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    METHODS FOR EXTRACELLULAR VESICLE ISOLATION AND SELECTIVE REMOVAL
    1.
    发明申请
    METHODS FOR EXTRACELLULAR VESICLE ISOLATION AND SELECTIVE REMOVAL 审中-公开

    公开(公告)号：US20190078078A1

    公开(公告)日：2019-03-14

    申请号：US16190402

    申请日：2018-11-14

    申请人： Norgen Biotek Corp.

    发明人： Yousef Haj-Ahmad

    IPC分类号： C12N15/10

    摘要： Disclosed is a method for the isolation of extracellular vesicles, including exosomes, from a liquid sample, the method comprising the steps of: adjusting the pH of a liquid sample comprising extracellular vesicles to a preselected, binding pH; contacting the liquid sample with silicon carbide, wherein at the preselected, binding pH, the extracellular vesicles bind to the silicon carbide; and eluting the bound extracellular vesicles from the silicon carbide. The liquid samples can comprise bodily fluids. Further disclosed is a method for producing a liquid sample, substantially depleted of extracellular vesicles, including exosomes.

    METHODS AND KITS FOR IMPROVING GLOBAL GENE EXPRESSION ANALYSIS OF HUMAN URINE DERIVED RNA
    4.
    发明申请
    METHODS AND KITS FOR IMPROVING GLOBAL GENE EXPRESSION ANALYSIS OF HUMAN URINE DERIVED RNA 审中-公开

    公开(公告)号：US20200024642A1

    公开(公告)日：2020-01-23

    申请号：US16042282

    申请日：2018-07-23

    申请人： Norgen Biotek Corp.

    发明人： Yousef Haj-Ahmad

    IPC分类号： C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6883

    摘要： Disclosed are methods and kits for improving global gene expression analysis for a population of RNA molecules derived from a human urine sample. In an embodiment, the method comprises the step of selectively depleting miR-10a-5p fragments from the population of RNA molecules or selectively blocking miR-10a-5p fragments within the RNA population. The miR-10a-5p depleted or miR-10a-5p blocked population of RNA can be used in a variety of global gene expression analysis protocols, including next generation sequencing. In a further embodiment, the method comprises selectively depleting or blocking miR-10b-5p fragments within the RNA population. The miR-10a-5p and/or miR-10b-5p depleted or blocked populations of RNA can also be used in global gene expression analysis protocols, including next generation sequencing. The kit comprises oligonucleotide probes comprising a nucleotide sequence that is the complement to a nucleotide sequence of the miR-10a-5p and/or oligonucleotide probes comprising a nucleotide sequence that is the complement to a nucleotide sequence of miR-10b-5p.

    Methods for extracellular vesicle isolation and selective removal
    5.
    发明授权
    Methods for extracellular vesicle isolation and selective removal 有权

    公开(公告)号：US10160964B2

    公开(公告)日：2018-12-25

    申请号：US15147969

    申请日：2016-05-06

    申请人： Norgen Biotek Corp.

    发明人： Yousef Haj-Ahmad

    IPC分类号： C12N15/10

    摘要： Disclosed is a method for the isolation of extracellular vesicles, including exosomes, from a liquid sample, the method comprising the steps of: adjusting the pH of a liquid sample comprising extracellular vesicles to a preselected, binding pH; contacting the liquid sample with silicon carbide, wherein at the preselected, binding pH, the extracellular vesicles bind to the silicon carbide; and eluting the bound extracellular vesicles from the silicon carbide. The liquid samples can comprise bodily fluids. Further disclosed is a method for producing a liquid sample, substantially depleted of extracellular vesicles, including exosomes.

    METHODS AND KITS FOR SEPARATING NUCLEIC ACIDS BY SIZE
    6.
    发明申请
    METHODS AND KITS FOR SEPARATING NUCLEIC ACIDS BY SIZE 审中-公开

    公开(公告)号：US20170268047A1

    公开(公告)日：2017-09-21

    申请号：US15460489

    申请日：2017-03-16

    申请人： Norgen Biotek Corp.

