公开(公告)号：US20050130144A1

公开(公告)日：2005-06-16

申请号：US10490302

申请日：2002-08-30

申请人： Norio Nakatsuji ,  Masako Tada ,  Takashi Tada

发明人： Norio Nakatsuji ,  Masako Tada ,  Takashi Tada

IPC分类号： A01K67/027 ,  A61L27/38 ,  C12N5/16 ,  G01N33/50 ,  C12Q1/68 ,  C12N5/08 ,  C12N15/85

CPC分类号： G01N33/5088 ,  A01K67/027 ,  A01K67/0271 ,  A61L27/36 ,  A61L27/3895 ,  C12N5/16 ,  C12N2517/10 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5044 ,  G01N33/5073 ,  G01N2500/00

摘要： By exposing somatic cells to a component derived from ES cells to act on somatic cells and detecting the activity thereof, the reprogramming agent can be screened. Further, by exposing somatic cells to a component including a reprogramming agent or an isolated reprogramming agent, the somatic cells can be reprogrammed. Furthermore, according to the present invention, since the tetraploid cells have proliferating capability and pluripotency, such cells can be differentiated and the cells, tissues or organs, which can be used for transplantation, can be produced.

摘要翻译： 通过将体细胞暴露于源自ES细胞的成分作用于体细胞并检测其活性，可以筛选重编程剂。 此外，通过将体细胞暴露于包括重编程剂或分离的重编程剂的组分，体细胞可被重新编程。 此外，根据本发明，由于四倍体细胞具有增殖能力和多能性，因此可以分化这些细胞，并且可以生产可用于移植的细胞，组织或器官。

公开(公告)号：US20050084968A1

公开(公告)日：2005-04-21

申请号：US10490177

申请日：2002-09-20

申请人： Norio Nakatsuji ,  Takashi Tada ,  Masako Tada

发明人： Norio Nakatsuji ,  Takashi Tada ,  Masako Tada

IPC分类号： C12N15/02 ,  A61L27/00 ,  A61L27/38 ,  C12N5/0735 ,  C12N5/10 ,  C12N5/16 ,  C12N5/08 ,  C12N15/85

CPC分类号： A61L27/3895 ,  A61K2035/122 ,  A61L27/3604 ,  A61L27/3834 ,  C12N5/0606 ,  C12N5/16 ,  C12N2510/00 ,  C12N2510/02

摘要： An object of the present invention is to efficiently establish cells, tissues, and organs capable of serving as donors for treating diseases, without eliciting immune rejection reactions, without starting with an egg cell. This object was achieved by providing a pluripotent stem cell having a desired genome. The cell was produced by treating with a reprogramming agent, producing a fusion cell of an MHC deficient stem cell with a somatic cell, or after producing a fusion cell of a stem cell with a somatic cell, removing a gene derived from the stem cell by performing genetic manipulation with a retrovirus.

摘要翻译： 本发明的一个目的是有效地建立能够作为用于治疗疾病的供体的细胞，组织和器官，而不引发免疫排斥反应，而不是从卵细胞开始。 该目的通过提供具有所需基因组的多能干细胞来实现。 通过用编程剂处理细胞，产生具有体细胞的MHC缺陷型干细胞的融合细胞，或在产生具有体细胞的干细胞的融合细胞后，通过以下步骤除去源自干细胞的基因： 用逆转录病毒进行遗传操作。

公开(公告)号：US20080003560A1

公开(公告)日：2008-01-03

申请号：US11818935

申请日：2007-06-14

申请人： Norio Nakatsuji ,  Takashi Tada ,  Masako Tada

发明人： Norio Nakatsuji ,  Takashi Tada ,  Masako Tada

IPC分类号： C12N15/07 ,  C12N5/06

CPC分类号： A61L27/3895 ,  A61K2035/122 ,  A61L27/3604 ,  A61L27/3834 ,  C12N5/0606 ,  C12N5/16 ,  C12N2510/00 ,  C12N2510/02

摘要： An object of the present invention is to efficiently establish cells, tissues, and organs capable of serving as donors for treating diseases, without eliciting immune rejection reactions, without starting with an egg cell. This object was achieved by providing a pluripotent stem cell having a desired genome. The cell was produced by treating with a reprogramming agent, producing a fusion cell of an MHC deficient stem cell with a somatic cell, or after producing a fusion cell of a stem cell with a somatic cell, removing a gene derived from the stem cell by performing genetic manipulation with a retrovirus.

