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公开(公告)号：US08178320B2
公开(公告)日：2012-05-15
申请号：US10496349
申请日：2002-11-22
CPC分类号： C40B40/02 , C07K16/00 , C07K16/005 , C07K2317/565 , C07K2317/622 , C12N15/1037 , C12N15/1093 , G01N2500/00
摘要： An artificial antibody library with a super-repertory (1011 or more) is constructed by: using a cDNA library as a template, amplifying a fragment containing the CDR1 and CDR2s regions of the VH or VL region of immunoglobulin gene and a fragment containing the CDR3 region each by the PCR method; integrating the VH library and the VL library, which are little contaminated with unexpressionable repertory and have high safety, into an non-expression vector; transferring it into a host; and then shuffling the VH region in the VH library with the VL region in the VL library.
摘要翻译： 通过使用cDNA文库作为模板，扩增含有免疫球蛋白基因的VH或VL区域的CDR1和CDR2区域的片段和含有CDR3的片段，构建具有超级片段（1011以上）的人工抗体文库 区域各自采用PCR法; 将不具有不可表达的毒素并且具有高度安全性的VH文库和VL文库整合到非表达载体中; 将其转移到主机中; 然后用VL库中的VL区域混合VH库中的VH区。
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公开(公告)号：US20060057632A1
公开(公告)日：2006-03-16
申请号：US10496349
申请日：2002-11-22
IPC分类号： C40B40/10 , G01N33/53 , A61K39/395 , C12N5/06
CPC分类号： C40B40/02 , C07K16/00 , C07K16/005 , C07K2317/565 , C07K2317/622 , C12N15/1037 , C12N15/1093 , G01N2500/00
摘要： An artificial antibody library with a super-repertory (1011 or more) is constructed by: using a cDNA library as a template, amplifying a fragment containing the CDR1 and CDR2s regions of the VH or VL region of immunoglobulin gene and a fragment containing the CDR3 region each by the PCR method; integrating the VH library and the VL library, which are little contaiminated with unexpressionable repertory and have high safety, into an nono-expression vector; transferring it into a host; and then shuffling the VH region in the VH library with the VL region in the VL library.
摘要翻译： 通过使用cDNA文库作为模板，扩增含有VH或VL区的CDR1和CDR2区的片段，构建具有超级序列（10个或更多）的人源抗体文库 免疫球蛋白基因和含有CDR3区域的片段各自通过PCR方法; 将不具有不可表达的重复序列的VH文库和VL库整合到非表达载体中; 将其转移到主机中; 然后用VL库中的VL区域混合VH库中的VH区。
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