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    METHOD FOR PRODUCING STEM CELL-DERIVED LACRIMAL GLAND TISSUE
    1.
    发明申请
    METHOD FOR PRODUCING STEM CELL-DERIVED LACRIMAL GLAND TISSUE 有权

    公开(公告)号：US20210024896A1

    公开(公告)日：2021-01-28

    申请号：US16981455

    申请日：2019-03-28

    Applicant: Osaka University

    Inventor： Kohji Nishida ,  Ryuhei Hayashi ,  Toru Okubo ,  Yoichi Honma

    IPC: C12N5/074 ,  A61K35/55

    Abstract: Provided is a method for producing a stem cell-derived lacrimal gland tissue, the method comprising isolating SSEA4 and CD104 double positive cells from a self-formed ectodermal autonomous multi-zone (SEAM) cell population derived from pluripotent stem cells and three-dimensionally culturing the isolated cells in a medium with epidermal growth factor (EGF) and a ROCK inhibitor to produce a cell population expressing a lacrimal gland-related protein. The present invention provides a lacrimal gland organoid produced from pluripotent stem cells including iPS cells and thus is very useful for cell-based regenerative therapy for lacrimal gland-related diseases and cell-based research on the diseases.

    METHOD FOR CONTROLLING DIFFERENTIATION OF PLURIPOTENT STEM CELLS
    2.
    发明申请
    METHOD FOR CONTROLLING DIFFERENTIATION OF PLURIPOTENT STEM CELLS 审中-公开

    公开(公告)号：US20200010800A1

    公开(公告)日：2020-01-09

    申请号：US16482160

    申请日：2018-01-31

    Applicant: Osaka University

    Inventor： Kohji Nishida ,  Kiyotoshi Sekiguchi ,  Ryuhei Hayashi ,  Shun Shibata

    IPC: C12N5/079

    Abstract: The present invention relates to a method for controlling differentiation of pluripotent stem cells, which method comprises selecting a laminin or a fragment thereof based on binding affinity for the pluripotent stem cells and inducing differentiation of the pluripotent stem cells in the presence of the laminin or a fragment thereof. Here, the binding affinity for cells can be assessed by time-course observation of the survival rate and motility of the cells. According to the present invention, a cell population containing any desired proportion of differentiated cells can be produced from pluripotent stem cells in a simple manner. The cell population obtained by this production method is very useful for cell therapy-based treatment strategies for diseases.

    TISSUE-PRESERVING LIQUID AND TISSUE-PRESERVING METHOD
    4.
    发明申请
    TISSUE-PRESERVING LIQUID AND TISSUE-PRESERVING METHOD 审中-公开
    组织保存液体和组织保存方法

    公开(公告)号：US20150351380A1

    公开(公告)日：2015-12-10

    申请号：US14416148

    申请日：2013-07-03

    Applicant: OSAKA UNIVERSITY

    Inventor： Ryuhei Hayashi ,  Kohji Nishida ,  Ryosuke Katori

    IPC: A01N1/02

    CPC classification number: A01N1/0226

    Abstract: The present invention provides a tissue preservation solution that has good quality for preservation of tissues, organs and cells, and is useful in the fields of regenerative medicine, transplantation medicine, etc. The tissue preservation solution of the present invention comprises a compound having a Nrf2 activating effect. Preferable compounds having a Nrf2 activating effect are electrophiles, and in particular, ebselen and tert-butylhydroquinone. A preferable basal solution of the tissue preservation solution is Hank's balanced salt solution.

    Abstract translation: 本发明提供一种保存组织，器官和细胞的良好质量的组织保存液，可用于再生医学，移植药物等领域。本发明的组织保存液含有具有Nrf2 激活效果。 具有Nrf2活化作用的优选化合物是亲电子试剂，尤其是依普硒和叔丁基氢醌。 组织保存液的优选基础溶液是汉克平衡盐溶液。

    METHOD FOR PRODUCING A CORNEAL EPITHELIAL CELL POPULATION
    7.
    发明申请
    METHOD FOR PRODUCING A CORNEAL EPITHELIAL CELL POPULATION 有权

    公开(公告)号：US20210332324A1

    公开(公告)日：2021-10-28

    申请号：US16476521

    申请日：2017-12-27

    Applicant: Osaka University

    Inventor： Kohji Nishida ,  Ryuhei Hayashi ,  Shun Shibata

    IPC: C12N5/079 ,  A61L27/38

    Abstract: The present invention provides a method for producing a corneal epithelial cell population for the fabrication of corneal epithelial cell sheets for transplantation, the method comprising the steps of: (1) preparing a cell population containing corneal epithelial cells; (2) subjecting the cell population prepared in step (1) to magnetic-activated cell sorting and collecting CD200-negative/SSEA-4-positive cells; and (3) bringing the cells collected in step (2) into contact with a laminin selected from the group consisting of a laminin having an α3 chain, a laminin having an α4 chain and a laminin having an α5 chain, or an E8 fragment thereof, and collecting cells adherent thereto.

    METHOD FOR INDUCING DIFFERENTIATION INTO PLURIPOTENT STEM CELL-DERIVED CONJUNCTIVAL CELLS
    8.
    发明申请
    METHOD FOR INDUCING DIFFERENTIATION INTO PLURIPOTENT STEM CELL-DERIVED CONJUNCTIVAL CELLS 有权

    公开(公告)号：US20210207087A1

    公开(公告)日：2021-07-08

    申请号：US17057021

    申请日：2019-05-22

    Applicant: Osaka University

    Inventor： Kohji Nishida ,  Ryuhei Hayashi ,  Kimihito Nomi

    IPC: C12N5/079 ,  G01N33/50

    Abstract: Provided is a method for inducing differentiation into conjunctival epithelial cells, conjunctival goblet cells, and conjunctival epithelial stem and progenitor cells, the method comprising culturing colonies of pluripotent stem cells in a medium containing an epidermal growth factor (EGF) signaling activator to induce differentiation of the pluripotent stem cells. The present invention enables differentiation of pluripotent stem cells including iPS cells into conjunctival epithelial cells, conjunctival goblet cells, and conjunctival epithelial stem and progenitor cells and therefore is very useful for, for example, basic research on conjunctival epithelial cells and conjunctival goblet cells, regenerative therapy for intractable ocular surface diseases, and research related to the diseases.

    METHOD FOR DETERMINING ANATOMICAL SITES OF BODY SURFACE TISSUE
    9.
    发明申请
    METHOD FOR DETERMINING ANATOMICAL SITES OF BODY SURFACE TISSUE 审中-公开

    公开(公告)号：US20190390274A1

    公开(公告)日：2019-12-26

    申请号：US16480938

    申请日：2018-01-26

    Applicant: Osaka University

    Inventor： Kohji Nishida ,  Ryuhei Hayashi ,  Toru Okubo

    IPC: C12Q1/6881

    Abstract: An object of the present invention is to provide a method for determining anatomical sites of a body surface tissue. Provided is a method for determining an anatomical site of origin of a body surface tissue specimen, the method comprising the steps of:(A) selecting at least 8 kinds of genes from the group consisting of a Hox gene family and a Pax6 gene; (B) measuring the expression levels of the genes selected in the above (A) in the body surface tissue specimen; and (C) determining the anatomical site of origin of the body surface tissue specimen based on the expression levels or a combination of the expression levels measured in the above (B).

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