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公开(公告)号：US20090136938A1
公开(公告)日：2009-05-28
申请号：US12135564
申请日：2008-06-09
申请人： Nengbing Tao , Zoe McCuddin , Stanton Dotson , Fenggao Dong , Frederic Achard , Sam Eathington
发明人： Nengbing Tao , Zoe McCuddin , Stanton Dotson , Fenggao Dong , Frederic Achard , Sam Eathington
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6895 , A01H1/04 , C12Q1/6827 , C12Q1/6874 , C12Q2600/13 , C12Q2600/156 , G16B30/00 , G16B35/00 , G16C20/60
摘要： The present invention provides breeding methods and compositions to enhance the germplasm of a plant by the use of direct nucleic acid sequence information. The methods describe the identification and accumulation of preferred nucleic acid sequences in the germplasm of a breeding population of plants.
摘要翻译： 本发明提供了通过使用直接核酸序列信息来增强植物种质的育种方法和组合物。 该方法描述了植物繁殖群体种质中优选核酸序列的鉴定和积累。
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公开(公告)号：US20130123113A1
公开(公告)日：2013-05-16
申请号：US13247528
申请日：2011-09-28
申请人： Nengbing Tao , Zoe McCuddin , Stanton Dotson , Fenggao Dong , Frederic Achard , Sam Eathington
发明人： Nengbing Tao , Zoe McCuddin , Stanton Dotson , Fenggao Dong , Frederic Achard , Sam Eathington
IPC分类号： C12Q1/68
摘要： The present invention provides breeding methods and compositions to enhance the germplasm of a plant by the use of direct nucleic acid sequence information. The methods describe the identification and accumulation of preferred nucleic acid sequences in the germplasm of a breeding population of plants.
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公开(公告)号：US20110010102A1
公开(公告)日：2011-01-13
申请号：US12640223
申请日：2010-03-04
申请人： Nengbing Tao , Zoe McCuddin , Stanton Dotson , Sam Eathington , Frederic Achard , Fenggao Dong
发明人： Nengbing Tao , Zoe McCuddin , Stanton Dotson , Sam Eathington , Frederic Achard , Fenggao Dong
CPC分类号： A01H1/04 , C12Q1/6895
摘要： The present invention provides breeding methods and compositions to enhance the germplasm of a plant by the use of direct nucleic acid sequence information. The methods describe the identification and accumulation of preferred nucleic acid sequences in the germplasm of a breeding population of plants.
摘要翻译： 本发明提供了通过使用直接核酸序列信息来增强植物种质的育种方法和组合物。 该方法描述了植物繁殖群体种质中优选核酸序列的鉴定和积累。
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4.发明授权Automated systems for removing tissue samples from seeds, and related methods 有权用于从种子中去除组织样品的自动化系统及相关方法公开(公告)号：US09003696B2
公开(公告)日：2015-04-14
申请号：US13186126
申请日：2011-07-19
申请人： Kevin L. Deppermann , Michael W. Petersen , Allen N. Ondes , David W. Finley , William M. Fischer , John M. Jensen , David Butruille , Stanton Dotson , Sam Eathington , Heather M. Forbes , Bruce Schnicker , John Tamulonis
发明人： Kevin L. Deppermann , Michael W. Petersen , Allen N. Ondes , David W. Finley , William M. Fischer , John M. Jensen , David Butruille , Stanton Dotson , Sam Eathington , Heather M. Forbes , Bruce Schnicker , John Tamulonis
CPC分类号： G01N35/00584 , A01C1/00 , A01C1/025 , G01N1/04 , G01N1/20 , G01N1/286 , G01N33/0098 , G01N33/5097 , G01N2001/002
摘要： A seed sampling system is provided having an automated seed loading assembly including a seed bin and being operable to singulate seeds from a plurality of seeds within the seed bin. The system also includes an automated seed sampling assembly operable to remove tissue samples from the singulated seeds, and an automated seed transport assembly operable to transfer the singulated seeds from the seed loading assembly to the seed sampling assembly. The seed transport assembly includes multiple retention members. Each of the retention members is movable relative to the seed loading assembly and to the seed sampling assembly. The seed transport assembly is operable to position one of the multiple retention members adjacent to the seed loading assembly for engaging one of the singulated seeds, while positioning another of the retention members adjacent to the seed sampling assembly for presenting another of the singulated seeds to the seed sampling assembly.
