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公开(公告)号：US11357798B2
公开(公告)日：2022-06-14
申请号：US15749766
申请日：2016-08-03
Applicant: OSAKA UNIVERSITY , ROHTO PHARMACEUTICAL CO., LTD.
Inventor： Kohji Nishida , Ryuuhei Hayashi , Yoichi Honma , Toru Okubo , Shun Shibata
Abstract: The present invention relates to mesenchymal stem cell-derived microparticles having activity that promotes the growth of corneal epithelial stem cells and/or corneal epithelial cells, activity that maintains corneal epithelial stem cells in an undifferentiated state or promotes the formation of colonies thereby, and function that protects corneal epithelium.
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公开(公告)号：US20210332324A1
公开(公告)日：2021-10-28
申请号：US16476521
申请日：2017-12-27
Applicant: Osaka University
Inventor： Kohji Nishida , Ryuhei Hayashi , Shun Shibata
Abstract: The present invention provides a method for producing a corneal epithelial cell population for the fabrication of corneal epithelial cell sheets for transplantation, the method comprising the steps of: (1) preparing a cell population containing corneal epithelial cells; (2) subjecting the cell population prepared in step (1) to magnetic-activated cell sorting and collecting CD200-negative/SSEA-4-positive cells; and (3) bringing the cells collected in step (2) into contact with a laminin selected from the group consisting of a laminin having an α3 chain, a laminin having an α4 chain and a laminin having an α5 chain, or an E8 fragment thereof, and collecting cells adherent thereto.
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公开(公告)号：US11649433B2
公开(公告)日：2023-05-16
申请号：US16482160
申请日：2018-01-31
Applicant: Osaka University
Inventor： Kohji Nishida , Kiyotoshi Sekiguchi , Ryuhei Hayashi , Shun Shibata
IPC: A61K35/545 , C12N5/079 , A61K38/18
CPC classification number: C12N5/0621 , A61K35/545 , A61K38/1825 , A61K38/18 , C12N2501/998 , C12N2506/45 , C12N2533/52
Abstract: The present invention relates to a method for controlling differentiation of pluripotent stem cells, which method comprises selecting a laminin or a fragment thereof based on binding affinity for the pluripotent stem cells and inducing differentiation of the pluripotent stem cells in the presence of the laminin or a fragment thereof. Here, the binding affinity for cells can be assessed by time-course observation of the survival rate and motility of the cells. According to the present invention, a cell population containing any desired proportion of differentiated cells can be produced from pluripotent stem cells in a simple manner. The cell population obtained by this production method is very useful for cell therapy-based treatment strategies for diseases.
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公开(公告)号：US20200010800A1
公开(公告)日：2020-01-09
申请号：US16482160
申请日：2018-01-31
Applicant: Osaka University
Inventor： Kohji Nishida , Kiyotoshi Sekiguchi , Ryuhei Hayashi , Shun Shibata
IPC: C12N5/079
Abstract: The present invention relates to a method for controlling differentiation of pluripotent stem cells, which method comprises selecting a laminin or a fragment thereof based on binding affinity for the pluripotent stem cells and inducing differentiation of the pluripotent stem cells in the presence of the laminin or a fragment thereof. Here, the binding affinity for cells can be assessed by time-course observation of the survival rate and motility of the cells. According to the present invention, a cell population containing any desired proportion of differentiated cells can be produced from pluripotent stem cells in a simple manner. The cell population obtained by this production method is very useful for cell therapy-based treatment strategies for diseases.
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