公开(公告)号：US10767208B2

公开(公告)日：2020-09-08

申请号：US14342764

申请日：2012-09-06

申请人： Norman C. Nelson ,  Jijumon Chelliserry ,  Steven T. Brentano ,  Dmitry Lyakhov ,  Matthew C. Friedenberg ,  Anne-Laure Shapiro

发明人： Norman C. Nelson ,  Jijumon Chelliserry ,  Steven T. Brentano ,  Dmitry Lyakhov ,  Matthew C. Friedenberg ,  Anne-Laure Shapiro

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12Q1/6869 ,  C12Q1/6855

摘要： The invention provides compositions and methods for making closed nucleic acid structures in which one or both strands are continuous. The closed nucleic acid structures can be used as sequencing templates among other applications.

公开(公告)号：US20140329282A1

公开(公告)日：2014-11-06

申请号：US14342764

申请日：2012-09-06

申请人： Norman C. Nelson ,  Jijumon Chelliserry ,  Steven T. Brentano ,  Dmitry Lyakhov ,  Matthew C. Friedenberg ,  Anne-Laure Shapiro

发明人： Norman C. Nelson ,  Jijumon Chelliserry ,  Steven T. Brentano ,  Dmitry Lyakhov ,  Matthew C. Friedenberg ,  Anne-Laure Shapiro

IPC分类号： C12P19/34

摘要： The invention provides compositions and methods for making closed nucleic acid structures in which one or both strands are continuous. The closed nucleic acid structures can be used as sequencing templates among other applications.

摘要翻译： 本发明提供用于制备其中一条或两条链是连续的封闭核酸结构的组合物和方法。 封闭的核酸结构可以用作其他应用中的测序模板。

公开(公告)号：US08198027B2

公开(公告)日：2012-06-12

申请号：US11962072

申请日：2007-12-20

申请人： Steven T. Brentano ,  Dmitry Lyakhov ,  James D. Carlson ,  Norman C. Nelson ,  Lyle J. Arnold, Jr.

发明人： Steven T. Brentano ,  Dmitry Lyakhov ,  James D. Carlson ,  Norman C. Nelson ,  Lyle J. Arnold, Jr.

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

CPC分类号： C12Q1/6881 ,  C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6865 ,  C12Q2525/155 ,  C12Q2525/186 ,  C12Q2525/197 ,  C12Q2531/143 ,  C12Q2565/543

摘要： Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.

摘要翻译： 公开了用于体外核酸扩增的组合物，其包括目标特异性通用（TSU）启动子引物或启动子提供者寡核苷酸，其包括与扩增的靶序列特异性杂交的靶特异性（TS）序列和通用 （U）序列，通过使用通用序列的引物引入扩增的序列。 公开了体外核酸扩增方法，其中使用一种或多种TSU寡核苷酸将U序列连接到目标捕获步骤中的靶核酸，然后在基本上等温条件下进行的随后扩增步骤中使用U序列引物 制备含有指示样品中靶核酸存在的U序列的扩增产物。

公开(公告)号：US09169512B2

公开(公告)日：2015-10-27

申请号：US13380018

申请日：2010-07-01

申请人： Steven T. Brentano ,  Dmitry Lyakhov ,  James D. Carlson ,  Michael M. Becker ,  Norman C. Nelson ,  Lyle J. Arnold, Jr.

发明人： Steven T. Brentano ,  Dmitry Lyakhov ,  James D. Carlson ,  Michael M. Becker ,  Norman C. Nelson ,  Lyle J. Arnold, Jr.

IPC分类号： C12P19/34 ,  C12Q1/68

CPC分类号： C12Q1/6865 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q2549/119 ,  C12Q2537/143 ,  C12Q2531/143

摘要： Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.

