公开(公告)号：US07822558B2

公开(公告)日：2010-10-26

申请号：US11816244

申请日：2006-02-15

申请人： Noriaki Kimura ,  Kyoji Doi ,  Takayoshi Yumii ,  Takashi Yoshida ,  Shigeyuki Nakada ,  Hironori Hayashi ,  Kazuteru Hoshishima

发明人： Noriaki Kimura ,  Kyoji Doi ,  Takayoshi Yumii ,  Takashi Yoshida ,  Shigeyuki Nakada ,  Hironori Hayashi ,  Kazuteru Hoshishima

IPC分类号： G06F19/00

CPC分类号： G01N21/274 ,  G01N15/1459 ,  G01N21/6408 ,  G01N21/6428 ,  G01N21/645 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2021/6482

摘要： A fluorescence detecting device irradiates an object to be measured with a laser beam, receives fluorescence generated from the object and processes a fluorescence signal generated when receiving the fluorescence. The device includes a laser light source section outputting the laser beam for irradiating the object, a light receiving section outputting the fluorescence signal of the fluorescence generated by the irradiated object, a light source control section generates a modulation signal having a frequency in order to time-modulate an intensity of the laser beam, and a processing section that calculates a fluorescence relaxation time of the fluorescence of the object based on the fluorescence signal output from the light receiving section. From the detection values acquired by the device including the phase information on the fluorescence, the intensity of the fluorescence is calculated.

摘要翻译： 荧光检测装置用激光束照射待测物体，接收从物体产生的荧光，并处理在接收荧光时产生的荧光信号。 该装置包括输出用于照射物体的激光束的激光光源部分，输出被照射物体产生的荧光的荧光信号的光接收部分，光源控制部分产生具有频率的调制信号以便时间 - 调制激光束的强度，以及处理部，其基于从光接收部输出的荧光信号，计算对象的荧光的荧光弛豫时间。 根据由包括荧光的相位信息的装置获取的检测值，计算荧光强度。

公开(公告)号：US20090012721A1

公开(公告)日：2009-01-08

申请号：US11816244

申请日：2006-02-15

申请人： Noriaki Kimura ,  Kyoji Doi ,  Takayoshi Yumii ,  Takashi Yoshida ,  Shigeyuki Nakada ,  Hironori Hayashi ,  Kazuteru Hoshishima

发明人： Noriaki Kimura ,  Kyoji Doi ,  Takayoshi Yumii ,  Takashi Yoshida ,  Shigeyuki Nakada ,  Hironori Hayashi ,  Kazuteru Hoshishima

IPC分类号： G01N21/64 ,  G06F19/00

CPC分类号： G01N21/274 ,  G01N15/1459 ,  G01N21/6408 ,  G01N21/6428 ,  G01N21/645 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2021/6482

摘要： A fluroescene detecting device irradiates an object to be measured with a laser beam, receives fluroescene generated from the object and processes a fluorescence signal generated when receiving the fluroescene. The device includes a laser light source section outputting the laser beam for irradiating the object, a light receiving section outputting the fluorescene signal of the fluorescene generated by the irradiated object, a light source control section generates a modulatioon signal having a frequency in order to time-modulate an intensity of the laser beam, and a processing section that calculates a flurescene relaxation time of the fluorescene of the object based on the fluorescene relaxation time of the fluorescene of the object based on the fluorescene relaxation time of the fluorescene of the object based on the fluorescene signal output from the light receiving section. From the detection values acquired by the device including the phase information on the fluorescene, the intensity of the fluorescene is calculated.

摘要翻译： 氟草醚检测装置用激光束照射待测物体，接收从物体产生的氟草醚，并处理接收氟草醚时产生的荧光信号。 该装置包括输出用于照射物体的激光束的激光光源部分，输出由照射物体产生的荧光的荧光信号的光接收部分，光源控制部分产生具有频率的模数信号以便时间 - 调制激光束的强度，以及处理部，其基于物体的荧光的荧光弛豫时间，基于物体的荧光的荧光弛豫时间，计算物体的荧光的荧光弛豫时间 在从光接收部分输出的荧光信号上。 从包括荧光相位信息的装置获取的检测值，计算出荧光的强度。

