公开(公告)号：US11959103B2

公开(公告)日：2024-04-16

申请号：US16348944

申请日：2017-11-09

申请人： OSAKA UNIVERSITY ,  KYOTO UNIVERSITY

发明人： Kiyotoshi Sekiguchi ,  Fumi Ebisu ,  Hidetoshi Sakurai ,  Mingming Zhao ,  Megumu Saito

IPC分类号： C12N5/0735 ,  A61K35/34 ,  A61K35/545 ,  A61P9/00 ,  A61P21/00 ,  A61P43/00 ,  C12N5/071 ,  C12N5/077 ,  C12N5/10

CPC分类号： C12N5/0657 ,  A61K35/34 ,  A61K35/545 ,  A61P9/00 ,  A61P21/00 ,  A61P43/00 ,  C12N5/0658 ,  C12N5/069 ,  C12N5/10 ,  C12N2501/115 ,  C12N2501/12 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/165 ,  C12N2501/91 ,  C12N2501/998 ,  C12N2506/45

摘要： Provided is a method for inducing pluripotent stem cells to differentiate into somatic cells in a culture medium containing a heparin binding growth factor, the method comprising bringing cells into contact with a conjugate of a laminin E8 fragment and a growth factor binding domain-containing fragment of a heparan sulfate proteoglycan. According to the present invention, pluripotent stem cells can be induced to differentiate into any desired somatic cells in a highly efficient manner.