摘要翻译： 用于进行扩增反应的组合物，反应混合物和方法，包括多重扩增反应，其中所述方法包括使用包含连接的第一和第二扩增寡聚体成员的一种或多种扩增寡聚体复合物。 一方面，将扩增寡聚物复合物与靶核酸杂交，然后捕获具有杂交扩增寡聚物复合物的靶核酸，并洗涤其它组分。 靶核酸的靶序列被预扩增以产生第一扩增产物。 第一扩增产物在一个或多个二级扩增反应中扩增以产生第二扩增产物。

公开(公告)号：US08642268B2

公开(公告)日：2014-02-04

申请号：US13460341

申请日：2012-04-30

申请人： Steven T. Brentano ,  Dmitry Lyakhov ,  James D. Carlson ,  Norman C. Nelson ,  Lyle J. Arnold, Jr. ,  Michael M. Becker

发明人： Steven T. Brentano ,  Dmitry Lyakhov ,  James D. Carlson ,  Norman C. Nelson ,  Lyle J. Arnold, Jr. ,  Michael M. Becker

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H19/00

CPC分类号： C12Q1/6881 ,  C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6865 ,  C12Q2525/155 ,  C12Q2525/186 ,  C12Q2525/197 ,  C12Q2531/143 ,  C12Q2565/543

摘要： Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.

摘要翻译： 公开了用于体外核酸扩增的组合物，其包括目标特异性通用（TSU）启动子引物或启动子提供者寡核苷酸，其包括与扩增的靶序列特异性杂交的靶特异性（TS）序列和通用 （U）序列，通过使用通用序列的引物引入扩增的序列。 公开了体外核酸扩增方法，其中使用一种或多种TSU寡核苷酸将U序列连接到目标捕获步骤中的靶核酸，然后在基本上等温条件下进行的随后扩增步骤中使用U序列引物 制备含有指示样品中靶核酸存在的U序列的扩增产物。

公开(公告)号：US08512955B2

公开(公告)日：2013-08-20

申请号：US12828676

申请日：2010-07-01

申请人： Steven T. Brentano ,  Dmitry Lyakhov ,  Norman C. Nelson ,  James D. Carlson ,  Michael M. Becker ,  Lyle J. Arnold, Jr.

发明人： Steven T. Brentano ,  Dmitry Lyakhov ,  Norman C. Nelson ,  James D. Carlson ,  Michael M. Becker ,  Lyle J. Arnold, Jr.

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

CPC分类号： C12Q1/6865 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q2549/119 ,  C12Q2537/143 ,  C12Q2531/143

摘要： Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.

公开(公告)号：US20120178636A1

公开(公告)日：2012-07-12

申请号：US13380018

申请日：2010-07-01

申请人： Steven T. Brentano ,  Dmitry Lyakhov ,  James D. Carlson ,  Michael M. Becker ,  Norman C. Nelson ,  Lyle J. Arnold, JR.

发明人： Steven T. Brentano ,  Dmitry Lyakhov ,  James D. Carlson ,  Michael M. Becker ,  Norman C. Nelson ,  Lyle J. Arnold, JR.

IPC分类号： C40B30/00

CPC分类号： C12Q1/6865 ,  C12Q1/6844 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q2549/119 ,  C12Q2537/143 ,  C12Q2531/143

摘要： Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.

摘要翻译： 用于进行扩增反应的组合物，反应混合物和方法，包括多重扩增反应，其中所述方法包括使用包含连接的第一和第二扩增寡聚体成员的一种或多种扩增寡聚体复合物。 一方面，将扩增寡聚物复合物与靶核酸杂交，然后捕获具有杂交扩增寡聚物复合物的靶核酸，并洗涤其它组分。 靶核酸的靶序列被预扩增以产生第一扩增产物。 第一扩增产物在一个或多个二级扩增反应中扩增以产生第二扩增产物。

公开(公告)号：US20140080728A1

公开(公告)日：2014-03-20

申请号：US14004107

申请日：2012-03-12

申请人： Norman C. Nelson ,  Jijumon Chelliserry

发明人： Norman C. Nelson ,  Jijumon Chelliserry

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6811 ,  C12N15/1065 ,  G06F19/20 ,  G06F19/22

摘要： Methods for selecting tag-oligonucleotide sequences for use in an in vitro nucleic acid assay. The selected tag sequences are useful for nucleic acid assay wherein interference between the nucleic acid sequences is the assay is to be controlled. Selected tag sequences are incorporated into nucleic acid assay to improve the performance of and/or minimize any interference between nucleic acids in the assay compared to untagged assays.