摘要翻译： 本发明的一个目的是有效地建立能够作为用于治疗疾病的供体的细胞，组织和器官，而不引发免疫排斥反应，而不是从卵细胞开始。 该目的通过提供具有所需基因组的多能干细胞来实现。 通过用编程剂处理细胞，产生具有体细胞的MHC缺陷型干细胞的融合细胞，或在产生具有体细胞的干细胞的融合细胞后，通过以下步骤除去源自干细胞的基因： 用逆转录病毒进行遗传操作。

公开(公告)号：US20060288431A1

公开(公告)日：2006-12-21

申请号：US10545368

申请日：2004-02-12

申请人： Norio Nakatsuji ,  Takashi Tada ,  Masako Tada

发明人： Norio Nakatsuji ,  Takashi Tada ,  Masako Tada

IPC分类号： A01K67/027 ,  C12Q1/68 ,  C07H21/04 ,  C12P21/06 ,  C07K14/705 ,  C07K16/28

CPC分类号： C07K14/47 ,  C12N5/0607 ,  C12N2510/00

摘要： A gene is provided, which can be used as a marker for determining whether a certain cell, particularly an undifferentiated cell including a tissue stem cell, has pluripotency or an undifferentiated state. The gene is called Stm and includes a Stm1 gene, which is expressed specifically in a cell under an undifferentiated state if the cell has pluripotency. A kit for determining a differentiated state of a cell is also provided. The kit comprises (a) an agent capable of reacting specifically with a Stm gene or a Stm gene product; and (b) means for determining whether or not the Stm gene is expressed in the cell.

摘要翻译： 提供了可用作确定特定细胞，特别是包括组织干细胞的未分化细胞是否具有多能性或未分化状态的标记的基因。 该基因称为Stm，包括Stm1基因，如果细胞具有多能性，其在未分化状态的细胞中特异性表达。 还提供了用于确定细胞分化状态的试剂盒。 试剂盒包含（a）能够与Stm基因或Stm基因产物特异性反应的试剂; 和（b）用于确定Stm基因是否在细胞中表达的手段。

公开(公告)号：US20090264312A1

公开(公告)日：2009-10-22

申请号：US11813930

申请日：2006-01-12

申请人： Takashi Tada ,  Norio Nakatsuji ,  Hiroyuki Matsumura ,  Masako Tada

发明人： Takashi Tada ,  Norio Nakatsuji ,  Hiroyuki Matsumura ,  Masako Tada

IPC分类号： C40B40/02 ,  C12N15/74 ,  C12N15/00 ,  C12N5/10

CPC分类号： A61K35/48 ,  C12N5/0081 ,  C12N5/12 ,  C12N15/907 ,  C12N2510/00 ,  C12N2800/30 ,  C12N2840/203

摘要： It is intended to provide a regenerable cell in which a desired chromosome has been deleted, a method for producing the cell, and a gene cassette and a kit to be used for the method. More particularly, it is intended to obtain an individual corresponding pluripotent stem cell easily and simply. More specifically, it is intended to efficiently establish a cell, tissue or organ that can be a donor for treating a disease without causing immune rejection response, without newly obtaining and establishing a stem cell such as ES cell from the individual, and without using an ovum as a material. It was achieved by a gene cassette in which not two recombinase recognition sites in a cis orientation are inserted, but one recombinase recognition site or two recombinase recognition sites in an opposite orientation is/are inserted and a marker gene is connected, and by application of the gene cassette to a cell fusion technique.

