公开(公告)号：US11066641B2

公开(公告)日：2021-07-20

申请号：US15542200

申请日：2016-01-13

Applicant: Osaka University

Inventor： Kohji Nishida ,  Ryuhei Hayashi ,  Yuki Ishikawa

IPC: C12N5/079 ,  C12N5/10 ,  C12N5/074

Abstract: The present invention relates to a method for inducing the differentiation of corneal epithelial cells from pluripotent stem cells. More specifically, the present invention relates to a method for autonomously differentiating pluripotent stem cells, such as human iPS cells, into ectodermal cell lineage in a serum-free medium without using feeder cells and inducing the differentiation of the resultant ocular surface ectodermal lineage cells into corneal epithelial cells.

公开(公告)号：US10968428B2

公开(公告)日：2021-04-06

申请号：US15508749

申请日：2017-03-03

Applicant: OSAKA UNIVERSITY

Inventor： Kohji Nishida ,  Motokazu Tsujikawa ,  Susumu Hara ,  Satoshi Kawasaki ,  Masahito Yoshihara ,  Masayoshi Itoh ,  Hideya Kawaji ,  Hiroko Ohmiya

IPC: C12N5/079 ,  C12Q1/68 ,  C12N15/09 ,  C12N5/10 ,  A61K35/30 ,  C12N15/10 ,  C12N15/67 ,  C12Q1/686 ,  G01N33/569 ,  A61L27/00

Abstract: A molecular marker expressed specifically in corneal endothelial cells, and a method for producing one or more corneal endothelial cells using the marker and a method for evaluating one or more corneal endothelial cells using the marker are provided. At least one molecule selected from the group consisting of ZP4, MRGPRX3, GRIP1, GLP1R, HTR1D, and CLRN1 is used as a marker specific to corneal endothelial cells.

公开(公告)号：US11357798B2

公开(公告)日：2022-06-14

申请号：US15749766

申请日：2016-08-03

Applicant: OSAKA UNIVERSITY ,  ROHTO PHARMACEUTICAL CO., LTD.

Inventor： Kohji Nishida ,  Ryuuhei Hayashi ,  Yoichi Honma ,  Toru Okubo ,  Shun Shibata

IPC: A61K35/28 ,  A61P27/02 ,  C12N5/0775 ,  C12N5/00 ,  C12Q1/02 ,  A61K35/12

Abstract: The present invention relates to mesenchymal stem cell-derived microparticles having activity that promotes the growth of corneal epithelial stem cells and/or corneal epithelial cells, activity that maintains corneal epithelial stem cells in an undifferentiated state or promotes the formation of colonies thereby, and function that protects corneal epithelium.

公开(公告)号：US20210024896A1

公开(公告)日：2021-01-28

申请号：US16981455

申请日：2019-03-28

Applicant: Osaka University

Inventor： Kohji Nishida ,  Ryuhei Hayashi ,  Toru Okubo ,  Yoichi Honma

IPC: C12N5/074 ,  A61K35/55

Abstract: Provided is a method for producing a stem cell-derived lacrimal gland tissue, the method comprising isolating SSEA4 and CD104 double positive cells from a self-formed ectodermal autonomous multi-zone (SEAM) cell population derived from pluripotent stem cells and three-dimensionally culturing the isolated cells in a medium with epidermal growth factor (EGF) and a ROCK inhibitor to produce a cell population expressing a lacrimal gland-related protein. The present invention provides a lacrimal gland organoid produced from pluripotent stem cells including iPS cells and thus is very useful for cell-based regenerative therapy for lacrimal gland-related diseases and cell-based research on the diseases.

公开(公告)号：US20200010800A1

公开(公告)日：2020-01-09

申请号：US16482160

申请日：2018-01-31

Applicant: Osaka University

Inventor： Kohji Nishida ,  Kiyotoshi Sekiguchi ,  Ryuhei Hayashi ,  Shun Shibata

IPC: C12N5/079

Abstract: The present invention relates to a method for controlling differentiation of pluripotent stem cells, which method comprises selecting a laminin or a fragment thereof based on binding affinity for the pluripotent stem cells and inducing differentiation of the pluripotent stem cells in the presence of the laminin or a fragment thereof. Here, the binding affinity for cells can be assessed by time-course observation of the survival rate and motility of the cells. According to the present invention, a cell population containing any desired proportion of differentiated cells can be produced from pluripotent stem cells in a simple manner. The cell population obtained by this production method is very useful for cell therapy-based treatment strategies for diseases.

公开(公告)号：US12272459B2

公开(公告)日：2025-04-08

申请号：US17493856

申请日：2021-10-05

Applicant: Topcon Corporation ,  Osaka University

Inventor： Kohji Nishida ,  Atsuya Miki ,  Kazuichi Maruyama ,  Ryo Kawasaki ,  Song Mei ,  Zaixing Mao ,  Zhenguo Wang ,  Kinpui Chan ,  Xin Sui

IPC: G06T7/12 ,  G06T7/10 ,  G06T15/08 ,  G16H50/20 ,  G16H50/30

Abstract: A medical diagnostic apparatus includes a receiver circuit that receives three-dimensional data of a subject's eye, and a processor configured to separate portions of the three-dimensional data into separate segments, perform processing differently on each of the separate segments, and combine the separately processed segments to produce a segmented three-dimensional data set. The processor is further configured to generate at least one diagnostic metric from the segmented three-dimensional data set, and the processor is further configured to evaluate a pathological condition based on the at least one diagnostic metric. Related methods and computer readable media are also disclosed.

