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公开(公告)号：US5503933A
公开(公告)日：1996-04-02
申请号：US201906
申请日：1994-02-25
申请人： Noubar B. Afeyan , Fred E. Regnier , Ning Mu
发明人： Noubar B. Afeyan , Fred E. Regnier , Ning Mu
CPC分类号： B01D15/08 , B01J20/261 , B01J20/264 , B01J20/265 , B01J20/267 , B01J20/28004 , B01J20/28078 , B01J20/286 , B01J20/3092 , B01J20/321 , B01J20/3217 , B01J20/327 , B01J20/3282 , B01J2220/54 , B01J2220/58 , Y10T428/2998
摘要： Disclosed are hydrophilic coatings covalently bound to hydrophobic surfaces as well as methods for their production. To form the coated surfaces, a compound is provided which comprises a hydrophobic domain including an unsaturated group and a hydrophilic domain. Also provided is a hydrophobic surface comprising unsaturated groups. Molecules of the compound are adsorbed onto the hydrophobic surface, and the unsaturated groups in the hydrophobic domains of molecules of the compound then are covalently crosslinked to the unsaturated groups on the hydrophobic surface by a free radical reaction. In one embodiment, hydrophilic coatings may be covalently attached to hydrophobic polymers such as divinylbenzene crosslinked polystyrene. Hydrophilic coatings covalently attached to hydrophobic surfaces are obtained which are stable, which may be readily derivatized, and which are useful in a wide range of chromatography applications.
摘要翻译： 公开了共价结合到疏水表面的亲水涂层以及它们的生产方法。 为了形成涂覆的表面，提供了包含疏水结构域的化合物，其包括不饱和基团和亲水结构域。 还提供了包含不饱和基团的疏水性表面。 化合物的分子被吸附到疏水性表面上，然后通过自由基反应使化合物分子的疏水区域中的不饱和基团与疏水性表面上的不饱和基团共价交联。 在一个实施方案中，亲水涂层可以共价连接到疏水性聚合物如二乙烯基苯交联的聚苯乙烯。 获得共价连接到疏水性表面的亲水性涂层，其是稳定的，其可以容易地衍生化，并且其可用于广泛的色谱应用。
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公开(公告)号：US5833861A
公开(公告)日：1998-11-10
申请号：US800786
申请日：1997-02-14
CPC分类号： B01J20/285 , B01D15/345 , B01J20/28092 , B01J20/28095 , B01J2220/54 , G01N2030/528
摘要： Disclosed are chromatography methods and matrix geometries which permit high resolution, high productivity separation of mixtures of solutes, particularly biological materials. The method involves passing fluids through specially designed chromatography matrices at high flow rates. The matrices define first and second interconnected sets of pores and a high surface area for solute interaction in fluid communication with the members of the second set of pores. The first and second sets of pores are embodied, for example, as the interstices among particles and throughpores within the particles. The pores are dimensioned such that, at achievable high fluid flow rates, convective flow occurs in both pore sets, and the convective flow rate exceeds the rate of solute diffusion in the second pore set. This approach couples convective and diffusive mass transport to and from the active surface and permits increases in fluid velocity without the normally expected bandspreading.
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公开(公告)号：US5228989A
公开(公告)日：1993-07-20
申请号：US988028
申请日：1992-12-09
CPC分类号： B01J20/285 , B01D15/345 , B01J20/28092 , B01J20/28095 , B01J2220/54 , G01N2030/528
摘要： Disclosed are chromatography methods and matrix geometries which permit high resolution, high productivity separation of mixtures of solutes, particularly biological materials. The method involves passing fluids through specially designed chromatography matrices at high flow rates. The matrices define first and second interconnected sets of pores and a high surface area for solute interaction in fluid communication with the members of the second set of pores. The first and second sets of pores are embodied, for example, as the interstices among particles and throughpores within the particles. The pores are dimensioned such that, at achievable high fluid flow rates, convective flow occurs in both pore sets, and the convective flow rate exceeds the rate of solute diffusion in the second pore set. This approach couples convective and diffusive mass transport to and from the active surface and permits increases in fluid velocity without the normally expected bandspreading.
