Luis Juarbe-Osorio , Patricio Riquelme , Glenn Dorin , Christopher M. Bussineau , Robert D. Kudrna , Carl Scandella , Russell A. Brierley , Joan N. Abrams , John M. Hanson , Francis C. Maslanka
发明人： Cynthia Cowgill , Luis Juarbe-Osorio , Patricio Riquelme , Glenn Dorin , Christopher M. Bussineau , Robert D. Kudrna , Carl Scandella , Russell A. Brierley , Joan N. Abrams , John M. Hanson , Francis C. Maslanka
CPC分类号： C07K14/65
摘要： Methods for purifying authentic, properly folded IGF polypeptides from yeast transformants are disclosed. The methods include a refolding step and provide for high yields of authentic IGF from a variety of yeast strains.
摘要翻译： 公开了从酵母转化体纯化正确的，适当折叠的IGF多肽的方法。 所述方法包括重折叠步骤，并提供来自各种酵母菌株的高产量的正确的IGF。
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公开(公告)号：US07071313B1
公开(公告)日：2006-07-04
申请号：US09564414
申请日：2000-05-02
申请人： Cynthia Cowgill , Luis Juarbe-Osorio , Patricio Riquelme , Glenn Dorin , Christopher M. Bussineau , Robert D Kudrna , Asuman G. Ozturk , Carl Scandella , Russell A. Brierley , Joan N. Abrams , John M. Hanson , Francis C. Maslanka
发明人： Cynthia Cowgill , Luis Juarbe-Osorio , Patricio Riquelme , Glenn Dorin , Christopher M. Bussineau , Robert D Kudrna , Asuman G. Ozturk , Carl Scandella , Russell A. Brierley , Joan N. Abrams , John M. Hanson , Francis C. Maslanka
IPC分类号： C07K1/18
CPC分类号： C07K14/65
摘要： Methods for purifying authentic, properly folded IGF polypeptides from yeast transformants are disclosed. The methods include a refolding step and provide for high yields of authentic IGF from a variety of yeast strains.
摘要翻译： 公开了从酵母转化体纯化正确的，适当折叠的IGF多肽的方法。 所述方法包括重折叠步骤，并提供来自各种酵母菌株的高产量的正确的IGF。
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公开(公告)号：US06756484B1
公开(公告)日：2004-06-29
申请号：US09614947
申请日：2000-07-12
申请人： Russell A. Brierley , Joan N. Abrams , John M. Hanson , Francis C. Maslanka , Cynthia Cowgill , Luis G. Juarbe-Osorio , Patricio Riquelme , Glenn Dorin
发明人： Russell A. Brierley , Joan N. Abrams , John M. Hanson , Francis C. Maslanka , Cynthia Cowgill , Luis G. Juarbe-Osorio , Patricio Riquelme , Glenn Dorin
IPC分类号： C07K14475
CPC分类号： C07K14/65 , G01N30/02 , B01D15/422
摘要： Disclosed is an improved process for obtaining purified, monomeric, intact, correctly-folded insulin-like growth factor-I (also known as somatomedin-C). The improvements, consisting primarily of the addition of an IGF-I unfolding/refolding step and the substitution of a reverse phase chromatography step for a gel filtration chromatography step result in a three-fold increase in final yield. The process includes the following steps, in order: first cation exchange, unfolding/refolding, hydrophobic interaction chromatography, second cation exchange, and reverse phase chromatography.
摘要翻译： 公开了用于获得纯化的，单体的，完整的，正确折叠的胰岛素样生长因子-I（也称为生长因子C）的改进方法。 主要由IGF-I展开/重折叠步骤的添加和用于凝胶过滤层析步骤的反相色谱步骤的替代的改进导致最终产率增加三倍。 该方法包括以下步骤：首先进行阳离子交换，展开/重折叠，疏水相互作用层析，第二次阳离子交换和反相色谱法。
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公开(公告)号：US6117983A
公开(公告)日：2000-09-12
申请号：US990490
申请日：1997-12-15
申请人： Cynthia Cowgill , Louis Juarbe , Patricio Riquelme , Glenn Dorin , Christopher M. Bussineau , Robert D. Kudrna , Ausman G. Otzurk
发明人： Cynthia Cowgill , Louis Juarbe , Patricio Riquelme , Glenn Dorin , Christopher M. Bussineau , Robert D. Kudrna , Ausman G. Otzurk
IPC分类号： C12N15/09 , C07K1/113 , C07K14/65 , C12P21/00 , C12P21/02 , C12R1/84 , C07K1/18 , C07K1/22 , C07K14/475
CPC分类号： C07K1/1133 , C07K14/65
摘要： Methods for purifying authentic, properly folded IGF polypeptides from yeast transformants are disclosed. The methods include a refolding step and provide for high yields of authentic IGF from a variety of yeast strains.