摘要翻译： 旨在提供其中缺失所需染色体的可再生细胞，生产细胞的方法，以及用于该方法的基因盒和试剂盒。 更具体地，旨在容易且简单地获得单个相应的多能干细胞。 更具体地，旨在有效地建立可以是用于治疗疾病而不引起免疫排斥反应的供体的细胞，组织或器官，而不从个体中新获得和建立诸如ES细胞的干细胞，并且不使用 卵子为材料。 通过插入不具有顺式取向的两个重组酶识别位点的基因盒来实现，但是插入一个相反方向的一个重组酶识别位点或两个重组酶识别位点，并连接标记基因，并通过应用 基因盒到细胞融合技术。

公开(公告)号：US07083977B2

公开(公告)日：2006-08-01

申请号：US10311146

申请日：2001-06-15

申请人： Norio Nakatsuji ,  Takashi Tada ,  Ryuzo Torii ,  Yoshihiko Hosoi ,  Akira Iritani ,  Teruo Akuta

发明人： Norio Nakatsuji ,  Takashi Tada ,  Ryuzo Torii ,  Yoshihiko Hosoi ,  Akira Iritani ,  Teruo Akuta

IPC分类号： C12Q1/68 ,  C12N5/00 ,  C12N5/06

CPC分类号： C12N5/0606 ,  C12N2503/00 ,  C12N2503/02

摘要： A method for producing a monkey-derived embryonic stem cell comprising the steps of carrying out fertilization by insemination by in vitro fertilization or intracytoplasmic sperm injection using a monkey ovum and monkey sperms, thereby giving a fertilized ovum, allowing the fertilized ovum to differentiate into a blastocyst by in vitro culture, and establishing an ES cell line using the blastocyst; the monkey ES cell obtained by the method, a method for screening a reagent for specific differentiation into cell or tissue by using the ES cell; and a differentiated cell or differentiated tissue each differentiated from the ES cell. According to the present invention, applications of the embryonic stem cells to embryological studies clinical applications, experimental models, and the like on primates, studies of diseases, are expected.

公开(公告)号：US08546014B2

公开(公告)日：2013-10-01

申请号：US12981003

申请日：2010-12-29

申请人： Takashi Tada

发明人： Takashi Tada

IPC分类号： H01M2/04 ,  H01M2/02

CPC分类号： H01M2/1066

摘要： A battery chamber includes: a claw mechanism for a battery including: a movable member that is movable in insertion and removal directions of a battery, and abuts a front face of the battery in the insertion direction when the battery is inserted and completely loaded; an urging member that urges the movable member in the removal direction; and a stopper having: a locking claw that locks a back face of the battery from behind in the insertion direction when the battery is completely loaded, wherein in a case where the battery is inserted, and the movable member is pressed and moved in the insertion direction by the battery, and then an interval in the insertion direction between the movable member and the locking claw becomes a length of the battery in the insertion direction, the locking claw moves from an evacuation position of the locking claw where the locking claw does not interfere with the battery when the battery is inserted to a locking position of the locking claw where the locking claw locks the back face of the battery from behind in the insertion direction when the battery is completely loaded.

摘要翻译： 电池室包括：用于电池的爪机构，包括：可动构件，其可在电池的插入和取出方向上移动，并且当电池插入并完全装载时沿插入方向抵靠电池的正面; 推动构件，其沿移除方向推动可动构件; 以及止动器，其具有：当所述电池被完全装载时，在所述插入方向上从所述插入方向的后方锁定所述电池的背面的锁定爪，其中，在插入所述电池的情况下，并且所述可动构件在所述插入中被按压和移动 然后在可动构件和锁定爪之间的插入方向上的间隔成为电池在插入方向上的长度，锁定爪从锁定爪不在锁定爪的抽空位置移动 当电池被插入锁定爪的锁定位置时，当电池被插入锁定爪的锁定位置时，当电池被完全装载时，锁定爪在插入方向上从后面锁定电池的背面。

公开(公告)号：US20110216237A1

公开(公告)日：2011-09-08

申请号：US13128511

申请日：2009-11-26

申请人： Junichi Shinohara ,  Atsushi Sato ,  Ryota Kaga ,  Takashi Tada ,  Shu Kambe ,  Norihiro Yoshida

发明人： Junichi Shinohara ,  Atsushi Sato ,  Ryota Kaga ,  Takashi Tada ,  Shu Kambe ,  Norihiro Yoshida

