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    Alpha-Amylase Mutants
    2.
    发明申请
    Alpha-Amylase Mutants 审中-公开
    α-淀粉酶突变体

    公开(公告)号：US20130059315A1

    公开(公告)日：2013-03-07

    申请号：US13660218

    申请日：2012-10-25

    Applicant: Novozymes A/S

    Inventor： Allan Svendsen ,  Henrik Bisgaard-Frantzen ,  Torben Vedel Borchert

    IPC: C12N9/28 ,  C12Q1/40 ,  C12N9/30 ,  C12N15/63 ,  C11D3/386 ,  C12N1/15 ,  C12S9/00 ,  C12S11/00 ,  C12P19/14 ,  G01N33/573 ,  C12N1/21

    CPC classification number: C11D3/386 ,  C12N9/2417

    Abstract: The present invention relates to a method of constructing a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and at least one altered property as compared to the parent alpha-amylase, comprises i) analyzing the structure of the parent Termamyl-like alpha-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like alpha-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like alpha-amylase (as evaluated on the basis of structural or functional considerations), ii) constructing a Termamyl-like alpha-amylase variant, which as compared to the parent Termamyl-like alpha-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and, optionally, iii) testing the resulting Termamyl-like alpha-amylase variant with respect to the property in question.

    Abstract translation: 本发明涉及一种构建母体类戊酰样α-淀粉酶的变体的方法，该变体具有与母体α-淀粉酶相比具有α-淀粉酶活性和至少一种改变的性质，包括：i）分析 鉴定至少一个氨基酸残基或类甲酰样α-淀粉酶结构的至少一个结构部分，所述氨基酸残基或结构部分被认为与改变其性质有关的相关性 母体类戊酰样α-淀粉酶（根据结构或功能考虑进行评估），ii）构建与母体类戊酰样α-淀粉酶相比的类戊酰样α-淀粉酶变体，已经在 在i）中鉴定的氨基酸残基或结构部分，以改变其性质，以及任选地，iii）相对于所讨论的性质测试所得的类戊酰样α-淀粉酶变体。

    ALPHA-AMYLASE MUTANTS
    6.
    发明申请
    ALPHA-AMYLASE MUTANTS 审中-公开

    公开(公告)号：US20160222365A1

    公开(公告)日：2016-08-04

    申请号：US15012229

    申请日：2016-02-01

    Applicant: NOVOZYMES A/S

    Inventor： Torben Vedel Borchert ,  Allan Svendsen ,  Carsten Andersen ,  Bjarne Nielsen ,  Torben Lauesgaard Nissen ,  Soren Kjaerulff

    IPC: C12N9/28

    Abstract: The invention relates to a variant of a parent Termamyl-like α-amylase, which exhibits an alteration in at least one of the following properties relative to said parent α-amylase: i) improved pH stability at a pH from 8 to 10.5; and/or ii) improved Ca2+ stability at pH 8 to 10.5, and/or iii) increased specific activity at temperatures from 10 to 60° C.

    Abstract translation: 本发明涉及相对于所述亲本α-淀粉酶显示出至少一种以下特性的母体类戊酰样α-淀粉酶的变体：i）在8至10.5的pH下改善的pH稳定性; 和/或ii）在pH8至10.5时提高的Ca 2+稳定性，和/或iii）在10至60℃的温度下提高比活性。

    AMYLASE VARIANTS
    9.
    发明申请
    AMYLASE VARIANTS 审中-公开

    公开(公告)号：US20150080283A1

    公开(公告)日：2015-03-19

    申请号：US14553767

    申请日：2014-11-25

    Applicant: NOVOZYMES A/S

    Inventor： Henrik Bisgaard-Frantzen ,  Allan Svendsen ,  Torben Vedel Borchert

    IPC: C11D3/386 ,  C12N9/28

    CPC classification number: C11D3/38618 ,  C11D3/38609 ,  C11D3/38681 ,  C12N9/2417 ,  C12Y302/01001 ,  D06L1/14

    Abstract: The present invention relates to variants of a parent α-amylase, which parent α-amylase (i) has an amino acid sequence selected from the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, and SEQ ID No. 7, respectively; or (ii) displays at least 80% homology with one or more of these amino acid sequences; and/or displays immunological cross-reactivity with an antibody raised against an α-amylase having one of these amino acid sequences; and/or is encoded by a DNA sequence which hybridizes with the same probe as a DNA sequence encoding an α-amylase having one of these amino acid sequences; in which variant:(a) at least one amino acid residue of the parent α-amylase has been deleted; and/or (b) at least one amino acid residue of the parent α-amylase has been replaced by a different amino acid residue; and/or (c) at least one amino acid residue has been inserted relative to the parent α-amylase; the variant having α-amylase activity and exhibiting at least one of the following properties relative to the parent α-amylase: increased thermostability; increased stability towards oxidation; and reduced Ca2+ dependency; with the proviso that the amino acid sequence of the variant is not identical to any of the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3 and SEQ ID No. 7, respectively.

    Abstract translation: 本发明涉及母体α-淀粉酶的变体，其亲本α-淀粉酶（i）具有选自SEQ ID No.1，SEQ ID No.2，SEQ ID No.2所示的氨基酸序列的氨基酸序列。 3和SEQ ID No.7; 或（ii）与这些氨基酸序列中的一个或多个显示至少80％的同源性; 和/或显示与针对具有这些氨基酸序列之一的α-淀粉酶产生的抗体的免疫交叉反应性; 和/或由与编码具有这些氨基酸序列之一的α-淀粉酶的DNA序列相同的探针杂交的DNA序列编码; 在该变体中：（a）母体α-淀粉酶的至少一个氨基酸残基已被缺失; 和/或（b）母体α-淀粉酶的至少一个氨基酸残基被不同的氨基酸残基替代; 和/或（c）相对于母体α-淀粉酶插入至少一个氨基酸残基; 该变体具有α-淀粉酶活性并且相对于母体α-淀粉酶表现出以下性质中的至少一个：增加的热稳定性; 提高氧化稳定性; 并减少Ca2 +依赖; 条件是变体的氨基酸序列分别与SEQ ID No.1，SEQ ID No.2，SEQ ID No.3和SEQ ID No.7所示的任何氨基酸序列不同。

    Alpha-Amylase Mutants
    10.
    发明申请
    Alpha-Amylase Mutants 审中-公开
    α-淀粉酶突变体

    公开(公告)号：US20140024083A1

    公开(公告)日：2014-01-23

    申请号：US13953409

    申请日：2013-07-29

    Applicant: NOVOZYMES A/S

    Inventor： Allan Svendsen ,  Torben Vedel Borchert ,  Henrik Bisgaard-Frantzen

    IPC: C12N9/28 ,  C12P19/14

    CPC classification number: C12N9/2417 ,  C11D3/386 ,  C12P19/02 ,  C12P19/14

    Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to the parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency and specific activity.

    Abstract translation: 本发明涉及母体类戊酰样α-淀粉酶的变体，该变体具有α-淀粉酶活性并且相对于母体α-淀粉酶在下列特性中表现出至少一个变化：底物特异性，底物结合，底物 裂解模式，热稳定性，pH /活性分布，pH /稳定性分布，对氧化的稳定性，Ca 2+依赖性和比活性。

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