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    Method of drug loading in liposomes by gradient
    1.
    发明申请
    Method of drug loading in liposomes by gradient 审中-公开
    通过梯度在脂质体中加载药物的方法

    公开(公告)号：US20100119590A1

    公开(公告)日：2010-05-13

    申请号：US12501606

    申请日：2009-07-13

    申请人： Ning Hu ,  Gerard M. Jensen ,  Michele Sulivan ,  Stephanie Yang

    发明人： Ning Hu ,  Gerard M. Jensen ,  Michele Sulivan ,  Stephanie Yang

    IPC分类号： A61K9/127 ,  A61K31/55 ,  A61P35/00

    CPC分类号： A61K9/127 ,  A61K9/1277 ,  A61K9/1278 ,  A61K31/137 ,  A61K31/55 ,  A61K31/704

    摘要： A method for encapsulation of pharmaceutical agents (e.g., antineoplastic agents) in liposomes is provided, having preferably a high drug:lipid ratio. Liposomes can be made by a process that loads the drug by an active mechanism using a transmembrane pH gradient. Using this technique, trapping efficiencies approach 100%. Drug:lipid ratios employed are higher than for older traditional liposome preparations, and the release rate of the drug from the liposomes is reduced. After loading, residual acid is quenched with a quenching agent that is base permeable at low temperatures. The residual aciditiy is thus reduced and chemical stability (e.g. against hydrolysis) is enhanced. The stability of both the liposome and the pharmaceutical agent is thus maintained, prior to administration. The pH gradient is, however, present when the liposome is administered in vivo because the quenching agent rapidly exits the liposome.

    摘要翻译： 提供了在脂质体中封装药物（例如抗肿瘤剂）的方法，其优选具有高的药物：脂质比。 可以通过使用跨膜pH梯度的活性机制加载药物的方法制备脂质体。 使用这种技术，捕获效率接近100％。 药物：使用的脂质比例高于较老的传统脂质体制剂，并且药物从脂质体的释放速率降低。 加载后，残留的酸用低温基本可渗透的淬火剂骤冷。 残留的酸性因此降低，并且化学稳定性（例如抗水解）得到增强。 因此在施用之前维持脂质体和药剂的稳定性。 然而，当脂质体在体内施用时，pH梯度存在，因为猝灭剂快速离开脂质体。

    LIPID-BASED DISPERSIONS USEFUL FOR DRUG DELIVERY
    2.
    发明申请
    LIPID-BASED DISPERSIONS USEFUL FOR DRUG DELIVERY 审中-公开
    用于药物递送的基于脂质的分散剂

    公开(公告)号：US20090060998A1

    公开(公告)日：2009-03-05

    申请号：US10585915

    申请日：2005-01-14

    申请人： Ning Hu ,  Gerard M. Jensen ,  Stephanie Yang ,  Chiang Su-Ming

    发明人： Ning Hu ,  Gerard M. Jensen ,  Stephanie Yang ,  Chiang Su-Ming

    IPC分类号： A61K9/10

    CPC分类号： A61K9/127 ,  A61K9/0019

    摘要： The invention provides lipid-based dispersion comprising, a) phosphatidyl choline; b) an anionic phospholipid; optionally c) up to 1% cholesterol by weight of total lipids; and optionally d) a therapeutic agent; wherein the mean particle size measured by dynamic light scattering is less than 100 nm. The invention also provides pharmaceutical compositions comprising such a dispersion as well as methods of producing a therapeutic effect in a mammal comprising administering an effective amount of such a dispersion.

    摘要翻译： 本发明提供了脂质基分散体，其包含：a）磷脂酰胆碱; b）阴离子磷脂; 任选地c）总脂质重量高达1％的胆固醇; 和任选地d）治疗剂; 其中通过动态光散射测量的平均粒度小于100nm。 本发明还提供包含这种分散体的药物组合物以及在哺乳动物中产生治疗效果的方法，包括施用有效量的这种分散体。

    Lipid-based dispersions useful for drug delivery
    5.
    发明申请
    Lipid-based dispersions useful for drug delivery 审中-公开
    用于药物递送的基于脂质的分散体

    公开(公告)号：US20050238705A1

    公开(公告)日：2005-10-27

    申请号：US11035755

    申请日：2005-01-14

    申请人： Ning Hu ,  Gerard Jensen ,  Stephanie Yang ,  Su-Ming Chiang

    发明人： Ning Hu ,  Gerard Jensen ,  Stephanie Yang ,  Su-Ming Chiang

    IPC分类号： A61K9/107 ,  A61K9/127 ,  A61K9/51 ,  A61K47/00

    CPC分类号： A61K9/127 ,  A61K9/0019

    摘要： The invention provides lipid-based dispersion comprising comprising, a) phosphatidyl choline; b) an anionic phospholipid; optionally c) up to 1% cholesterol by weight of total lipids; and optionally d) a therapeutic agent; wherein the mean particle size measured by dynamic light scattering is less than 100 nm. The invention also provides pharmaceutical compositions comprising such a dispersion as well as methods of producing a therapeutic effect in a mammal comprising administering an effective amount of such a dispersion.

