公开(公告)号：US20190276804A1

公开(公告)日：2019-09-12

申请号：US16348944

申请日：2017-11-09

Applicant: OSAKA UNIVERSITY ,  KYOTO UNIVERSITY

Inventor： Kiyotoshi SEKIGUCHI ,  Fumi EBISU ,  Hidetoshi SAKURAI ,  Mingming ZHAO ,  Megumu SAITO

IPC: C12N5/077 ,  C12N5/071

Abstract: Provided is a method for inducing pluripotent stem cells to differentiate into somatic cells in a culture medium containing a heparin binding growth factor, the method comprising bringing cells into contact with a conjugate of a laminin E8 fragment and a growth factor binding domain-containing fragment of a heparan sulfate proteoglycan. According to the present invention, pluripotent stem cells can be induced to differentiate into any desired somatic cells in a highly efficient manner.

公开(公告)号：US20170370821A1

公开(公告)日：2017-12-28

申请号：US15543513

申请日：2016-01-14

Applicant: KYOTO UNIVERSITY

Inventor： Hirohide SAITO ,  Seiya TAKAHASHI ,  Hidetoshi SAKURAI ,  Takahiko SATO ,  Satoru TAKAYAMA

IPC: G01N15/14 ,  G01N21/64

CPC classification number: G01N15/1434 ,  C12N5/10 ,  G01N21/6428 ,  G01N2015/149 ,  G01N2021/6439

Abstract: The present invention aims to provide a method of sorting a skeletal muscle progenitor cell from a cell population containing the skeletal muscle progenitor cell. The above-mentioned problem is solved by providing a step of introducing miRNA-responsive mRNA into a cell population. The miRNA-responsive mRNA contains (i) a nucleic acid having a sequence specifically recognized by miRNA specifically expressed in a skeletal muscle progenitor cell, and (ii) a nucleic acid containing a sequence encoding a marker protein.

公开(公告)号：US20230256024A1

公开(公告)日：2023-08-17

申请号：US18014201

申请日：2021-07-13

Applicant: KYOTO UNIVERSITY ,  ASAHI KASEI KABUSHIKI KAISHA

Inventor： Hidetoshi SAKURAI ,  Mingming ZHAO ,  Hiroki KATO ,  Atsutoshi TAZUMI

IPC: A61K35/34 ,  C12N5/077

CPC classification number: A61K35/34 ,  C12N5/0658 ,  C12N2500/25 ,  C12N2501/12 ,  C12N2501/105 ,  C12N2501/115 ,  C12N2506/45

Abstract: The present invention provides a composition that is for treating myogenic diseases and that contains a cell group including skeletal muscle precursor cells differentiation-induced from pluripotent stem cells, the composition being characterized in that the cell group includes the skeletal muscle precursor cells that are Pax7-positive, and are CD146 (MCAM)-positive and CD57-negative on the surface thereof. The present invention also provides a method for purifying skeletal muscle precursor cells.