摘要翻译： 提供种子采样系统，其具有包括种子仓的自动种子加载组件，并且可操作以从种子仓内的多个种子中分离种子。 该系统还包括可操作以从单个种子中去除组织样本的自动种子取样组件，以及可操作以将单种化种子从种子装载组件转移到种子采样组件的自动种子运输组件。 种子运输组件包括多个保持构件。 每个保持构件可相对于种子装载组件和种子取样组件移动。 种子运输组件可操作以将多个保持构件中的一个定位成邻近种子装载组件以接合单个种子中的一个，同时将另一个保持构件定位成与种子取样组件相邻，以将另一个单个种子呈现到 种子采样总成。
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5.发明授权公开(公告)号：US08312672B2
公开(公告)日：2012-11-20
申请号：US13108762
申请日：2011-05-16
申请人： Kevin L. Deppermann , Michael W. Petersen , David Butruille , Stanton Dotson , Sam Eathington , Heather Forbes , Bruce Schnicker , John Tamulonis
发明人： Kevin L. Deppermann , Michael W. Petersen , David Butruille , Stanton Dotson , Sam Eathington , Heather Forbes , Bruce Schnicker , John Tamulonis
CPC分类号： A01H1/02 , A01H1/04 , A01H1/08 , A01H5/10 , C12N15/8241 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156
摘要： The present invention provides for novel methods to facilitate germplasm improvement activities through the use of high throughput, nondestructive sampling of seeds. A method for analyzing a population of haploid seeds generally includes providing a population of seeds comprising haploid seeds, removing tissue samples from a plurality of the seeds in the population using an automated seed sampler system while preserving the germination viability of the sampled seeds, and analyzing the tissue samples for the presence or absence of one or more characteristics indicative of at least one genetic or chemical trait.
摘要翻译： 本发明提供了通过使用高通量，种子的非破坏性采样来促进种质改良活动的新方法。 用于分析单倍体种子群体的方法通常包括提供包含单倍体种子的种子群体，使用自动种子取样器系统从群体中的多个种子中去除组织样本，同时保持取样种子的发芽活力，并分析 所述组织样品存在或不存在指示至少一种遗传或化学性状的一种或多种特征。
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6.发明申请AUTOMATED SYSTEMS FOR REMOVING TISSUE SAMPLES FROM SEEDS, AND RELATED METHODS 有权用于从种子中去除组织样品的自动化系统及相关方法公开(公告)号：US20120117865A1
公开(公告)日：2012-05-17
申请号：US13186126
申请日：2011-07-19
申请人： Kevin L. Deppermann , Michael W. Petersen , Allen N. Ondes , David W. Finley , William M. Fischer , John M. Jensen , David Butruille , Stanton Dotson , Sam Eathington , Heather M. Forbes , Bruce Schnicker , John Tamulonis
发明人： Kevin L. Deppermann , Michael W. Petersen , Allen N. Ondes , David W. Finley , William M. Fischer , John M. Jensen , David Butruille , Stanton Dotson , Sam Eathington , Heather M. Forbes , Bruce Schnicker , John Tamulonis
IPC分类号： A01C1/00
CPC分类号： G01N35/00584 , A01C1/00 , A01C1/025 , G01N1/04 , G01N1/20 , G01N1/286 , G01N33/0098 , G01N33/5097 , G01N2001/002
摘要： A seed sampling system is provided having an automated seed loading assembly including a seed bin and being operable to singulate seeds from a plurality of seeds within the seed bin. The system also includes an automated seed sampling assembly operable to remove tissue samples from the singulated seeds, and an automated seed transport assembly operable to transfer the singulated seeds from the seed loading assembly to the seed sampling assembly. The seed transport assembly includes multiple retention members. Each of the retention members is movable relative to the seed loading assembly and to the seed sampling assembly. The seed transport assembly is operable to position one of the multiple retention members adjacent to the seed loading assembly for engaging one of the singulated seeds, while positioning another of the retention members adjacent to the seed sampling assembly for presenting another of the singulated seeds to the seed sampling assembly.
摘要翻译： 提供种子采样系统，其具有包括种子仓的自动种子加载组件，并且可操作以从种子仓内的多个种子中分离种子。 该系统还包括可操作以从单个种子中去除组织样本的自动种子取样组件，以及可操作以将单种化种子从种子装载组件转移到种子采样组件的自动种子运输组件。 种子运输组件包括多个保持构件。 每个保持构件可相对于种子装载组件和种子取样组件移动。 种子运输组件可操作以将多个保持构件中的一个定位成与种子装载组件相邻以接合单个种子之一，同时将另一个保持构件定位成与种子取样组件相邻，以将另一个单个种子呈现到 种子采样总成。
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7.发明申请公开(公告)号：US20110217700A1
公开(公告)日：2011-09-08
申请号：US13108762
申请日：2011-05-16
申请人： Kevin L. Deppermann , Michael W. Petersen , David Butruille , Stanton Dotson , Sam Eathington , Heather Forbes , Bruce Schnicker , John Tamulonis
发明人： Kevin L. Deppermann , Michael W. Petersen , David Butruille , Stanton Dotson , Sam Eathington , Heather Forbes , Bruce Schnicker , John Tamulonis
CPC分类号： A01H1/02 , A01H1/04 , A01H1/08 , A01H5/10 , C12N15/8241 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156
摘要： The present invention provides for novel methods to facilitate germplasm improvement activities through the use of high throughput, nondestructive sampling of seeds. A method for analyzing a population of haploid seeds generally includes providing a population of seeds comprising haploid seeds, removing tissue samples from a plurality of the seeds in the population using an automated seed sampler system while preserving the germination viability of the sampled seeds, and analyzing the tissue samples for the presence or absence of one or more characteristics indicative of at least one genetic or chemical trait.