公开(公告)号：US10232153B2

公开(公告)日：2019-03-19

申请号：US15126553

申请日：2015-02-26

Applicant: OSAKA UNIVERSITY ,  HOYA CORPORATION

Inventor： Kohji Nishida ,  Takeshi Soma ,  Yusuke Goto ,  Kikuo Mitomo

IPC: A61M31/00 ,  A61F2/00 ,  A61F2/14 ,  A61F9/007 ,  A61F9/00

Abstract: A therapeutic instrument (20), includes: a support member (25) having a tongue-shaped supporting portion (50) for supporting a sheet-like therapeutic agent; a nozzle member (23) having a cylindrical portion (30) in which the supporting portion (50) supporting the therapeutic agent can be housed, and having an opening (34) through which the supporting portion (50) can be loaded and unloaded in/from a tip of the cylindrical portion (30); a syringe unit (22) that acts a positive pressure in the cylindrical portion (30) for pushing-out the therapeutic agent housed in the cylindrical portion (30) together with the supporting portion (50) to outside the cylindrical portion (30) through the opening (34); and a water flow forming part including a plurality of protrusions (53) formed on a surface of the supporting portion (50) so as to form a water flow at a place where the supporting portion (50) and the therapeutic agent are facing each other, when a medical water is fed into the cylindrical portion (30) in a state in which the therapeutic agent is housed in the cylindrical portion (30) together with the supporting portion (50).

公开(公告)号：US20150351380A1

公开(公告)日：2015-12-10

申请号：US14416148

申请日：2013-07-03

Applicant: OSAKA UNIVERSITY

Inventor： Ryuhei Hayashi ,  Kohji Nishida ,  Ryosuke Katori

IPC: A01N1/02

CPC classification number: A01N1/0226

Abstract: The present invention provides a tissue preservation solution that has good quality for preservation of tissues, organs and cells, and is useful in the fields of regenerative medicine, transplantation medicine, etc. The tissue preservation solution of the present invention comprises a compound having a Nrf2 activating effect. Preferable compounds having a Nrf2 activating effect are electrophiles, and in particular, ebselen and tert-butylhydroquinone. A preferable basal solution of the tissue preservation solution is Hank's balanced salt solution.

Abstract translation: 本发明提供一种保存组织，器官和细胞的良好质量的组织保存液，可用于再生医学，移植药物等领域。本发明的组织保存液含有具有Nrf2 激活效果。 具有Nrf2活化作用的优选化合物是亲电子试剂，尤其是依普硒和叔丁基氢醌。 组织保存液的优选基础溶液是汉克平衡盐溶液。

公开(公告)号：US20150087624A1

公开(公告)日：2015-03-26

申请号：US14395952

申请日：2013-04-23

Applicant: OSAKA UNIVERSITY

Inventor： Koichi Baba ,  Kohji Nishida ,  Noriyasu Hashida

IPC: A61K9/10 ,  A61K31/57 ,  A61K9/00 ,  A61K31/27

CPC classification number: A61K9/10 ,  A61K9/0014 ,  A61K9/0048 ,  A61K9/19 ,  A61K31/27 ,  A61K31/57 ,  A61K47/44 ,  A61K51/082 ,  B82Y5/00 ,  B82Y40/00

Abstract: A nanoparticle aqueous dispersion in which nanoparticles are dispersed in water is produced through a method including a step of freeze-drying a frozen sample of a liquid mixture of a first solution and a second solution and a step of dispersing the freeze-dried sample in water. In this method, the liquid mixture contains an active ingredient and an ointment base, the first solution includes contains an organic solvent as its solvent, and the second solution contains water as its solvent. The method, which is arranged as such, can provide an aqueous composition containing nanoparticles dispersed therein and usable stably as an aqueous dispersion preparation.

Abstract translation: 通过包括冷冻干燥第一溶液和第二溶液的液体混合物的冷冻样品的步骤的方法制备纳米颗粒分散在水中的纳米颗粒水分散体，以及将冷冻干燥样品分散在水中的步骤 。 在该方法中，液体混合物含有活性成分和软膏基质，第一溶液包含有机溶剂作为溶剂，第二溶液含有水作为溶​​剂。 这样布置的方法可以提供含有分散在其中的纳米颗粒的水性组合物，并可稳定地用作水分散体制剂。

公开(公告)号：US12247219B2

公开(公告)日：2025-03-11

申请号：US17348174

申请日：2021-06-15

Applicant: Osaka University

Inventor： Kohji Nishida ,  Ryuhei Hayashi ,  Yuki Ishikawa

IPC: C12N5/079 ,  C12N5/074 ,  C12N5/10

Abstract: The present invention relates to a method for inducing the differentiation of corneal epithelial cells from pluripotent stem cells. More specifically, the present invention relates to a method for autonomously differentiating pluripotent stem cells, such as human iPS cells, into ectodermal cell lineage in a serum-free medium without using feeder cells and inducing the differentiation of the resultant ocular surface ectodermal lineage cells into corneal epithelial cells.