摘要翻译： 公开了色谱方法和基质几何形状，其允许溶质，特别是生物材料的混合物的高分辨率，高生产率分离。 该方法包括以高流速将流体通过专门设计的色谱基质。 这些基质定义了第一和第二相互连接的孔组，以及用于与第二组孔的构件流体连通的溶质相互作用的高表面积。 第一和第二组孔体现为例如颗粒内的颗粒和透孔之间的间隙。 孔的尺寸使得在可实现的高流体流速下，在两个孔组中都发生对流，并且对流流速超过第二孔组中溶质扩散的速率。 这种方法将对流和扩散质量传输与活性表面相耦合，并允许流体速度的增加而没有通常预期的带扩展。
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公开(公告)号：US06627875B2
公开(公告)日：2003-09-30
申请号：US09840633
申请日：2001-04-23
IPC分类号： B01D5944
CPC分类号： H01J49/0077 , G01N30/72 , H01J49/4265 , H01J49/428
摘要： The methods of the invention exploit the mass-to-charge ratio dependent motion of ions in an electrodynamic ion traps in synergy with a charge quenching process. In an electrodynamic ion trap, each mass-to-charge ratio is characterized by a unique set of frequencies of motion. Accordingly, by tailoring the time-varying electrical potential of the ion trap the invention controls which ions are allowed or retained in the ion trap and which ions are subjected to charge quenching reactions. Control of ion retention and charge quenching is used to improve sample throughput, dynamic mass range and signal discrimination in the mass spectrometry of multiply charged ions.
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公开(公告)号：US5605623A
公开(公告)日：1997-02-25
申请号：US375910
申请日：1995-01-20
CPC分类号： B01J20/285 , B01D15/345 , B01J20/28092 , B01J20/28095 , B01J2220/54 , G01N2030/528
摘要： Disclosed are chromatography methods and matrix geometries which permit high resolution, high productivity separation of mixtures of solutes, particularly biological materials. The method involves passing fluids through specially designed chromatography matrices at high flow rates. The matrices define first and second interconnected sets of pores and a high surface area for solute interaction in fluid communication with the members of the second set of pores. The first and second sets of pores are embodied, for example, as the interstices among particles and throughpores within the particles. The pores are dimensioned such that, at achievable high fluid flow rates, convective flow occurs in both pore sets, and the convective flow rate exceeds the rate of solute diffusion in the second pore set. This approach couples convective and diffusive mass transport to and from the active surface and permits increases in fluid velocity without the normally expected bandspreading.
摘要翻译： 公开了色谱方法和基质几何形状，其允许溶质，特别是生物材料的混合物的高分辨率，高生产率分离。 该方法包括以高流速将流体通过专门设计的色谱基质。 这些基质定义了第一和第二相互连接的孔组，以及用于与第二组孔的构件流体连通的溶质相互作用的高表面积。 第一和第二组孔体现为例如颗粒内的颗粒和透孔之间的间隙。 孔的尺寸使得在可实现的高流体流速下，在两个孔组中都发生对流，并且对流流速超过第二孔组中溶质扩散的速率。 这种方法将对流和扩散质量传输与活性表面相耦合，并允许流体速度的增加而没有通常预期的带扩展。
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公开(公告)号：US5376249A
公开(公告)日：1994-12-27
申请号：US981814
申请日：1992-11-25
申请人： Noubar B. Afeyan , Fred E. Regnier
发明人： Noubar B. Afeyan , Fred E. Regnier
IPC分类号： G01N33/561 , G01N27/447 , B01D57/02
CPC分类号： G01N27/44795 , G01N27/44747
摘要： Disclosed are methods and apparatus for determining the presence of one or more analytes in a sample, wherein the presence of a complex of an analyte and an analyte-specific binding moiety is detected at a location in an elongate pH gradient corresponding to a predetermined isoelectric point of the complex in the gradient. An electric field applied across the elongate pH gradient prior to the detection of the complex transports the complex to the location in the pH gradient corresponding to the predetermined isoelectric point. The analyte-specific binding moiety preferably is provided with a detectable label such as a fluorescent label. A parameter, e.g., fluorescence intensity, indicative of the amount of the complex at the location in the pH gradient corresponding to the predetermined isoelectric point may be determined to quantitate the analyte.