摘要翻译： 用于选择用于体外核酸测定的标签 - 寡核苷酸序列的方法。 所选择的标签序列可用于核酸测定，其中核酸序列之间的干扰是待测定的。 将选择的标签序列并入核酸测定中以改善与未标记测定相比在分析中核酸之间的任何干扰的性能和/或最小化。

公开(公告)号：US09512467B2

公开(公告)日：2016-12-06

申请号：US14004107

申请日：2012-03-12

申请人： Norman C. Nelson ,  Jijumon Chelliserry

发明人： Norman C. Nelson ,  Jijumon Chelliserry

IPC分类号： C12Q1/68 ,  G06F19/20 ,  C12N15/10

CPC分类号： C12Q1/6811 ,  C12N15/1065 ,  G06F19/20 ,  G06F19/22

摘要： Methods for selecting tag-oligonucleotide sequences for use in an in vitro nucleic acid assay. The selected tag sequences are useful for nucleic acid assay wherein interference between the nucleic acid sequences is the assay is to be controlled. Selected tag sequences are incorporated into nucleic acid assay to improve the performance of and/or minimize any interference between nucleic acids in the assay compared to untagged assays.

摘要翻译： 用于选择用于体外核酸测定的标签 - 寡核苷酸序列的方法。 所选择的标签序列可用于核酸测定，其中核酸序列之间的干扰是待测定的。 将选择的标签序列并入核酸测定中以改善与未标记测定相比在分析中核酸之间的任何干扰的性能和/或最小化。

公开(公告)号：US07696337B2

公开(公告)日：2010-04-13

申请号：US11574307

申请日：2005-08-26

申请人： Michael M. Becker ,  Steven T. Brentano ,  Daniel P. Kolk ,  Wai-Chung Lam ,  Kristin W. Livezey ,  Norman C. Nelson ,  Astrid R. W. Schroder ,  Gary P. Schroth

发明人： Michael M. Becker ,  Steven T. Brentano ,  Daniel P. Kolk ,  Wai-Chung Lam ,  Kristin W. Livezey ,  Norman C. Nelson ,  Astrid R. W. Schroder ,  Gary P. Schroth

IPC分类号： C07H21/04 ,  C12Q1/68

CPC分类号： C12P19/34 ,  C12Q1/6865 ,  C12Q2537/163 ,  C12Q2521/325 ,  C12Q2521/107 ,  C12Q2525/186 ,  C12Q2533/101 ,  C12Q2525/143

摘要： The present invention is directed to novel methods of synthesizing multiple copies of a target nucleic acid sequence which are autocatalytic (i.e., able to cycle automatically without the need to modify reaction conditions such as temperature, pH, or ionic strength and using the product of one cycle in the next one). In particular, the present invention discloses a method of nucleic acid amplification which is robust and efficient, while reducing the appearance of side products. The method uses only one primer, the “priming oligonucleotide,” a 3′blocked promoter oligonucleotide and optionally, a means for terminating a primer extension reaction, to amplify RNA or DNA molecules in vitro, while reducing or eliminating the formation of side products. The method of the present invention minimizes or eliminates the emergence of side products, thus providing a high level of specificity. Furthermore, the appearance of side products can complicate the analysis of the amplification reaction by various molecular detection techniques. The present invention minimizes or eliminates this problem, thus providing an enhanced level of sensitivity.

摘要翻译： 本发明涉及合成目标核酸序列的多个拷贝的新型方法，所述目标核酸序列是自动催化的（即，能够自动循环而不需要修饰诸如温度，pH或离子强度的反应条件，并且使用一种 循环在下一个）。 特别地，本发明公开了一种鲁棒有效的核酸扩增方法，同时减少了副产物的外观。 该方法仅使用一种引物，即引物寡核苷酸，3'封闭的启动子寡核苷酸和任选的终止引物延伸反应的手段，以体外扩增RNA或DNA分子，同时减少或消除副产物的形成。 本发明的方法使副产物的出现最小化或消除，从而提供高水平的特异性。 此外，副产物的出现可能通过各种分子检测技术使扩增反应的分析复杂化。 本发明使这个问题最小化或消除了这一点，从而提供了增强的灵敏度。