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公开(公告)号：US20240230406A1
公开(公告)日：2024-07-11
申请号：US18393636
申请日：2023-12-21
发明人： Fan-Ching Chien , Kun-Yu Lai , Ching-Lung Luo
CPC分类号： G01J3/2823 , G01J3/0208 , G01J3/021 , G01J3/027 , G01J3/06 , G01J3/10 , G01J3/18 , G01J2003/064 , G01J2003/069 , G01J2003/1213 , G01J2003/2826
摘要： The present invention provides a wide-filed spectral imaging system including a laser generator, a wavelength adjustment module, an objective lens, and a single-pixel imaging and a spectral separating module. The laser generator is configured to generate a laser excitation beam. The wavelength adjustment module is configured to disperse the laser excitation beam into a plurality of beams of different wavelengths. The objective lens is configured to focus the plurality of beams of different wavelengths on a sample to excite molecules under test in the sample and generate an emission light. The single-pixel imaging and spectral separating module is configured to generate a series of patterns and modulate the emission light with the series of patterns to generate a diffracted beam. The single-pixel imaging and spectral separating module further disperses the wavelength of the diffracted beam, collects light signals of the expanded diffracted beam, and performs a spectral image reconstruction.
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公开(公告)号：US10994993B2
公开(公告)日：2021-05-04
申请号：US16547607
申请日：2019-08-22
发明人： Fan-Ching Chien , Chun-Yu Lin
摘要： A method of forming an enhanced super-resolution image is provided. The method includes: preparing a substrate comprising a glass substrate, a metal layer on the glass substrate, and a biolayer on the metal layer; placing a biological specimen on the substrate, the biological specimen being in contact with the biolayer and being attached to the metal layer through the biolayer, in which the biological specimen is labeled by a plurality of spontaneous blinking elements therein; irradiating a light beam to the metal layer via the glass substrate; receiving fluorescence signals emitted from the spontaneous blinking elements within a time period; fitting a plurality of functions respectively to each of the fluorescence signals; pinpointing peak positions of the functions; and reconstructing the peak positions to derive the enhanced super-resolution image of an underlying structure of the biological specimen in proximity to the metal layer.
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公开(公告)号：US10295469B2
公开(公告)日：2019-05-21
申请号：US15897446
申请日：2018-02-15
发明人： Fan-Ching Chien , Chi-Hsiang Lien
摘要： The invention provides a temporal focusing-based multiphoton excitation fluorescence microscopy system capable of tunable-wavelength excitation and an excitation wavelength selection module thereof. The temporal focusing-based multiphoton excitation fluorescence microscopy system comprises: an excitation light generating module for generating excitation light; an excitation wavelength selection module for generating reflected light having a predetermined output angle in accordance with the wavelength of the excitation light and generating detecting excitation light through a diffraction unit; and a fluorescent microscope. With the practice of the present invention, the wavelength of the excitation light can be changed by the excitation wavelength selection module in real time to excite different fluorophores with the corresponding maximum multiphoton absorption wavelengths, so as to obtain better fluorescent signals and improve the image quality of the temporal focusing-based multiphoton excitation fluorescence microscope system.
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公开(公告)号：US10976577B1
公开(公告)日：2021-04-13
申请号：US16691560
申请日：2019-11-21
发明人： Kun-Yu Lai , Fan-Ching Chien
摘要： A sensing substrate including a substrate, a quantum well structure, a sensing surface and metal nanoparticles is provided. The quantum well structure is disposed on the substrate, and the quantum well structure includes at least one first metal nitride layer and second metal nitride layers. The first metal nitride layers and the second metal nitride layers are stacked on the substrate in alternation manner. The quantum well structure is located between the sensing surface and the substrate. The metal nanoparticles are disposed on the sensing surface, and the sensing surface is a rough surface. A manufacturing method of the sensing substrate and a sensor are also provided.
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