摘要翻译： 本发明提供了通过使用高通量，种子的非破坏性采样来促进种质改良活动的新方法。 用于分析单倍体种子群体的方法通常包括提供包含单倍体种子的种子群体，使用自动种子取样器系统从群体中的多个种子中去除组织样本，同时保持取样种子的发芽活力，并分析 所述组织样品存在或不存在指示至少一种遗传或化学性状的一种或多种特征。
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8.发明授权公开(公告)号：US07941969B2
公开(公告)日：2011-05-17
申请号：US12767640
申请日：2010-04-26
申请人： Kevin L. Deppermann , Michael W. Petersen , David Butruille , Stanton Dotson , Sam Eathington , Heather Forbes , Bruce Schnicker , John Tamulonis
发明人： Kevin L. Deppermann , Michael W. Petersen , David Butruille , Stanton Dotson , Sam Eathington , Heather Forbes , Bruce Schnicker , John Tamulonis
CPC分类号： A01H1/02 , A01H1/04 , A01H1/08 , A01H5/10 , C12N15/8241 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156
摘要： The present invention provides for novel methods to facilitate germplasm improvement activities through the use of high throughput, nondestructive sampling of seeds. A method for analyzing a population of haploid seeds generally includes providing a population of seeds comprising haploid seeds, removing tissue samples from a plurality of the seeds in the population using an automated seed sampler system while preserving the germination viability of the sampled seeds, and analyzing the tissue samples for the presence or absence of one or more characteristics indicative of at least one genetic or chemical trait.
摘要翻译： 本发明提供了通过使用高通量，种子的非破坏性采样来促进种质改良活动的新方法。 用于分析单倍体种子群体的方法通常包括提供包含单倍体种子的种子群体，使用自动种子取样器系统从群体中的多个种子中去除组织样本，同时保持取样种子的发芽活力，并分析 所述组织样品存在或不存在指示至少一种遗传或化学性状的一种或多种特征。
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9.发明申请公开(公告)号：US20100263087A1
公开(公告)日：2010-10-14
申请号：US12767640
申请日：2010-04-26
申请人： Kevin L. Deppermann , Michael W. Petersen , David Butruille , Stanton Dotson , Sam Eathington , Heather Forbes , Bruce Schnicker , John Tamulonis
发明人： Kevin L. Deppermann , Michael W. Petersen , David Butruille , Stanton Dotson , Sam Eathington , Heather Forbes , Bruce Schnicker , John Tamulonis
CPC分类号： A01H1/02 , A01H1/04 , A01H1/08 , A01H5/10 , C12N15/8241 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156
摘要： The present invention provides for novel methods to facilitate germplasm improvement activities through the use of high throughput, nondestructive sampling of seeds. A method for analyzing a population of haploid seeds generally includes providing a population of seeds comprising haploid seeds, removing tissue samples from a plurality of the seeds in the population using an automated seed sampler system while preserving the germination viability of the sampled seeds, and analyzing the tissue samples for the presence or absence of one or more characteristics indicative of at least one genetic or chemical trait.
摘要翻译： 本发明提供了通过使用高通量，种子的非破坏性采样来促进种质改良活动的新方法。 用于分析单倍体种子群体的方法通常包括提供包含单倍体种子的种子群体，使用自动种子取样器系统从群体中的多个种子中去除组织样本，同时保持取样种子的发芽活力，并分析 所述组织样品存在或不存在指示至少一种遗传或化学性状的一种或多种特征。
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10.发明授权公开(公告)号：US07703238B2
公开(公告)日：2010-04-27
申请号：US11680611
申请日：2007-02-28
申请人： Kevin L. Deppermann , Michael W. Petersen , David Butruille , Stanton Dotson , Sam Eathington , Heather Forbes , Bruce Schnicker , John Tamulonis
发明人： Kevin L. Deppermann , Michael W. Petersen , David Butruille , Stanton Dotson , Sam Eathington , Heather Forbes , Bruce Schnicker , John Tamulonis
CPC分类号： A01H1/02 , A01H1/04 , A01H1/08 , A01H5/10 , C12N15/8241 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156
摘要： The present invention provides for novel methods to facilitate germplasm improvement activities through the use of high throughput, nondestructive sampling of seeds. In one embodiment, a high-throughput, non-destructive method for analyzing individual seeds in a population of seeds comprises removing a sample from a plurality of seeds in the population while preserving the germination viability of the seed and analyzing the sample for the presence or absence of one or more characteristics of at least one genetic or chemical trait.
摘要翻译： 本发明提供了通过使用高通量，种子的非破坏性采样来促进种质改良活动的新方法。 在一个实施方案中，用于分析种子群体中的个体种子的高通量，非破坏性方法包括从群体中的多个种子中除去样品，同时保持种子的发芽活力并分析样品的存在或 不存在至少一种遗传或化学性状的一个或多个特征。
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