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    Chimeric gene for the transformation of plants
    1.
    发明授权
    Chimeric gene for the transformation of plants 失效
    用于植物转化的嵌合基因

    公开(公告)号：US06362396B1

    公开(公告)日：2002-03-26

    申请号：US09013520

    申请日：1998-01-26

    申请人： Nicole Chaubet ,  Claude Gigot ,  Georges Freyssinet ,  Bernard Leroux ,  Michel Lebrun ,  Alain Sailland

    发明人： Nicole Chaubet ,  Claude Gigot ,  Georges Freyssinet ,  Bernard Leroux ,  Michel Lebrun ,  Alain Sailland

    IPC分类号： A01H500

    CPC分类号： C12N9/1092 ,  C12N15/8274

    摘要： 1) Chimeric gone for conferring to plants an increased tolerance to a herbicide. 2) It comprises, in the direction of transcription, a promoter region, a transit peptide region, a sequence encoding glyphosate tolerance and a polyadenylation signal region, wherein the promoter region consists of at least one promoter of a plant histone gene enabling the expression of the herbicide tolerance protein in the regions of glyphosate accumulation. 3) Production of glyphosate-tolerant plants.

    摘要翻译： 1）嵌合去除赋予植物增加对除草剂的耐受性。 2）其在转录方向包含启动子区，转运肽区，编码草甘膦耐受的序列和多聚腺苷酸化信号区，其中启动子区由植物组蛋白基因的至少一个启动子组成，使得能够表达 草甘膦积累区域的除草剂耐受性蛋白。 3）耐草甘膦植物的生产。

    Mutated 5-enolpyruvylshikimate-3-phosphate synthase, gene coding for said protein and transformed plants containing said gene
    2.
    发明授权
    Mutated 5-enolpyruvylshikimate-3-phosphate synthase, gene coding for said protein and transformed plants containing said gene 失效
    突变的5-烯醇丙酮酸莽草酸-3-磷酸合酶，编码所述蛋白质的基因和含有所述基因的转化植物

    公开(公告)号：US06566587B1

    公开(公告)日：2003-05-20

    申请号：US08945144

    申请日：1998-01-20

    申请人： Michel Lebrun ,  Alain Sailland ,  Georges Freyssinet ,  Eric Degryse

    发明人： Michel Lebrun ,  Alain Sailland ,  Georges Freyssinet ,  Eric Degryse

    IPC分类号： C12N1582

    CPC分类号： C12N9/1092 ,  C12N15/8275

    摘要： The present invention relates to DNA molecules of plant origin encoding a modified 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) enzyme wherein the a first EPSPS coding sequence that normally encodes a threonine residue of a mature EPSPS sequence is modified to encode isoleucine of the mature EPSPS sequence, and a second EPSPS coding sequence that normally encodes a proline residue of a mature EPSPS sequence is modified to encode serine of the mature EPSPS sequence, wherein the first and second residues are respectively located at relatively positions 102 and 106 of a mature EPSPS sequence encoded by said DNA molecule; and the production of a transgenic plant resistant or tolerant to a herbicide of the phosphonomethylglycine family, e.g., glyphosate. The mutated enzyme, which substantially maintains the catalytic activity of the wild-type enzyme, allows for increased tolerance or tolerance of the plant to a herbicide of the phosphonomethylglycine family, and allows for the substantially normal growth or development of the plant, its organs, tissues or cells.

    摘要翻译： 本发明涉及编码经修饰的5-烯醇丙酮酸莽草酸-3-磷酸合成酶（EPSPS）酶的植物来源的DNA分子，其中通常将编码成熟EPSPS序列的苏氨酸残基的第一EPSPS编码序列修饰为编码 修饰成熟的EPSPS序列，并且通常编码成熟EPSPS序列的脯氨酸残基的第二EPSPS编码序列被修饰以编码成熟EPSPS序列的丝氨酸，其中第一和第二残基分别位于成熟的EPSPS序列的相对位置102和106 由所述DNA分子编码的EPSPS序列; 以及产生对膦酰基甲基甘氨酸家族例如草甘膦的除草剂具有抗性或耐受性的转基因植物。 基本上保持野生型酶的催化活性的突变酶允许植物对膦酰基甲基甘氨酸家族的除草剂的耐受性或耐受性增加，并允许植物，其器官的基本正常生长或发育， 组织或细胞。

    Chimeric gene for the transformation of plants
    3.
    发明授权
    Chimeric gene for the transformation of plants 失效
    用于植物转化的嵌合基因

    公开(公告)号：US5510471A

    公开(公告)日：1996-04-23

    申请号：US251621

    申请日：1994-05-31

    申请人： Michel Lebrun ,  Bernard Leroux ,  Alain Sailland

    发明人： Michel Lebrun ,  Bernard Leroux ,  Alain Sailland

    IPC分类号： A01H1/00 ,  A01H5/00 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  C12N15/30 ,  C12N15/54 ,  C12N15/62 ,  C12N15/82 ,  C12R1/01 ,  C12R1/91 ,  C07H17/00 ,  C12N15/00 ,  C12N15/29

    CPC分类号： C12N15/62 ,  C12N15/8275 ,  C07K2319/08

    摘要： Chimeric gene for conferring to plants an increased tolerance to a herbicide having as its target EPSPS comprises, in the direction of transcription, a promoter region, a transit peptide region, a coding sequence for glyphosate tolerance and a polyadenylation signal region, wherein the transit peptide region comprises, in the direction of translation, at least one transit peptide of a plant gene encoding a plastid-localised enzyme, a partial sequence of the N-terminal mature part of a plant gene encoding a plastid-localised enzyme and then a second transit peptide of a plant gene encoding a plastid- localised enzyme. Production of glyphosate-tolerant plants is disclosed.

