公开(公告)号：US20240003906A1

公开(公告)日：2024-01-04

申请号：US17919270

申请日：2020-04-15

Applicant: Nordic Bioscience A/S

Inventor： Martin Pehrsson ,  Morten Asser Karsdal ,  Diana Julie Leeming ,  Mette Juul Fisker ,  Tina Manon-Jensen ,  Joachim Høg Mortensen

IPC: G01N33/68 ,  C07K16/18

CPC classification number: G01N33/6887 ,  C07K16/18 ,  G01N2333/78 ,  G01N2800/065 ,  C07K2317/34 ,  G01N2800/7052 ,  G01N2800/085 ,  G01N2470/04 ,  G01N2800/56 ,  G01N2800/52

Abstract: The present invention relates to a sandwich immunoassay and kits for detecting in a biological sample cross-linked CTX-III, and its use in evaluating the efficacy of drugs targeting lysyl oxidases (LOXs). Cross-linked CTX-III can be used as a biomarker in diseases associated with fibrosis, including liver fibrosis, chronic intestinal disease, eosinophilic esophagitis and cancer.

公开(公告)号：US20240011997A1

公开(公告)日：2024-01-11

申请号：US18358070

申请日：2023-07-25

Applicant: Nordic Bioscience A/S

Inventor： Karoline Natasja Stæhr Gudmann ,  Jannie Marie Bülow Sand ,  Tina Manon-Jensen ,  Diana Julie Leeming ,  Morten Karsdal

IPC: G01N33/577

CPC classification number: G01N33/577 ,  G01N2800/122 ,  G01N2800/52 ,  G01N2470/04

Abstract: Described herein are monoclonal antibodies, assay kits and immumoassay methods for quantifying elastin fragments with a C-terminus amino acid sequence LPGGYGLPYT (SEQ ID NO: 1) in a patient biofluid sample, and uses thereof for detecting or quantifying fibrotic diseases such as chronic obstructive lung disease (COPD).

公开(公告)号：US20160061844A1

公开(公告)日：2016-03-03

申请号：US14784512

申请日：2014-04-15

Applicant: NORDIC BIOSCIENCE A/S

Inventor： Diana Julie Leeming ,  Mette Juul Nielsen ,  Morten Karsdal

IPC: G01N33/68 ,  C07K16/18

CPC classification number: G01N33/6878 ,  C07K16/18 ,  C07K2317/34 ,  G01N2333/78 ,  G01N2800/085 ,  G01N2800/7052

Abstract: Provided is a monoclonal antibody specifically reactive with a C-terminal neo-epitope of PIIINP comprised in a C-terminal amino acid sequence CPTGXQNYSP-COOH (SEQ ID NO: 4) in which X is Gly or Pro, and where the monoclonal antibody does not recognise or bind an elongated version of the C-terminal amino acid sequence CPTGXQNYSPQZ-COOH (SEQ ID NO: 5), in which Z is absent or is one or more amino acids of the sequence of collagen type III. Also provided is a method of immunoassay for detecting in a biological sample the C-terminal neo-epitope of PIIINP generated by N-protease cleavage of intact type III procollagen, by contacting the sample with the monoclonal antibody, and determining the amount of binding of the antibody.

Abstract translation: 提供了与包含在C末端氨基酸序列CPTGXQNYSP-COOH（SEQ ID NO：4）中的PIIINP的C末端新表位特异性反应的单克隆抗体，其中X是Gly或Pro，并且其中单克隆抗体 不识别或结合长链型C-末端氨基酸序列CPTGXQNYSPQZ-COOH（SEQ ID NO：5），其中Z不存在或者是III型胶原序列的一个或多个氨基酸。 还提供了一种用于在生物样品中检测通过将样品与单克隆抗体接触并确定结合量的方法来检测在完整III型原胶原的N-蛋白酶切割产生的PIIINP的C-末端新表位的免疫测定方法 抗体。

