公开(公告)号：US20140134691A1

公开(公告)日：2014-05-15

申请号：US14118856

申请日：2012-05-25

Applicant: Novozymes A/S

Inventor： Peter Olsen ,  Lars Christensen ,  Steen Joergensen ,  Torsten Regueira ,  Bjarke Christensen ,  Brian Kobmann ,  Thomas Grotkjaer

IPC: C12P7/04 ,  C12P5/02

CPC classification number: C12P7/04 ,  C12N1/20 ,  C12P5/026

Abstract: Described herein are host cells comprising lactate dehydrogenase activity, lactaldehyde dehydrogenase activity, lactaldehyde reductase activity, propanediol dehydratase activity, and/or n-propanol dehydrogenase activity, wherein the cells are capable of producing n-propanol. Also described are methods of producing n-propanol comprising (a) cultivating the host cells having lactate dehydrogenase activity, lactaldehyde dehydrogenase activity, lactaldehyde reductase activity, propanediol dehydratase activity, and/or n-propanol dehydrogenase activity in a medium under suitable conditions to produce n-propanol; and (b) recovering the n-propanol. Methods of producing polypropylene from the recombinant n-propanol are also provided.

Abstract translation: 本文描述了包含乳酸脱氢酶活性，乳醛脱氢酶活性，乳醛还原酶活性，丙二醇脱水酶活性和/或正丙醇脱氢酶活性的宿主细胞，其中细胞能够产生正丙醇。 还描述了生产正丙醇的方法，其包括（a）在合适条件下培养具有乳酸脱氢酶活性，内醛脱氢酶活性，乳醛还原酶活性，丙二醇脱水酶活性和/或正丙醇脱氢酶活性的宿主细胞，以产生 正丙醇 和（b）回收正丙醇。 还提供了从重组正丙醇制备聚丙烯的方法。

公开(公告)号：US20150125959A1

公开(公告)日：2015-05-07

申请号：US14397512

申请日：2013-05-17

Applicant: Novozymes A/S

Inventor： Steen Troels Joergensen ,  Torsten Bak Regueira ,  Brian Kobmann ,  Peter Bjarke Olsen ,  Bjarke Christensen

IPC: C12N15/74

CPC classification number: C12N15/746 ,  C12N9/1007 ,  C12N9/22

Abstract: Provided herein are Lactobacillus mutants having improved transformation efficiency, comprising a disruption to an endogenous gene encoding a restriction modification system protein. Also described are methods for producing the mutants, methods for generating transformants using the mutants, and methods for producing a polypeptide or fermentation product using the mutants.

Abstract translation: 本文提供了具有改善的转化效率的乳杆菌突变体，其包含对编码限制性修饰系统蛋白质的内源基因的破坏。 还描述了用于产生突变体的方法，使用突变体产生转化体的方法，以及使用突变体产生多肽或发酵产物的方法。

公开(公告)号：US09803209B2

公开(公告)日：2017-10-31

申请号：US14397512

申请日：2013-05-17

Applicant: Novozymes A/S

Inventor： Steen Troels Joergensen ,  Torsten Bak Regueira ,  Brian Kobmann ,  Peter Bjarke Olsen ,  Bjarke Christensen

IPC: C12N15/74 ,  C12N9/10 ,  C12N9/22

CPC classification number: C12N15/746 ,  C12N9/1007 ,  C12N9/22

Abstract: Provided herein are Lactobacillus mutants having improved transformation efficiency, comprising a disruption to an endogenous gene encoding a restriction modification system protein. Also described are methods for producing the mutants, methods for generating transformants using the mutants, and methods for producing a polypeptide or fermentation product using the mutants.