IPC分类号： H04N5/225

CPC分类号： G03B17/02 ,  H04N5/2252 ,  H04N5/23209

摘要： A camera body (1) of the present invention is provided with a concave portion (1A) in which an imaging unit (2) including a rectangular solid shaped case (2a) having an optical system and an imaging device is detachably disposed. When the case (2a) is viewed from a front side in a state where the case (2a) is disposed in the concave portion (1A), the camera body (1) includes a back face portion (1B) having a back face wall (4) located on the back of the case (2a) and facing a back face of the case (2a), a side face portion (1C) having a side face wall (6) facing a side face of the case (2a), and an upper face portion (1D) having an upper face wall (5) facing an upper face of the case (2a). The concave portion (1A) is defined by the back face wall (4), the side face wall (6), and the upper face wall (5), and is open on three sides where the remaining faces of the case (2a) are located at the time of attachment.

摘要翻译： 本发明的照相机体（1）具有凹部（1A），在该凹部（1A）中，具有光学系统的矩形的实心形状的壳体（2a）和成像装置的成像单元（2）可拆卸地设置在该凹部中。 当在壳体（2a）设置在凹部（1A）中的状态下从前侧观察壳体（2a）时，照相机体（1）包括背面部（1B），背面部 （2a）的背面上的面对壳体（2a）的背面的侧面部（1C），具有面对壳体（2a）的侧面的侧面壁（6） 以及具有面向壳体（2a）的上表面的上表面壁（5）的上表面部分（1D）。 凹部（1A）由背面壁（4），侧面壁（6）和上表面壁（5）形成，并且在三个面上开口，其中壳体（2a）的其余面 位于附件时。

公开(公告)号：US10863066B2

公开(公告)日：2020-12-08

申请号：US16424513

申请日：2019-05-29

申请人： Takashi Tada

发明人： Takashi Tada

IPC分类号： H04N5/225 ,  H01Q1/22

摘要： An imaging device includes a casing; a plurality of optical systems held by the casing; and a communication antenna held by the casing. The communication antenna is disposed in an area outside an optical path of light of a maximum angle of view of each of the plurality of optical systems.

公开(公告)号：US08736753B2

公开(公告)日：2014-05-27

申请号：US13392959

申请日：2010-09-13

申请人： Ryota Kaga ,  Atsushi Sato ,  Junichi Shinohara ,  Shu Kambe ,  Takashi Tada

发明人： Ryota Kaga ,  Atsushi Sato ,  Junichi Shinohara ,  Shu Kambe ,  Takashi Tada

IPC分类号： H04N5/225 ,  G03B17/02

CPC分类号： H04N5/2252 ,  G03B13/02 ,  G03B17/14 ,  G03B17/56 ,  G03B2206/00

摘要： A camera body includes a recess to which an image pickup unit is detachably attached to be placed therein. The image pickup unit includes a housing having an optical system, an image pickup device, a first connector section. The recess has three wall sections including a back wall section facing a rear of the housing of the image pickup unit and a side wall section facing a side surface of the housing when the image pickup unit is placed in the recess. The side wall section includes a second connector section to be connected to the first connector section to perform communication. The back wall section is provided with a controlling member. A rear of the image pickup unit has an insertion opening into which the controlling member is to be inserted and an abutting section to allow the controlling member to abut thereon. When the image pickup unit is attached to the recess, the first connector section and the second connector section are connected to each other when the controlling member is inserted into the insertion opening, and the first connector section is not connected to the second connector section when the controlling member abuts on the abutting section.

摘要翻译： 相机主体包括凹部，图像拾取单元可拆卸地附接到凹部。 图像拾取单元包括具有光学系统的壳体，图像拾取装置，第一连接器部分。 凹部具有三个壁部分，其包括面向图像拾取单元的壳体的后部的后壁部分和当图像拾取单元放置在凹部中时面向壳体的侧表面的侧壁部分。 侧壁部分包括第二连接器部分，其连接到第一连接器部分以进行通信。 后壁部设置有控制构件。 图像拾取单元的后部具有插入开口，控制构件将插入该插入口中，并具有抵接部分，以允许控制构件抵靠在其上。 当图像拾取单元附接到凹部时，当控制构件插入到插入口中时，第一连接器部分和第二连接器部分彼此连接，并且第一连接器部分未连接到第二连接器部分，当 所述控制构件邻接所述抵接部。