公开(公告)号：US06984815B2

公开(公告)日：2006-01-10

申请号：US10489422

申请日：2002-09-26

申请人： Takayoshi Yumii ,  Kyoji Doi ,  Noriaki Kimura

发明人： Takayoshi Yumii ,  Kyoji Doi ,  Noriaki Kimura

IPC分类号： H01J40/14

CPC分类号： H04N5/32 ,  H04N5/374

摘要： An electronic pulse detection device includes: an MCP in which a plurality of capillaries configured to increase the number of electrons are arranged in matrix; and an electronic pulse reading chip 30 disposed on an output side of the MCP. The electronic pulse detection chip 30 includes anodes 32 and detection transistors 34 both provided so as to correspond to the respective capillaries. Electronic pulses are incident on the anodes 32 from the MCP. Drains of the detection transistors 34 are connected to the corresponding anodes. The detection transistors 34 on the same row are connected to one another at gates thereof and turned on/off as a unit, and sources of the detection transistors 34 on the same column are connected to a corresponding switch circuit 80 as a unit to be connected to a current-voltage conversion resistance RL via the switch circuit 80. The current-voltage conversion resistance RL converts a current, which flows therethrough when the electronic pulses are incident on the anodes 32, to a voltage and inputs the voltage to an operational amplifier 90. This makes it possible to detect feeble light and radiation ray two dimensionally.

摘要翻译： 电子脉冲检测装置包括：MCP，其中配置成增加电子数量的多个毛细管布置成矩阵; 以及设置在MCP的输出侧的电子脉冲读取芯片30。 电子脉冲检测芯片30包括阳极32和检测晶体管34，两者均设置成对应于相应的毛细管。 电子脉冲从MCP入射到阳极32上。 检测晶体管34的漏极连接到相应的阳极。 同一行的检测晶体管34在其栅极处彼此连接并作为单元导通/截止，并且同一列上的检测晶体管34的源极连接到相应的开关电路80作为要连接的单元 经由开关电路80到达电流 - 电压转换电阻R L L。 电流 - 电压转换电阻R L将在电子脉冲入射到阳极32上时流经的电流转换成电压并将电压输入到运算放大器90。 这使得可以二维地检测微弱的光和辐射线。

公开(公告)号：US20130220009A1

公开(公告)日：2013-08-29

申请号：US13883961

申请日：2011-11-04

申请人： Kazuteru Hoshishima ,  Noriaki Kimura

发明人： Kazuteru Hoshishima ,  Noriaki Kimura

IPC分类号： G01P13/00

CPC分类号： G01P13/0093 ,  G01M9/067 ,  G01P5/001 ,  G01S17/89

摘要： When a flow of a liquid body around a measuring object is visualized, a first liquid body as a tracer is supplied from a nozzle hole into a flow field of a second liquid body, and a laser beam having a wavelength optically absorbed by the first liquid body is irradiated in a manner such that the laser beam traverse across the flow field. At this point, the irradiation position of the laser beam is controlled in such a manner that the flow field is scanned with the laser beam. On the other hand, the laser beam that has passed through the flow field is received and a position where the first liquid body traverses the laser beam is obtained using the scan intensity signal of the received laser beam so that the flow of the second liquid body is visualized. The position where the first liquid body traverses the laser beam can be obtained based on a position on a time axis where a value of the scan intensity signal is less than a set threshold. According to the above described visualization, the flow field can reliably be visualized even for the high-speed fluid body.

摘要翻译： 当可视化测量物体周围的液体的流动时，将作为示踪剂的第一液体体从喷嘴孔供应到第二液体的流场中，并且具有由第一液体光学吸收的波长的激光束 以使得激光束横过流场的方式照射身体。 此时，以激光束扫描流场的方式控制激光束的照射位置。 另一方面，接收通过流场的激光束，利用所接收的激光束的扫描强度信号获得第一液体横穿激光束的位置，使第二液体的流动 是可视化的。 可以基于扫描强度信号的值小于设定阈值的时间轴上的位置来获得第一液体穿过激光束的位置。 根据上述可视化，即使对于高速流体体也可以可视地显示流场。

公开(公告)号：US09182422B2

公开(公告)日：2015-11-10

申请号：US13883961

申请日：2011-11-04

申请人： Kazuteru Hoshishima ,  Noriaki Kimura

发明人： Kazuteru Hoshishima ,  Noriaki Kimura

IPC分类号： G01N21/00 ,  G01P13/00 ,  G01M9/06 ,  G01P5/00 ,  G01S17/89

CPC分类号： G01P13/0093 ,  G01M9/067 ,  G01P5/001 ,  G01S17/89

摘要： When a flow of a liquid body around a measuring object is visualized, a first liquid body as a tracer is supplied from a nozzle hole into a flow field of a second liquid body, and a laser beam having a wavelength optically absorbed by the first liquid body is irradiated in a manner such that the laser beam traverse across the flow field. At this point, the irradiation position of the laser beam is controlled in such a manner that the flow field is scanned with the laser beam. On the other hand, the laser beam that has passed through the flow field is received and a position where the first liquid body traverses the laser beam is obtained using the scan intensity signal of the received laser beam so that the flow of the second liquid body is visualized. The position where the first liquid body traverses the laser beam can be obtained based on a position on a time axis where a value of the scan intensity signal is less than a set threshold. According to the above described visualization, the flow field can reliably be visualized even for the high-speed fluid body.