    摘要翻译： 本发明提供基于脂质的分散体，其包含：a）磷脂酰胆碱; b）阴离子磷脂; 任选地c）总脂质重量高达1％的胆固醇; 和任选地d）治疗剂; 其中通过动态光散射测量的平均粒度小于100nm。 本发明还提供包含这种分散体的药物组合物以及在哺乳动物中产生治疗效果的方法，包括施用有效量的这种分散体。

    MICRODEVICE FOR FUSING CELLS
    6.
    发明申请
    MICRODEVICE FOR FUSING CELLS 审中-公开
    用于融合细胞的MICRODEVICE

    公开(公告)号：US20130089929A1

    公开(公告)日：2013-04-11

    申请号：US13282833

    申请日：2011-10-27

    申请人： Sang-Woo Joo ,  Shizhi Qian ,  Ning Hu

    发明人： Sang-Woo Joo ,  Shizhi Qian ,  Ning Hu

    IPC分类号： C12M1/42 ,  C12N15/02

    CPC分类号： C12M23/16 ,  B01L3/502715 ,  B01L3/502761 ,  C12M35/02

    摘要： A microdevice for fusing cells, the microdevice including: a substrate; a first electrode array including a plurality of first electrodes, and disposed on the substrate; a microwell array including a plurality of microwells formed respectively at locations corresponding to the plurality of first electrodes, and disposed on the first electrode array; a second electrode disposed above the plurality of microwells, and including a microchannel having a predetermined height; inlet and outlet holes mutually spaced apart from the microchannel; and a power supply unit applying voltage to the plurality of first electrodes and the second electrode. Accordingly, a cell trapped in the microwell and a cell disposed on the microwell are aligned in a line between the first and second electrodes, and thus the two cells having different traits are smoothly fused in a one-to-one manner when an electric shock is applied to the two cells.

    摘要翻译： 用于融合细胞的微型装置，微型装置包括：底物; 包括多个第一电极的第一电极阵列，并设置在所述基板上; 微孔阵列，其包括分别形成在与所述多个第一电极对应的位置处的多个微孔，并且设置在所述第一电极阵列上; 设置在所述多个微孔上方的第二电极，并且包括具有预定高度的微通道; 与微通道相互间隔开的入口孔和出口孔; 以及向多个第一电极和第二电极施加电压的电源单元。 因此，捕获在微孔中的细胞和设置在微孔上的细胞在第一和第二电极之间的直线上排列，因此当电击时，具有不同性状的两个细胞以一对一的方式平滑地融合 被应用于两个单元格。

    MICRODEVICE FOR FUSING CELLS
    7.
    发明申请
    MICRODEVICE FOR FUSING CELLS 审中-公开
    用于融合细胞的MICRODEVICE

    公开(公告)号：US20130089931A1

    公开(公告)日：2013-04-11

    申请号：US13282874

    申请日：2011-10-27

    申请人： Sang-Woo JOO ,  Shizhi Qian ,  Ning Hu

    发明人： Sang-Woo JOO ,  Shizhi Qian ,  Ning Hu

    IPC分类号： C12N15/02 ,  C12M1/42

    CPC分类号： C12M35/02 ,  C12N15/02

    摘要： A microdevice for fusing cells including: a membrane with a plurality of pores having a diameter smaller than the smallest diameter among the first kind of cells and second kind of cells; a first chamber where the first cell is located and a second chamber where the second cell is located, wherein the membrane is disposed therebetween; a first electrode combined to the first chamber; a second electrode combined to the second chamber; and a power generator applying a voltage to the first and second electrodes. Accordingly, the first and second cells across the membrane may be arranged in a one-to-one manner between the first and second electrodes, and thus the first and second cells having different traits may be smoothly fused in a one-to-one manner when electric signals are sequentially applied thereto.

    摘要翻译： 一种用于熔融电池的微型装置，包括：具有直径小于第一种电池和第二种电池中的最小直径的多个孔的膜; 所述第一腔室位于所述第一室中，所述第二腔室位于所述第二腔室中，其中所述膜设置在所述第二室中; 组合到第一室的第一电极; 组合到第二室的第二电极; 以及向所述第一和第二电极施加电压的发电机。 因此，穿过膜的第一和第二细胞可以在第一和第二电极之间以一对一的方式布置，因此具有不同性状的第一和第二细胞可以以一对一的方式平滑地融合 当电信号依次施加到其上时。

    Blending mobile search results
    8.
    发明授权
    Blending mobile search results 有权
    混合移动搜索结果

    公开(公告)号：US08341147B2

    公开(公告)日：2012-12-25

    申请号：US13105750

    申请日：2011-05-11

    申请人： Ning Hu ,  Vida U. Ha

    发明人： Ning Hu ,  Vida U. Ha

    IPC分类号： G06F7/00

    CPC分类号： G06F17/3053 ,  G06F17/30864 ,  G06F17/30867

    摘要： Methods, systems, and apparatus, including computer program products, for blending mobile search results. A method includes receiving a search query and multiple search results. The search results each satisfy the search query and have a respective search result quality score. The search results include generic and mobile search results. The generic and mobile search results each identify a generic and mobile resource, respectively. The search result quality scores include mobile and generic search result quality scores for the mobile and generic search results, respectively. The mobile search result quality scores and the generic search result quality scores were generated according to different scoring formulas. Based on one or more terms in the search query, the search query is classified as a mobile query. As a consequence, one or more search result quality scores are modified to improve the sorting of search results that include both mobile and generic search results.

    摘要翻译： 方法，系统和设备，包括用于混合移动搜索结果的计算机程序产品。 一种方法包括接收搜索查询和多个搜索结果。 搜索结果各自满足搜索查询并具有相应的搜索结果质量得分。 搜索结果包括通用和移动搜索结果。 通用和移动搜索结果各自分别标识通用和移动资源。 搜索结果质量得分分别包括移动和通用搜索结果的移动和一般搜索结果质量得分。 根据不同的评分公式生成移动搜索结果质量得分和通用搜索结果质量得分。 基于搜索查询中的一个或多个术语，搜索查询被分类为移动查询。 因此，修改一个或多个搜索结果质量得分以改进包括移动和一般搜索结果的搜索结果的排序。