摘要翻译： 公开了从酵母转化体纯化正确的，适当折叠的IGF多肽的方法。 所述方法包括重折叠步骤，并提供来自各种酵母菌株的高产量的正确的IGF。
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公开(公告)号：US20050037475A1
公开(公告)日：2005-02-17
申请号：US10753078
申请日：2004-01-08
申请人： David Reifsnyder , Duane Inlow , Glenn Dorin , Patricio Riquelme , Cynthia Cowgill , Douglas Bolesch , Mark Gustafson
发明人： David Reifsnyder , Duane Inlow , Glenn Dorin , Patricio Riquelme , Cynthia Cowgill , Douglas Bolesch , Mark Gustafson
CPC分类号： C07K5/1008 , A61K38/00 , C07K5/1013 , C07K14/8114
摘要： Highly purified preparations of TFPI or TFPI analogs can be prepared using a method that generally involves the following steps: (1) expression of TFPI or TFPI analog in E. coli, (2) isolation of refractile bodies, (3) dissolution of the refractile bodies and refolding of the expressed TFPI or TFPI analog, (4) SP-Sepharose fast flow (FF) chromatography, (5) a first concentration and diafiltration step, (6) Q-Sepharose high (HP) performance chromatography, (7) butyl hydrophobic interaction chromatography (HIC), (8) SP-Sepharose HP chromatography, and (9) a second concentration/diafiltration step. Less than about 12% of the TFPI or TFPI analog molecules in such preparations are modified TFPI or TFPI analog species (i.e., oxidized, carbamylated, acetylated, deamidated, aggregated, or misfolded species).
摘要翻译： TFPI或TFPI类似物的高度纯化制剂可以使用通常包括以下步骤的方法制备：（1）在大肠杆菌中表达TFPI或TFPI类似物，（2）分离折射体，（3）折射体的溶解 （4）SP-Sepharose快速流动（FF）色谱法，（5）第一次浓缩和渗滤步骤，（6）Q-Sepharose高（HP）性能色谱法，（7）表征TFPI或TFPI类似物的体内和重折叠，（4） 丁基疏水相互作用层析（HIC），（8）SP-Sepharose HP层析，和（9）第二次浓缩/渗滤步骤。 在这种制剂中，小于约12％的TFPI或TFPI类似物分子是修饰的TFPI或TFPI类似物（即氧化的，氨基甲酰化的，乙酰化的，脱酰胺的，聚集的或错误折叠的物种）。
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公开(公告)号：US20050131213A1
公开(公告)日：2005-06-16
申请号：US10939239
申请日：2004-09-09
申请人： Patricio Riquelme , Corazon Victa , Walter Crosier , John Wendell
发明人： Patricio Riquelme , Corazon Victa , Walter Crosier , John Wendell
IPC分类号： C07K14/54 , C07K14/56 , C07K14/565
CPC分类号： C07K14/565
摘要： The present invention is directed to the purification and commercialization of therapeutic polypeptides that have. In one aspect of the invention, a method of purifying Interferon β-1b using size exclusion chromatography is provided. In another aspect of the invention, a method of purifying a polypeptide using size chromatography is provided. A third aspect provides a method of commercializing a polypeptide.
摘要翻译： 本发明涉及治疗多肽的纯化和商业化。 在本发明的一个方面，提供了使用尺寸排阻色谱法纯化干扰素β-1b的方法。 在本发明的另一方面，提供了使用大小色谱法纯化多肽的方法。 第三方面提供了使多肽商业化的方法。
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