摘要翻译： 公开了用于确定样品中一种或多种分析物的存在的方法和装置，其中在对应于预定等电点的细长pH梯度中的位置处检测到分析物和分析物特异性结合部分的复合物的存在 的复合体在渐变。 在检测复合物之前跨越细长pH梯度施加的电场将络合物输送到对应于预定等电点的pH梯度中的位置。 分析物特异性结合部分优选地具有可检测标记如荧光标记。 可以确定指示对应于预定等电点的pH梯度中位置处的络合物的量的参数，例如荧光强度，以定量分析物。
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公开(公告)号：US5552041A
公开(公告)日：1996-09-03
申请号：US317161
申请日：1994-10-03
CPC分类号： B01J20/285 , B01D15/345 , B01J20/28092 , B01J20/28095 , B01J2220/54 , G01N2030/528
摘要： Disclosed are chromatography methods and matrix geometries which permit high resolution, high productivity separation of mixtures of solutes, particularly biological materials. The method involves passing fluids through specially designed chromatography matrices at high flow rates. The matrices define first and second interconnected sets of pores and a high surface area for solute interaction in fluid communication with the members of the second set of pores. The first and second sets of pores are embodied, for example, as the interstices among particles and throughpores within the particles. The pores are dimensioned such that, at achievable high fluid flow rates, convective flow occurs in both pore sets, and the convective flow rate exceeds the rate of solute diffusion in the second pore set. This approach couples convective and diffusive mass transport to and from the active surface and permits increases in fluid velocity without the normally expected bandspreading.
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公开(公告)号：US5384042A
公开(公告)日：1995-01-24
申请号：US14473
申请日：1993-05-10
CPC分类号： B01J20/285 , B01D15/345 , B01J20/28092 , B01J20/28095 , B01J2220/54 , G01N2030/528
摘要： Disclosed are chromatography methods and matrix geometries which permit high resolution, high productivity separation of mixtures of solutes, particularly biological materials. The method involves passing fluids through specially designed chromatography matrices at high flow rates. The matrices define first and second interconnected sets of pores and a high surface area for solute interaction in fluid communication with the members of the second set of pores. The first and second sets of pores are embodied, for example, as the interstices among particles and throughpores within the particles. The pores are dimensioned such that, at achievable high fluid flow rates, convective flow occurs in both pore sets, and the convective flow rate exceeds the rate of solute diffusion in the second pore set. This approach couples convective and diffusive mass transport to and from the active surface and permits increases in fluid velocity without the normally expected bandspreading.
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9.发明授权
公开(公告)号：US5234586A
公开(公告)日：1993-08-10
申请号：US5877
申请日：1993-01-15
申请人： Noubar B. Afeyan , Fred E. Regnier
发明人： Noubar B. Afeyan , Fred E. Regnier
IPC分类号： B01D15/08 , G01N27/447 , G01N30/14 , G01N30/46 , G01N30/52 , G01N30/62 , G01N30/80 , G01N30/82 , G01N30/86 , G01N30/88
CPC分类号： G01N30/461 , G01N27/44717 , G01N30/62 , B01D15/08 , G01N2030/143 , G01N2030/524 , G01N2030/625 , G01N2030/8813 , G01N2030/8831 , G01N30/82
摘要： Disclosed is a method and apparatus for the rapid identification of a solute of interest in an effluent stream. The method involves separation of the solutes in the effluent and identification of a particular solute of interest by its selective subtraction from the effluent stream.
摘要翻译： 公开了用于快速鉴定流出物流中感兴趣溶质的方法和装置。 该方法包括通过从流出物流中的选择性减法来分离流出物中的溶质并鉴定特定的感兴趣溶质。
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公开(公告)号：US5019270A
公开(公告)日：1991-05-28
申请号：US595661
申请日：1990-10-09
CPC分类号： B01J20/282 , B01D15/345 , B01J20/28095 , B01J2220/54 , G01N2030/528
摘要： Disclosed are chromatography methods and matrix geometries which permit high resolution, high productivity separation of mixtures of solutes, particularly biological materials. The method involves passing fluids through specially designed chromatography matrices at high flow rates. The matrices define first and second interconnected sets of pores and a high surface area for solute interaction in fluid communication with the members of the second set of pores. The first and second sets of pores are embodied, for example, as the interstices among particles and throughpores within the particles. The pores are dimensioned such that, at achievable high fluid flow rates, convective flow occurs in both pore sets, and the convective flow rate exceeds the rate of solute diffusion in the second pore set. This approach couples convective and diffusive mass transport to and from the active surface and permits increases in fluid velocity without the normally expected bandspreading.
摘要翻译： 公开了色谱方法和基质几何形状，其允许溶质，特别是生物材料的混合物的高分辨率，高生产率分离。 该方法包括以高流速将流体通过专门设计的色谱基质。 这些基质定义了第一和第二相互连接的孔组，以及用于与第二组孔的构件流体连通的溶质相互作用的高表面积。 第一和第二组孔体现为例如颗粒内的颗粒和透孔之间的间隙。 孔的尺寸使得在可实现的高流体流速下，在两个孔组中都发生对流，并且对流流速超过第二孔组中溶质扩散的速率。 这种方法将对流和扩散质量传输与活性表面相耦合，并允许流体速度的增加而没有通常预期的带扩展。
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