摘要翻译： 当可视化测量物体周围的液体的流动时，将作为示踪剂的第一液体体从喷嘴孔供应到第二液体的流场中，并且具有由第一液体光学吸收的波长的激光束 以使得激光束横过流场的方式照射身体。 此时，以激光束扫描流场的方式控制激光束的照射位置。 另一方面，接收通过流场的激光束，利用所接收的激光束的扫描强度信号获得第一液体横穿激光束的位置，使第二液体的流动 是可视化的。 可以基于扫描强度信号的值小于设定阈值的时间轴上的位置来获得第一液体穿过激光束的位置。 根据上述可视化，即使对于高速流体体也可以可视地显示流场。

公开(公告)号：US07326900B2

公开(公告)日：2008-02-05

申请号：US10533469

申请日：2002-11-18

申请人： Noriaki Kimura ,  Takayoshi Yumii

发明人： Noriaki Kimura ,  Takayoshi Yumii

IPC分类号： H01J40/14

CPC分类号： G01N21/6456 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2021/6463 ,  G01N2021/6473 ,  H01J31/507 ,  H01J43/045 ,  H01J43/243

摘要： A weak light detector (40) which can detect two-dimensional weak radiation at a high speed with high precision. The fluorescence from the DNA chip (46) is incident on a detection part (56) of a detection unit (52). The detection unit (56) has a detection module with a number of detection transistors being placed to correspond to cells of the DNA chip (46). The detection part (56) performs photoelectric conversion of the incident fluorescence (photon) to emit electrons, and amplifies the electrons to make them incident on the detection module. The detection transistors are switched based the Hadamard matrix to operate. A data processing unit (54) reads an output signal of the detection part (56), then performs Hadamard inversion, and determines the detection transistor which outputs the signal.

摘要翻译： 弱光检测器（40）可高精度检测二维弱辐射。 来自DNA芯片（46）的荧光入射到检测单元（52）的检测部分（56）上。 检测单元（56）具有检测模块，多个检测晶体管被放置成对应于DNA芯片（46）的单元。 检测部（56）进行入射荧光（光子）的光电转换，发射电子，放大电子，使其入射到检测模块上。 检测晶体管基于Hadamard矩阵进行切换操作。 数据处理单元（54）读取检测部分（56）的输出信号，然后执行哈达玛反转，并确定输出该信号的检测晶体管。

公开(公告)号：US20050072898A1

公开(公告)日：2005-04-07

申请号：US10489422

申请日：2002-09-26

申请人： Takayoshi Yumi ,  Kyoji Doi ,  Noriaki Kimura

发明人： Takayoshi Yumi ,  Kyoji Doi ,  Noriaki Kimura

IPC分类号： G01N21/64 ,  G01J1/02 ,  G01N33/53 ,  G01N33/566 ,  G01N37/00 ,  G01T1/20 ,  H01L27/14 ,  H01L27/144 ,  H01L27/146 ,  H04N3/15 ,  H04N5/32 ,  G01J1/00

CPC分类号： H04N5/32 ,  H04N5/374

摘要： An electronic pulse detection device includes: an MCP in which a plurality of capillaries configured to increase the number of electrons are arranged in matrix; and an electronic pulse reading chip 30 disposed on an output side of the MCP. The electronic pulse detection chip 30 includes anodes 32 and detection transistors 34 both provided so as to correspond to the respective capillaries. Electronic pulses are incident on the anodes 32 from the MCP. Drains of the detection transistors 34 are connected to the corresponding anodes. The detection transistors 34 on the same row are connected to one another at gates thereof and turned on/off as a unit, and sources of the detection transistors 34 on the same column are connected to a corresponding switch circuit 80 as a unit to be connected to a current-voltage conversion resistance RL via the switch circuit 80. The current-voltage conversion resistance RL converts a current, which flows therethrough when the electronic pulses are incident on the anodes 32, to a voltage and inputs the voltage to an operational amplifier 90. This makes it possible to detect feeble light and radiation ray two dimensionally.

摘要翻译： 电子脉冲检测装置包括：MCP，其中配置成增加电子数量的多个毛细管布置成矩阵; 以及设置在MCP的输出侧的电子脉冲读取芯片30。 电子脉冲检测芯片30包括阳极32和检测晶体管34，两者均设置成对应于相应的毛细管。 电子脉冲从MCP入射到阳极32上。 检测晶体管34的漏极连接到相应的阳极。 同一行的检测晶体管34在其栅极处彼此连接并作为单元导通/截止，并且同一列上的检测晶体管34的源极连接到相应的开关电路80作为要连接的单元 经由开关电路80连接到电流 - 电压转换电阻RL。电流 - 电压转换电阻RL将当电子脉冲入射到阳极32上时流过的电流转换成电压并将电压输入到运算放大器 这使得可以二维地检测微弱的光和辐射线。