    发明人： Yousef Haj-Ahmad

    IPC分类号： C12Q1/68 ,  C12N15/10

    CPC分类号： C12Q1/6811 ,  C12N15/1006 ,  C12N15/1072 ,  C12Q1/6806 ,  C12Q2521/501 ,  C12Q2525/191 ,  C12Q2535/122

    摘要： Disclosed are methods and kits for isolating nucleic acids having a size above a desired cut-off size from a nucleic acid containing sample. The method comprises combining the sample with a binding buffer, alcohol and silicon carbide to provide a binding mixture. Nucleic acids having a size above the desired cut-off size are selectively bound to the silicon carbide. The cut-off size for selective binding to the silicon carbide is determined by the alcohol concentration of the binding mixture. The bound nucleic acids are separated from the remaining sample. The bound nucleic acids are optionally washed and then eluted from the silicon carbide. The kit comprises a buffer binding to be diluted with alcohol to provide an alcohol concentration of about 1 to about 50% (v/v), a wash solution, an elution solution, silicon carbide and instructions for adjusting the alcohol concentration to selectively bind nucleic acids having a size above the desired cut-off size.

    METHODS AND KITS FOR IMPROVING GLOBAL GENE EXPRESSION ANALYSIS OF HUMAN BLOOD, PLASMA AND/OR SERUM DERIVED RNA
    7.
    发明申请
    METHODS AND KITS FOR IMPROVING GLOBAL GENE EXPRESSION ANALYSIS OF HUMAN BLOOD, PLASMA AND/OR SERUM DERIVED RNA 审中-公开

    公开(公告)号：US20170268040A1

    公开(公告)日：2017-09-21

    申请号：US15460826

    申请日：2017-03-16

    申请人： Norgen Biotek Corp.

    发明人： Yousef Haj-Ahmad

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6806 ,  C12N15/1006 ,  C12Q1/6874 ,  C12Q2527/125 ,  C12Q2535/122 ,  C12Q2537/159 ,  C12Q2565/519

    摘要： Disclosed are methods and kits for improving global gene expression analysis for a population of RNA molecules derived from a human blood, plasma and/or serum sample. In an embodiment, the method comprises the step of selectively depleting 5′-RNAY4 fragments from the population of RNA molecules or selectively blocking 5′-RNAY4 fragments within the RNA population. The 5′-RNAY4 depleted or 5′-RNAY4 blocked population of RNA can be used in a variety of global gene expression analysis protocols, including next generation sequencing. In a further embodiment, the method comprises selectively depleting or blocking miR-486-5p fragments within the RNA population. The miR-486-5p depleted or miR-486-5p blocked population of RNA can also be used in global gene expression analysis protocols, including next generation sequencing. The kit comprises oligonucleotide probes comprising a nucleotide sequence that is the complement to a nucleotide sequence of the 5′ end of the RNAY4 and/or oligonucleotide probes comprising a nucleotide sequence that is the complement to a nucleotide sequence of miR-486-5p.

    METHODS AND KITS FOR IMPROVING GLOBAL GENE EXPRESSION ANALYSIS OF HUMAN URINE DERIVED RNA
    10.
    发明申请
    METHODS AND KITS FOR IMPROVING GLOBAL GENE EXPRESSION ANALYSIS OF HUMAN URINE DERIVED RNA 有权

    公开(公告)号：US20220090169A1

    公开(公告)日：2022-03-24

    申请号：US17545357

    申请日：2021-12-08

    申请人： Norgen Biotek Corp.

    发明人： Yousef Haj-Ahmad

    IPC分类号： C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6883

    摘要： Disclosed are methods and kits for improving global gene expression analysis for a population of RNA molecules derived from a human urine sample. In an embodiment, the method comprises the step of selectively depleting miR-10a-5p fragments from the population of RNA molecules or selectively blocking miR-10a-5p fragments within the RNA population. The miR-10a-5p depleted or miR-10a-5p blocked population of RNA can be used in a variety of global gene expression analysis protocols, including next generation sequencing. In a further embodiment, the method comprises selectively depleting or blocking miR-10b-5p fragments within the RNA population. The miR-10a-5p and/or miR-10b-5p depleted or blocked populations of RNA can also be used in global gene expression analysis protocols, including next generation sequencing. The kit comprises oligonucleotide probes comprising a nucleotide sequence that is the complement to a nucleotide sequence of the miR-10a-5p and/or oligonucleotide probes comprising a nucleotide sequence that is the complement to a nucleotide sequence of miR-10b-5p.