公开(公告)号：US20230184787A1

公开(公告)日：2023-06-15

申请号：US17925005

申请日：2021-05-12

Applicant: Nordic Bioscience A/S

Inventor： Joachim Høg Mortensen ,  Dovile Sinkeviciute ,  Tina Manon-Jensen ,  Morten Asser Karsdal ,  Anne-Christine Bay-Jensen ,  Signe Holm Nielsen ,  Annika Hummersgaard Hansen ,  Jannie Marie Bülow Sand ,  Diana Julie Leeming ,  Nicholas Willumsen ,  Christina Jensen

IPC: G01N33/68 ,  C07K16/18

CPC classification number: G01N33/6893 ,  C07K16/18 ,  G01N2333/4727 ,  G01N2800/56 ,  G01N2800/7095 ,  G01N2470/10

Abstract: Disclosed herein are immunoassay methods for detecting and quantifying human neutrophil elastase (HNE) generated fragments of calprotectin in a biofluid sample; and monoclonal antibodies and assay kits for use in such methods. The methods may be used for detecting, monitoring and/or determining the status or severity of a disease characterized by or exhibiting inflammation in a patient.

公开(公告)号：US09726674B2

公开(公告)日：2017-08-08

申请号：US14784512

申请日：2014-04-15

Applicant: Nordic Bioscience A/S

Inventor： Diana Julie Leeming ,  Mette Juul Nielsen ,  Morten Karsdal

IPC: G01N33/53 ,  G01N33/68 ,  C07K16/18

CPC classification number: G01N33/6878 ,  C07K16/18 ,  C07K2317/34 ,  G01N2333/78 ,  G01N2800/085 ,  G01N2800/7052

Abstract: Provided is a monoclonal antibody specifically reactive with a C-terminal neo-epitope of PIIINP comprised in a C-terminal amino acid sequence CPTGXQNYSP-COOH (SEQ ID NO: 4) in which X is Gly or Pro, and where the monoclonal antibody does not recognize or bind an elongated version of the C-terminal amino acid sequence CPTGXQNYSPQZ-COOH (SEQ ID NO: 5), in which Z is absent or is one or more amino acids of the sequence of collagen type III. Also provided is a method of immunoassay for detecting in a biological sample the C-terminal neo-epitope of PIIINP generated by N-protease cleavage of intact type III procollagen, by contacting the sample with the monoclonal antibody, and determining the amount of binding of the antibody.

公开(公告)号：US20160077108A1

公开(公告)日：2016-03-17

申请号：US14786301

申请日：2014-04-23

Applicant: NORDIC BIOSCIENCE A/S

Inventor： Diana Julie Leeming ,  Anders Nedergaard ,  Morten Karsdal

IPC: G01N33/68 ,  C07K16/18

CPC classification number: G01N33/6887 ,  C07K16/18 ,  C07K2317/34 ,  G01N2333/78

Abstract: Provided is an antibody which binds to an epitope of collagen type VI alpha 1 comprised in the N-terminal globular domain internal amino acid sequence -ADWGQSRDAEEAISQ- (SEQ ID NO: 1). Also provided is a method of immunoassay for detecting in a biological sample an epitope comprised in the N-terminal globular domain internal amino acid sequence -ADWGQSRDAEEAISQ- (SEQ ID NO: 1) of collagen type VI alpha 1, by contacting the sample with the antibody, and determining the amount of binding of the antibody.

Abstract translation: 本发明提供了一种抗体，其与N末端球状结构域内部氨基酸序列-ADWGQSRDAEEAISQ-（SEQ ID NO：1）中包含的VI型α1胶原的表位结合。 还提供了一种用于在生物样品中检测包含在VI型α1胶原的N-末端球状结构域内部氨基酸序列-ADWGQSRDAEEAISQ-（SEQ ID NO：1）中的表位的免疫测定方法，通过将样品与 抗体，并测定抗体的结合量。