    摘要翻译： 用于赋予植物对具有作为其目标EPSPS的除草剂的增加的耐受性的嵌合基因包括在转录方向上的启动子区，转运肽区，草甘膦耐受的编码序列和多聚腺苷酸化信号区，其中转运肽 翻译方向包括编码质体定位酶的植物基因的至少一个转运肽，编码质体定位酶的植物基因的N末端成熟部分的部分序列，然后是第二转运 编码质体定位酶的植物基因的肽。 公开了耐草甘膦植物的生产。

    Chimeric gene for the transformation of plants
    4.
    再颁专利
    Chimeric gene for the transformation of plants 失效
    用于植物转化的嵌合基因

    公开(公告)号：USRE37287E1

    公开(公告)日：2001-07-17

    申请号：US09025042

    申请日：1998-02-17

    申请人： Michel Lebrun ,  Bernard Leroux ,  Alain Sailland

    发明人： Michel Lebrun ,  Bernard Leroux ,  Alain Sailland

    IPC分类号： A01H500

    CPC分类号： C12N15/62 ,  C07K2319/08 ,  C12N15/8275

    摘要： Chimeric gene for conferring to plants an increased tolerance to a herbicide having as its target EPSPS comprises, in the direction of transcription, a promoter region, a transit peptide region, a coding sequence for glyphosate tolerance and a polyandenylation signal region, wherein the transit peptide region comprises, in the direction of translation, at least one transit peptide of a plant gene encoding a plastid-localized enzyme and then a second transit peptide of a plant gene encoding, a plastid-localized enzyme. Production of glyphosate-tolerant plants is disclosed.

    摘要翻译： 用于赋予植物对具有作为其目标EPSPS的除草剂的增加的耐受性的嵌合基因包括在转录方向上的启动子区，转运肽区，草甘膦耐受的编码序列和多聚腺苷酸化信号区，其中转运肽 在翻译方向上包含编码质体定位酶的植物基因的至少一个转运肽，然后编码质体定位的酶的植物基因的第二转运肽。 公开了耐草甘膦植物的生产。

    Chimeric gene for the transformation of plants
    5.
    再颁专利
    Chimeric gene for the transformation of plants 失效
    用于植物转化的嵌合基因

    公开(公告)号：USRE36449E

    公开(公告)日：1999-12-14

    申请号：US25082

    申请日：1998-02-17

    申请人： Michel Lebrun ,  Bernard Leroux ,  Alain Sailland

    发明人： Michel Lebrun ,  Bernard Leroux ,  Alain Sailland

    IPC分类号： C12N15/62 ,  C12N15/82 ,  A01H5/00 ,  C07H17/00 ,  C12N15/00 ,  C12N15/29

    CPC分类号： C12N15/8275 ,  C12N15/62 ,  C07K2319/08

    摘要： Chimeric gene for conferring to plants an increased tolerance to a herbicide having as its target EPSPS comprises, in the direction of transcription, a promoter region, a transit peptide region, a coding sequence for glyphosate tolerance and a polyadenylation signal region, wherein the transit peptide region comprises, in the direction of translation, at least one transit peptide of a plant gene encoding a plastid-localised enzyme, a partial sequence of the N-terminal mature part of a plant gene encoding a plastid-localised enzyme and then a second transit peptide of a plant gene encoding a plastid-localised enzyme. Production of glyphosate-tolerant plants is disclosed.

    摘要翻译： 用于赋予植物对具有作为其目标EPSPS的除草剂的增加的耐受性的嵌合基因包括在转录方向上的启动子区，转运肽区，草甘膦耐受的编码序列和多聚腺苷酸化信号区，其中转运肽 翻译方向包括编码质体定位酶的植物基因的至少一个转运肽，编码质体定位酶的植物基因的N末端成熟部分的部分序列，然后是第二转运 编码质体定位酶的植物基因的肽。 公开了耐草甘膦植物的生产。

    Chimeric gene for the transformation of plants
    6.
    发明授权
    Chimeric gene for the transformation of plants 失效
    用于植物转化的嵌合基因

    公开(公告)号：US5633448A

    公开(公告)日：1997-05-27

    申请号：US477581

    申请日：1995-06-07

    申请人： Michel Lebrun ,  Bernard Leroux ,  Alain Sailland

    发明人： Michel Lebrun ,  Bernard Leroux ,  Alain Sailland

    IPC分类号： A01H1/00 ,  A01H5/00 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  C12N15/30 ,  C12N15/54 ,  C12N15/62 ,  C12N15/82 ,  C12R1/01 ,  C12R1/91 ,  C12N15/12

    CPC分类号： C12N15/62 ,  C12N15/8275 ,  C07K2319/08

    摘要： Chimeric gene for conferring to plants an increased tolerance to a herbicide having as its target EPSPS comprises, in the direction of transcription, a promoter region, a transit peptide region, a coding sequence for glyphosate tolerance and a polyandenylation signal region, wherein the transit peptide region comprises, in the direction of translation, at least one transit peptide of a plant gene encoding a plastid-localized enzyme and then a second transit peptide of a plant gene encoding a plastid-localized enzyme. Production of glyphosate-tolerant plants is disclosed.

    摘要翻译： 用于赋予植物对具有作为其目标EPSPS的除草剂的增加的耐受性的嵌合基因包括在转录方向上的启动子区，转运肽区，草甘膦耐受的编码序列和多聚腺苷酸化信号区，其中转运肽 在翻译方向上包含编码质体定位酶的植物基因的至少一个转运肽，然后编码质体定位的酶的植物基因的第二转运肽。 公开了耐草甘膦植物的生产。