公开(公告)号：US20100312482A1

公开(公告)日：2010-12-09

申请号：US12675323

申请日：2007-08-30

申请人： Shigeyuki Nakada ,  Noriaki Kimura

发明人： Shigeyuki Nakada ,  Noriaki Kimura

IPC分类号： G06F19/00 ,  G01N21/64 ,  G01J1/58

CPC分类号： G01N21/6428 ,  G01N21/6408 ,  G01N2021/6441

摘要： When FRET efficiency is measured quantitatively by removing uncertain elements of fluorescence detection information, calibration information prestored in a storage means while including at least the leak rate of donor fluorescence component emitted from a donor molecule, the leak rate of acceptor fluorescence component emitted from an acceptor molecule, and the non-FRET fluorescence lifetime of the donor fluorescence component when FRET is not generated out of the fluorescence of a measurement object sample is acquired. The FRET fluorescence lifetime of the donor fluorescence component is then determined using the intensity information and phase information of fluorescence of the measurement object sample, the leak rate of donor fluorescence component and the leak rate of acceptor fluorescence component, thus determining the FRET fluorescence efficiency.

摘要翻译： 当通过消除荧光检测信息的不确定因素，预先存储在存储装置中的校准信息同时至少包括从供体分子发射的供体荧光成分的泄漏率，从受体发射的受体荧光成分的泄漏率，定量测量FRET效率 并且获得当测量对象样品的荧光不产生FRET时供体荧光成分的非FRET荧光寿命。 然后使用测量对象样品的荧光强度信息和相位信息，供体荧光成分的泄漏率和受体荧光成分的泄漏率来确定供体荧光成分的FRET荧光寿命，从而确定FRET荧光效率。

公开(公告)号：US08614099B2

公开(公告)日：2013-12-24

申请号：US12675323

申请日：2007-08-30

申请人： Shigeyuki Nakada ,  Noriaki Kimura

发明人： Shigeyuki Nakada ,  Noriaki Kimura

IPC分类号： G01N21/76 ,  G01N21/64

CPC分类号： G01N21/6428 ,  G01N21/6408 ,  G01N2021/6441

摘要： When FRET efficiency is measured quantitatively by removing uncertain elements of fluorescence detection information, calibration information prestored in a storage means while including at least the leak rate of donor fluorescence component emitted from a donor molecule, the leak rate of acceptor fluorescence component emitted from an acceptor molecule, and the non-FRET fluorescence lifetime of the donor fluorescence component when FRET is not generated out of the fluorescence of a measurement object sample is acquired. The FRET fluorescence lifetime of the donor fluorescence component is then determined using the intensity information and phase information of fluorescence of the measurement object sample, the leak rate of donor fluorescence component and the leak rate of acceptor fluorescence component, thus determining the FRET fluorescence efficiency.

摘要翻译： 当通过消除荧光检测信息的不确定因素，预先存储在存储装置中的校准信息同时至少包括从供体分子发射的供体荧光成分的泄漏率，从受体发射的受体荧光成分的泄漏率，定量测量FRET效率 并且获得当测量对象样品的荧光不产生FRET时供体荧光成分的非FRET荧光寿命。 然后使用测量对象样品的荧光强度信息和相位信息，供体荧光成分的泄漏率和受体荧光成分的泄漏率来确定供体荧光成分的FRET荧光寿命，从而确定FRET荧光效率。

公开(公告)号：US08642306B2

公开(公告)日：2014-02-04

申请号：US12663373

申请日：2008-06-11

申请人： Shigeyuki Nakada ,  Noriaki Kimura ,  Akihide Ito ,  Kenji Yasuda

发明人： Shigeyuki Nakada ,  Noriaki Kimura ,  Akihide Ito ,  Kenji Yasuda

IPC分类号： C12N13/00

CPC分类号： G01N15/147 ,  C12M47/04 ,  G01N2015/149

摘要： A liquid sample flow containing living cells is irradiated with measurement laser light and the photo data of at least either scattering light or fluorescence that is generated by each of the living cells in the liquid sample flow due to the irradiation with the measurement laser light is acquired. Based on the photo data thus acquired, it is determined whether each of the cells assignable to the respective photo data is an unnecessary living cell or a target living cell. Based on the determination results, a pulse voltage is then applied exclusively to the living cells having been determined as unnecessary living cells so that the unnecessary living cells are damaged and killed.

摘要翻译： 用测量激光照射含有活细胞的液体样品流，并且获取由于测量激光的照射而在液体样品流中的每个活细胞产生的至少一种散射光或荧光的照片数据 。 基于这样获得的照片数据，确定分配给各个照片数据的每个细胞是否是不必要的活细胞或目标活细胞。 基于测定结果，脉冲电压被专门用于被确定为不必要的活细胞的活细胞，使得不必要的活细胞被损坏和死亡。